(Linear) (Six-base) MAPSORT of: KIEE3613.seq  Check: 9219  from: 1  to: 444



KIEE3613.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:36  ..

AccI GT'mk_AC

Cuts at:      0     137     444 
   Size:        137     307
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     285     444 
   Size:        285     159
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     346     444 
   Size:        346      98
 
AlwNI CAG_nnn'CTG

Cuts at:      0     308     444 
   Size:        308     136
 
ApaI G_GGCC'C

Cuts at:      0      12     444 
   Size:         12     432
 
AvaI C'yCGr_G

Cuts at:      0       5     444 
   Size:          5     439
 
BanI G'GyrC_C

Cuts at:      0      97     444 
   Size:         97     347
 
BanII G_rGCy'C

Cuts at:      0      12     116     444 
   Size:         12     104     328

  Fragments arranged by size: 

                 328     104      12
 
Bme1580I G_kGCm'C

Cuts at:      0      12     102     444 
   Size:         12      90     342

  Fragments arranged by size: 

                 342      90      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     120     183     444 
   Size:        120      63     261

  Fragments arranged by size: 

                 261     120      63
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     148     178     444 
   Size:        148      30     266

  Fragments arranged by size: 

                 266     148      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     444 
   Size:         94     350
 
BseYI C'CCAG_C

Cuts at:      0     237     444 
   Size:        237     207
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     444 
   Size:        198     246
 
BsiHKAI G_wGCw'C

Cuts at:      0     116     444 
   Size:        116     328
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     151     444 
   Size:        151     293
 
Bsp1286I G_dGCh'C

Cuts at:      0      12     102     116     444 
   Size:         12      90      14     328

  Fragments arranged by size: 

                 328      90      14      12
 
BspHI T'CATG_A

Cuts at:      0      65     444 
   Size:         65     379
 
BstEII G'GTnAC_C

Cuts at:      0     384     444 
   Size:        384      60
 
BtgI C'CryG_G

Cuts at:      0      25     444 
   Size:         25     419
 
BtsI GCAGTG_nn'

Cuts at:      0      71     290     444 
   Size:         71     219     154

  Fragments arranged by size: 

                 219     154      71
 
EaeI y'GGCC_r

Cuts at:      0     206     291     444 
   Size:        206      85     153

  Fragments arranged by size: 

                 206     153      85
 
EarI CTCTTCn'nnn_

Cuts at:      0      67     444 
   Size:         67     377
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     244     444 
   Size:        244     200
 
EcoICRI GAG'CTC

Cuts at:      0     114     444 
   Size:        114     330
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     285     444 
   Size:        285     159
 
EcoO109I rG'GnC_Cy

Cuts at:      0       9     336     444 
   Size:          9     327     108

  Fragments arranged by size: 

                 327     108       9
 
MslI CAynn'nnrTG

Cuts at:      0     382     444 
   Size:        382      62
 
MspA1I CmG'CkG

Cuts at:      0      27     444 
   Size:         27     417
 
PfoI T'CCnGG_A

Cuts at:      0     107     444 
   Size:        107     337
 
PpuMI rG'GwC_Cy

Cuts at:      0     336     444 
   Size:        336     108
 
PspOMI G'GGCC_C

Cuts at:      0       8     444 
   Size:          8     436
 
SacI G_AGCT'C

Cuts at:      0     116     444 
   Size:        116     328
 
SacII CC_GC'GG

Cuts at:      0      28     444 
   Size:         28     416
 
SanDI GG'GwC_CC

Cuts at:      0     336     444 
   Size:        336     108
 
SfcI C'TryA_G

Cuts at:      0     195     444 
   Size:        195     249
 
SmaI CCC'GGG

Cuts at:      0       7     444 
   Size:          7     437
 
SrfI GCCC'GGGC

Cuts at:      0       7     444 
   Size:          7     437
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     139     444 
   Size:        139     305
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     148     180     444 
   Size:        148      32     264

  Fragments arranged by size: 

                 264     148      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     261     444 
   Size:        261     183
 
XmaI C'CCGG_G

Cuts at:      0       5     444 
   Size:          5     439
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApaI     AvaI     BanI    BanII
 Bme1580I     BmrI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspHI   BstEII     BtgI     BtsI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     MslI   MspA1I     PfoI    PpuMI   PspOMI
     SacI    SacII    SanDI     SfcI     SmaI     SrfI    TaqII     TstI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsiEI    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmnI     ZraI