Restriction map for
pFN21AE3508
(Linear) (Six-base) MAPSORT of: KIEE3508.seq Check: 2650 from: 1 to: 2109
KIEE3508.orf
With 182 enzymes: SgfI *
July 3, 2012 18:01 ..
Acc65I G'GTAC_C
Cuts at: 0 188 2109
Size: 188 1921
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1151 1940 2109
Size: 1151 789 169
Fragments arranged by size:
1151 789 169
AfeI AGC'GCT
Cuts at: 0 1517 2109
Size: 1517 592
AflIII A'CryG_T
Cuts at: 0 1571 2109
Size: 1571 538
AleI CACnn'nnGTG
Cuts at: 0 1315 2109
Size: 1315 794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 479 1411 2109
Size: 479 932 698
Fragments arranged by size:
932 698 479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2075 2107 2109
Size: 2075 32 2
Fragments arranged by size:
2075 32 2
AlwNI CAG_nnn'CTG
Cuts at: 0 64 378 1060 2109
Size: 64 314 682 1049
Fragments arranged by size:
1049 682 314 64
ApaI G_GGCC'C
Cuts at: 0 928 1128 1816 2109
Size: 928 200 688 293
Fragments arranged by size:
928 688 293 200
AvaI C'yCGr_G
Cuts at: 0 38 206 511 686 2109
Size: 38 168 305 175 1423
Fragments arranged by size:
1423 305 175 168 38
BanI G'GyrC_C
Cuts at: 0 20 188 463 1597 1669 2011 2109
Size: 20 168 275 1134 72 342 98
Fragments arranged by size:
1134 342 275 168 98 72 20
BanII G_rGCy'C
Cuts at: 0 152 626 769 875 928 1128 1816 1823
Size: 152 474 143 106 53 200 688 7
Cuts at: 1823 1958 2109
Size: 135 151
Fragments arranged by size:
688 474 200 152 151 143 135 106
53 7
BbvCI CC'TCA_GC
Cuts at: 0 712 2109
Size: 712 1397
BciVI GTATCCnnnnn_n'
Cuts at: 0 1331 2109
Size: 1331 778
BglI GCCn_nnn'nGGC
Cuts at: 0 572 581 2109
Size: 572 9 1528
Fragments arranged by size:
1528 572 9
BlpI GC'TnA_GC
Cuts at: 0 1741 2109
Size: 1741 368
Bme1580I G_kGCm'C
Cuts at: 0 25 410 711 897 928 1128 1602 1816
Size: 25 385 301 186 31 200 474 214
Cuts at: 1816 2109
Size: 293
Fragments arranged by size:
474 385 301 293 214 200 186 31
25
BmrI ACTGGGnnnn_n'
Cuts at: 0 1149 1351 2109
Size: 1149 202 758
Fragments arranged by size:
1149 758 202
BmtI G_CTAG'C
Cuts at: 0 557 2109
Size: 557 1552
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1812 2109
Size: 1812 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1006 1053 1258 1648 1807 1888 1981
Size: 71 935 47 205 390 159 81 93
Cuts at: 1981 2082 2109
Size: 101 27
Fragments arranged by size:
935 390 205 159 101 93 81 71
47 27
Bpu10I CC'TnA_GC
Cuts at: 0 712 1817 2109
Size: 712 1105 292
Fragments arranged by size:
1105 712 292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1678 1690 2109
Size: 1678 12 419
Fragments arranged by size:
1678 419 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 219 1057 2109
Size: 214 5 838 1052
Fragments arranged by size:
1052 838 214 5
BsaAI yAC'GTr
Cuts at: 0 293 405 2109
Size: 293 112 1704
Fragments arranged by size:
1704 293 112
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 160 1519 2109
Size: 157 3 1359 590
Fragments arranged by size:
1359 590 157 3
BseYI C'CCAG_C
Cuts at: 0 30 875 1382 2109
Size: 30 845 507 727
Fragments arranged by size:
845 727 507 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1473 1507 2109
Size: 721 752 34 602
Fragments arranged by size:
752 721 602 34
BsiEI CG_ry'CG
Cuts at: 0 1547 1951 2109
Size: 1547 404 158
Fragments arranged by size:
1547 404 158
BsiHKAI G_wGCw'C
Cuts at: 0 152 290 1841 1958 2109
Size: 152 138 1551 117 151
Fragments arranged by size:
1551 152 151 138 117
Bsp1286I G_dGCh'C
Cuts at: 0 25 152 290 410 626 711 769 875
Size: 25 127 138 120 216 85 58 106
Cuts at: 875 897 928 1128 1602 1816 1823 1841 1958
Size: 22 31 200 474 214 7 18 117
Cuts at: 1958 2109
Size: 151
Fragments arranged by size:
474 216 214 200 151 138 127 120
117 106 85 58 31 25 22 18
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 743 1442 2094 2109
Size: 743 699 652 15
Fragments arranged by size:
743 699 652 15
BsrBI CCG'CTC
Cuts at: 0 1509 2096 2109
Size: 1509 587 13
Fragments arranged by size:
1509 587 13
BsrDI GCAATG_nn'
Cuts at: 0 835 2109
Size: 835 1274
BsrFI r'CCGG_y
Cuts at: 0 1229 1415 1492 2109
Size: 1229 186 77 617
Fragments arranged by size:
1229 617 186 77
BssSI C'ACGA_G
Cuts at: 0 415 2109
Size: 415 1694
BstAPI GCAn_nnn'nTGC
Cuts at: 0 841 2109
Size: 841 1268
BstEII G'GTnAC_C
Cuts at: 0 639 1641 2109
Size: 639 1002 468
Fragments arranged by size:
1002 639 468
BstXI CCAn_nnnn'nTGG
Cuts at: 0 952 1467 1539 2109
Size: 952 515 72 570
Fragments arranged by size:
952 570 515 72
BstYI r'GATC_y
Cuts at: 0 168 725 2109
Size: 168 557 1384
Fragments arranged by size:
1384 557 168
Bsu36I CC'TnA_GG
Cuts at: 0 570 2109
Size: 570 1539
BtgI C'CryG_G
Cuts at: 0 192 906 1410 1472 1893 2109
Size: 192 714 504 62 421 216
Fragments arranged by size:
714 504 421 216 192 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 98 665 2109
Size: 98 567 1444
Fragments arranged by size:
1444 567 98
BtsI GCAGTG_nn'
Cuts at: 0 499 626 2060 2109
Size: 499 127 1434 49
Fragments arranged by size:
1434 499 127 49
DraIII CAC_nnn'GTG
Cuts at: 0 1845 2109
Size: 1845 264
EaeI y'GGCC_r
Cuts at: 0 195 1005 1193 1231 1413 1469 1544 1948
Size: 195 810 188 38 182 56 75 404
Cuts at: 1948 2109
Size: 161
Fragments arranged by size:
810 404 195 188 182 161 75 56
38
EagI C'GGCC_G
Cuts at: 0 1544 1948 2109
Size: 1544 404 161
Fragments arranged by size:
1544 404 161
EcoICRI GAG'CTC
Cuts at: 0 150 1956 2109
Size: 150 1806 153
Fragments arranged by size:
1806 153 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1006 1053 1151 1258 1648 1807 1888
Size: 71 935 47 98 107 390 159 81
Cuts at: 1888 1940 1981 2082 2109
Size: 52 41 101 27
Fragments arranged by size:
935 390 159 107 101 98 81 71
52 47 41 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 532 955 1717 2109
Size: 532 423 762 392
Fragments arranged by size:
762 532 423 392
EcoO109I rG'GnC_Cy
Cuts at: 0 35 574 924 925 1124 1125 1812 1813
Size: 35 539 350 1 199 1 687 1
Cuts at: 1813 2055 2109
Size: 242 54
Fragments arranged by size:
687 539 350 242 199 54 35 1
1 1
FspI TGC'GCA
Cuts at: 0 1255 2109
Size: 1255 854
HaeII r_GCGC'y
Cuts at: 0 1519 2037 2109
Size: 1519 518 72
Fragments arranged by size:
1519 518 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 724 756 985 1017 2109
Size: 724 32 229 32 1092
Fragments arranged by size:
1092 724 229 32 32
KpnI G_GTAC'C
Cuts at: 0 192 2109
Size: 192 1917
MscI TGG'CCA
Cuts at: 0 1007 2109
Size: 1007 1102
MslI CAynn'nnrTG
Cuts at: 0 1315 1913 2109
Size: 1315 598 196
Fragments arranged by size:
1315 598 196
MspA1I CmG'CkG
Cuts at: 0 299 704 879 1382 1539 1740 2109
Size: 299 405 175 503 157 201 369
Fragments arranged by size:
503 405 369 299 201 175 157
NaeI GCC'GGC
Cuts at: 0 1417 2109
Size: 1417 692
NcoI C'CATG_G
Cuts at: 0 192 906 1410 2109
Size: 192 714 504 699
Fragments arranged by size:
714 699 504 192
NgoMIV G'CCGG_C
Cuts at: 0 1415 2109
Size: 1415 694
NheI G'CTAG_C
Cuts at: 0 553 2109
Size: 553 1556
NspI r_CATG'y
Cuts at: 0 1309 1575 2109
Size: 1309 266 534
Fragments arranged by size:
1309 534 266
PasI CC'CwG_GG
Cuts at: 0 1220 1823 2109
Size: 1220 603 286
Fragments arranged by size:
1220 603 286
PciI A'CATG_T
Cuts at: 0 1571 2109
Size: 1571 538
PflMI CCAn_nnn'nTGG
Cuts at: 0 64 2079 2109
Size: 64 2015 30
Fragments arranged by size:
2015 64 30
PfoI T'CCnGG_A
Cuts at: 0 104 254 885 1958 2109
Size: 104 150 631 1073 151
Fragments arranged by size:
1073 631 151 150 104
PmlI CAC'GTG
Cuts at: 0 293 405 2109
Size: 293 112 1704
Fragments arranged by size:
1704 293 112
PpuMI rG'GwC_Cy
Cuts at: 0 35 2055 2109
Size: 35 2020 54
Fragments arranged by size:
2020 54 35
PspOMI G'GGCC_C
Cuts at: 0 924 1124 1812 2109
Size: 924 200 688 297
Fragments arranged by size:
924 688 297 200
PspXI vC'TCGA_Gb
Cuts at: 0 511 2109
Size: 511 1598
PstI C_TGCA'G
Cuts at: 0 819 1934 2109
Size: 819 1115 175
Fragments arranged by size:
1115 819 175
PvuII CAG'CTG
Cuts at: 0 704 879 1539 2109
Size: 704 175 660 570
Fragments arranged by size:
704 660 570 175
SacI G_AGCT'C
Cuts at: 0 152 1958 2109
Size: 152 1806 151
Fragments arranged by size:
1806 152 151
SanDI GG'GwC_CC
Cuts at: 0 35 2109
Size: 35 2074
SfcI C'TryA_G
Cuts at: 0 783 815 987 1930 2109
Size: 783 32 172 943 179
Fragments arranged by size:
943 783 179 172 32
SmlI C'TyrA_G
Cuts at: 0 511 1693 1705 2109
Size: 511 1182 12 404
Fragments arranged by size:
1182 511 404 12
StuI AGG'CCT
Cuts at: 0 313 961 1901 2109
Size: 313 648 940 208
Fragments arranged by size:
940 648 313 208
StyI C'CwwG_G
Cuts at: 0 89 192 224 578 906 951 1313 1352
Size: 89 103 32 354 328 45 362 39
Cuts at: 1352 1410 2109
Size: 58 699
Fragments arranged by size:
699 362 354 328 103 89 58 45
39 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 631 759 1585 1629 2109
Size: 631 128 826 44 480
Fragments arranged by size:
826 631 480 128 44
TatI w'GTAC_w
Cuts at: 0 98 2109
Size: 98 2011
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1199 1231 1441 1473 1740 1772 2109
Size: 1199 32 210 32 267 32 337
Fragments arranged by size:
1199 337 267 210 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 637 2053 2109
Size: 637 1416 56
Fragments arranged by size:
1416 637 56
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 2109
Size: 525 1584
XhoI C'TCGA_G
Cuts at: 0 511 2109
Size: 511 1598
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AleI AlfI AloI AlwNI
ApaI AvaI BanI BanII BbvCI BciVI BglI BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KpnI MscI
MslI MspA1I NaeI NcoI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI
PvuII SacI SanDI SfcI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BcgI BclI BfrBI BglII BmgBI BsaBI BsaHI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BstBI BstZ17I ClaI CspCI DraI DrdI EarI
EciI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KasI MfeI MluI MmeI NarI NdeI NotI
NruI NsiI PacI PmeI PpiI PshAI PsiI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XmaI XmnI ZraI