Restriction map for
pFN21AE3528
(Linear) (Six-base) MAPSORT of: KIEE3528.seq Check: 9810 from: 1 to: 2736
KIEE3528.orf
With 182 enzymes: SgfI *
July 3, 2012 18:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 221 2736
Size: 221 2515
AccI GT'mk_AC
Cuts at: 0 2311 2736
Size: 2311 425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 571 2056 2224 2736
Size: 397 174 1485 168 512
Fragments arranged by size:
1485 512 397 174 168
AflIII A'CryG_T
Cuts at: 0 236 465 2736
Size: 236 229 2271
Fragments arranged by size:
2271 236 229
AleI CACnn'nnGTG
Cuts at: 0 1252 2736
Size: 1252 1484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 388 1130 2736
Size: 388 742 1606
Fragments arranged by size:
1606 742 388
AlwNI CAG_nnn'CTG
Cuts at: 0 846 1942 2736
Size: 846 1096 794
Fragments arranged by size:
1096 846 794
ApaLI G'TGCA_C
Cuts at: 0 1661 2736
Size: 1661 1075
ApoI r'AATT_y
Cuts at: 0 32 136 146 1212 1410 2233 2508 2736
Size: 32 104 10 1066 198 823 275 228
Fragments arranged by size:
1066 823 275 228 198 104 32 10
AvaI C'yCGr_G
Cuts at: 0 670 2736
Size: 670 2066
BamHI G'GATC_C
Cuts at: 0 2451 2736
Size: 2451 285
BanI G'GyrC_C
Cuts at: 0 819 2052 2247 2736
Size: 819 1233 195 489
Fragments arranged by size:
1233 819 489 195
BanII G_rGCy'C
Cuts at: 0 2503 2736
Size: 2503 233
BbsI GAAGACnn'nnnn_
Cuts at: 0 52 1163 2736
Size: 52 1111 1573
Fragments arranged by size:
1573 1111 52
BclI T'GATC_A
Cuts at: 0 2006 2736
Size: 2006 730
BfrBI ATG'CAT
Cuts at: 0 1230 2736
Size: 1230 1506
BglII A'GATC_T
Cuts at: 0 1419 2736
Size: 1419 1317
BlpI GC'TnA_GC
Cuts at: 0 157 2736
Size: 157 2579
Bme1580I G_kGCm'C
Cuts at: 0 1665 2358 2736
Size: 1665 693 378
Fragments arranged by size:
1665 693 378
BmrI ACTGGGnnnn_n'
Cuts at: 0 59 1199 2368 2736
Size: 59 1140 1169 368
Fragments arranged by size:
1169 1140 368 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1494 2185 2240 2443 2736
Size: 1494 691 55 203 293
Fragments arranged by size:
1494 691 293 203 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1321 1671 2165 2736
Size: 929 392 350 494 571
Fragments arranged by size:
929 571 494 392 350
BsaI GGTCTCn'nnnn_
Cuts at: 0 1853 2290 2590 2736
Size: 1853 437 300 146
Fragments arranged by size:
1853 437 300 146
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2230 2680 2736
Size: 2230 450 56
Fragments arranged by size:
2230 450 56
BseYI C'CCAG_C
Cuts at: 0 998 2736
Size: 998 1738
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1357 1862 2736
Size: 1357 505 874
Fragments arranged by size:
1357 874 505
BsiHKAI G_wGCw'C
Cuts at: 0 785 1665 2503 2736
Size: 785 880 838 233
Fragments arranged by size:
880 838 785 233
BsmI GAATG_Cn'
Cuts at: 0 894 1803 2736
Size: 894 909 933
Fragments arranged by size:
933 909 894
Bsp1286I G_dGCh'C
Cuts at: 0 785 1665 2358 2503 2736
Size: 785 880 693 145 233
Fragments arranged by size:
880 785 693 233 145
BspHI T'CATG_A
Cuts at: 0 1119 2736
Size: 1119 1617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 221 1379 2736
Size: 221 1158 1357
Fragments arranged by size:
1357 1158 221
BsrDI GCAATG_nn'
Cuts at: 0 1629 2736
Size: 1629 1107
BsrGI T'GTAC_A
Cuts at: 0 528 2736
Size: 528 2208
BstEII G'GTnAC_C
Cuts at: 0 793 2736
Size: 793 1943
BstXI CCAn_nnnn'nTGG
Cuts at: 0 49 2736
Size: 49 2687
BstYI r'GATC_y
Cuts at: 0 1419 2176 2451 2736
Size: 1419 757 275 285
Fragments arranged by size:
1419 757 285 275
Bsu36I CC'TnA_GG
Cuts at: 0 2726 2736
Size: 2726 10
BtgI C'CryG_G
Cuts at: 0 955 2736
Size: 955 1781
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2543 2736
Size: 2543 193
BtsI GCAGTG_nn'
Cuts at: 0 2212 2736
Size: 2212 524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1379 1414 2736
Size: 1379 35 1322
Fragments arranged by size:
1379 1322 35
DraI TTT'AAA
Cuts at: 0 1431 2736
Size: 1431 1305
EarI CTCTTCn'nnn_
Cuts at: 0 1433 1468 1649 1979 2288 2736
Size: 1433 35 181 330 309 448
Fragments arranged by size:
1433 448 330 309 181 35
EcoICRI GAG'CTC
Cuts at: 0 2501 2736
Size: 2501 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 571 1494 2056 2185 2224 2240 2443
Size: 397 174 923 562 129 39 16 203
Cuts at: 2443 2736
Size: 293
Fragments arranged by size:
923 562 397 293 203 174 129 39
16
EcoO109I rG'GnC_Cy
Cuts at: 0 1515 2736
Size: 1515 1221
EcoRI G'AATT_C
Cuts at: 0 1410 2736
Size: 1410 1326
EcoRV GAT'ATC
Cuts at: 0 5 2736
Size: 5 2731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1459 1628 2736
Size: 1459 169 1108
Fragments arranged by size:
1459 1108 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 2289 2321 2436 2468 2736
Size: 244 32 2013 32 115 32 268
Fragments arranged by size:
2013 268 244 115 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1316 2736
Size: 1316 1420
MslI CAynn'nnrTG
Cuts at: 0 1252 2736
Size: 1252 1484
MspA1I CmG'CkG
Cuts at: 0 156 1002 1149 2736
Size: 156 846 147 1587
Fragments arranged by size:
1587 846 156 147
NcoI C'CATG_G
Cuts at: 0 955 2736
Size: 955 1781
NsiI A_TGCA'T
Cuts at: 0 1232 2736
Size: 1232 1504
NspI r_CATG'y
Cuts at: 0 469 1107 1234 2649 2736
Size: 469 638 127 1415 87
Fragments arranged by size:
1415 638 469 127 87
PciI A'CATG_T
Cuts at: 0 465 2736
Size: 465 2271
PfoI T'CCnGG_A
Cuts at: 0 293 1685 2736
Size: 293 1392 1051
Fragments arranged by size:
1392 1051 293
PpuMI rG'GwC_Cy
Cuts at: 0 1515 2736
Size: 1515 1221
PsiI TTA'TAA
Cuts at: 0 142 2736
Size: 142 2594
PstI C_TGCA'G
Cuts at: 0 851 2736
Size: 851 1885
PvuII CAG'CTG
Cuts at: 0 156 1002 1149 2736
Size: 156 846 147 1587
Fragments arranged by size:
1587 846 156 147
SacI G_AGCT'C
Cuts at: 0 2503 2736
Size: 2503 233
SapI GCTCTTCn'nnn_
Cuts at: 0 1433 2736
Size: 1433 1303
SfcI C'TryA_G
Cuts at: 0 264 847 1023 1539 2312 2466 2736
Size: 264 583 176 516 773 154 270
Fragments arranged by size:
773 583 516 270 264 176 154
SmlI C'TyrA_G
Cuts at: 0 944 1336 1650 2180 2736
Size: 944 392 314 530 556
Fragments arranged by size:
944 556 530 392 314
SspI AAT'ATT
Cuts at: 0 1091 2736
Size: 1091 1645
StuI AGG'CCT
Cuts at: 0 2464 2554 2736
Size: 2464 90 182
Fragments arranged by size:
2464 182 90
StyI C'CwwG_G
Cuts at: 0 955 2349 2420 2736
Size: 955 1394 71 316
Fragments arranged by size:
1394 955 316 71
TatI w'GTAC_w
Cuts at: 0 528 1027 2736
Size: 528 499 1709
Fragments arranged by size:
1709 528 499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1185 1217 2736
Size: 1185 32 1519
Fragments arranged by size:
1519 1185 32
Tth111I GACn'n_nGTC
Cuts at: 0 206 2119 2736
Size: 206 1913 617
Fragments arranged by size:
1913 617 206
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1767 1825 2736
Size: 1767 58 911
Fragments arranged by size:
1767 911 58
XmnI GAAnn'nnTTC
Cuts at: 0 289 965 1018 2237 2736
Size: 289 676 53 1219 499
Fragments arranged by size:
1219 676 499 289 53
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BclI BfrBI
BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI Hin4I HindIII MslI MspA1I NcoI NsiI NspI
PciI PfoI PpuMI PsiI PstI PvuII SacI SapI
SfcI SmlI SspI StuI StyI TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BcgI BciVI BglI BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstZ17I ClaI
DraIII DrdI EaeI EagI EciI EcoNI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PflMI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TaqII XbaI XhoI
XmaI ZraI