Restriction map for
pFN21AE3602
(Linear) (Six-base) MAPSORT of: KIEE3602.seq Check: 8303 from: 1 to: 1485
KIEE3602.orf
With 182 enzymes: SgfI *
March 22, 2012 16:37 ..
AatII G_ACGT'C
Cuts at: 0 95 1485
Size: 95 1390
AccI GT'mk_AC
Cuts at: 0 704 1485
Size: 704 781
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 936 1365 1485
Size: 295 641 429 120
Fragments arranged by size:
641 429 295 120
AflIII A'CryG_T
Cuts at: 0 149 1485
Size: 149 1336
AhdI GACnn_n'nnGTC
Cuts at: 0 641 1485
Size: 641 844
AleI CACnn'nnGTG
Cuts at: 0 817 1297 1485
Size: 817 480 188
Fragments arranged by size:
817 480 188
AlwNI CAG_nnn'CTG
Cuts at: 0 221 753 1015 1104 1221 1224 1388 1485
Size: 221 532 262 89 117 3 164 97
Fragments arranged by size:
532 262 221 164 117 97 89 3
ApaI G_GGCC'C
Cuts at: 0 1179 1485
Size: 1179 306
ApaLI G'TGCA_C
Cuts at: 0 467 1456 1485
Size: 467 989 29
Fragments arranged by size:
989 467 29
AvaI C'yCGr_G
Cuts at: 0 480 826 1157 1485
Size: 480 346 331 328
Fragments arranged by size:
480 346 331 328
BanI G'GyrC_C
Cuts at: 0 610 1161 1485
Size: 610 551 324
Fragments arranged by size:
610 551 324
BanII G_rGCy'C
Cuts at: 0 950 1146 1179 1485
Size: 950 196 33 306
Fragments arranged by size:
950 306 196 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 990 1485
Size: 990 495
BbvCI CC'TCA_GC
Cuts at: 0 650 1485
Size: 650 835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 396 430 1062 1096 1485
Size: 396 34 632 34 389
Fragments arranged by size:
632 396 389 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 59 1183 1485
Size: 59 1124 302
Fragments arranged by size:
1124 302 59
BglII A'GATC_T
Cuts at: 0 933 1485
Size: 933 552
BlpI GC'TnA_GC
Cuts at: 0 1145 1485
Size: 1145 340
Bme1580I G_kGCm'C
Cuts at: 0 323 343 471 768 1179 1375 1460 1485
Size: 323 20 128 297 411 196 85 25
Fragments arranged by size:
411 323 297 196 128 85 25 20
BmgBI CAC'GTC
Cuts at: 0 152 1485
Size: 152 1333
BmrI ACTGGGnnnn_n'
Cuts at: 0 438 598 1003 1485
Size: 438 160 405 482
Fragments arranged by size:
482 438 405 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 550 730 754 1485
Size: 231 319 180 24 731
Fragments arranged by size:
731 319 231 180 24
Bpu10I CC'TnA_GC
Cuts at: 0 650 1485
Size: 650 835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1485
Size: 97 1388
BsaHI Gr'CG_yC
Cuts at: 0 92 1485
Size: 92 1393
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1369 1399 1485
Size: 1369 30 86
Fragments arranged by size:
1369 86 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 951 1485
Size: 951 534
BseYI C'CCAG_C
Cuts at: 0 506 551 860 1015 1304 1485
Size: 506 45 309 155 289 181
Fragments arranged by size:
506 309 289 181 155 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1485
Size: 1234 251
BsiHKAI G_wGCw'C
Cuts at: 0 419 471 1460 1485
Size: 419 52 989 25
Fragments arranged by size:
989 419 52 25
Bsp1286I G_dGCh'C
Cuts at: 0 323 343 419 471 768 950 1146 1179
Size: 323 20 76 52 297 182 196 33
Cuts at: 1179 1375 1460 1485
Size: 196 85 25
Fragments arranged by size:
323 297 196 196 182 85 76 52
33 25 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 533 1485
Size: 533 952
BsrFI r'CCGG_y
Cuts at: 0 58 174 560 1485
Size: 58 116 386 925
Fragments arranged by size:
925 386 116 58
BssHII G'CGCG_C
Cuts at: 0 1251 1485
Size: 1251 234
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1221 1485
Size: 1221 264
BstXI CCAn_nnnn'nTGG
Cuts at: 0 603 1485
Size: 603 882
BstYI r'GATC_y
Cuts at: 0 933 1485
Size: 933 552
BtgI C'CryG_G
Cuts at: 0 47 574 596 777 854 1085 1286 1485
Size: 47 527 22 181 77 231 201 199
Fragments arranged by size:
527 231 201 199 181 77 47 22
BtsI GCAGTG_nn'
Cuts at: 0 19 431 918 1124 1292 1304 1485
Size: 19 412 487 206 168 12 181
Fragments arranged by size:
487 412 206 181 168 19 12
EaeI y'GGCC_r
Cuts at: 0 2 41 172 571 687 729 758 796
Size: 2 39 131 399 116 42 29 38
Cuts at: 796 1088 1184 1485
Size: 292 96 301
Fragments arranged by size:
399 301 292 131 116 96 42 39
38 29 2
EarI CTCTTCn'nnn_
Cuts at: 0 485 955 1414 1485
Size: 485 470 459 71
Fragments arranged by size:
485 470 459 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1452 1485
Size: 1452 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 295 550 730 754 936 1365 1485
Size: 231 64 255 180 24 182 429 120
Fragments arranged by size:
429 255 231 182 180 120 64 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 1485
Size: 301 1184
EcoO109I rG'GnC_Cy
Cuts at: 0 741 1485
Size: 741 744
FspI TGC'GCA
Cuts at: 0 1366 1485
Size: 1366 119
HaeII r_GCGC'y
Cuts at: 0 310 1312 1485
Size: 310 1002 173
Fragments arranged by size:
1002 310 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 627 659 1128 1160 1369 1401 1485
Size: 627 32 469 32 209 32 84
Fragments arranged by size:
627 469 209 84 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 1485
Size: 270 1215
MscI TGG'CCA
Cuts at: 0 760 1090 1485
Size: 760 330 395
Fragments arranged by size:
760 395 330
MslI CAynn'nnrTG
Cuts at: 0 154 424 817 1210 1297 1485
Size: 154 270 393 393 87 188
Fragments arranged by size:
393 393 270 188 154 87
MspA1I CmG'CkG
Cuts at: 0 33 49 367 649 675 864 1104 1149
Size: 33 16 318 282 26 189 240 45
Cuts at: 1149 1221 1288 1485
Size: 72 67 197
Fragments arranged by size:
318 282 240 197 189 72 67 45
33 26 16
NaeI GCC'GGC
Cuts at: 0 60 176 1485
Size: 60 116 1309
Fragments arranged by size:
1309 116 60
NcoI C'CATG_G
Cuts at: 0 596 854 1485
Size: 596 258 631
Fragments arranged by size:
631 596 258
NgoMIV G'CCGG_C
Cuts at: 0 58 174 1485
Size: 58 116 1311
Fragments arranged by size:
1311 116 58
NspI r_CATG'y
Cuts at: 0 1323 1485
Size: 1323 162
PflMI CCAn_nnn'nTGG
Cuts at: 0 1015 1485
Size: 1015 470
PfoI T'CCnGG_A
Cuts at: 0 95 128 1485
Size: 95 33 1357
Fragments arranged by size:
1357 95 33
PpuMI rG'GwC_Cy
Cuts at: 0 741 1485
Size: 741 744
PspOMI G'GGCC_C
Cuts at: 0 1175 1485
Size: 1175 310
PstI C_TGCA'G
Cuts at: 0 1100 1299 1485
Size: 1100 199 186
Fragments arranged by size:
1100 199 186
PvuII CAG'CTG
Cuts at: 0 649 675 1104 1149 1221 1485
Size: 649 26 429 45 72 264
Fragments arranged by size:
649 429 264 72 45 26
SacII CC_GC'GG
Cuts at: 0 50 1289 1485
Size: 50 1239 196
Fragments arranged by size:
1239 196 50
SapI GCTCTTCn'nnn_
Cuts at: 0 955 1414 1485
Size: 955 459 71
Fragments arranged by size:
955 459 71
SexAI A'CCwGG_T
Cuts at: 0 737 1485
Size: 737 748
SfcI C'TryA_G
Cuts at: 0 1096 1295 1485
Size: 1096 199 190
Fragments arranged by size:
1096 199 190
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 59 1183 1485
Size: 59 1124 302
Fragments arranged by size:
1124 302 59
SmlI C'TyrA_G
Cuts at: 0 112 1485
Size: 112 1373
StuI AGG'CCT
Cuts at: 0 403 1485
Size: 403 1082
StyI C'CwwG_G
Cuts at: 0 596 854 1485
Size: 596 258 631
Fragments arranged by size:
631 596 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 498 543 724 829 1485
Size: 498 45 181 105 656
Fragments arranged by size:
656 498 181 105 45
Tth111I GACn'n_nGTC
Cuts at: 0 739 1485
Size: 739 746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 286 417 525 1485
Size: 286 131 108 960
Fragments arranged by size:
960 286 131 108
ZraI GAC'GTC
Cuts at: 0 93 1485
Size: 93 1392
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BbvCI BcgI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrFI BssHII BstAPI BstXI BstYI BtgI BtsI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PflMI PfoI PpuMI PspOMI PstI PvuII SacII SapI
SexAI SfcI SfiI SmlI StuI StyI TaqII Tth111I
XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BciVI BclI BfrBI BmtI BplI BsaI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI XbaI XhoI
XmaI XmnI