Restriction map for
pFN21AE3520
(Linear) (Six-base) MAPSORT of: KIEE3520.seq Check: 3834 from: 1 to: 879
KIEE3520.orf
With 182 enzymes: SgfI *
March 22, 2012 16:36 ..
Acc65I G'GTAC_C
Cuts at: 0 512 879
Size: 512 367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 574 879
Size: 391 183 305
Fragments arranged by size:
391 305 183
AleI CACnn'nnGTG
Cuts at: 0 571 879
Size: 571 308
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 481 513 879
Size: 481 32 366
Fragments arranged by size:
481 366 32
AscI GG'CGCG_CC
Cuts at: 0 740 879
Size: 740 139
AvaI C'yCGr_G
Cuts at: 0 331 452 526 879
Size: 331 121 74 353
Fragments arranged by size:
353 331 121 74
BanI G'GyrC_C
Cuts at: 0 512 522 757 879
Size: 512 10 235 122
Fragments arranged by size:
512 235 122 10
BanII G_rGCy'C
Cuts at: 0 311 332 879
Size: 311 21 547
Fragments arranged by size:
547 311 21
BbeI G_GCGC'C
Cuts at: 0 761 879
Size: 761 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 471 879
Size: 197 274 408
Fragments arranged by size:
408 274 197
BbvCI CC'TCA_GC
Cuts at: 0 149 879
Size: 149 730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 281 315 879
Size: 281 34 564
Fragments arranged by size:
564 281 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 655 879
Size: 79 576 224
Fragments arranged by size:
576 224 79
Bpu10I CC'TnA_GC
Cuts at: 0 149 879
Size: 149 730
BsaHI Gr'CG_yC
Cuts at: 0 758 879
Size: 758 121
BsaWI w'CCGG_w
Cuts at: 0 347 879
Size: 347 532
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 340 427 879
Size: 193 147 87 452
Fragments arranged by size:
452 193 147 87
BseYI C'CCAG_C
Cuts at: 0 721 879
Size: 721 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 879
Size: 321 558
BsiEI CG_ry'CG
Cuts at: 0 392 862 879
Size: 392 470 17
Fragments arranged by size:
470 392 17
BsiHKAI G_wGCw'C
Cuts at: 0 113 539 879
Size: 113 426 340
Fragments arranged by size:
426 340 113
BsmBI CGTCTCn'nnnn_
Cuts at: 0 379 879
Size: 379 500
Bsp1286I G_dGCh'C
Cuts at: 0 113 311 332 539 879
Size: 113 198 21 207 340
Fragments arranged by size:
340 207 198 113 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 485 587 879
Size: 485 102 292
Fragments arranged by size:
485 292 102
BssHII G'CGCG_C
Cuts at: 0 725 740 879
Size: 725 15 139
Fragments arranged by size:
725 139 15
BssSI C'ACGA_G
Cuts at: 0 297 879
Size: 297 582
BstAPI GCAn_nnn'nTGC
Cuts at: 0 710 879
Size: 710 169
BstEII G'GTnAC_C
Cuts at: 0 18 879
Size: 18 861
BtgI C'CryG_G
Cuts at: 0 81 243 823 879
Size: 81 162 580 56
Fragments arranged by size:
580 162 81 56
BtsI GCAGTG_nn'
Cuts at: 0 832 879
Size: 832 47
EaeI y'GGCC_r
Cuts at: 0 140 240 389 859 879
Size: 140 100 149 470 20
Fragments arranged by size:
470 149 140 100 20
EagI C'GGCC_G
Cuts at: 0 389 859 879
Size: 389 470 20
Fragments arranged by size:
470 389 20
EarI CTCTTCn'nnn_
Cuts at: 0 317 466 879
Size: 317 149 413
Fragments arranged by size:
413 317 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 115 424 879
Size: 115 309 455
Fragments arranged by size:
455 309 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 391 574 655 879
Size: 79 312 183 81 224
Fragments arranged by size:
312 224 183 81 79
EcoO109I rG'GnC_Cy
Cuts at: 0 456 817 879
Size: 456 361 62
Fragments arranged by size:
456 361 62
FspI TGC'GCA
Cuts at: 0 89 879
Size: 89 790
FspAI rTGC'GCAy
Cuts at: 0 89 879
Size: 89 790
HaeII r_GCGC'y
Cuts at: 0 761 767 879
Size: 761 6 112
Fragments arranged by size:
761 112 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 879
Size: 193 32 654
Fragments arranged by size:
654 193 32
KasI G'GCGC_C
Cuts at: 0 757 879
Size: 757 122
KpnI G_GTAC'C
Cuts at: 0 516 879
Size: 516 363
MslI CAynn'nnrTG
Cuts at: 0 80 571 837 879
Size: 80 491 266 42
Fragments arranged by size:
491 266 80 42
MspA1I CmG'CkG
Cuts at: 0 148 369 510 618 825 879
Size: 148 221 141 108 207 54
Fragments arranged by size:
221 207 148 141 108 54
NarI GG'CG_CC
Cuts at: 0 758 879
Size: 758 121
NcoI C'CATG_G
Cuts at: 0 81 879
Size: 81 798
NspI r_CATG'y
Cuts at: 0 138 879
Size: 138 741
PasI CC'CwG_GG
Cuts at: 0 267 879
Size: 267 612
PpuMI rG'GwC_Cy
Cuts at: 0 456 817 879
Size: 456 361 62
Fragments arranged by size:
456 361 62
PstI C_TGCA'G
Cuts at: 0 855 879
Size: 855 24
PvuII CAG'CTG
Cuts at: 0 369 879
Size: 369 510
SacII CC_GC'GG
Cuts at: 0 826 879
Size: 826 53
SfcI C'TryA_G
Cuts at: 0 747 851 879
Size: 747 104 28
Fragments arranged by size:
747 104 28
SfoI GGC'GCC
Cuts at: 0 759 879
Size: 759 120
SmaI CCC'GGG
Cuts at: 0 454 879
Size: 454 425
SphI G_CATG'C
Cuts at: 0 138 879
Size: 138 741
StuI AGG'CCT
Cuts at: 0 733 879
Size: 733 146
StyI C'CwwG_G
Cuts at: 0 81 311 879
Size: 81 230 568
Fragments arranged by size:
568 230 81
XbaI T'CTAG_A
Cuts at: 0 381 879
Size: 381 498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 79 879
Size: 79 800
XmaI C'CCGG_G
Cuts at: 0 452 879
Size: 452 427
Enzymes that do cut:
Acc65I AcuI AleI AloI AscI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BpmI Bpu10I BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BssHII BssSI BstAPI BstEII BtgI BtsI EaeI EagI
EarI EciI Eco57MI EcoO109I FspI FspAI HaeII Hin4I
KasI KpnI MslI MspA1I NarI NcoI NspI PasI
PpuMI PstI PvuII SacII SfcI SfoI SmaI SphI
StuI StyI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaXI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BstBI BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoRI EcoRV FalI FseI HincII HindIII HpaI MfeI
MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI
TaqII TatI TstI Tth111I XhoI XmnI ZraI