Restriction map for
pFN21AE3524
(Linear) (Six-base) MAPSORT of: KIEE3524.seq Check: 7666 from: 1 to: 1710
KIEE3524.orf
With 182 enzymes: SgfI *
July 3, 2012 18:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 455 1026 1710
Size: 455 571 684
Fragments arranged by size:
684 571 455
AccI GT'mk_AC
Cuts at: 0 1081 1710
Size: 1081 629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 739 1003 1344 1710
Size: 169 570 264 341 366
Fragments arranged by size:
570 366 341 264 169
AfeI AGC'GCT
Cuts at: 0 1242 1710
Size: 1242 468
AflIII A'CryG_T
Cuts at: 0 185 590 689 818 1710
Size: 185 405 99 129 892
Fragments arranged by size:
892 405 185 129 99
AhdI GACnn_n'nnGTC
Cuts at: 0 117 1710
Size: 117 1593
AleI CACnn'nnGTG
Cuts at: 0 554 937 1710
Size: 554 383 773
Fragments arranged by size:
773 554 383
AlwNI CAG_nnn'CTG
Cuts at: 0 435 648 777 1710
Size: 435 213 129 933
Fragments arranged by size:
933 435 213 129
ApaI G_GGCC'C
Cuts at: 0 215 1710
Size: 215 1495
ApaLI G'TGCA_C
Cuts at: 0 805 940 1710
Size: 805 135 770
Fragments arranged by size:
805 770 135
BamHI G'GATC_C
Cuts at: 0 1408 1710
Size: 1408 302
BanI G'GyrC_C
Cuts at: 0 1560 1710
Size: 1560 150
BanII G_rGCy'C
Cuts at: 0 215 356 1710
Size: 215 141 1354
Fragments arranged by size:
1354 215 141
BbsI GAAGACnn'nnnn_
Cuts at: 0 156 793 1138 1350 1710
Size: 156 637 345 212 360
Fragments arranged by size:
637 360 345 212 156
BciVI GTATCCnnnnn_n'
Cuts at: 0 529 1710
Size: 529 1181
Bme1580I G_kGCm'C
Cuts at: 0 215 809 944 1710
Size: 215 594 135 766
Fragments arranged by size:
766 594 215 135
BmgBI CAC'GTC
Cuts at: 0 819 1356 1710
Size: 819 537 354
Fragments arranged by size:
819 537 354
BmrI ACTGGGnnnn_n'
Cuts at: 0 650 1710
Size: 650 1060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1086 1238 1239 1493 1508 1710
Size: 1051 35 152 1 254 15 202
Fragments arranged by size:
1051 254 202 152 35 15 1
Bpu10I CC'TnA_GC
Cuts at: 0 1438 1710
Size: 1438 272
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 1710
Size: 310 1400
BsaAI yAC'GTr
Cuts at: 0 804 1710
Size: 804 906
BsaHI Gr'CG_yC
Cuts at: 0 224 1710
Size: 224 1486
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 830 860 1024 1054 1212 1242 1710
Size: 830 30 164 30 158 30 468
Fragments arranged by size:
830 468 164 158 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1241 1710
Size: 1241 469
BseYI C'CCAG_C
Cuts at: 0 237 908 1710
Size: 237 671 802
Fragments arranged by size:
802 671 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 940 1710
Size: 421 519 770
Fragments arranged by size:
770 519 421
BsiEI CG_ry'CG
Cuts at: 0 1622 1710
Size: 1622 88
BsiHKAI G_wGCw'C
Cuts at: 0 356 809 944 1444 1710
Size: 356 453 135 500 266
Fragments arranged by size:
500 453 356 266 135
BsmI GAATG_Cn'
Cuts at: 0 24 1204 1468 1710
Size: 24 1180 264 242
Fragments arranged by size:
1180 264 242 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 176 1511 1710
Size: 176 1335 199
Fragments arranged by size:
1335 199 176
Bsp1286I G_dGCh'C
Cuts at: 0 215 356 809 944 1444 1710
Size: 215 141 453 135 500 266
Fragments arranged by size:
500 453 266 215 141 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 455 535 1026 1710
Size: 455 80 491 684
Fragments arranged by size:
684 491 455 80
BsrBI CCG'CTC
Cuts at: 0 953 1710
Size: 953 757
BsrFI r'CCGG_y
Cuts at: 0 884 1383 1533 1710
Size: 884 499 150 177
Fragments arranged by size:
884 499 177 150
BsrGI T'GTAC_A
Cuts at: 0 680 1292 1710
Size: 680 612 418
Fragments arranged by size:
680 612 418
BssHII G'CGCG_C
Cuts at: 0 156 1710
Size: 156 1554
BssSI C'ACGA_G
Cuts at: 0 138 1591 1710
Size: 138 1453 119
Fragments arranged by size:
1453 138 119
BstAPI GCAn_nnn'nTGC
Cuts at: 0 252 582 1243 1710
Size: 252 330 661 467
Fragments arranged by size:
661 467 330 252
BstBI TT'CG_AA
Cuts at: 0 486 1710
Size: 486 1224
BstEII G'GTnAC_C
Cuts at: 0 744 867 1710
Size: 744 123 843
Fragments arranged by size:
843 744 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 556 1645 1710
Size: 237 319 1089 65
Fragments arranged by size:
1089 319 237 65
BstYI r'GATC_y
Cuts at: 0 1408 1710
Size: 1408 302
BtgI C'CryG_G
Cuts at: 0 635 935 1638 1710
Size: 635 300 703 72
Fragments arranged by size:
703 635 300 72
BtsI GCAGTG_nn'
Cuts at: 0 549 561 966 1043 1287 1444 1541 1710
Size: 549 12 405 77 244 157 97 169
Fragments arranged by size:
549 405 244 169 157 97 77 12
DraIII CAC_nnn'GTG
Cuts at: 0 658 1710
Size: 658 1052
DrdI GACnn_nn'nnGTC
Cuts at: 0 1077 1354 1710
Size: 1077 277 356
Fragments arranged by size:
1077 356 277
EaeI y'GGCC_r
Cuts at: 0 23 92 632 1203 1385 1619 1710
Size: 23 69 540 571 182 234 91
Fragments arranged by size:
571 540 234 182 91 69 23
EagI C'GGCC_G
Cuts at: 0 1619 1710
Size: 1619 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 118 1710
Size: 118 1592
EcoICRI GAG'CTC
Cuts at: 0 354 1710
Size: 354 1356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 739 1003 1051 1086 1238 1239 1344
Size: 169 570 264 48 35 152 1 105
Cuts at: 1344 1493 1508 1710
Size: 149 15 202
Fragments arranged by size:
570 264 202 169 152 149 105 48
35 15 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1033 1710
Size: 1033 677
EcoO109I rG'GnC_Cy
Cuts at: 0 212 774 1710
Size: 212 562 936
Fragments arranged by size:
936 562 212
FspI TGC'GCA
Cuts at: 0 1615 1710
Size: 1615 95
HaeII r_GCGC'y
Cuts at: 0 1244 1710
Size: 1244 466
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 226 258 527 559 1024 1056
Size: 103 32 91 32 269 32 465 32
Cuts at: 1056 1212 1244 1710
Size: 156 32 466
Fragments arranged by size:
466 465 269 156 103 91 32 32
32 32 32
MluI A'CGCG_T
Cuts at: 0 185 1710
Size: 185 1525
MslI CAynn'nnrTG
Cuts at: 0 554 694 937 1643 1710
Size: 554 140 243 706 67
Fragments arranged by size:
706 554 243 140 67
MspA1I CmG'CkG
Cuts at: 0 84 108 412 435 533 1023 1457 1710
Size: 84 24 304 23 98 490 434 253
Fragments arranged by size:
490 434 304 253 98 84 24 23
NaeI GCC'GGC
Cuts at: 0 1385 1710
Size: 1385 325
NcoI C'CATG_G
Cuts at: 0 1638 1710
Size: 1638 72
NgoMIV G'CCGG_C
Cuts at: 0 1383 1710
Size: 1383 327
NspI r_CATG'y
Cuts at: 0 594 693 1710
Size: 594 99 1017
Fragments arranged by size:
1017 594 99
PciI A'CATG_T
Cuts at: 0 590 689 1710
Size: 590 99 1021
Fragments arranged by size:
1021 590 99
PflMI CCAn_nnn'nTGG
Cuts at: 0 621 1710
Size: 621 1089
PfoI T'CCnGG_A
Cuts at: 0 731 1063 1710
Size: 731 332 647
Fragments arranged by size:
731 647 332
PmlI CAC'GTG
Cuts at: 0 804 1710
Size: 804 906
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 1710
Size: 460 32 1218
Fragments arranged by size:
1218 460 32
PpuMI rG'GwC_Cy
Cuts at: 0 774 1710
Size: 774 936
PshAI GACnn'nnGTC
Cuts at: 0 412 1710
Size: 412 1298
PspOMI G'GGCC_C
Cuts at: 0 211 1710
Size: 211 1499
PstI C_TGCA'G
Cuts at: 0 257 532 556 1265 1710
Size: 257 275 24 709 445
Fragments arranged by size:
709 445 275 257 24
PvuII CAG'CTG
Cuts at: 0 108 1023 1710
Size: 108 915 687
Fragments arranged by size:
915 687 108
SacI G_AGCT'C
Cuts at: 0 356 1710
Size: 356 1354
ScaI AGT'ACT
Cuts at: 0 880 1710
Size: 880 830
SfcI C'TryA_G
Cuts at: 0 253 528 552 1261 1305 1710
Size: 253 275 24 709 44 405
Fragments arranged by size:
709 405 275 253 44 24
SgrAI Cr'CCGG_yG
Cuts at: 0 1533 1710
Size: 1533 177
StyI C'CwwG_G
Cuts at: 0 230 1404 1638 1710
Size: 230 1174 234 72
Fragments arranged by size:
1174 234 230 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 669 763 900 906 1637 1710
Size: 669 94 137 6 731 73
Fragments arranged by size:
731 669 137 94 73 6
TatI w'GTAC_w
Cuts at: 0 680 878 990 1292 1710
Size: 680 198 112 302 418
Fragments arranged by size:
680 418 302 198 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 830 862 1418 1450 1710
Size: 830 32 556 32 260
Fragments arranged by size:
830 556 260 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 223 1710
Size: 223 1487
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlwNI
ApaI ApaLI BamHI BanI BanII BbsI BciVI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtsI DraIII DrdI EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I MluI MslI MspA1I NaeI NcoI NgoMIV NspI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuII SacI ScaI SfcI SgrAI StyI TaqII
TatI TstI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AlfI AloI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI
BcgI BclI BfrBI BglI BglII BlpI BmtI BplI
BpuEI BsaBI BsaWI BsiWI BspEI BspHI BsrDI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI EarI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MmeI MscI NarI NdeI NheI NotI NruI
NsiI PacI PasI PmeI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI XcmI XhoI XmaI XmnI
ZraI