Restriction map for
pFN21AE3521
(Linear) (Six-base) MAPSORT of: KIEE3521.seq Check: 6499 from: 1 to: 1365
KIEE3521.orf
With 182 enzymes: SgfI *
July 3, 2012 18:03 ..
AccI GT'mk_AC
Cuts at: 0 435 602 1365
Size: 435 167 763
Fragments arranged by size:
763 435 167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1365
Size: 1333 32
ApaLI G'TGCA_C
Cuts at: 0 1032 1365
Size: 1032 333
AvaI C'yCGr_G
Cuts at: 0 236 1365
Size: 236 1129
AvrII C'CTAG_G
Cuts at: 0 33 1365
Size: 33 1332
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 706 739 1365
Size: 706 33 626
Fragments arranged by size:
706 626 33
BanI G'GyrC_C
Cuts at: 0 37 673 1365
Size: 37 636 692
Fragments arranged by size:
692 636 37
BanII G_rGCy'C
Cuts at: 0 94 281 340 1365
Size: 94 187 59 1025
Fragments arranged by size:
1025 187 94 59
BbeI G_GCGC'C
Cuts at: 0 41 1365
Size: 41 1324
BbvCI CC'TCA_GC
Cuts at: 0 1335 1365
Size: 1335 30
BciVI GTATCCnnnnn_n'
Cuts at: 0 308 1365
Size: 308 1057
BfrBI ATG'CAT
Cuts at: 0 985 1365
Size: 985 380
Bme1580I G_kGCm'C
Cuts at: 0 1036 1365
Size: 1036 329
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 108 1365
Size: 108 1257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 412 623 1365
Size: 370 42 211 742
Fragments arranged by size:
742 370 211 42
Bpu10I CC'TnA_GC
Cuts at: 0 1335 1365
Size: 1335 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1365
Size: 430 935
BsaI GGTCTCn'nnnn_
Cuts at: 0 1222 1365
Size: 1222 143
BsaAI yAC'GTr
Cuts at: 0 545 1365
Size: 545 820
BsaHI Gr'CG_yC
Cuts at: 0 38 1365
Size: 38 1327
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 1365
Size: 160 30 1175
Fragments arranged by size:
1175 160 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 97 165 1365
Size: 93 4 68 1200
Fragments arranged by size:
1200 93 68 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1365
Size: 523 842
BsiHKAI G_wGCw'C
Cuts at: 0 94 1036 1365
Size: 94 942 329
Fragments arranged by size:
942 329 94
BsmI GAATG_Cn'
Cuts at: 0 842 985 1365
Size: 842 143 380
Fragments arranged by size:
842 380 143
BsmBI CGTCTCn'nnnn_
Cuts at: 0 143 1365
Size: 143 1222
Bsp1286I G_dGCh'C
Cuts at: 0 94 281 340 1036 1365
Size: 94 187 59 696 329
Fragments arranged by size:
696 329 187 94 59
BspHI T'CATG_A
Cuts at: 0 317 1365
Size: 317 1048
BsrDI GCAATG_nn'
Cuts at: 0 558 1365
Size: 558 807
BsrFI r'CCGG_y
Cuts at: 0 468 1365
Size: 468 897
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1069 1365
Size: 1069 296
BstYI r'GATC_y
Cuts at: 0 1064 1365
Size: 1064 301
BtgI C'CryG_G
Cuts at: 0 622 1365
Size: 622 743
BtsI GCAGTG_nn'
Cuts at: 0 400 1365
Size: 400 965
EaeI y'GGCC_r
Cuts at: 0 257 397 854 1052 1365
Size: 257 140 457 198 313
Fragments arranged by size:
457 313 257 198 140
EarI CTCTTCn'nnn_
Cuts at: 0 82 1094 1195 1352 1365
Size: 82 1012 101 157 13
Fragments arranged by size:
1012 157 101 82 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 194 205 1089 1365
Size: 194 11 884 276
Fragments arranged by size:
884 276 194 11
EcoICRI GAG'CTC
Cuts at: 0 92 1365
Size: 92 1273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 412 623 1333 1365
Size: 370 42 211 710 32
Fragments arranged by size:
710 370 211 42 32
HaeII r_GCGC'y
Cuts at: 0 41 1365
Size: 41 1324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 428 442 460 474 889 921
Size: 118 32 278 14 18 14 415 32
Cuts at: 921 1004 1036 1365
Size: 83 32 329
Fragments arranged by size:
415 329 278 118 83 32 32 32
18 14 14
HincII GTy'rAC
Cuts at: 0 715 1365
Size: 715 650
HindIII A'AGCT_T
Cuts at: 0 1358 1365
Size: 1358 7
KasI G'GCGC_C
Cuts at: 0 37 1365
Size: 37 1328
MscI TGG'CCA
Cuts at: 0 399 1365
Size: 399 966
MspA1I CmG'CkG
Cuts at: 0 9 1330 1365
Size: 9 1321 35
Fragments arranged by size:
1321 35 9
NarI GG'CG_CC
Cuts at: 0 38 1365
Size: 38 1327
NdeI CA'TA_TG
Cuts at: 0 806 1148 1365
Size: 806 342 217
Fragments arranged by size:
806 342 217
NsiI A_TGCA'T
Cuts at: 0 987 1365
Size: 987 378
NspI r_CATG'y
Cuts at: 0 1087 1365
Size: 1087 278
PvuII CAG'CTG
Cuts at: 0 9 1330 1365
Size: 9 1321 35
Fragments arranged by size:
1321 35 9
SacI G_AGCT'C
Cuts at: 0 94 1365
Size: 94 1271
SapI GCTCTTCn'nnn_
Cuts at: 0 82 1094 1365
Size: 82 1012 271
Fragments arranged by size:
1012 271 82
SfoI GGC'GCC
Cuts at: 0 39 1365
Size: 39 1326
SmaI CCC'GGG
Cuts at: 0 238 1365
Size: 238 1127
SmlI C'TyrA_G
Cuts at: 0 445 1365
Size: 445 920
SphI G_CATG'C
Cuts at: 0 1087 1365
Size: 1087 278
StyI C'CwwG_G
Cuts at: 0 33 1068 1365
Size: 33 1035 297
Fragments arranged by size:
1035 297 33
XmaI C'CCGG_G
Cuts at: 0 236 1365
Size: 236 1129
Enzymes that do cut:
AccI AcuI ApaLI AvaI AvrII BaeI BanI BanII
BbeI BbvCI BciVI BfrBI Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI BstXI BstYI
BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI HaeII
Hin4I HincII HindIII KasI MscI MspA1I NarI NdeI
NsiI NspI PvuII SacI SapI SfoI SmaI SmlI
SphI StyI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI BamHI BbsI BcgI BclI BglI BglII
BlpI BmgBI BmrI BmtI BsaBI BsaWI BseYI BsiEI
BsiWI BspEI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HpaI KpnI MfeI MluI MmeI
MslI NaeI NcoI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI