(Linear) (Six-base) MAPSORT of: KIEE3594.seq  Check: 8209  from: 1  to: 522



KIEE3594.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     407     522 
   Size:        407     115
 
ApoI r'AATT_y

Cuts at:      0     439     522 
   Size:        439      83
 
BanI G'GyrC_C

Cuts at:      0     100     522 
   Size:        100     422
 
BbeI G_GCGC'C

Cuts at:      0     104     522 
   Size:        104     418
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     334     522 
   Size:        334     188
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     522 
   Size:        253     269
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     522 
   Size:        113     409
 
BsaHI Gr'CG_yC

Cuts at:      0      55     101     522 
   Size:         55      46     421

  Fragments arranged by size: 

                 421      55      46
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     522 
   Size:        250     272
 
BsiHKAI G_wGCw'C

Cuts at:      0     117     522 
   Size:        117     405
 
BsmI GAATG_Cn'

Cuts at:      0     266     522 
   Size:        266     256
 
Bsp1286I G_dGCh'C

Cuts at:      0     117     522 
   Size:        117     405
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     407     522 
   Size:        407     115
 
BsrBI CCG'CTC

Cuts at:      0      63     522 
   Size:         63     459
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     226     378     522 
   Size:        226     152     144

  Fragments arranged by size: 

                 226     152     144
 
BstBI TT'CG_AA

Cuts at:      0     325     522 
   Size:        325     197
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     231     522 
   Size:        231     291
 
Bsu36I CC'TnA_GG

Cuts at:      0     215     275     522 
   Size:        215      60     247

  Fragments arranged by size: 

                 247     215      60
 
BtgI C'CryG_G

Cuts at:      0      50     490     522 
   Size:         50     440      32

  Fragments arranged by size: 

                 440      50      32
 
EaeI y'GGCC_r

Cuts at:      0      47     522 
   Size:         47     475
 
EarI CTCTTCn'nnn_

Cuts at:      0     412     522 
   Size:        412     110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     522 
   Size:        253     269
 
EcoRI G'AATT_C

Cuts at:      0     439     522 
   Size:        439      83
 
HaeII r_GCGC'y

Cuts at:      0     104     522 
   Size:        104     418
 
KasI G'GCGC_C

Cuts at:      0     100     522 
   Size:        100     422
 
MslI CAynn'nnrTG

Cuts at:      0     401     522 
   Size:        401     121
 
MspA1I CmG'CkG

Cuts at:      0      52     522 
   Size:         52     470
 
NarI GG'CG_CC

Cuts at:      0     101     522 
   Size:        101     421
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     496     522 
   Size:        496      26
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     137     169     522 
   Size:        137      32     353

  Fragments arranged by size: 

                 353     137      32
 
PstI C_TGCA'G

Cuts at:      0     386     522 
   Size:        386     136
 
SacII CC_GC'GG

Cuts at:      0      53     522 
   Size:         53     469
 
SfcI C'TryA_G

Cuts at:      0     154     382     522 
   Size:        154     228     140

  Fragments arranged by size: 

                 228     154     140
 
SfoI GGC'GCC

Cuts at:      0     102     522 
   Size:        102     420
 
SmlI C'TyrA_G

Cuts at:      0     128     522 
   Size:        128     394
 
SspI AAT'ATT

Cuts at:      0     122     522 
   Size:        122     400
 
XmnI GAAnn'nnTTC

Cuts at:      0     421     522 
   Size:        421     101
 

 Enzymes that do cut:

     AarI     ApoI     BanI     BbeI     BbsI     BpmI    BpuEI    BsaHI
    BseRI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI   BstAPI    BstBI
    BstXI   Bsu36I     BtgI     EaeI     EarI  Eco57MI    EcoRI    HaeII
     KasI     MslI   MspA1I     NarI    PflMI     PsrI     PstI    SacII
     SfcI     SfoI     SmlI     SspI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstEII
    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI