Restriction map for
pFN21AE3518
(Linear) (Six-base) MAPSORT of: KIEE3518.seq Check: 6130 from: 1 to: 2073
KIEE3518.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AatII G_ACGT'C
Cuts at: 0 29 2073
Size: 29 2044
AclI AA'CG_TT
Cuts at: 0 942 968 2073
Size: 942 26 1105
Fragments arranged by size:
1105 942 26
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 307 551 911 1472 2073
Size: 35 272 244 360 561 601
Fragments arranged by size:
601 561 360 272 244 35
AflII C'TTAA_G
Cuts at: 0 439 2073
Size: 439 1634
AflIII A'CryG_T
Cuts at: 0 1791 2073
Size: 1791 282
AhdI GACnn_n'nnGTC
Cuts at: 0 1125 2073
Size: 1125 948
AleI CACnn'nnGTG
Cuts at: 0 1192 2073
Size: 1192 881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 489 1628 2073
Size: 489 1139 445
Fragments arranged by size:
1139 489 445
AlwNI CAG_nnn'CTG
Cuts at: 0 1111 2073
Size: 1111 962
ApoI r'AATT_y
Cuts at: 0 1226 1416 2031 2073
Size: 1226 190 615 42
Fragments arranged by size:
1226 615 190 42
AvrII C'CTAG_G
Cuts at: 0 1735 2073
Size: 1735 338
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 977 1010 2073
Size: 977 33 1063
Fragments arranged by size:
1063 977 33
BanI G'GyrC_C
Cuts at: 0 3 2073
Size: 3 2070
BanII G_rGCy'C
Cuts at: 0 71 1221 2073
Size: 71 1150 852
Fragments arranged by size:
1150 852 71
BbeI G_GCGC'C
Cuts at: 0 7 2073
Size: 7 2066
BbsI GAAGACnn'nnnn_
Cuts at: 0 21 1144 2073
Size: 21 1123 929
Fragments arranged by size:
1123 929 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 103 137 2039 2073
Size: 103 34 1902 34
Fragments arranged by size:
1902 103 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 480 2073
Size: 480 1593
BclI T'GATC_A
Cuts at: 0 1776 2073
Size: 1776 297
BfrBI ATG'CAT
Cuts at: 0 168 2073
Size: 168 1905
BglI GCCn_nnn'nGGC
Cuts at: 0 2066 2073
Size: 2066 7
BlpI GC'TnA_GC
Cuts at: 0 265 2073
Size: 265 1808
BmgBI CAC'GTC
Cuts at: 0 1792 2073
Size: 1792 281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1093 1604 2073
Size: 1093 511 469
Fragments arranged by size:
1093 511 469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 2073
Size: 650 1423
BsaI GGTCTCn'nnnn_
Cuts at: 0 710 1849 2073
Size: 710 1139 224
Fragments arranged by size:
1139 710 224
BsaHI Gr'CG_yC
Cuts at: 0 4 26 2073
Size: 4 22 2047
Fragments arranged by size:
2047 22 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 932 962 2073
Size: 932 30 1111
Fragments arranged by size:
1111 932 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 406 1880 2073
Size: 406 1474 193
Fragments arranged by size:
1474 406 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1943 2073
Size: 1943 130
BsiHKAI G_wGCw'C
Cuts at: 0 126 1221 1889 2073
Size: 126 1095 668 184
Fragments arranged by size:
1095 668 184 126
Bsp1286I G_dGCh'C
Cuts at: 0 71 126 1221 1889 2073
Size: 71 55 1095 668 184
Fragments arranged by size:
1095 668 184 71 55
BspHI T'CATG_A
Cuts at: 0 855 2073
Size: 855 1218
BsrBI CCG'CTC
Cuts at: 0 2063 2073
Size: 2063 10
BsrDI GCAATG_nn'
Cuts at: 0 923 1709 2073
Size: 923 786 364
Fragments arranged by size:
923 786 364
BsrGI T'GTAC_A
Cuts at: 0 234 1654 2073
Size: 234 1420 419
Fragments arranged by size:
1420 419 234
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 1038 2073
Size: 414 624 1035
Fragments arranged by size:
1035 624 414
BstYI r'GATC_y
Cuts at: 0 1083 2073
Size: 1083 990
Bsu36I CC'TnA_GG
Cuts at: 0 629 2073
Size: 629 1444
BtgI C'CryG_G
Cuts at: 0 560 951 1839 2073
Size: 560 391 888 234
Fragments arranged by size:
888 560 391 234
BtsI GCAGTG_nn'
Cuts at: 0 471 2044 2073
Size: 471 1573 29
Fragments arranged by size:
1573 471 29
DraI TTT'AAA
Cuts at: 0 552 2073
Size: 552 1521
EarI CTCTTCn'nnn_
Cuts at: 0 131 383 428 527 887 2073
Size: 131 252 45 99 360 1186
Fragments arranged by size:
1186 360 252 131 99 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 63 2073
Size: 63 2010
EcoICRI GAG'CTC
Cuts at: 0 1219 2073
Size: 1219 854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 307 551 911 1093 1472 1604 2073
Size: 35 272 244 360 182 379 132 469
Fragments arranged by size:
469 379 360 272 244 182 132 35
EcoRI G'AATT_C
Cuts at: 0 1226 2073
Size: 1226 847
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 866 2073
Size: 866 1207
HaeII r_GCGC'y
Cuts at: 0 7 2073
Size: 7 2066
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 556 588 1111 1143 1224 1256 2073
Size: 556 32 523 32 81 32 817
Fragments arranged by size:
817 556 523 81 32 32 32
HindIII A'AGCT_T
Cuts at: 0 82 2073
Size: 82 1991
KasI G'GCGC_C
Cuts at: 0 3 2073
Size: 3 2070
MfeI C'AATT_G
Cuts at: 0 911 2073
Size: 911 1162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 1556 1557 2073
Size: 128 1428 1 516
Fragments arranged by size:
1428 516 128 1
MslI CAynn'nnrTG
Cuts at: 0 412 1192 1244 1309 1496 2073
Size: 412 780 52 65 187 577
Fragments arranged by size:
780 577 412 187 65 52
MspA1I CmG'CkG
Cuts at: 0 60 711 2073
Size: 60 651 1362
Fragments arranged by size:
1362 651 60
NarI GG'CG_CC
Cuts at: 0 4 2073
Size: 4 2069
NcoI C'CATG_G
Cuts at: 0 951 1839 2073
Size: 951 888 234
Fragments arranged by size:
951 888 234
NdeI CA'TA_TG
Cuts at: 0 978 2073
Size: 978 1095
NsiI A_TGCA'T
Cuts at: 0 170 2073
Size: 170 1903
NspI r_CATG'y
Cuts at: 0 1952 2073
Size: 1952 121
PflMI CCAn_nnn'nTGG
Cuts at: 0 982 2073
Size: 982 1091
PshAI GACnn'nnGTC
Cuts at: 0 1591 1841 2073
Size: 1591 250 232
Fragments arranged by size:
1591 250 232
PsiI TTA'TAA
Cuts at: 0 230 2073
Size: 230 1843
PstI C_TGCA'G
Cuts at: 0 134 495 1026 1934 2073
Size: 134 361 531 908 139
Fragments arranged by size:
908 531 361 139 134
PvuII CAG'CTG
Cuts at: 0 60 711 2073
Size: 60 651 1362
Fragments arranged by size:
1362 651 60
SacI G_AGCT'C
Cuts at: 0 1221 2073
Size: 1221 852
SapI GCTCTTCn'nnn_
Cuts at: 0 131 428 527 2073
Size: 131 297 99 1546
Fragments arranged by size:
1546 297 131 99
SbfI CC_TGCA'GG
Cuts at: 0 1934 2073
Size: 1934 139
SfcI C'TryA_G
Cuts at: 0 130 491 1022 1930 2073
Size: 130 361 531 908 143
Fragments arranged by size:
908 531 361 143 130
SfoI GGC'GCC
Cuts at: 0 5 2073
Size: 5 2068
SmlI C'TyrA_G
Cuts at: 0 439 665 2073
Size: 439 226 1408
Fragments arranged by size:
1408 439 226
SpeI A'CTAG_T
Cuts at: 0 1588 2073
Size: 1588 485
SspI AAT'ATT
Cuts at: 0 421 556 2073
Size: 421 135 1517
Fragments arranged by size:
1517 421 135
StuI AGG'CCT
Cuts at: 0 1928 2073
Size: 1928 145
StyI C'CwwG_G
Cuts at: 0 951 1735 1839 2073
Size: 951 784 104 234
Fragments arranged by size:
951 784 234 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 810 2073
Size: 810 1263
TatI w'GTAC_w
Cuts at: 0 234 1654 1794 2073
Size: 234 1420 140 279
Fragments arranged by size:
1420 279 234 140
ZraI GAC'GTC
Cuts at: 0 27 2073
Size: 27 2046
Enzymes that do cut:
AatII AclI AcuI AflII AflIII AhdI AleI AlfI
AlwNI ApoI AvrII BaeI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BlpI BmgBI BpmI
BpuEI BsaI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BspHI BsrBI BsrDI BsrGI BstXI BstYI Bsu36I BtgI
BtsI DraI EarI EciI EcoICRI Eco57MI EcoRI FalI
HaeII Hin4I HindIII KasI MfeI MmeI MslI MspA1I
NarI NcoI NdeI NsiI NspI PflMI PshAI PsiI
PstI PvuII SacI SapI SbfI SfcI SfoI SmlI
SpeI SspI StuI StyI TaqII TatI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AgeI AloI ApaI ApaLI
AscI AseI AsiSI AvaI BamHI BbvCI BglII Bme1580I
BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EcoNI EcoO109I EcoRV FseI
FspI FspAI HincII HpaI KpnI MluI MscI NaeI
NgoMIV NheI NotI NruI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI