Restriction map for
pFN21AE3540
(Linear) (Six-base) MAPSORT of: KIEE3540.seq Check: 9869 from: 1 to: 906
KIEE3540.orf
With 182 enzymes: SgfI *
July 3, 2012 18:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 96 798 906
Size: 96 702 108
Fragments arranged by size:
702 108 96
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 906
Size: 847 59
AflIII A'CryG_T
Cuts at: 0 453 906
Size: 453 453
AlwNI CAG_nnn'CTG
Cuts at: 0 96 633 678 795 906
Size: 96 537 45 117 111
Fragments arranged by size:
537 117 111 96 45
ApaI G_GGCC'C
Cuts at: 0 538 906
Size: 538 368
ApaLI G'TGCA_C
Cuts at: 0 210 847 906
Size: 210 637 59
Fragments arranged by size:
637 210 59
AvaI C'yCGr_G
Cuts at: 0 762 871 906
Size: 762 109 35
Fragments arranged by size:
762 109 35
BanII G_rGCy'C
Cuts at: 0 8 538 906
Size: 8 530 368
Fragments arranged by size:
530 368 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 150 586 906
Size: 150 436 320
Fragments arranged by size:
436 320 150
BclI T'GATC_A
Cuts at: 0 293 506 906
Size: 293 213 400
Fragments arranged by size:
400 293 213
BglI GCCn_nnn'nGGC
Cuts at: 0 17 737 886 906
Size: 17 720 149 20
Fragments arranged by size:
720 149 20 17
Bme1580I G_kGCm'C
Cuts at: 0 214 538 851 906
Size: 214 324 313 55
Fragments arranged by size:
324 313 214 55
BmgBI CAC'GTC
Cuts at: 0 504 846 906
Size: 504 342 60
Fragments arranged by size:
504 342 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 906
Size: 574 332
BsaI GGTCTCn'nnnn_
Cuts at: 0 61 906
Size: 61 845
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 823 853 906
Size: 214 30 579 30 53
Fragments arranged by size:
579 214 53 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 510 906
Size: 510 396
BseYI C'CCAG_C
Cuts at: 0 724 906
Size: 724 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 690 771 808 906
Size: 619 71 81 37 98
Fragments arranged by size:
619 98 81 71 37
BsiHKAI G_wGCw'C
Cuts at: 0 214 769 851 906
Size: 214 555 82 55
Fragments arranged by size:
555 214 82 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 215 906
Size: 215 691
Bsp1286I G_dGCh'C
Cuts at: 0 8 214 538 769 851 906
Size: 8 206 324 231 82 55
Fragments arranged by size:
324 231 206 82 55 8
BspHI T'CATG_A
Cuts at: 0 476 575 906
Size: 476 99 331
Fragments arranged by size:
476 331 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 79 96 263 798 906
Size: 79 17 167 535 108
Fragments arranged by size:
535 167 108 79 17
BsrFI r'CCGG_y
Cuts at: 0 128 783 906
Size: 128 655 123
Fragments arranged by size:
655 128 123
BtgI C'CryG_G
Cuts at: 0 347 356 530 686 906
Size: 347 9 174 156 220
Fragments arranged by size:
347 220 174 156 9
DrdI GACnn_nn'nnGTC
Cuts at: 0 154 382 906
Size: 154 228 524
Fragments arranged by size:
524 228 154
EaeI y'GGCC_r
Cuts at: 0 87 419 729 906
Size: 87 332 310 177
Fragments arranged by size:
332 310 177 87
EarI CTCTTCn'nnn_
Cuts at: 0 556 906
Size: 556 350
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 320 906
Size: 320 586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 906
Size: 847 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 649 906
Size: 325 324 257
Fragments arranged by size:
325 324 257
EcoO109I rG'GnC_Cy
Cuts at: 0 93 535 888 906
Size: 93 442 353 18
Fragments arranged by size:
442 353 93 18
HaeII r_GCGC'y
Cuts at: 0 135 906
Size: 135 771
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 906
Size: 214 32 660
Fragments arranged by size:
660 214 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 866 906
Size: 173 693 40
Fragments arranged by size:
693 173 40
MscI TGG'CCA
Cuts at: 0 421 906
Size: 421 485
MslI CAynn'nnrTG
Cuts at: 0 458 906
Size: 458 448
MspA1I CmG'CkG
Cuts at: 0 117 728 795 825 906
Size: 117 611 67 30 81
Fragments arranged by size:
611 117 81 67 30
NcoI C'CATG_G
Cuts at: 0 530 906
Size: 530 376
NspI r_CATG'y
Cuts at: 0 457 906
Size: 457 449
PciI A'CATG_T
Cuts at: 0 453 906
Size: 453 453
PfoI T'CCnGG_A
Cuts at: 0 776 906
Size: 776 130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 906
Size: 43 32 831
Fragments arranged by size:
831 43 32
PspOMI G'GGCC_C
Cuts at: 0 534 906
Size: 534 372
PstI C_TGCA'G
Cuts at: 0 76 107 268 906
Size: 76 31 161 638
Fragments arranged by size:
638 161 76 31
PvuII CAG'CTG
Cuts at: 0 117 825 906
Size: 117 708 81
Fragments arranged by size:
708 117 81
ScaI AGT'ACT
Cuts at: 0 832 906
Size: 832 74
SfcI C'TryA_G
Cuts at: 0 72 103 264 906
Size: 72 31 161 642
Fragments arranged by size:
642 161 72 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 17 906
Size: 17 889
SgrAI Cr'CCGG_yG
Cuts at: 0 128 906
Size: 128 778
SmlI C'TyrA_G
Cuts at: 0 589 906
Size: 589 317
StyI C'CwwG_G
Cuts at: 0 530 906
Size: 530 376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 448 906
Size: 448 458
TatI w'GTAC_w
Cuts at: 0 830 906
Size: 830 76
Tth111I GACn'n_nGTC
Cuts at: 0 199 906
Size: 199 707
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApaLI AvaI BanII
BbsI BclI BglI Bme1580I BmgBI BpuEI BsaI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrFI BtgI DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NcoI
NspI PciI PfoI PpiI PspOMI PstI PvuII ScaI
SfcI SfiI SgrAI SmlI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglII BlpI BmrI BmtI BplI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfoI SgfI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI XbaI XcmI
XhoI XmaI XmnI ZraI