(Linear) (Six-base) MAPSORT of: KIEE3593.seq  Check: 6800  from: 1  to: 459



KIEE3593.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:35  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     459 
   Size:         25     434
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     169     201     459 
   Size:        169      32     258

  Fragments arranged by size: 

                 258     169      32
 
ApoI r'AATT_y

Cuts at:      0     205     459 
   Size:        205     254
 
BanII G_rGCy'C

Cuts at:      0     359     459 
   Size:        359     100
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     191     459 
   Size:        191     268
 
BclI T'GATC_A

Cuts at:      0     200     459 
   Size:        200     259
 
BglII A'GATC_T

Cuts at:      0     218     459 
   Size:        218     241
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     334     459 
   Size:         52     282     125

  Fragments arranged by size: 

                 282     125      52
 
BsaWI w'CCGG_w

Cuts at:      0     396     459 
   Size:        396      63
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     169     199     439     459 
   Size:        169      30     240      20

  Fragments arranged by size: 

                 240     169      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103     459 
   Size:        103     356
 
BsmI GAATG_Cn'

Cuts at:      0     420     459 
   Size:        420      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     359     459 
   Size:        359     100
 
BspEI T'CCGG_A

Cuts at:      0     396     459 
   Size:        396      63
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      71     164     389     459 
   Size:         71      93     225      70

  Fragments arranged by size: 

                 225      93      71      70
 
BsrFI r'CCGG_y

Cuts at:      0     449     459 
   Size:        449      10
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      96     459 
   Size:         96     363
 
BstYI r'GATC_y

Cuts at:      0      36     218     459 
   Size:         36     182     241

  Fragments arranged by size: 

                 241     182      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      95     459 
   Size:         95     364
 
BtsI GCAGTG_nn'

Cuts at:      0     279     459 
   Size:        279     180
 
EarI CTCTTCn'nnn_

Cuts at:      0     416     459 
   Size:        416      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25      52     334     459 
   Size:         25      27     282     125

  Fragments arranged by size: 

                 282     125      27      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0     318     459 
   Size:        318     141
 
FspI TGC'GCA

Cuts at:      0     160     459 
   Size:        160     299
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     332     364     378     410     439     459 
   Size:        332      32      14      32      29      20

  Fragments arranged by size: 

                 332      32      32      29      20      14
 
MslI CAynn'nnrTG

Cuts at:      0      16     459 
   Size:         16     443
 
NspI r_CATG'y

Cuts at:      0      15     459 
   Size:         15     444
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     312     459 
   Size:        312     147
 
PfoI T'CCnGG_A

Cuts at:      0     311     459 
   Size:        311     148
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     169     201     459 
   Size:        169      32     258

  Fragments arranged by size: 

                 258     169      32
 
SphI G_CATG'C

Cuts at:      0      15     459 
   Size:         15     444
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     357     459 
   Size:        357     102
 
XmnI GAAnn'nnTTC

Cuts at:      0     148     459 
   Size:        148     311
 

 Enzymes that do cut:

     AcuI     AloI     ApoI    BanII    BciVI     BclI    BglII     BpmI
    BsaWI    BsaXI    BseRI     BsmI Bsp1286I    BspEI    BspMI    BsrFI
    BstXI    BstYI    BtgZI     BtsI     EarI  Eco57MI EcoO109I     FspI
    Hin4I     MslI     NspI    PflMI     PfoI     PpiI     SphI    TaqII
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI