Restriction map for
pFN21AE3605
(Linear) (Six-base) MAPSORT of: KIEE3605.seq Check: 9168 from: 1 to: 1374
KIEE3605.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 893 1374
Size: 893 481
Acc65I G'GTAC_C
Cuts at: 0 1233 1374
Size: 1233 141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 255 572 1374
Size: 79 176 317 802
Fragments arranged by size:
802 317 176 79
AflIII A'CryG_T
Cuts at: 0 22 1118 1374
Size: 22 1096 256
Fragments arranged by size:
1096 256 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 155 187 1374
Size: 155 32 1187
Fragments arranged by size:
1187 155 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1077 1374
Size: 1077 297
ApaLI G'TGCA_C
Cuts at: 0 1270 1374
Size: 1270 104
ApoI r'AATT_y
Cuts at: 0 350 616 1374
Size: 350 266 758
Fragments arranged by size:
758 350 266
AvaI C'yCGr_G
Cuts at: 0 1138 1374
Size: 1138 236
AvrII C'CTAG_G
Cuts at: 0 49 1374
Size: 49 1325
BanI G'GyrC_C
Cuts at: 0 1233 1374
Size: 1233 141
BanII G_rGCy'C
Cuts at: 0 16 1182 1374
Size: 16 1166 192
Fragments arranged by size:
1166 192 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 272 1374
Size: 272 1102
BglI GCCn_nnn'nGGC
Cuts at: 0 318 1374
Size: 318 1056
BglII A'GATC_T
Cuts at: 0 896 1374
Size: 896 478
BlpI GC'TnA_GC
Cuts at: 0 1305 1374
Size: 1305 69
Bme1580I G_kGCm'C
Cuts at: 0 1274 1374
Size: 1274 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 423 823 943 1012 1220 1364 1374
Size: 417 6 400 120 69 208 144 10
Fragments arranged by size:
417 400 208 144 120 69 10 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1166 1374
Size: 944 222 208
Fragments arranged by size:
944 222 208
BsaWI w'CCGG_w
Cuts at: 0 264 1311 1374
Size: 264 1047 63
Fragments arranged by size:
1047 264 63
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 572 820 907 1305 1374
Size: 572 248 87 398 69
Fragments arranged by size:
572 398 248 87 69
BseYI C'CCAG_C
Cuts at: 0 483 1077 1252 1374
Size: 483 594 175 122
Fragments arranged by size:
594 483 175 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1149 1374
Size: 832 317 225
Fragments arranged by size:
832 317 225
BsiHKAI G_wGCw'C
Cuts at: 0 120 971 1028 1274 1374
Size: 120 851 57 246 100
Fragments arranged by size:
851 246 120 100 57
BsiWI C'GTAC_G
Cuts at: 0 227 1374
Size: 227 1147
Bsp1286I G_dGCh'C
Cuts at: 0 16 120 971 1028 1182 1274 1374
Size: 16 104 851 57 154 92 100
Fragments arranged by size:
851 154 104 100 92 57 16
BspEI T'CCGG_A
Cuts at: 0 264 1374
Size: 264 1110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 893 1245 1374
Size: 893 352 129
Fragments arranged by size:
893 352 129
BsrDI GCAATG_nn'
Cuts at: 0 787 1374
Size: 787 587
BsrGI T'GTAC_A
Cuts at: 0 1004 1219 1374
Size: 1004 215 155
Fragments arranged by size:
1004 215 155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 447 1374
Size: 447 927
BstYI r'GATC_y
Cuts at: 0 91 896 1374
Size: 91 805 478
Fragments arranged by size:
805 478 91
Bsu36I CC'TnA_GG
Cuts at: 0 890 1374
Size: 890 484
BtsI GCAGTG_nn'
Cuts at: 0 668 1374
Size: 668 706
EaeI y'GGCC_r
Cuts at: 0 242 1374
Size: 242 1132
EarI CTCTTCn'nnn_
Cuts at: 0 120 1374
Size: 120 1254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 255 417 423 572 823 943 1012
Size: 79 176 162 6 149 251 120 69
Cuts at: 1012 1220 1364 1374
Size: 208 144 10
Fragments arranged by size:
251 208 176 162 149 144 120 79
69 10 6
EcoRI G'AATT_C
Cuts at: 0 350 1374
Size: 350 1024
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 883 1374
Size: 883 491
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 723 755 1374
Size: 723 32 619
Fragments arranged by size:
723 619 32
HincII GTy'rAC
Cuts at: 0 132 1374
Size: 132 1242
HindIII A'AGCT_T
Cuts at: 0 774 1277 1302 1374
Size: 774 503 25 72
Fragments arranged by size:
774 503 72 25
KpnI G_GTAC'C
Cuts at: 0 1237 1374
Size: 1237 137
MluI A'CGCG_T
Cuts at: 0 22 1374
Size: 22 1352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 1147 1374
Size: 261 886 227
Fragments arranged by size:
886 261 227
MscI TGG'CCA
Cuts at: 0 244 1374
Size: 244 1130
MspA1I CmG'CkG
Cuts at: 0 1074 1133 1152 1374
Size: 1074 59 19 222
Fragments arranged by size:
1074 222 59 19
NspI r_CATG'y
Cuts at: 0 1003 1122 1374
Size: 1003 119 252
Fragments arranged by size:
1003 252 119
PciI A'CATG_T
Cuts at: 0 1118 1374
Size: 1118 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 424 1077 1374
Size: 424 653 297
Fragments arranged by size:
653 424 297
PshAI GACnn'nnGTC
Cuts at: 0 1163 1374
Size: 1163 211
PstI C_TGCA'G
Cuts at: 0 904 1374
Size: 904 470
PvuII CAG'CTG
Cuts at: 0 1074 1133 1152 1374
Size: 1074 59 19 222
Fragments arranged by size:
1074 222 59 19
ScaI AGT'ACT
Cuts at: 0 276 794 1374
Size: 276 518 580
Fragments arranged by size:
580 518 276
SfcI C'TryA_G
Cuts at: 0 5 185 209 900 1374
Size: 5 180 24 691 474
Fragments arranged by size:
691 474 180 24 5
SmlI C'TyrA_G
Cuts at: 0 959 1181 1366 1374
Size: 959 222 185 8
Fragments arranged by size:
959 222 185 8
SpeI A'CTAG_T
Cuts at: 0 277 1374
Size: 277 1097
StyI C'CwwG_G
Cuts at: 0 49 500 1374
Size: 49 451 874
Fragments arranged by size:
874 451 49
TatI w'GTAC_w
Cuts at: 0 274 792 1004 1219 1374
Size: 274 518 212 215 155
Fragments arranged by size:
518 274 215 212 155
XbaI T'CTAG_A
Cuts at: 0 834 1374
Size: 834 540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 684 1294 1374
Size: 684 610 80
Fragments arranged by size:
684 610 80
Enzymes that do cut:
AarI Acc65I AcuI AflIII AloI AlwNI ApaLI ApoI
AvaI AvrII BanI BanII BciVI BglI BglII BlpI
Bme1580I BpmI BpuEI BsaWI BseRI BseYI BsgI BsiHKAI
BsiWI Bsp1286I BspEI BspMI BsrDI BsrGI BstXI BstYI
Bsu36I BtsI EaeI EarI Eco57MI EcoRI FalI Hin4I
HincII HindIII KpnI MluI MmeI MscI MspA1I NspI
PciI PflMI PshAI PstI PvuII ScaI SfcI SmlI
SpeI StyI TatI XbaI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI ApaI AscI AseI AsiSI BaeI BamHI BbeI
BbsI BbvCI BcgI BclI BfrBI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BsiEI
BsmI BsmBI BspHI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI HaeII HpaI KasI MfeI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XhoI XmaI XmnI ZraI