Restriction map for
pFN21AE3512
(Linear) (Six-base) MAPSORT of: KIEE3512.seq Check: 365 from: 1 to: 1836
KIEE3512.orf
With 182 enzymes: SgfI *
July 3, 2012 18:02 ..
AccI GT'mk_AC
Cuts at: 0 1304 1836
Size: 1304 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 663 747 1419 1836
Size: 663 84 672 417
Fragments arranged by size:
672 663 417 84
AleI CACnn'nnGTG
Cuts at: 0 806 1394 1478 1836
Size: 806 588 84 358
Fragments arranged by size:
806 588 358 84
ApoI r'AATT_y
Cuts at: 0 545 855 881 965 1133 1217 1836
Size: 545 310 26 84 168 84 619
Fragments arranged by size:
619 545 310 168 84 84 26
AvaI C'yCGr_G
Cuts at: 0 9 1614 1836
Size: 9 1605 222
Fragments arranged by size:
1605 222 9
BamHI G'GATC_C
Cuts at: 0 237 1049 1836
Size: 237 812 787
Fragments arranged by size:
812 787 237
BanII G_rGCy'C
Cuts at: 0 16 607 1027 1447 1836
Size: 16 591 420 420 389
Fragments arranged by size:
591 420 420 389 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1240 1836
Size: 1240 596
BmrI ACTGGGnnnn_n'
Cuts at: 0 563 899 1836
Size: 563 336 937
Fragments arranged by size:
937 563 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 1836
Size: 659 1177
Bpu10I CC'TnA_GC
Cuts at: 0 1194 1836
Size: 1194 642
BsaI GGTCTCn'nnnn_
Cuts at: 0 114 1479 1836
Size: 114 1365 357
Fragments arranged by size:
1365 357 114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 157 1836
Size: 49 108 1679
Fragments arranged by size:
1679 108 49
BseYI C'CCAG_C
Cuts at: 0 54 1836
Size: 54 1782
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1518 1836
Size: 1518 318
BsiHKAI G_wGCw'C
Cuts at: 0 607 1027 1365 1447 1836
Size: 607 420 338 82 389
Fragments arranged by size:
607 420 389 338 82
Bsp1286I G_dGCh'C
Cuts at: 0 16 607 1027 1365 1447 1836
Size: 16 591 420 338 82 389
Fragments arranged by size:
591 420 389 338 82 16
BspHI T'CATG_A
Cuts at: 0 83 1836
Size: 83 1753
BsrDI GCAATG_nn'
Cuts at: 0 498 859 1836
Size: 498 361 977
Fragments arranged by size:
977 498 361
BsrGI T'GTAC_A
Cuts at: 0 1371 1836
Size: 1371 465
BstXI CCAn_nnnn'nTGG
Cuts at: 0 910 1836
Size: 910 926
BstYI r'GATC_y
Cuts at: 0 237 293 1049 1721 1836
Size: 237 56 756 672 115
Fragments arranged by size:
756 672 237 115 56
Bsu36I CC'TnA_GG
Cuts at: 0 1310 1836
Size: 1310 526
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 480 515 1068 1103 1836
Size: 480 35 553 35 733
Fragments arranged by size:
733 553 480 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 199 1836
Size: 199 1637
EarI CTCTTCn'nnn_
Cuts at: 0 383 835 1836
Size: 383 452 1001
Fragments arranged by size:
1001 452 383
EcoICRI GAG'CTC
Cuts at: 0 605 1025 1445 1836
Size: 605 420 420 391
Fragments arranged by size:
605 420 420 391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 663 747 1419 1836
Size: 659 4 84 672 417
Fragments arranged by size:
672 659 417 84 4
EcoO109I rG'GnC_Cy
Cuts at: 0 563 1836
Size: 563 1273
EcoRI G'AATT_C
Cuts at: 0 545 881 1133 1217 1836
Size: 545 336 252 84 619
Fragments arranged by size:
619 545 336 252 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 338 861 1836
Size: 338 523 975
Fragments arranged by size:
975 523 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 834 866 1476 1508 1836
Size: 834 32 610 32 328
Fragments arranged by size:
834 610 328 32 32
HincII GTy'rAC
Cuts at: 0 1693 1710 1836
Size: 1693 17 126
Fragments arranged by size:
1693 126 17
HindIII A'AGCT_T
Cuts at: 0 1431 1515 1836
Size: 1431 84 321
Fragments arranged by size:
1431 321 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1000 1836
Size: 748 252 836
Fragments arranged by size:
836 748 252
MslI CAynn'nnrTG
Cuts at: 0 404 470 722 806 908 1058 1160 1394
Size: 404 66 252 84 102 150 102 234
Cuts at: 1394 1478 1550 1580 1757 1836
Size: 84 72 30 177 79
Fragments arranged by size:
404 252 234 177 150 102 102 84
84 79 72 66 30
PsiI TTA'TAA
Cuts at: 0 1325 1661 1836
Size: 1325 336 175
Fragments arranged by size:
1325 336 175
SacI G_AGCT'C
Cuts at: 0 607 1027 1447 1836
Size: 607 420 420 389
Fragments arranged by size:
607 420 420 389
SapI GCTCTTCn'nnn_
Cuts at: 0 835 1836
Size: 835 1001
SexAI A'CCwGG_T
Cuts at: 0 104 1836
Size: 104 1732
StuI AGG'CCT
Cuts at: 0 1600 1836
Size: 1600 236
StyI C'CwwG_G
Cuts at: 0 313 1836
Size: 313 1523
TatI w'GTAC_w
Cuts at: 0 1371 1414 1836
Size: 1371 43 422
Fragments arranged by size:
1371 422 43
XmnI GAAnn'nnTTC
Cuts at: 0 760 1096 1432 1836
Size: 760 336 336 404
Fragments arranged by size:
760 404 336 336
Enzymes that do cut:
AccI AcuI AleI ApoI AvaI BamHI BanII BbsI
BmrI BpmI Bpu10I BsaI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BsrDI BsrGI BstXI BstYI Bsu36I CspCI
DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I
HincII HindIII MmeI MslI PsiI SacI SapI SexAI
StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EciI EcoNI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI