Restriction map for
pFN21AE3615
(Linear) (Six-base) MAPSORT of: KIEE3615.seq Check: 2447 from: 1 to: 1020
KIEE3615.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AflIII A'CryG_T
Cuts at: 0 356 493 1020
Size: 356 137 527
Fragments arranged by size:
527 356 137
AhdI GACnn_n'nnGTC
Cuts at: 0 653 1020
Size: 653 367
AleI CACnn'nnGTG
Cuts at: 0 551 823 1020
Size: 551 272 197
Fragments arranged by size:
551 272 197
AlwNI CAG_nnn'CTG
Cuts at: 0 780 1020
Size: 780 240
ApaLI G'TGCA_C
Cuts at: 0 268 1020
Size: 268 752
AvaI C'yCGr_G
Cuts at: 0 480 1020
Size: 480 540
BanII G_rGCy'C
Cuts at: 0 733 1020
Size: 733 287
BbsI GAAGACnn'nnnn_
Cuts at: 0 535 1020
Size: 535 485
Bme1580I G_kGCm'C
Cuts at: 0 272 1020
Size: 272 748
BmgBI CAC'GTC
Cuts at: 0 821 1020
Size: 821 199
BmrI ACTGGGnnnn_n'
Cuts at: 0 195 527 1020
Size: 195 332 493
Fragments arranged by size:
493 332 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1020
Size: 25 995
BsaAI yAC'GTr
Cuts at: 0 494 1020
Size: 494 526
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 279 1020
Size: 279 741
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 1020
Size: 708 312
BsiHKAI G_wGCw'C
Cuts at: 0 272 1020
Size: 272 748
Bsp1286I G_dGCh'C
Cuts at: 0 272 733 1020
Size: 272 461 287
Fragments arranged by size:
461 287 272
BspHI T'CATG_A
Cuts at: 0 299 1020
Size: 299 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 658 770 1020
Size: 658 112 250
Fragments arranged by size:
658 250 112
BsrDI GCAATG_nn'
Cuts at: 0 399 888 1020
Size: 399 489 132
Fragments arranged by size:
489 399 132
BssSI C'ACGA_G
Cuts at: 0 673 796 1020
Size: 673 123 224
Fragments arranged by size:
673 224 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 809 1020
Size: 809 211
BtgI C'CryG_G
Cuts at: 0 824 1020
Size: 824 196
BtsI GCAGTG_nn'
Cuts at: 0 280 1020
Size: 280 740
DraIII CAC_nnn'GTG
Cuts at: 0 494 1020
Size: 494 526
EaeI y'GGCC_r
Cuts at: 0 164 669 843 927 980 1020
Size: 164 505 174 84 53 40
Fragments arranged by size:
505 174 164 84 53 40
EarI CTCTTCn'nnn_
Cuts at: 0 598 1020
Size: 598 422
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 159 1020
Size: 159 861
EcoO109I rG'GnC_Cy
Cuts at: 0 605 647 1020
Size: 605 42 373
Fragments arranged by size:
605 373 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 639 671 704 736 1020
Size: 85 32 522 32 33 32 284
Fragments arranged by size:
522 284 85 33 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1020
Size: 659 361
MscI TGG'CCA
Cuts at: 0 166 671 929 982 1020
Size: 166 505 258 53 38
Fragments arranged by size:
505 258 166 53 38
MslI CAynn'nnrTG
Cuts at: 0 551 823 1020
Size: 551 272 197
Fragments arranged by size:
551 272 197
MspA1I CmG'CkG
Cuts at: 0 964 1020
Size: 964 56
NspI r_CATG'y
Cuts at: 0 341 360 1020
Size: 341 19 660
Fragments arranged by size:
660 341 19
PciI A'CATG_T
Cuts at: 0 356 1020
Size: 356 664
PflMI CCAn_nnn'nTGG
Cuts at: 0 185 629 1020
Size: 185 444 391
Fragments arranged by size:
444 391 185
PfoI T'CCnGG_A
Cuts at: 0 452 1020
Size: 452 568
PmlI CAC'GTG
Cuts at: 0 494 1020
Size: 494 526
PpuMI rG'GwC_Cy
Cuts at: 0 647 1020
Size: 647 373
PstI C_TGCA'G
Cuts at: 0 814 1020
Size: 814 206
PvuII CAG'CTG
Cuts at: 0 964 1020
Size: 964 56
SfcI C'TryA_G
Cuts at: 0 810 1020
Size: 810 210
SmlI C'TyrA_G
Cuts at: 0 40 1020
Size: 40 980
SspI AAT'ATT
Cuts at: 0 468 1020
Size: 468 552
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 89 343 375 478 510 1020
Size: 57 32 254 32 103 32 510
Fragments arranged by size:
510 254 103 57 32 32 32
Enzymes that do cut:
AflIII AhdI AleI AlwNI ApaLI AvaI BanII BbsI
Bme1580I BmgBI BmrI BpuEI BsaAI BseRI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BtgI BtsI
DraIII EaeI EarI EciI EcoO109I Hin4I MmeI MscI
MslI MspA1I NspI PciI PflMI PfoI PmlI PpuMI
PstI PvuII SfcI SmlI SspI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI BplI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI StyI SwaI TaqII TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI