Restriction map for
pFN21AE3515
(Linear) (Six-base) MAPSORT of: KIEE3515.seq Check: 2568 from: 1 to: 2904
KIEE3515.orf
With 182 enzymes: SgfI *
July 3, 2012 18:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2863 2904
Size: 2863 41
AccI GT'mk_AC
Cuts at: 0 1040 2904
Size: 1040 1864
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1860 2075 2644 2904
Size: 923 937 215 569 260
Fragments arranged by size:
937 923 569 260 215
AleI CACnn'nnGTG
Cuts at: 0 781 2794 2904
Size: 781 2013 110
Fragments arranged by size:
2013 781 110
AlwNI CAG_nnn'CTG
Cuts at: 0 1614 2904
Size: 1614 1290
ApaI G_GGCC'C
Cuts at: 0 484 1079 2904
Size: 484 595 1825
Fragments arranged by size:
1825 595 484
ApoI r'AATT_y
Cuts at: 0 1330 1481 1723 2904
Size: 1330 151 242 1181
Fragments arranged by size:
1330 1181 242 151
AvaI C'yCGr_G
Cuts at: 0 34 75 126 357 447 554 2904
Size: 34 41 51 231 90 107 2350
Fragments arranged by size:
2350 231 107 90 51 41 34
BamHI G'GATC_C
Cuts at: 0 985 2904
Size: 985 1919
BanI G'GyrC_C
Cuts at: 0 109 353 461 2904
Size: 109 244 108 2443
Fragments arranged by size:
2443 244 109 108
BanII G_rGCy'C
Cuts at: 0 397 484 730 1068 1079 2904
Size: 397 87 246 338 11 1825
Fragments arranged by size:
1825 397 338 246 87 11
BbeI G_GCGC'C
Cuts at: 0 113 357 465 2904
Size: 113 244 108 2439
Fragments arranged by size:
2439 244 113 108
BbsI GAAGACnn'nnnn_
Cuts at: 0 812 836 2904
Size: 812 24 2068
Fragments arranged by size:
2068 812 24
BbvCI CC'TCA_GC
Cuts at: 0 26 2904
Size: 26 2878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1848 1882 2904
Size: 1848 34 1022
Fragments arranged by size:
1848 1022 34
BglI GCCn_nnn'nGGC
Cuts at: 0 385 503 2904
Size: 385 118 2401
Fragments arranged by size:
2401 385 118
BglII A'GATC_T
Cuts at: 0 2103 2229 2904
Size: 2103 126 675
Fragments arranged by size:
2103 675 126
Bme1580I G_kGCm'C
Cuts at: 0 484 493 1079 2904
Size: 484 9 586 1825
Fragments arranged by size:
1825 586 484 9
BmgBI CAC'GTC
Cuts at: 0 2392 2904
Size: 2392 512
BmrI ACTGGGnnnn_n'
Cuts at: 0 493 1146 2904
Size: 493 653 1758
Fragments arranged by size:
1758 653 493
BmtI G_CTAG'C
Cuts at: 0 1223 2904
Size: 1223 1681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 2454 2904
Size: 175 2279 450
Fragments arranged by size:
2279 450 175
Bpu10I CC'TnA_GC
Cuts at: 0 26 2904
Size: 26 2878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1029 1210 2904
Size: 1029 181 1694
Fragments arranged by size:
1694 1029 181
BsaI GGTCTCn'nnnn_
Cuts at: 0 601 2291 2904
Size: 601 1690 613
Fragments arranged by size:
1690 613 601
BsaAI yAC'GTr
Cuts at: 0 2728 2901 2904
Size: 2728 173 3
Fragments arranged by size:
2728 173 3
BsaBI GATnn'nnATC
Cuts at: 0 2372 2904
Size: 2372 532
BsaHI Gr'CG_yC
Cuts at: 0 41 110 354 462 2582 2904
Size: 41 69 244 108 2120 322
Fragments arranged by size:
2120 322 244 108 69 41
BsaWI w'CCGG_w
Cuts at: 0 2540 2904
Size: 2540 364
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 953 983 2904
Size: 953 30 1921
Fragments arranged by size:
1921 953 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 218 304 307 310 313 316 372 2210
Size: 218 86 3 3 3 3 56 1838
Cuts at: 2210 2452 2643 2904
Size: 242 191 261
Fragments arranged by size:
1838 261 242 218 191 86 56 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 493 889 1335 2640 2679 2744 2904
Size: 493 396 446 1305 39 65 160
Fragments arranged by size:
1305 493 446 396 160 65 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1193 2891 2904
Size: 40 1153 1698 13
Fragments arranged by size:
1698 1153 40 13
BsiEI CG_ry'CG
Cuts at: 0 52 104 188 206 377 389 2904
Size: 52 52 84 18 171 12 2515
Fragments arranged by size:
2515 171 84 52 52 18 12
BsiHKAI G_wGCw'C
Cuts at: 0 247 397 730 2904
Size: 247 150 333 2174
Fragments arranged by size:
2174 333 247 150
BsmI GAATG_Cn'
Cuts at: 0 1487 2550 2904
Size: 1487 1063 354
Fragments arranged by size:
1487 1063 354
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2330 2523 2904
Size: 2330 193 381
Fragments arranged by size:
2330 381 193
Bsp1286I G_dGCh'C
Cuts at: 0 247 397 484 493 730 1068 1079 2904
Size: 247 150 87 9 237 338 11 1825
Fragments arranged by size:
1825 338 247 237 150 87 11 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 947 2863 2904
Size: 947 1916 41
Fragments arranged by size:
1916 947 41
BsrBI CCG'CTC
Cuts at: 0 234 452 2904
Size: 234 218 2452
Fragments arranged by size:
2452 234 218
BsrDI GCAATG_nn'
Cuts at: 0 1145 2904
Size: 1145 1759
BsrFI r'CCGG_y
Cuts at: 0 80 464 2904
Size: 80 384 2440
Fragments arranged by size:
2440 384 80
BsrGI T'GTAC_A
Cuts at: 0 1283 2904
Size: 1283 1621
BssHII G'CGCG_C
Cuts at: 0 65 404 2904
Size: 65 339 2500
Fragments arranged by size:
2500 339 65
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1216 2904
Size: 1216 1688
BstYI r'GATC_y
Cuts at: 0 985 1097 2103 2229 2904
Size: 985 112 1006 126 675
Fragments arranged by size:
1006 985 675 126 112
BtgI C'CryG_G
Cuts at: 0 104 212 398 432 824 2162 2904
Size: 104 108 186 34 392 1338 742
Fragments arranged by size:
1338 742 392 186 108 104 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 508 2904
Size: 508 2396
DraI TTT'AAA
Cuts at: 0 810 1379 2904
Size: 810 569 1525
Fragments arranged by size:
1525 810 569
DrdI GACnn_nn'nnGTC
Cuts at: 0 1767 2807 2904
Size: 1767 1040 97
Fragments arranged by size:
1767 1040 97
EaeI y'GGCC_r
Cuts at: 0 49 101 185 203 374 386 2663 2904
Size: 49 52 84 18 171 12 2277 241
Fragments arranged by size:
2277 241 171 84 52 49 18 12
EagI C'GGCC_G
Cuts at: 0 49 101 185 203 374 386 2904
Size: 49 52 84 18 171 12 2518
Fragments arranged by size:
2518 171 84 52 49 18 12
EarI CTCTTCn'nnn_
Cuts at: 0 772 1463 1723 2504 2620 2726 2904
Size: 772 691 260 781 116 106 178
Fragments arranged by size:
781 772 691 260 178 116 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 338 583 2904
Size: 338 245 2321
Fragments arranged by size:
2321 338 245
EcoICRI GAG'CTC
Cuts at: 0 395 728 2904
Size: 395 333 2176
Fragments arranged by size:
2176 395 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 923 1860 2075 2454 2644 2904
Size: 175 748 937 215 379 190 260
Fragments arranged by size:
937 748 379 260 215 190 175
EcoO109I rG'GnC_Cy
Cuts at: 0 150 195 480 513 1076 2361 2904
Size: 150 45 285 33 563 1285 543
Fragments arranged by size:
1285 563 543 285 150 45 33
EcoRI G'AATT_C
Cuts at: 0 1330 2904
Size: 1330 1574
EcoRV GAT'ATC
Cuts at: 0 1929 2904
Size: 1929 975
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2226 2904
Size: 2226 678
HaeII r_GCGC'y
Cuts at: 0 113 357 465 2904
Size: 113 244 108 2439
Fragments arranged by size:
2439 244 113 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1027 1059 2035 2067 2216 2248 2904
Size: 1027 32 976 32 149 32 656
Fragments arranged by size:
1027 976 656 149 32 32 32
HincII GTy'rAC
Cuts at: 0 2812 2904
Size: 2812 92
HindIII A'AGCT_T
Cuts at: 0 2129 2904
Size: 2129 775
KasI G'GCGC_C
Cuts at: 0 109 353 461 2904
Size: 109 244 108 2443
Fragments arranged by size:
2443 244 109 108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2633 2717 2904
Size: 2633 84 187
Fragments arranged by size:
2633 187 84
MslI CAynn'nnrTG
Cuts at: 0 751 781 2252 2794 2904
Size: 751 30 1471 542 110
Fragments arranged by size:
1471 751 542 110 30
MspA1I CmG'CkG
Cuts at: 0 147 214 519 1207 2904
Size: 147 67 305 688 1697
Fragments arranged by size:
1697 688 305 147 67
NaeI GCC'GGC
Cuts at: 0 82 466 2904
Size: 82 384 2438
Fragments arranged by size:
2438 384 82
NarI GG'CG_CC
Cuts at: 0 110 354 462 2904
Size: 110 244 108 2442
Fragments arranged by size:
2442 244 110 108
NcoI C'CATG_G
Cuts at: 0 824 2904
Size: 824 2080
NgoMIV G'CCGG_C
Cuts at: 0 80 464 2904
Size: 80 384 2440
Fragments arranged by size:
2440 384 80
NheI G'CTAG_C
Cuts at: 0 1219 2904
Size: 1219 1685
NotI GC'GGCC_GC
Cuts at: 0 49 185 2904
Size: 49 136 2719
Fragments arranged by size:
2719 136 49
PpuMI rG'GwC_Cy
Cuts at: 0 195 513 2361 2904
Size: 195 318 1848 543
Fragments arranged by size:
1848 543 318 195
PspOMI G'GGCC_C
Cuts at: 0 480 1075 2904
Size: 480 595 1829
Fragments arranged by size:
1829 595 480
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2708 2740 2904
Size: 2708 32 164
Fragments arranged by size:
2708 164 32
PvuII CAG'CTG
Cuts at: 0 1207 2904
Size: 1207 1697
SacI G_AGCT'C
Cuts at: 0 397 730 2904
Size: 397 333 2174
Fragments arranged by size:
2174 397 333
SacII CC_GC'GG
Cuts at: 0 215 2904
Size: 215 2689
SanDI GG'GwC_CC
Cuts at: 0 195 513 2904
Size: 195 318 2391
Fragments arranged by size:
2391 318 195
SapI GCTCTTCn'nnn_
Cuts at: 0 772 1723 2904
Size: 772 951 1181
Fragments arranged by size:
1181 951 772
ScaI AGT'ACT
Cuts at: 0 1881 2904
Size: 1881 1023
SfcI C'TryA_G
Cuts at: 0 1562 1606 2852 2904
Size: 1562 44 1246 52
Fragments arranged by size:
1562 1246 52 44
SfoI GGC'GCC
Cuts at: 0 111 355 463 2904
Size: 111 244 108 2441
Fragments arranged by size:
2441 244 111 108
SgrAI Cr'CCGG_yG
Cuts at: 0 464 2904
Size: 464 2440
SmaI CCC'GGG
Cuts at: 0 36 77 128 359 2904
Size: 36 41 51 231 2545
Fragments arranged by size:
2545 231 51 41 36
SmlI C'TyrA_G
Cuts at: 0 1008 1225 2904
Size: 1008 217 1679
Fragments arranged by size:
1679 1008 217
SrfI GCCC'GGGC
Cuts at: 0 359 2904
Size: 359 2545
SspI AAT'ATT
Cuts at: 0 2273 2904
Size: 2273 631
StyI C'CwwG_G
Cuts at: 0 824 2904
Size: 824 2080
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1262 2326 2904
Size: 1262 1064 578
Fragments arranged by size:
1262 1064 578
TatI w'GTAC_w
Cuts at: 0 737 872 1283 1879 2904
Size: 737 135 411 596 1025
Fragments arranged by size:
1025 737 596 411 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 317 334 349 366 752 784 2904
Size: 317 17 15 17 386 32 2120
Fragments arranged by size:
2120 386 317 32 17 17 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2776 2904
Size: 2776 128
XmaI C'CCGG_G
Cuts at: 0 34 75 126 357 2904
Size: 34 41 51 231 2547
Fragments arranged by size:
2547 231 51 41 34
XmnI GAAnn'nnTTC
Cuts at: 0 2487 2513 2904
Size: 2487 26 391
Fragments arranged by size:
2487 391 26
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
BglII Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstYI BtgI BtgZI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI PpuMI PspOMI PsrI PvuII SacI SacII
SanDI SapI ScaI SfcI SfoI SgrAI SmaI SmlI
SrfI SspI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BciVI BclI BfrBI BlpI BplI BsiWI BspEI BspHI
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI
CspCI DraIII EcoNI FseI FspI FspAI HpaI KpnI
MfeI MluI MscI NdeI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PstI PvuI RsrII SalI SbfI SexAI
SfiI SgfI SnaBI SpeI SphI StuI SwaI Tth111I
XbaI XhoI ZraI