Restriction map for
pFN21AE3522
(Linear) (Six-base) MAPSORT of: KIEE3522.seq Check: 8716 from: 1 to: 2136
KIEE3522.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AccI GT'mk_AC
Cuts at: 0 439 664 2136
Size: 439 225 1472
Fragments arranged by size:
1472 439 225
Acc65I G'GTAC_C
Cuts at: 0 1327 2136
Size: 1327 809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 847 1601 1952 2136
Size: 304 543 754 351 184
Fragments arranged by size:
754 543 351 304 184
AflIII A'CryG_T
Cuts at: 0 24 1432 2136
Size: 24 1408 704
Fragments arranged by size:
1408 704 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1928 2136
Size: 1928 208
AlwNI CAG_nnn'CTG
Cuts at: 0 1579 2136
Size: 1579 557
ApaLI G'TGCA_C
Cuts at: 0 1977 2136
Size: 1977 159
ApoI r'AATT_y
Cuts at: 0 828 852 1276 1567 2136
Size: 828 24 424 291 569
Fragments arranged by size:
828 569 424 291 24
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 974 1007 2136
Size: 974 33 1129
Fragments arranged by size:
1129 974 33
BanI G'GyrC_C
Cuts at: 0 403 693 1327 2136
Size: 403 290 634 809
Fragments arranged by size:
809 634 403 290
BanII G_rGCy'C
Cuts at: 0 656 1805 2075 2136
Size: 656 1149 270 61
Fragments arranged by size:
1149 656 270 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 955 2108 2136
Size: 955 1153 28
Fragments arranged by size:
1153 955 28
BciVI GTATCCnnnnn_n'
Cuts at: 0 6 458 2136
Size: 6 452 1678
Fragments arranged by size:
1678 452 6
BglII A'GATC_T
Cuts at: 0 955 2136
Size: 955 1181
Bme1580I G_kGCm'C
Cuts at: 0 408 1907 1981 2136
Size: 408 1499 74 155
Fragments arranged by size:
1499 408 155 74
BmtI G_CTAG'C
Cuts at: 0 7 944 2136
Size: 7 937 1192
Fragments arranged by size:
1192 937 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1023 2051 2136
Size: 296 727 1028 85
Fragments arranged by size:
1028 727 296 85
Bpu10I CC'TnA_GC
Cuts at: 0 124 1271 2131 2136
Size: 124 1147 860 5
Fragments arranged by size:
1147 860 124 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 157 1988 2136
Size: 32 125 1831 148
Fragments arranged by size:
1831 148 125 32
BsaI GGTCTCn'nnnn_
Cuts at: 0 272 1203 1813 2136
Size: 272 931 610 323
Fragments arranged by size:
931 610 323 272
BsaAI yAC'GTr
Cuts at: 0 17 1605 2136
Size: 17 1588 531
Fragments arranged by size:
1588 531 17
BsaBI GATnn'nnATC
Cuts at: 0 1896 2136
Size: 1896 240
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 768 2136
Size: 768 1368
BseYI C'CCAG_C
Cuts at: 0 1535 2136
Size: 1535 601
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1699 2136
Size: 318 1381 437
Fragments arranged by size:
1381 437 318
BsiEI CG_ry'CG
Cuts at: 0 104 2136
Size: 104 2032
BsiHKAI G_wGCw'C
Cuts at: 0 1981 2075 2136
Size: 1981 94 61
Fragments arranged by size:
1981 94 61
BsmI GAATG_Cn'
Cuts at: 0 1849 2136
Size: 1849 287
Bsp1286I G_dGCh'C
Cuts at: 0 408 656 1805 1907 1981 2075 2136
Size: 408 248 1149 102 74 94 61
Fragments arranged by size:
1149 408 248 102 94 74 61
BspHI T'CATG_A
Cuts at: 0 1146 2136
Size: 1146 990
BsrDI GCAATG_nn'
Cuts at: 0 14 1962 2136
Size: 14 1948 174
Fragments arranged by size:
1948 174 14
BstBI TT'CG_AA
Cuts at: 0 486 2136
Size: 486 1650
BstYI r'GATC_y
Cuts at: 0 955 2136
Size: 955 1181
BstZ17I GTA'TAC
Cuts at: 0 440 2136
Size: 440 1696
Bsu36I CC'TnA_GG
Cuts at: 0 1700 2136
Size: 1700 436
BtgI C'CryG_G
Cuts at: 0 365 1865 1881 2136
Size: 365 1500 16 255
Fragments arranged by size:
1500 365 255 16
BtsI GCAGTG_nn'
Cuts at: 0 330 1521 1910 1950 2136
Size: 330 1191 389 40 186
Fragments arranged by size:
1191 389 330 186 40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1156 1191 2136
Size: 1156 35 945
Fragments arranged by size:
1156 945 35
DraI TTT'AAA
Cuts at: 0 905 2136
Size: 905 1231
DraIII CAC_nnn'GTG
Cuts at: 0 87 1224 2136
Size: 87 1137 912
Fragments arranged by size:
1137 912 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 1049 2136
Size: 1049 1087
EaeI y'GGCC_r
Cuts at: 0 1562 2136
Size: 1562 574
EarI CTCTTCn'nnn_
Cuts at: 0 155 2136
Size: 155 1981
EcoICRI GAG'CTC
Cuts at: 0 2073 2136
Size: 2073 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 304 847 1023 1601 1952 2051 2136
Size: 296 8 543 176 578 351 99 85
Fragments arranged by size:
578 543 351 296 176 99 85 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1699 2136
Size: 1699 437
EcoRI G'AATT_C
Cuts at: 0 852 2136
Size: 852 1284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1438 1470 1703 1735 2076 2101 2108 2133
Size: 1438 32 233 32 341 25 7 25
Cuts at: 2133 2136
Size: 3
Fragments arranged by size:
1438 341 233 32 32 25 25 7
3
HincII GTy'rAC
Cuts at: 0 665 2136
Size: 665 1471
HindIII A'AGCT_T
Cuts at: 0 907 1583 2006 2136
Size: 907 676 423 130
Fragments arranged by size:
907 676 423 130
KpnI G_GTAC'C
Cuts at: 0 1331 2136
Size: 1331 805
MscI TGG'CCA
Cuts at: 0 1564 2136
Size: 1564 572
MslI CAynn'nnrTG
Cuts at: 0 401 1955 2096 2136
Size: 401 1554 141 40
Fragments arranged by size:
1554 401 141 40
MspA1I CmG'CkG
Cuts at: 0 36 1867 2136
Size: 36 1831 269
Fragments arranged by size:
1831 269 36
NcoI C'CATG_G
Cuts at: 0 365 1881 2136
Size: 365 1516 255
Fragments arranged by size:
1516 365 255
NheI G'CTAG_C
Cuts at: 0 3 940 2136
Size: 3 937 1196
Fragments arranged by size:
1196 937 3
NspI r_CATG'y
Cuts at: 0 28 169 1436 1445 1977 2136
Size: 28 141 1267 9 532 159
Fragments arranged by size:
1267 532 159 141 28 9
PciI A'CATG_T
Cuts at: 0 24 1432 2136
Size: 24 1408 704
Fragments arranged by size:
1408 704 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 927 2136
Size: 927 1209
PstI C_TGCA'G
Cuts at: 0 41 1816 2136
Size: 41 1775 320
Fragments arranged by size:
1775 320 41
PvuII CAG'CTG
Cuts at: 0 36 2136
Size: 36 2100
SacI G_AGCT'C
Cuts at: 0 2075 2136
Size: 2075 61
SacII CC_GC'GG
Cuts at: 0 1868 2136
Size: 1868 268
SalI G'TCGA_C
Cuts at: 0 663 2136
Size: 663 1473
SapI GCTCTTCn'nnn_
Cuts at: 0 155 2136
Size: 155 1981
ScaI AGT'ACT
Cuts at: 0 734 2136
Size: 734 1402
SfcI C'TryA_G
Cuts at: 0 37 1812 2136
Size: 37 1775 324
Fragments arranged by size:
1775 324 37
SmlI C'TyrA_G
Cuts at: 0 47 172 2003 2136
Size: 47 125 1831 133
Fragments arranged by size:
1831 133 125 47
SnaBI TAC'GTA
Cuts at: 0 1605 2136
Size: 1605 531
SphI G_CATG'C
Cuts at: 0 169 2136
Size: 169 1967
StyI C'CwwG_G
Cuts at: 0 365 1881 2136
Size: 365 1516 255
Fragments arranged by size:
1516 365 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 522 2136
Size: 118 404 1614
Fragments arranged by size:
1614 404 118
TatI w'GTAC_w
Cuts at: 0 732 1130 1656 2136
Size: 732 398 526 480
Fragments arranged by size:
732 526 480 398
Tth111I GACn'n_nGTC
Cuts at: 0 105 2136
Size: 105 2031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 142 2136
Size: 142 1994
XmnI GAAnn'nnTTC
Cuts at: 0 536 1103 1638 2136
Size: 536 567 535 498
Fragments arranged by size:
567 536 535 498
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AlwNI ApaLI ApoI
BaeI BanI BanII BbsI BciVI BglII Bme1580I BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstBI
BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoRI Hin4I
HincII HindIII KpnI MscI MslI MspA1I NcoI NheI
NspI PciI PflMI PstI PvuII SacI SacII SalI
SapI ScaI SfcI SmlI SnaBI SphI StyI TaqII
TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AloI ApaI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI
BmrI BplI BsaHI BsaWI BsaXI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BtgZI ClaI EagI EciI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI
SspI StuI SwaI TstI XbaI XhoI XmaI ZraI