Restriction map for
pFN21AE3604
(Linear) (Six-base) MAPSORT of: KIEE3604.seq Check: 5726 from: 1 to: 1062
KIEE3604.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AccI GT'mk_AC
Cuts at: 0 731 1062
Size: 731 331
Acc65I G'GTAC_C
Cuts at: 0 143 1062
Size: 143 919
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 23 820 1062
Size: 14 9 797 242
Fragments arranged by size:
797 242 14 9
AflIII A'CryG_T
Cuts at: 0 184 1062
Size: 184 878
AleI CACnn'nnGTG
Cuts at: 0 597 1062
Size: 597 465
AlwNI CAG_nnn'CTG
Cuts at: 0 578 1062
Size: 578 484
ApoI r'AATT_y
Cuts at: 0 18 530 655 1062
Size: 18 512 125 407
Fragments arranged by size:
512 407 125 18
BanI G'GyrC_C
Cuts at: 0 143 1062
Size: 143 919
BbsI GAAGACnn'nnnn_
Cuts at: 0 749 1062
Size: 749 313
BglI GCCn_nnn'nGGC
Cuts at: 0 136 155 1062
Size: 136 19 907
Fragments arranged by size:
907 136 19
Bme1580I G_kGCm'C
Cuts at: 0 184 1003 1062
Size: 184 819 59
Fragments arranged by size:
819 184 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 84 1062
Size: 84 978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 916 1003 1015 1062
Size: 448 468 87 12 47
Fragments arranged by size:
468 448 87 47 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 919 1062
Size: 919 143
BseYI C'CCAG_C
Cuts at: 0 307 899 905 1043 1062
Size: 307 592 6 138 19
Fragments arranged by size:
592 307 138 19 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1062
Size: 564 498
BsiEI CG_ry'CG
Cuts at: 0 731 1062
Size: 731 331
BsmBI CGTCTCn'nnnn_
Cuts at: 0 884 1062
Size: 884 178
Bsp1286I G_dGCh'C
Cuts at: 0 184 1003 1062
Size: 184 819 59
Fragments arranged by size:
819 184 59
BsrGI T'GTAC_A
Cuts at: 0 378 1062
Size: 378 684
BstYI r'GATC_y
Cuts at: 0 765 1062
Size: 765 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 220 1062
Size: 220 842
BtsI GCAGTG_nn'
Cuts at: 0 592 604 632 1062
Size: 592 12 28 430
Fragments arranged by size:
592 430 28 12
EaeI y'GGCC_r
Cuts at: 0 387 1062
Size: 387 675
EarI CTCTTCn'nnn_
Cuts at: 0 839 1062
Size: 839 223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 23 448 820 916 1003 1015 1062
Size: 14 9 425 372 96 87 12 47
Fragments arranged by size:
425 372 96 87 47 14 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 931 1062
Size: 931 131
EcoO109I rG'GnC_Cy
Cuts at: 0 915 1062
Size: 915 147
EcoRV GAT'ATC
Cuts at: 0 345 1062
Size: 345 717
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 105 1062
Size: 105 957
HaeII r_GCGC'y
Cuts at: 0 161 1062
Size: 161 901
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 757 783 789 815 1062
Size: 757 26 6 26 247
Fragments arranged by size:
757 247 26 26 6
HincII GTy'rAC
Cuts at: 0 732 1062
Size: 732 330
KpnI G_GTAC'C
Cuts at: 0 147 1062
Size: 147 915
MscI TGG'CCA
Cuts at: 0 389 1062
Size: 389 673
MslI CAynn'nnrTG
Cuts at: 0 597 1062
Size: 597 465
MspA1I CmG'CkG
Cuts at: 0 816 1062
Size: 816 246
NspI r_CATG'y
Cuts at: 0 1028 1062
Size: 1028 34
PflMI CCAn_nnn'nTGG
Cuts at: 0 762 1062
Size: 762 300
PfoI T'CCnGG_A
Cuts at: 0 761 1062
Size: 761 301
PpuMI rG'GwC_Cy
Cuts at: 0 915 1062
Size: 915 147
PstI C_TGCA'G
Cuts at: 0 446 599 1062
Size: 446 153 463
Fragments arranged by size:
463 446 153
PvuII CAG'CTG
Cuts at: 0 816 1062
Size: 816 246
SalI G'TCGA_C
Cuts at: 0 730 1062
Size: 730 332
SfcI C'TryA_G
Cuts at: 0 442 595 1062
Size: 442 153 467
Fragments arranged by size:
467 442 153
SphI G_CATG'C
Cuts at: 0 1028 1062
Size: 1028 34
TatI w'GTAC_w
Cuts at: 0 378 861 1062
Size: 378 483 201
Fragments arranged by size:
483 378 201
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 71 1062
Size: 71 991
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlwNI ApoI BanI
BbsI BglI Bme1580I BmrI BpmI BseRI BseYI BsgI
BsiEI BsmBI Bsp1286I BsrGI BstYI BtgZI BtsI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII KpnI MscI MslI MspA1I NspI PflMI PfoI
PpuMI PstI PvuII SalI SfcI SphI TatI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiHKAI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI FseI FspI FspAI HindIII HpaI KasI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI XmnI ZraI