Restriction map for
pFN21AE3581
(Linear) (Six-base) MAPSORT of: KIEE3581.seq Check: 3432 from: 1 to: 3243
KIEE3581.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 522 1383 3243
Size: 522 861 1860
Fragments arranged by size:
1860 861 522
AatII G_ACGT'C
Cuts at: 0 1798 3091 3243
Size: 1798 1293 152
Fragments arranged by size:
1798 1293 152
AccI GT'mk_AC
Cuts at: 0 1421 2653 3243
Size: 1421 1232 590
Fragments arranged by size:
1421 1232 590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 844 934 1039 1177 2572 3243
Size: 555 289 90 105 138 1395 671
Fragments arranged by size:
1395 671 555 289 138 105 90
AfeI AGC'GCT
Cuts at: 0 660 757 3243
Size: 660 97 2486
Fragments arranged by size:
2486 660 97
AflIII A'CryG_T
Cuts at: 0 134 2573 3243
Size: 134 2439 670
Fragments arranged by size:
2439 670 134
AhdI GACnn_n'nnGTC
Cuts at: 0 222 3243
Size: 222 3021
AleI CACnn'nnGTG
Cuts at: 0 233 328 676 3243
Size: 233 95 348 2567
Fragments arranged by size:
2567 348 233 95
AlwNI CAG_nnn'CTG
Cuts at: 0 164 476 1155 1770 2843 3243
Size: 164 312 679 615 1073 400
Fragments arranged by size:
1073 679 615 400 312 164
ApaLI G'TGCA_C
Cuts at: 0 691 3243
Size: 691 2552
AvaI C'yCGr_G
Cuts at: 0 3097 3243
Size: 3097 146
BanI G'GyrC_C
Cuts at: 0 21 500 3243
Size: 21 479 2743
Fragments arranged by size:
2743 479 21
BanII G_rGCy'C
Cuts at: 0 122 155 563 989 1180 1202 1696 2047
Size: 122 33 408 426 191 22 494 351
Cuts at: 2047 2785 3243
Size: 738 458
Fragments arranged by size:
738 494 458 426 408 351 191 122
33 22
BbeI G_GCGC'C
Cuts at: 0 25 3243
Size: 25 3218
BbsI GAAGACnn'nnnn_
Cuts at: 0 326 812 2916 3110 3212 3243
Size: 326 486 2104 194 102 31
Fragments arranged by size:
2104 486 326 194 102 31
BbvCI CC'TCA_GC
Cuts at: 0 2645 3243
Size: 2645 598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1929 1963 3243
Size: 1929 34 1280
Fragments arranged by size:
1929 1280 34
BclI T'GATC_A
Cuts at: 0 881 3243
Size: 881 2362
BfrBI ATG'CAT
Cuts at: 0 1123 2799 3243
Size: 1123 1676 444
Fragments arranged by size:
1676 1123 444
BglI GCCn_nnn'nGGC
Cuts at: 0 2287 3243
Size: 2287 956
BlpI GC'TnA_GC
Cuts at: 0 2054 3243
Size: 2054 1189
Bme1580I G_kGCm'C
Cuts at: 0 142 695 1337 3243
Size: 142 553 642 1906
Fragments arranged by size:
1906 642 553 142
BmgBI CAC'GTC
Cuts at: 0 1358 2349 2432 2576 3243
Size: 1358 991 83 144 667
Fragments arranged by size:
1358 991 667 144 83
BmrI ACTGGGnnnn_n'
Cuts at: 0 2718 3243
Size: 2718 525
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1764 3243
Size: 1764 1479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 997 1003 2656 2701 2790 3243
Size: 364 633 6 1653 45 89 453
Fragments arranged by size:
1653 633 453 364 89 45 6
Bpu10I CC'TnA_GC
Cuts at: 0 1454 2645 2779 3178 3243
Size: 1454 1191 134 399 65
Fragments arranged by size:
1454 1191 399 134 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 232 286 727 1779 3243
Size: 232 54 441 1052 1464
Fragments arranged by size:
1464 1052 441 232 54
BsaAI yAC'GTr
Cuts at: 0 2969 3243
Size: 2969 274
BsaBI GATnn'nnATC
Cuts at: 0 2800 3243
Size: 2800 443
BsaHI Gr'CG_yC
Cuts at: 0 22 809 1282 1795 2656 3088 3243
Size: 22 787 473 513 861 432 155
Fragments arranged by size:
861 787 513 473 432 155 22
BsaWI w'CCGG_w
Cuts at: 0 3201 3243
Size: 3201 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 361 391 406 436 3243
Size: 166 30 165 30 15 30 2807
Fragments arranged by size:
2807 166 165 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 112 304 414 1498 1846 1888 2442
Size: 21 91 192 110 1084 348 42 554
Cuts at: 2442 2653 2662 3243
Size: 211 9 581
Fragments arranged by size:
1084 581 554 348 211 192 110 91
42 21 9
BseYI C'CCAG_C
Cuts at: 0 169 562 991 1628 1642 1783 2011 2699
Size: 169 393 429 637 14 141 228 688
Cuts at: 2699 3243
Size: 544
Fragments arranged by size:
688 637 544 429 393 228 169 141
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 601 3243
Size: 397 204 2642
Fragments arranged by size:
2642 397 204
BsiEI CG_ry'CG
Cuts at: 0 191 2334 3243
Size: 191 2143 909
Fragments arranged by size:
2143 909 191
BsiHKAI G_wGCw'C
Cuts at: 0 155 695 989 1180 1202 3243
Size: 155 540 294 191 22 2041
Fragments arranged by size:
2041 540 294 191 155 22
BsmI GAATG_Cn'
Cuts at: 0 2529 3243
Size: 2529 714
BsmBI CGTCTCn'nnnn_
Cuts at: 0 697 1273 3243
Size: 697 576 1970
Fragments arranged by size:
1970 697 576
Bsp1286I G_dGCh'C
Cuts at: 0 122 142 155 563 695 989 1180 1202
Size: 122 20 13 408 132 294 191 22
Cuts at: 1202 1337 1696 2047 2785 3243
Size: 135 359 351 738 458
Fragments arranged by size:
738 458 408 359 351 294 191 135
132 122 22 20 13
BspEI T'CCGG_A
Cuts at: 0 3201 3243
Size: 3201 42
BspHI T'CATG_A
Cuts at: 0 2183 3243
Size: 2183 1060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 419 522 1383 3243
Size: 419 103 861 1860
Fragments arranged by size:
1860 861 419 103
BsrBI CCG'CTC
Cuts at: 0 2793 3243
Size: 2793 450
BsrFI r'CCGG_y
Cuts at: 0 590 2058 3243
Size: 590 1468 1185
Fragments arranged by size:
1468 1185 590
BsrGI T'GTAC_A
Cuts at: 0 362 3243
Size: 362 2881
BssHII G'CGCG_C
Cuts at: 0 30 3243
Size: 30 3213
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3134 3243
Size: 3134 109
BstBI TT'CG_AA
Cuts at: 0 2871 3243
Size: 2871 372
BstEII G'GTnAC_C
Cuts at: 0 1239 3243
Size: 1239 2004
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1108 1965 3243
Size: 1108 857 1278
Fragments arranged by size:
1278 1108 857
BstYI r'GATC_y
Cuts at: 0 1151 3243
Size: 1151 2092
BtgI C'CryG_G
Cuts at: 0 512 1826 2020 2459 3243
Size: 512 1314 194 439 784
Fragments arranged by size:
1314 784 512 439 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1994 3243
Size: 1994 1249
BtsI GCAGTG_nn'
Cuts at: 0 1202 3243
Size: 1202 2041
EaeI y'GGCC_r
Cuts at: 0 125 188 840 1098 1224 2117 2288 2849
Size: 125 63 652 258 126 893 171 561
Cuts at: 2849 2913 3243
Size: 64 330
Fragments arranged by size:
893 652 561 330 258 171 126 125
64 63
EagI C'GGCC_G
Cuts at: 0 188 3243
Size: 188 3055
EarI CTCTTCn'nnn_
Cuts at: 0 47 281 574 1475 1506 1532 1699 2380
Size: 47 234 293 901 31 26 167 681
Cuts at: 2380 2662 3243
Size: 282 581
Fragments arranged by size:
901 681 581 293 282 234 167 47
31 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2287 3243
Size: 2287 956
EcoICRI GAG'CTC
Cuts at: 0 153 987 1178 1200 3243
Size: 153 834 191 22 2043
Fragments arranged by size:
2043 834 191 153 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 555 844 934 997 1003 1039 1177
Size: 364 191 289 90 63 6 36 138
Cuts at: 1177 2572 2656 2701 2790 3243
Size: 1395 84 45 89 453
Fragments arranged by size:
1395 453 364 289 191 138 90 89
84 63 45 36 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2200 3243
Size: 2200 1043
EcoO109I rG'GnC_Cy
Cuts at: 0 161 3243
Size: 161 3082
EcoRV GAT'ATC
Cuts at: 0 1563 3243
Size: 1563 1680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1833 3229 3243
Size: 1833 1396 14
Fragments arranged by size:
1833 1396 14
HaeII r_GCGC'y
Cuts at: 0 25 512 662 759 2171 3243
Size: 25 487 150 97 1412 1072
Fragments arranged by size:
1412 1072 487 150 97 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 2929 2961 3030 3062 3105 3137
Size: 208 32 2689 32 69 32 43 32
Cuts at: 3137 3243
Size: 106
Fragments arranged by size:
2689 208 106 69 43 32 32 32
32
HincII GTy'rAC
Cuts at: 0 686 1422 2157 2352 2654 3243
Size: 686 736 735 195 302 589
Fragments arranged by size:
736 735 686 589 302 195
HindIII A'AGCT_T
Cuts at: 0 968 1900 3243
Size: 968 932 1343
Fragments arranged by size:
1343 968 932
HpaI GTT'AAC
Cuts at: 0 2157 3243
Size: 2157 1086
KasI G'GCGC_C
Cuts at: 0 21 3243
Size: 21 3222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 404 419 1091 3243
Size: 314 90 15 672 2152
Fragments arranged by size:
2152 672 314 90 15
MscI TGG'CCA
Cuts at: 0 842 1226 2290 2851 2915 3243
Size: 842 384 1064 561 64 328
Fragments arranged by size:
1064 842 561 384 328 64
MslI CAynn'nnrTG
Cuts at: 0 233 328 676 1106 1963 2242 3243
Size: 233 95 348 430 857 279 1001
Fragments arranged by size:
1001 857 430 348 279 233 95
MspA1I CmG'CkG
Cuts at: 0 476 485 585 644 2022 2284 3243
Size: 476 9 100 59 1378 262 959
Fragments arranged by size:
1378 959 476 262 100 59 9
NarI GG'CG_CC
Cuts at: 0 22 3243
Size: 22 3221
NcoI C'CATG_G
Cuts at: 0 512 1826 3243
Size: 512 1314 1417
Fragments arranged by size:
1417 1314 512
NdeI CA'TA_TG
Cuts at: 0 878 3243
Size: 878 2365
NsiI A_TGCA'T
Cuts at: 0 1125 2801 3243
Size: 1125 1676 442
Fragments arranged by size:
1676 1125 442
NspI r_CATG'y
Cuts at: 0 138 552 834 2247 3243
Size: 138 414 282 1413 996
Fragments arranged by size:
1413 996 414 282 138
PasI CC'CwG_GG
Cuts at: 0 1689 3243
Size: 1689 1554
PciI A'CATG_T
Cuts at: 0 134 3243
Size: 134 3109
PflMI CCAn_nnn'nTGG
Cuts at: 0 213 758 1215 1770 2847 3243
Size: 213 545 457 555 1077 396
Fragments arranged by size:
1077 555 545 457 396 213
PfoI T'CCnGG_A
Cuts at: 0 554 3243
Size: 554 2689
PshAI GACnn'nnGTC
Cuts at: 0 1285 1771 3243
Size: 1285 486 1472
Fragments arranged by size:
1472 1285 486
PstI C_TGCA'G
Cuts at: 0 530 658 863 1196 2177 2309 3243
Size: 530 128 205 333 981 132 934
Fragments arranged by size:
981 934 530 333 205 132 128
PvuII CAG'CTG
Cuts at: 0 476 585 3243
Size: 476 109 2658
Fragments arranged by size:
2658 476 109
SacI G_AGCT'C
Cuts at: 0 155 989 1180 1202 3243
Size: 155 834 191 22 2041
Fragments arranged by size:
2041 834 191 155 22
SacII CC_GC'GG
Cuts at: 0 2023 3243
Size: 2023 1220
SalI G'TCGA_C
Cuts at: 0 1420 2652 3243
Size: 1420 1232 591
Fragments arranged by size:
1420 1232 591
SbfI CC_TGCA'GG
Cuts at: 0 863 1196 3243
Size: 863 333 2047
Fragments arranged by size:
2047 863 333
SfcI C'TryA_G
Cuts at: 0 526 654 859 1192 2173 2305 3243
Size: 526 128 205 333 981 132 938
Fragments arranged by size:
981 938 526 333 205 132 128
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2287 3243
Size: 2287 956
SfoI GGC'GCC
Cuts at: 0 23 3243
Size: 23 3220
SphI G_CATG'C
Cuts at: 0 552 2247 3243
Size: 552 1695 996
Fragments arranged by size:
1695 996 552
StyI C'CwwG_G
Cuts at: 0 296 512 1547 1574 1826 2405 3243
Size: 296 216 1035 27 252 579 838
Fragments arranged by size:
1035 838 579 296 252 216 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 263 751 1797 2018 2442 3243
Size: 263 488 1046 221 424 801
Fragments arranged by size:
1046 801 488 424 263 221
TatI w'GTAC_w
Cuts at: 0 131 362 833 2762 3243
Size: 131 231 471 1929 481
Fragments arranged by size:
1929 481 471 231 131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 406 438 3243
Size: 406 32 2805
Fragments arranged by size:
2805 406 32
Tth111I GACn'n_nGTC
Cuts at: 0 330 3243
Size: 330 2913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 249 1833 3243
Size: 249 1584 1410
Fragments arranged by size:
1584 1410 249
ZraI GAC'GTC
Cuts at: 0 1796 3089 3243
Size: 1796 1293 154
Fragments arranged by size:
1796 1293 154
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlwNI ApaLI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI
MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI
NspI PasI PciI PflMI PfoI PshAI PstI PvuII
SacI SacII SalI SbfI SfcI SfiI SfoI SphI
StyI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AlfI AloI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BciVI BglII
BmtI BpuEI BsiWI BsrDI BssSI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EcoRI FseI FspI FspAI
KpnI MfeI MluI NaeI NgoMIV NheI NotI NruI
PacI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SanDI SapI ScaI SexAI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI
SwaI XbaI XhoI XmaI XmnI