Restriction map for
pFN21AE3589
(Linear) (Six-base) MAPSORT of: KIEE3589.seq Check: 8416 from: 1 to: 681
KIEE3589.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 653 681
Size: 118 535 28
Fragments arranged by size:
535 118 28
ApaI G_GGCC'C
Cuts at: 0 45 681
Size: 45 636
ApoI r'AATT_y
Cuts at: 0 421 681
Size: 421 260
AvaI C'yCGr_G
Cuts at: 0 45 51 681
Size: 45 6 630
Fragments arranged by size:
630 45 6
BanI G'GyrC_C
Cuts at: 0 61 681
Size: 61 620
BanII G_rGCy'C
Cuts at: 0 45 52 681
Size: 45 7 629
Fragments arranged by size:
629 45 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 536 681
Size: 536 145
BclI T'GATC_A
Cuts at: 0 32 681
Size: 32 649
Bme1580I G_kGCm'C
Cuts at: 0 45 64 148 350 681
Size: 45 19 84 202 331
Fragments arranged by size:
331 202 84 45 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 243 470 681
Size: 243 227 211
Fragments arranged by size:
243 227 211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 681
Size: 93 588
BsaHI Gr'CG_yC
Cuts at: 0 7 681
Size: 7 674
BsaWI w'CCGG_w
Cuts at: 0 589 681
Size: 589 92
BsmBI CGTCTCn'nnnn_
Cuts at: 0 493 681
Size: 493 188
Bsp1286I G_dGCh'C
Cuts at: 0 45 52 64 148 350 681
Size: 45 7 12 84 202 331
Fragments arranged by size:
331 202 84 45 12 7
BspEI T'CCGG_A
Cuts at: 0 589 681
Size: 589 92
BspHI T'CATG_A
Cuts at: 0 209 228 681
Size: 209 19 453
Fragments arranged by size:
453 209 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 681
Size: 128 553
BsrBI CCG'CTC
Cuts at: 0 28 681
Size: 28 653
BstXI CCAn_nnnn'nTGG
Cuts at: 0 441 681
Size: 441 240
EaeI y'GGCC_r
Cuts at: 0 402 681
Size: 402 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 118 653 681
Size: 93 25 535 28
Fragments arranged by size:
535 93 28 25
EcoO109I rG'GnC_Cy
Cuts at: 0 41 42 117 681
Size: 41 1 75 564
Fragments arranged by size:
564 75 41 1
EcoRI G'AATT_C
Cuts at: 0 421 681
Size: 421 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 681
Size: 17 32 632
Fragments arranged by size:
632 32 17
HindIII A'AGCT_T
Cuts at: 0 665 681
Size: 665 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 681
Size: 245 436
MspA1I CmG'CkG
Cuts at: 0 113 681
Size: 113 568
NspI r_CATG'y
Cuts at: 0 138 681
Size: 138 543
PfoI T'CCnGG_A
Cuts at: 0 125 548 681
Size: 125 423 133
Fragments arranged by size:
423 133 125
PpuMI rG'GwC_Cy
Cuts at: 0 117 681
Size: 117 564
PspOMI G'GGCC_C
Cuts at: 0 41 681
Size: 41 640
SmaI CCC'GGG
Cuts at: 0 47 681
Size: 47 634
SspI AAT'ATT
Cuts at: 0 556 681
Size: 556 125
StyI C'CwwG_G
Cuts at: 0 167 183 277 681
Size: 167 16 94 404
Fragments arranged by size:
404 167 94 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 262 681
Size: 262 419
XmaI C'CCGG_G
Cuts at: 0 45 681
Size: 45 636
Enzymes that do cut:
AcuI ApaI ApoI AvaI BanI BanII BbsI BclI
Bme1580I BmrI BpmI BsaHI BsaWI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BstXI EaeI Eco57MI EcoO109I EcoRI
Hin4I HindIII MmeI MspA1I NspI PfoI PpuMI PspOMI
SmaI SspI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI