Restriction map for
pFN21AE3562
(Linear) (Six-base) MAPSORT of: KIEE3562.seq Check: 8501 from: 1 to: 1350
KIEE3562.orf
With 182 enzymes: SgfI *
July 3, 2012 18:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 49 1350
Size: 49 1301
AccI GT'mk_AC
Cuts at: 0 669 979 1350
Size: 669 310 371
Fragments arranged by size:
669 371 310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 837 1350
Size: 718 119 513
Fragments arranged by size:
718 513 119
AflIII A'CryG_T
Cuts at: 0 950 1350
Size: 950 400
AleI CACnn'nnGTG
Cuts at: 0 829 1350
Size: 829 521
AlwNI CAG_nnn'CTG
Cuts at: 0 73 1350
Size: 73 1277
ApoI r'AATT_y
Cuts at: 0 516 1350
Size: 516 834
BanII G_rGCy'C
Cuts at: 0 38 999 1350
Size: 38 961 351
Fragments arranged by size:
961 351 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 917 1350
Size: 917 433
BbvCI CC'TCA_GC
Cuts at: 0 788 1006 1350
Size: 788 218 344
Fragments arranged by size:
788 344 218
BclI T'GATC_A
Cuts at: 0 533 1350
Size: 533 817
Bme1580I G_kGCm'C
Cuts at: 0 95 1350
Size: 95 1255
BmrI ACTGGGnnnn_n'
Cuts at: 0 836 1035 1350
Size: 836 199 315
Fragments arranged by size:
836 315 199
Bpu10I CC'TnA_GC
Cuts at: 0 788 1006 1350
Size: 788 218 344
Fragments arranged by size:
788 344 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1350
Size: 619 731
BsaI GGTCTCn'nnnn_
Cuts at: 0 475 1350
Size: 475 875
BsaAI yAC'GTr
Cuts at: 0 591 1350
Size: 591 759
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 126 156 1350
Size: 126 30 1194
Fragments arranged by size:
1194 126 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 183 951 1350
Size: 36 147 768 399
Fragments arranged by size:
768 399 147 36
BsiHKAI G_wGCw'C
Cuts at: 0 38 999 1350
Size: 38 961 351
Fragments arranged by size:
961 351 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 740 1350
Size: 740 610
Bsp1286I G_dGCh'C
Cuts at: 0 38 95 999 1350
Size: 38 57 904 351
Fragments arranged by size:
904 351 57 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 737 1350
Size: 49 688 613
Fragments arranged by size:
688 613 49
BstBI TT'CG_AA
Cuts at: 0 908 1350
Size: 908 442
BstYI r'GATC_y
Cuts at: 0 1001 1342 1350
Size: 1001 341 8
Fragments arranged by size:
1001 341 8
BstZ17I GTA'TAC
Cuts at: 0 670 980 1350
Size: 670 310 370
Fragments arranged by size:
670 370 310
Bsu36I CC'TnA_GG
Cuts at: 0 551 734 916 1350
Size: 551 183 182 434
Fragments arranged by size:
551 434 183 182
BtgI C'CryG_G
Cuts at: 0 1338 1350
Size: 1338 12
BtsI GCAGTG_nn'
Cuts at: 0 25 934 1350
Size: 25 909 416
Fragments arranged by size:
909 416 25
DraIII CAC_nnn'GTG
Cuts at: 0 20 1350
Size: 20 1330
EaeI y'GGCC_r
Cuts at: 0 779 1350
Size: 779 571
EarI CTCTTCn'nnn_
Cuts at: 0 572 1171 1350
Size: 572 599 179
Fragments arranged by size:
599 572 179
EcoICRI GAG'CTC
Cuts at: 0 36 997 1350
Size: 36 961 353
Fragments arranged by size:
961 353 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 837 1350
Size: 718 119 513
Fragments arranged by size:
718 513 119
EcoNI CCTnn'n_nnAGG
Cuts at: 0 733 1350
Size: 733 617
EcoO109I rG'GnC_Cy
Cuts at: 0 1205 1350
Size: 1205 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 224 256 848 880 986 1018 1350
Size: 224 32 592 32 106 32 332
Fragments arranged by size:
592 332 224 106 32 32 32
HindIII A'AGCT_T
Cuts at: 0 898 1350
Size: 898 452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1350
Size: 988 362
MscI TGG'CCA
Cuts at: 0 781 1350
Size: 781 569
MslI CAynn'nnrTG
Cuts at: 0 829 1350
Size: 829 521
NspI r_CATG'y
Cuts at: 0 364 954 1104 1350
Size: 364 590 150 246
Fragments arranged by size:
590 364 246 150
PciI A'CATG_T
Cuts at: 0 950 1350
Size: 950 400
PfoI T'CCnGG_A
Cuts at: 0 1229 1350
Size: 1229 121
PsiI TTA'TAA
Cuts at: 0 514 1350
Size: 514 836
SacI G_AGCT'C
Cuts at: 0 38 999 1350
Size: 38 961 351
Fragments arranged by size:
961 351 38
SapI GCTCTTCn'nnn_
Cuts at: 0 572 1350
Size: 572 778
SfcI C'TryA_G
Cuts at: 0 276 405 1350
Size: 276 129 945
Fragments arranged by size:
945 276 129
SmlI C'TyrA_G
Cuts at: 0 634 1350
Size: 634 716
SphI G_CATG'C
Cuts at: 0 364 1350
Size: 364 986
StyI C'CwwG_G
Cuts at: 0 355 869 1201 1209 1350
Size: 355 514 332 8 141
Fragments arranged by size:
514 355 332 141 8
TatI w'GTAC_w
Cuts at: 0 185 970 1350
Size: 185 785 380
Fragments arranged by size:
785 380 185
Tth111I GACn'n_nGTC
Cuts at: 0 73 1350
Size: 73 1277
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApoI BanII
BbsI BbvCI BclI Bme1580I BmrI Bpu10I BpuEI BsaI
BsaAI BsaXI BsgI BsiHKAI BsmBI Bsp1286I BspMI BstBI
BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I Hin4I HindIII MmeI MscI
MslI NspI PciI PfoI PsiI SacI SapI SfcI
SmlI SphI StyI TatI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpmI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TstI XbaI XcmI XhoI XmaI XmnI ZraI