Restriction map for
pFN21AE3607
(Linear) (Six-base) MAPSORT of: KIEE3607.seq Check: 2572 from: 1 to: 753
KIEE3607.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 118 753
Size: 118 635
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 472 753
Size: 285 187 281
Fragments arranged by size:
285 281 187
AflIII A'CryG_T
Cuts at: 0 742 753
Size: 742 11
AlwNI CAG_nnn'CTG
Cuts at: 0 57 438 753
Size: 57 381 315
Fragments arranged by size:
381 315 57
ApoI r'AATT_y
Cuts at: 0 595 753
Size: 595 158
AseI AT'TA_AT
Cuts at: 0 206 753
Size: 206 547
BanI G'GyrC_C
Cuts at: 0 12 136 753
Size: 12 124 617
Fragments arranged by size:
617 124 12
BanII G_rGCy'C
Cuts at: 0 375 666 753
Size: 375 291 87
Fragments arranged by size:
375 291 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 199 384 753
Size: 199 185 369
Fragments arranged by size:
369 199 185
BciVI GTATCCnnnnn_n'
Cuts at: 0 312 753
Size: 312 441
BfrBI ATG'CAT
Cuts at: 0 347 753
Size: 347 406
BglII A'GATC_T
Cuts at: 0 152 753
Size: 152 601
BlpI GC'TnA_GC
Cuts at: 0 644 753
Size: 644 109
Bme1580I G_kGCm'C
Cuts at: 0 86 753
Size: 86 667
BmrI ACTGGGnnnn_n'
Cuts at: 0 45 567 753
Size: 45 522 186
Fragments arranged by size:
522 186 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 670 753
Size: 511 159 83
Fragments arranged by size:
511 159 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 753
Size: 359 394
BsaHI Gr'CG_yC
Cuts at: 0 521 753
Size: 521 232
BsaWI w'CCGG_w
Cuts at: 0 128 753
Size: 128 625
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 383 753
Size: 383 370
BseYI C'CCAG_C
Cuts at: 0 288 325 753
Size: 288 37 428
Fragments arranged by size:
428 288 37
BsiHKAI G_wGCw'C
Cuts at: 0 128 375 753
Size: 128 247 378
Fragments arranged by size:
378 247 128
BsmI GAATG_Cn'
Cuts at: 0 293 753
Size: 293 460
Bsp1286I G_dGCh'C
Cuts at: 0 86 128 375 666 753
Size: 86 42 247 291 87
Fragments arranged by size:
291 247 87 86 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 118 753
Size: 118 635
BsrDI GCAATG_nn'
Cuts at: 0 347 753
Size: 347 406
BstXI CCAn_nnnn'nTGG
Cuts at: 0 258 639 753
Size: 258 381 114
Fragments arranged by size:
381 258 114
BstYI r'GATC_y
Cuts at: 0 152 753
Size: 152 601
Bsu36I CC'TnA_GG
Cuts at: 0 58 753
Size: 58 695
BtgI C'CryG_G
Cuts at: 0 140 753
Size: 140 613
BtsI GCAGTG_nn'
Cuts at: 0 439 753
Size: 439 314
EaeI y'GGCC_r
Cuts at: 0 440 753
Size: 440 313
EarI CTCTTCn'nnn_
Cuts at: 0 487 753
Size: 487 266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 753
Size: 55 698
EcoICRI GAG'CTC
Cuts at: 0 373 753
Size: 373 380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 472 511 670 753
Size: 285 187 39 159 83
Fragments arranged by size:
285 187 159 83 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 61 753
Size: 61 692
EcoO109I rG'GnC_Cy
Cuts at: 0 177 753
Size: 177 576
EcoRI G'AATT_C
Cuts at: 0 595 753
Size: 595 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 137 169 397 429 753
Size: 137 32 228 32 324
Fragments arranged by size:
324 228 137 32 32
MslI CAynn'nnrTG
Cuts at: 0 169 256 753
Size: 169 87 497
Fragments arranged by size:
497 169 87
MspA1I CmG'CkG
Cuts at: 0 325 753
Size: 325 428
NsiI A_TGCA'T
Cuts at: 0 349 753
Size: 349 404
NspI r_CATG'y
Cuts at: 0 746 753
Size: 746 7
PciI A'CATG_T
Cuts at: 0 742 753
Size: 742 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 434 753
Size: 434 319
PpuMI rG'GwC_Cy
Cuts at: 0 177 753
Size: 177 576
SacI G_AGCT'C
Cuts at: 0 375 753
Size: 375 378
ScaI AGT'ACT
Cuts at: 0 556 753
Size: 556 197
SfcI C'TryA_G
Cuts at: 0 363 753
Size: 363 390
SmlI C'TyrA_G
Cuts at: 0 374 753
Size: 374 379
StyI C'CwwG_G
Cuts at: 0 158 753
Size: 158 595
TatI w'GTAC_w
Cuts at: 0 554 753
Size: 554 199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 255 435 753
Size: 165 90 180 318
Fragments arranged by size:
318 180 165 90
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApoI AseI BanI BanII
BbsI BciVI BfrBI BglII BlpI Bme1580I BmrI BpmI
BpuEI BsaHI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I
MslI MspA1I NsiI NspI PciI PflMI PpuMI SacI
ScaI SfcI SmlI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BglI
BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaXI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI