Restriction map for
pFN21AE3580
(Linear) (Six-base) MAPSORT of: KIEE3580.seq Check: 8338 from: 1 to: 2997
KIEE3580.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 150 1494 2869 2997
Size: 150 1344 1375 128
Fragments arranged by size:
1375 1344 150 128
AatII G_ACGT'C
Cuts at: 0 1802 2997
Size: 1802 1195
Acc65I G'GTAC_C
Cuts at: 0 748 2997
Size: 748 2249
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1275 2473 2997
Size: 1275 1198 524
Fragments arranged by size:
1275 1198 524
AfeI AGC'GCT
Cuts at: 0 2506 2997
Size: 2506 491
AleI CACnn'nnGTG
Cuts at: 0 238 757 1675 1891 2997
Size: 238 519 918 216 1106
Fragments arranged by size:
1106 918 519 238 216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1562 1750 2069 2767 2997
Size: 1562 188 319 698 230
Fragments arranged by size:
1562 698 319 230 188
AlwNI CAG_nnn'CTG
Cuts at: 0 1273 2274 2325 2948 2997
Size: 1273 1001 51 623 49
Fragments arranged by size:
1273 1001 623 51 49
ApaI G_GGCC'C
Cuts at: 0 1407 1779 2315 2548 2612 2997
Size: 1407 372 536 233 64 385
Fragments arranged by size:
1407 536 385 372 233 64
AvaI C'yCGr_G
Cuts at: 0 113 579 1087 1321 1358 1428 1469 1663
Size: 113 466 508 234 37 70 41 194
Cuts at: 1663 1994 2343 2541 2560 2611 2997
Size: 331 349 198 19 51 386
Fragments arranged by size:
508 466 386 349 331 234 198 194
113 70 51 41 37 19
BamHI G'GATC_C
Cuts at: 0 584 2997
Size: 584 2413
BanI G'GyrC_C
Cuts at: 0 386 748 1012 1493 1694 1893 2181 2410
Size: 386 362 264 481 201 199 288 229
Cuts at: 2410 2596 2726 2997
Size: 186 130 271
Fragments arranged by size:
481 386 362 288 271 264 229 201
199 186 130
BanII G_rGCy'C
Cuts at: 0 509 649 1407 1476 1779 2315 2449 2548
Size: 509 140 758 69 303 536 134 99
Cuts at: 2548 2612 2997
Size: 64 385
Fragments arranged by size:
758 536 509 385 303 140 134 99
69 64
BbeI G_GCGC'C
Cuts at: 0 1497 2414 2600 2997
Size: 1497 917 186 397
Fragments arranged by size:
1497 917 397 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 270 304 603 637 1312 1346 2997
Size: 270 34 299 34 675 34 1651
Fragments arranged by size:
1651 675 299 270 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 88 257 971 1849 2354 2762 2997
Size: 88 169 714 878 505 408 235
Fragments arranged by size:
878 714 505 408 235 169 88
BglII A'GATC_T
Cuts at: 0 2987 2997
Size: 2987 10
BlpI GC'TnA_GC
Cuts at: 0 2941 2997
Size: 2941 56
Bme1580I G_kGCm'C
Cuts at: 0 235 310 1015 1407 1779 1858 2315 2548
Size: 235 75 705 392 372 79 457 233
Cuts at: 2548 2612 2997
Size: 64 385
Fragments arranged by size:
705 457 392 385 372 235 233 79
75 64
BmgBI CAC'GTC
Cuts at: 0 737 1044 1128 1344 2112 2418 2997
Size: 737 307 84 216 768 306 579
Fragments arranged by size:
768 737 579 307 306 216 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 645 2997
Size: 645 2352
BmtI G_CTAG'C
Cuts at: 0 1975 2997
Size: 1975 1022
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 663 2997
Size: 663 2334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1783 1880 2425 2997
Size: 1783 97 545 572
Fragments arranged by size:
1783 572 545 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 778 1942 2464 2997
Size: 58 720 1164 522 533
Fragments arranged by size:
1164 720 533 522 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 526 2997
Size: 526 2471
BsaAI yAC'GTr
Cuts at: 0 1740 2172 2250 2592 2997
Size: 1740 432 78 342 405
Fragments arranged by size:
1740 432 405 342 78
BsaBI GATnn'nnATC
Cuts at: 0 2089 2997
Size: 2089 908
BsaHI Gr'CG_yC
Cuts at: 0 380 1494 1706 1799 1814 2015 2411 2597
Size: 380 1114 212 93 15 201 396 186
Cuts at: 2597 2997
Size: 400
Fragments arranged by size:
1114 400 396 380 212 201 186 93
15
BsaWI w'CCGG_w
Cuts at: 0 2126 2997
Size: 2126 871
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 602 632 1936 1966 2997
Size: 602 30 1304 30 1031
Fragments arranged by size:
1304 1031 602 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1339 2242 2707 2833 2997
Size: 1339 903 465 126 164
Fragments arranged by size:
1339 903 465 164 126
BseYI C'CCAG_C
Cuts at: 0 13 698 1174 1455 1690 2200 2501 2997
Size: 13 685 476 281 235 510 301 496
Fragments arranged by size:
685 510 496 476 301 281 235 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 313 459 1072 1621 1720 1768 1819
Size: 178 135 146 613 549 99 48 51
Cuts at: 1819 1927 2226 2230 2572 2997
Size: 108 299 4 342 425
Fragments arranged by size:
613 549 425 342 299 178 146 135
108 99 51 48 4
BsiEI CG_ry'CG
Cuts at: 0 103 279 525 2387 2442 2979 2997
Size: 103 176 246 1862 55 537 18
Fragments arranged by size:
1862 537 246 176 103 55 18
BsiHKAI G_wGCw'C
Cuts at: 0 509 1427 1682 1729 2480 2638 2997
Size: 509 918 255 47 751 158 359
Fragments arranged by size:
918 751 509 359 255 158 47
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1060 1241 2997
Size: 1060 181 1756
Fragments arranged by size:
1756 1060 181
Bsp1286I G_dGCh'C
Cuts at: 0 235 310 509 649 1015 1407 1427 1476
Size: 235 75 199 140 366 392 20 49
Cuts at: 1476 1682 1729 1779 1858 2315 2449 2480 2548
Size: 206 47 50 79 457 134 31 68
Cuts at: 2548 2612 2638 2997
Size: 64 26 359
Fragments arranged by size:
457 392 366 359 235 206 199 140
134 79 75 68 64 50 49 47
31 26 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 150 359 1265 1494 1616 1814 2594 2869
Size: 150 209 906 229 122 198 780 275
Cuts at: 2869 2997
Size: 128
Fragments arranged by size:
906 780 275 229 209 198 150 128
122
BsrBI CCG'CTC
Cuts at: 0 538 1534 2517 2997
Size: 538 996 983 480
Fragments arranged by size:
996 983 538 480
BsrFI r'CCGG_y
Cuts at: 0 328 858 1316 1864 2837 2997
Size: 328 530 458 548 973 160
Fragments arranged by size:
973 548 530 458 328 160
BssHII G'CGCG_C
Cuts at: 0 341 1353 2699 2701 2997
Size: 341 1012 1346 2 296
Fragments arranged by size:
1346 1012 341 296 2
BssSI C'ACGA_G
Cuts at: 0 1063 2893 2997
Size: 1063 1830 104
Fragments arranged by size:
1830 1063 104
BstAPI GCAn_nnn'nTGC
Cuts at: 0 351 426 482 2726 2861 2997
Size: 351 75 56 2244 135 136
Fragments arranged by size:
2244 351 136 135 75 56
BstYI r'GATC_y
Cuts at: 0 584 2084 2987 2997
Size: 584 1500 903 10
Fragments arranged by size:
1500 903 584 10
BtgI C'CryG_G
Cuts at: 0 255 693 966 1035 2436 2910 2997
Size: 255 438 273 69 1401 474 87
Fragments arranged by size:
1401 474 438 273 255 87 69
BtsI GCAGTG_nn'
Cuts at: 0 301 487 1633 1732 2214 2997
Size: 301 186 1146 99 482 783
Fragments arranged by size:
1146 783 482 301 186 99
ClaI AT'CG_AT
Cuts at: 0 1904 2997
Size: 1904 1093
DraIII CAC_nnn'GTG
Cuts at: 0 482 2687 2997
Size: 482 2205 310
Fragments arranged by size:
2205 482 310
DrdI GACnn_nn'nnGTC
Cuts at: 0 940 2997
Size: 940 2057
EaeI y'GGCC_r
Cuts at: 0 100 249 725 1346 1742 2459 2907 2997
Size: 100 149 476 621 396 717 448 90
Fragments arranged by size:
717 621 476 448 396 149 100 90
EagI C'GGCC_G
Cuts at: 0 100 2997
Size: 100 2897
EarI CTCTTCn'nnn_
Cuts at: 0 1405 2997
Size: 1405 1592
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 877 1006 1717 1830 2878 2997
Size: 877 129 711 113 1048 119
Fragments arranged by size:
1048 877 711 129 119 113
EcoICRI GAG'CTC
Cuts at: 0 507 2997
Size: 507 2490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1275 1783 1880 2425 2473 2997
Size: 1275 508 97 545 48 524
Fragments arranged by size:
1275 545 524 508 97 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1558 1888 2997
Size: 1558 330 1109
Fragments arranged by size:
1558 1109 330
EcoO109I rG'GnC_Cy
Cuts at: 0 110 573 628 1776 1881 2304 2311 2312
Size: 110 463 55 1148 105 423 7 1
Cuts at: 2312 2484 2545 2564 2608 2997
Size: 172 61 19 44 389
Fragments arranged by size:
1148 463 423 389 172 110 105 61
55 44 19 7 1
FspI TGC'GCA
Cuts at: 0 2122 2997
Size: 2122 875
HaeII r_GCGC'y
Cuts at: 0 1096 1456 1497 2044 2257 2404 2414 2508
Size: 1096 360 41 547 213 147 10 94
Cuts at: 2508 2600 2650 2783 2948 2997
Size: 92 50 133 165 49
Fragments arranged by size:
1096 547 360 213 165 147 133 94
92 50 49 41 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1804 1836 1977 2009 2997
Size: 1804 32 141 32 988
Fragments arranged by size:
1804 988 141 32 32
HincII GTy'rAC
Cuts at: 0 1803 2214 2997
Size: 1803 411 783
Fragments arranged by size:
1803 783 411
HindIII A'AGCT_T
Cuts at: 0 1396 2997
Size: 1396 1601
KasI G'GCGC_C
Cuts at: 0 1493 2410 2596 2997
Size: 1493 917 186 401
Fragments arranged by size:
1493 917 401 186
KpnI G_GTAC'C
Cuts at: 0 752 2997
Size: 752 2245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2422 2997
Size: 2422 575
MscI TGG'CCA
Cuts at: 0 727 2997
Size: 727 2270
MslI CAynn'nnrTG
Cuts at: 0 172 238 757 1675 1891 2997
Size: 172 66 519 918 216 1106
Fragments arranged by size:
1106 918 519 216 172 66
MspA1I CmG'CkG
Cuts at: 0 814 1037 1281 2007 2105 2145 2271 2322
Size: 814 223 244 726 98 40 126 51
Cuts at: 2322 2438 2451 2620 2811 2997
Size: 116 13 169 191 186
Fragments arranged by size:
814 726 244 223 191 186 169 126
116 98 51 40 13
NaeI GCC'GGC
Cuts at: 0 330 860 1866 2997
Size: 330 530 1006 1131
Fragments arranged by size:
1131 1006 530 330
NarI GG'CG_CC
Cuts at: 0 1494 2411 2597 2997
Size: 1494 917 186 400
Fragments arranged by size:
1494 917 400 186
NgoMIV G'CCGG_C
Cuts at: 0 328 858 1864 2997
Size: 328 530 1006 1133
Fragments arranged by size:
1133 1006 530 328
NheI G'CTAG_C
Cuts at: 0 1971 2997
Size: 1971 1026
NspI r_CATG'y
Cuts at: 0 171 492 2921 2997
Size: 171 321 2429 76
Fragments arranged by size:
2429 321 171 76
PasI CC'CwG_GG
Cuts at: 0 1212 1650 2308 2997
Size: 1212 438 658 689
Fragments arranged by size:
1212 689 658 438
PflMI CCAn_nnn'nTGG
Cuts at: 0 2814 2997
Size: 2814 183
PfoI T'CCnGG_A
Cuts at: 0 1760 2093 2510 2997
Size: 1760 333 417 487
Fragments arranged by size:
1760 487 417 333
PmlI CAC'GTG
Cuts at: 0 1740 2172 2250 2592 2997
Size: 1740 432 78 342 405
Fragments arranged by size:
1740 432 405 342 78
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1357 1389 2997
Size: 1357 32 1608
Fragments arranged by size:
1608 1357 32
PpuMI rG'GwC_Cy
Cuts at: 0 110 1881 2304 2564 2997
Size: 110 1771 423 260 433
Fragments arranged by size:
1771 433 423 260 110
PspOMI G'GGCC_C
Cuts at: 0 1403 1775 2311 2544 2608 2997
Size: 1403 372 536 233 64 389
Fragments arranged by size:
1403 536 389 372 233 64
PstI C_TGCA'G
Cuts at: 0 62 872 1172 2138 2261 2828 2997
Size: 62 810 300 966 123 567 169
Fragments arranged by size:
966 810 567 300 169 123 62
PvuII CAG'CTG
Cuts at: 0 2007 2271 2322 2811 2997
Size: 2007 264 51 489 186
Fragments arranged by size:
2007 489 264 186 51
RsrII CG'GwC_CG
Cuts at: 0 1032 2997
Size: 1032 1965
SacI G_AGCT'C
Cuts at: 0 509 2997
Size: 509 2488
SacII CC_GC'GG
Cuts at: 0 1038 2439 2997
Size: 1038 1401 558
Fragments arranged by size:
1401 1038 558
SanDI GG'GwC_CC
Cuts at: 0 2304 2564 2997
Size: 2304 260 433
Fragments arranged by size:
2304 433 260
SfcI C'TryA_G
Cuts at: 0 58 868 1168 2134 2257 2335 2824 2997
Size: 58 810 300 966 123 78 489 173
Fragments arranged by size:
966 810 489 300 173 123 78 58
SfoI GGC'GCC
Cuts at: 0 1495 2412 2598 2997
Size: 1495 917 186 399
Fragments arranged by size:
1495 917 399 186
SmaI CCC'GGG
Cuts at: 0 1089 1360 1430 1471 2543 2997
Size: 1089 271 70 41 1072 454
Fragments arranged by size:
1089 1072 454 271 70 41
SmlI C'TyrA_G
Cuts at: 0 73 793 1957 2479 2997
Size: 73 720 1164 522 518
Fragments arranged by size:
1164 720 522 518 73
SphI G_CATG'C
Cuts at: 0 171 2921 2997
Size: 171 2750 76
Fragments arranged by size:
2750 171 76
SrfI GCCC'GGGC
Cuts at: 0 1471 2997
Size: 1471 1526
StuI AGG'CCT
Cuts at: 0 2341 2997
Size: 2341 656
StyI C'CwwG_G
Cuts at: 0 844 1521 2997
Size: 844 677 1476
Fragments arranged by size:
1476 844 677
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1107 1682 1735 2192 2997
Size: 1107 575 53 457 805
Fragments arranged by size:
1107 805 575 457 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 181 213 435 467 546 578 602 634
Size: 181 32 222 32 79 32 24 32
Cuts at: 634 781 813 1245 1277 1424 1456 1936 1968
Size: 147 32 432 32 147 32 480 32
Cuts at: 1968 2188 2220 2997
Size: 220 32 777
Fragments arranged by size:
777 480 432 222 220 181 147 147
79 32 32 32 32 32 32 32
32 32 24
Tth111I GACn'n_nGTC
Cuts at: 0 916 1129 2997
Size: 916 213 1868
Fragments arranged by size:
1868 916 213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1341 1599 2997
Size: 1341 258 1398
Fragments arranged by size:
1398 1341 258
XmaI C'CCGG_G
Cuts at: 0 1087 1358 1428 1469 2541 2997
Size: 1087 271 70 41 1072 456
Fragments arranged by size:
1087 1072 456 271 70 41
ZraI GAC'GTC
Cuts at: 0 1800 2997
Size: 1800 1197
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AleI AlfI AlwNI
ApaI AvaI BamHI BanI BanII BbeI BcgI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstYI BtgI BtsI ClaI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuII RsrII SacI SacII SanDI SfcI
SfoI SmaI SmlI SphI SrfI StuI StyI TaqII
TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AgeI AhdI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI
BciVI BclI BfrBI Bpu10I BsiWI BsmI BspEI BspHI
BsrDI BsrGI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
CspCI DraI EcoRI EcoRV FalI FseI FspAI HpaI
MfeI MluI NcoI NdeI NotI NruI NsiI PacI
PciI PmeI PshAI PsiI PspXI PsrI PvuI SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SspI SwaI TatI XbaI XhoI XmnI