Restriction map for
pFN21AE3582
(Linear) (Six-base) MAPSORT of: KIEE3582.seq Check: 3159 from: 1 to: 1674
KIEE3582.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AatII G_ACGT'C
Cuts at: 0 455 1674
Size: 455 1219
AccI GT'mk_AC
Cuts at: 0 338 739 1674
Size: 338 401 935
Fragments arranged by size:
935 401 338
Acc65I G'GTAC_C
Cuts at: 0 47 1674
Size: 47 1627
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 509 524 1674
Size: 102 407 15 1150
Fragments arranged by size:
1150 407 102 15
AflII C'TTAA_G
Cuts at: 0 511 1674
Size: 511 1163
AflIII A'CryG_T
Cuts at: 0 785 1674
Size: 785 889
AgeI A'CCGG_T
Cuts at: 0 314 1674
Size: 314 1360
AhdI GACnn_n'nnGTC
Cuts at: 0 1071 1674
Size: 1071 603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 746 1058 1674
Size: 746 312 616
Fragments arranged by size:
746 616 312
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 10 42 235 267 589 621 1674
Size: 10 32 193 32 322 32 1053
Fragments arranged by size:
1053 322 193 32 32 32 10
AlwNI CAG_nnn'CTG
Cuts at: 0 36 1032 1674
Size: 36 996 642
Fragments arranged by size:
996 642 36
AvaI C'yCGr_G
Cuts at: 0 14 239 601 891 1674
Size: 14 225 362 290 783
Fragments arranged by size:
783 362 290 225 14
BamHI G'GATC_C
Cuts at: 0 702 1674
Size: 702 972
BanI G'GyrC_C
Cuts at: 0 47 1192 1674
Size: 47 1145 482
Fragments arranged by size:
1145 482 47
BanII G_rGCy'C
Cuts at: 0 900 1346 1634 1674
Size: 900 446 288 40
Fragments arranged by size:
900 446 288 40
BglII A'GATC_T
Cuts at: 0 1490 1674
Size: 1490 184
BmgBI CAC'GTC
Cuts at: 0 189 1674
Size: 189 1485
BmrI ACTGGGnnnn_n'
Cuts at: 0 18 1674
Size: 18 1656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 78 1674
Size: 78 1596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 955 1330 1674
Size: 673 282 375 344
Fragments arranged by size:
673 375 344 282
Bpu10I CC'TnA_GC
Cuts at: 0 1580 1674
Size: 1580 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1396 1583 1674
Size: 1396 187 91
Fragments arranged by size:
1396 187 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 643 1674
Size: 643 1031
BsaAI yAC'GTr
Cuts at: 0 300 861 1674
Size: 300 561 813
Fragments arranged by size:
813 561 300
BsaHI Gr'CG_yC
Cuts at: 0 452 928 1674
Size: 452 476 746
Fragments arranged by size:
746 476 452
BsaWI w'CCGG_w
Cuts at: 0 314 407 1674
Size: 314 93 1267
Fragments arranged by size:
1267 314 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 235 265 1454 1484 1674
Size: 235 30 1189 30 190
Fragments arranged by size:
1189 235 190 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1342 1674
Size: 1342 332
BseYI C'CCAG_C
Cuts at: 0 590 966 1235 1548 1674
Size: 590 376 269 313 126
Fragments arranged by size:
590 376 313 269 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 841 1018 1674
Size: 280 561 177 656
Fragments arranged by size:
656 561 280 177
BsiHKAI G_wGCw'C
Cuts at: 0 59 1346 1487 1599 1674
Size: 59 1287 141 112 75
Fragments arranged by size:
1287 141 112 75 59
BsmI GAATG_Cn'
Cuts at: 0 666 1674
Size: 666 1008
BsmBI CGTCTCn'nnnn_
Cuts at: 0 661 1674
Size: 661 1013
Bsp1286I G_dGCh'C
Cuts at: 0 59 900 1346 1487 1599 1634 1674
Size: 59 841 446 141 112 35 40
Fragments arranged by size:
841 446 141 112 59 40 35
BspEI T'CCGG_A
Cuts at: 0 407 1674
Size: 407 1267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 1040 1674
Size: 83 957 634
Fragments arranged by size:
957 634 83
BsrBI CCG'CTC
Cuts at: 0 7 62 246 413 1674
Size: 7 55 184 167 1261
Fragments arranged by size:
1261 184 167 55 7
BsrDI GCAATG_nn'
Cuts at: 0 49 572 620 848 1674
Size: 49 523 48 228 826
Fragments arranged by size:
826 523 228 49 48
BsrFI r'CCGG_y
Cuts at: 0 314 1674
Size: 314 1360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 174 228 585 1287 1674
Size: 174 54 357 702 387
Fragments arranged by size:
702 387 357 174 54
BstYI r'GATC_y
Cuts at: 0 702 1268 1490 1674
Size: 702 566 222 184
Fragments arranged by size:
702 566 222 184
BtgI C'CryG_G
Cuts at: 0 141 1674
Size: 141 1533
BtsI GCAGTG_nn'
Cuts at: 0 719 1599 1674
Size: 719 880 75
Fragments arranged by size:
880 719 75
EaeI y'GGCC_r
Cuts at: 0 2 212 695 833 1158 1277 1674
Size: 2 210 483 138 325 119 397
Fragments arranged by size:
483 397 325 210 138 119 2
EarI CTCTTCn'nnn_
Cuts at: 0 91 528 1674
Size: 91 437 1146
Fragments arranged by size:
1146 437 91
EcoICRI GAG'CTC
Cuts at: 0 1344 1674
Size: 1344 330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 509 524 673 955 1330 1674
Size: 102 407 15 149 282 375 344
Fragments arranged by size:
407 375 344 282 149 102 15
EcoO109I rG'GnC_Cy
Cuts at: 0 18 487 532 1126 1302 1371 1674
Size: 18 469 45 594 176 69 303
Fragments arranged by size:
594 469 303 176 69 45 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 123 1674
Size: 123 1551
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1057 1089 1454 1486 1674
Size: 1057 32 365 32 188
Fragments arranged by size:
1057 365 188 32 32
HincII GTy'rAC
Cuts at: 0 366 1674
Size: 366 1308
KpnI G_GTAC'C
Cuts at: 0 51 1674
Size: 51 1623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 578 1674
Size: 503 75 1096
Fragments arranged by size:
1096 503 75
MscI TGG'CCA
Cuts at: 0 214 1160 1279 1674
Size: 214 946 119 395
Fragments arranged by size:
946 395 214 119
MslI CAynn'nnrTG
Cuts at: 0 1198 1674
Size: 1198 476
MspA1I CmG'CkG
Cuts at: 0 594 885 1560 1674
Size: 594 291 675 114
Fragments arranged by size:
675 594 291 114
NcoI C'CATG_G
Cuts at: 0 141 1674
Size: 141 1533
NspI r_CATG'y
Cuts at: 0 789 1168 1674
Size: 789 379 506
Fragments arranged by size:
789 506 379
PciI A'CATG_T
Cuts at: 0 785 1674
Size: 785 889
PflMI CCAn_nnn'nTGG
Cuts at: 0 1136 1674
Size: 1136 538
PmlI CAC'GTG
Cuts at: 0 300 861 1674
Size: 300 561 813
Fragments arranged by size:
813 561 300
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 1674
Size: 235 32 1407
Fragments arranged by size:
1407 235 32
PpuMI rG'GwC_Cy
Cuts at: 0 18 487 532 1371 1674
Size: 18 469 45 839 303
Fragments arranged by size:
839 469 303 45 18
PstI C_TGCA'G
Cuts at: 0 41 714 1674
Size: 41 673 960
Fragments arranged by size:
960 673 41
PvuII CAG'CTG
Cuts at: 0 594 885 1560 1674
Size: 594 291 675 114
Fragments arranged by size:
675 594 291 114
SacI G_AGCT'C
Cuts at: 0 1346 1674
Size: 1346 328
SanDI GG'GwC_CC
Cuts at: 0 18 487 1674
Size: 18 469 1187
Fragments arranged by size:
1187 469 18
SexAI A'CCwGG_T
Cuts at: 0 173 1674
Size: 173 1501
SfcI C'TryA_G
Cuts at: 0 37 710 1674
Size: 37 673 964
Fragments arranged by size:
964 673 37
SmaI CCC'GGG
Cuts at: 0 16 241 1674
Size: 16 225 1433
Fragments arranged by size:
1433 225 16
SmlI C'TyrA_G
Cuts at: 0 511 1375 1598 1674
Size: 511 864 223 76
Fragments arranged by size:
864 511 223 76
SphI G_CATG'C
Cuts at: 0 1168 1674
Size: 1168 506
StyI C'CwwG_G
Cuts at: 0 141 698 1188 1466 1674
Size: 141 557 490 278 208
Fragments arranged by size:
557 490 278 208 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 342 795 983 1674
Size: 342 453 188 691
Fragments arranged by size:
691 453 342 188
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1094 1126 1674
Size: 1094 32 548
Fragments arranged by size:
1094 548 32
XmaI C'CCGG_G
Cuts at: 0 14 239 1674
Size: 14 225 1435
Fragments arranged by size:
1435 225 14
XmnI GAAnn'nnTTC
Cuts at: 0 391 1674
Size: 391 1283
ZraI GAC'GTC
Cuts at: 0 453 1674
Size: 453 1221
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII AgeI AhdI
AlfI AloI AlwNI AvaI BamHI BanI BanII BglII
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstXI
BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
FalI Hin4I HincII KpnI MmeI MscI MslI MspA1I
NcoI NspI PciI PflMI PmlI PpiI PpuMI PstI
PvuII SacI SanDI SexAI SfcI SmaI SmlI SphI
StyI TaqII TstI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AleI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmtI BsaBI
BsiEI BsiWI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI TatI Tth111I XbaI XcmI XhoI