Restriction map for
pFN21AE3506
(Linear) (Six-base) MAPSORT of: KIEE3506.seq Check: 2084 from: 1 to: 7860
KIEE3506.orf
With 182 enzymes: SgfI *
July 3, 2012 17:59 ..
AatII G_ACGT'C
Cuts at: 0 6821 7860
Size: 6821 1039
AccI GT'mk_AC
Cuts at: 0 2902 3508 3680 3717 6398 7860
Size: 2902 606 172 37 2681 1462
Fragments arranged by size:
2902 2681 1462 606 172 37
Acc65I G'GTAC_C
Cuts at: 0 1896 3862 7860
Size: 1896 1966 3998
Fragments arranged by size:
3998 1966 1896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 3498 4556 5801 6584 6668 7196 7860
Size: 563 2935 1058 1245 783 84 528 664
Fragments arranged by size:
2935 1245 1058 783 664 563 528 84
AfeI AGC'GCT
Cuts at: 0 2797 7860
Size: 2797 5063
AflII C'TTAA_G
Cuts at: 0 2073 7860
Size: 2073 5787
AflIII A'CryG_T
Cuts at: 0 617 7691 7860
Size: 617 7074 169
Fragments arranged by size:
7074 617 169
AhdI GACnn_n'nnGTC
Cuts at: 0 5501 6273 7860
Size: 5501 772 1587
Fragments arranged by size:
5501 1587 772
AleI CACnn'nnGTG
Cuts at: 0 6076 7860
Size: 6076 1784
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 7860
Size: 94 7766
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4163 4195 7860
Size: 4163 32 3665
Fragments arranged by size:
4163 3665 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1501 2053 2707 3587 4272 4509 5118 6786
Size: 1501 552 654 880 685 237 609 1668
Cuts at: 6786 7860
Size: 1074
Fragments arranged by size:
1668 1501 1074 880 685 654 609 552
237
ApaI G_GGCC'C
Cuts at: 0 197 208 7860
Size: 197 11 7652
Fragments arranged by size:
7652 197 11
ApoI r'AATT_y
Cuts at: 0 364 378 596 608 1212 1430 1507 1992
Size: 364 14 218 12 604 218 77 485
Cuts at: 1992 2137 2324 2602 4094 5259 5326 5387 5516
Size: 145 187 278 1492 1165 67 61 129
Cuts at: 5516 5962 5986 6224 7006 7414 7501 7557 7860
Size: 446 24 238 782 408 87 56 303
Fragments arranged by size:
1492 1165 782 604 485 446 408 364
303 278 238 218 218 187 145 129
87 77 67 61 56 24 14 12
AseI AT'TA_AT
Cuts at: 0 631 2555 7860
Size: 631 1924 5305
Fragments arranged by size:
5305 1924 631
AvaI C'yCGr_G
Cuts at: 0 190 3498 3944 7860
Size: 190 3308 446 3916
Fragments arranged by size:
3916 3308 446 190
AvrII C'CTAG_G
Cuts at: 0 6948 7860
Size: 6948 912
BamHI G'GATC_C
Cuts at: 0 5824 7860
Size: 5824 2036
BanI G'GyrC_C
Cuts at: 0 1896 2854 3862 7860
Size: 1896 958 1008 3998
Fragments arranged by size:
3998 1896 1008 958
BanII G_rGCy'C
Cuts at: 0 164 197 208 1091 2433 5138 7860
Size: 164 33 11 883 1342 2705 2722
Fragments arranged by size:
2722 2705 1342 883 164 33 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 3103 3779 3902 5100 7860
Size: 3103 676 123 1198 2760
Fragments arranged by size:
3103 2760 1198 676 123
BbvCI CC'TCA_GC
Cuts at: 0 6188 7860
Size: 6188 1672
BciVI GTATCCnnnnn_n'
Cuts at: 0 4206 7860
Size: 4206 3654
BclI T'GATC_A
Cuts at: 0 411 2406 2804 3354 4365 5346 5421 6054
Size: 411 1995 398 550 1011 981 75 633
Cuts at: 6054 6129 6935 7860
Size: 75 806 925
Fragments arranged by size:
1995 1011 981 925 806 633 550 411
398 75 75
BfrBI ATG'CAT
Cuts at: 0 1747 4986 5320 7860
Size: 1747 3239 334 2540
Fragments arranged by size:
3239 2540 1747 334
BglI GCCn_nnn'nGGC
Cuts at: 0 212 6255 7860
Size: 212 6043 1605
Fragments arranged by size:
6043 1605 212
BglII A'GATC_T
Cuts at: 0 2198 2749 5429 6900 7860
Size: 2198 551 2680 1471 960
Fragments arranged by size:
2680 2198 1471 960 551
BlpI GC'TnA_GC
Cuts at: 0 4457 7860
Size: 4457 3403
Bme1580I G_kGCm'C
Cuts at: 0 197 208 4706 7860
Size: 197 11 4498 3154
Fragments arranged by size:
4498 3154 197 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 1000 1688 1875 3073 5029 5682 6136 7860
Size: 1000 688 187 1198 1956 653 454 1724
Fragments arranged by size:
1956 1724 1198 1000 688 653 454 187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6213 7860
Size: 6213 1647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1835 2087 3135 4820 6031 6700 6745 6808
Size: 1835 252 1048 1685 1211 669 45 63
Cuts at: 6808 6917 7590 7860
Size: 109 673 270
Fragments arranged by size:
1835 1685 1211 1048 673 669 270 252
109 63 45
Bpu10I CC'TnA_GC
Cuts at: 0 348 1085 6188 7538 7685 7860
Size: 348 737 5103 1350 147 175
Fragments arranged by size:
5103 1350 737 348 175 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2308 5350 6358 7107 7860
Size: 2308 3042 1008 749 753
Fragments arranged by size:
3042 2308 1008 753 749
BsaI GGTCTCn'nnnn_
Cuts at: 0 685 1627 4478 4663 5375 5998 7860
Size: 685 942 2851 185 712 623 1862
Fragments arranged by size:
2851 1862 942 712 685 623 185
BsaAI yAC'GTr
Cuts at: 0 4247 7860
Size: 4247 3613
BsaBI GATnn'nnATC
Cuts at: 0 7853 7860
Size: 7853 7
BsaHI Gr'CG_yC
Cuts at: 0 6818 7860
Size: 6818 1042
BsaWI w'CCGG_w
Cuts at: 0 265 1128 2957 7860
Size: 265 863 1829 4903
Fragments arranged by size:
4903 1829 863 265
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4139 4169 5120 5150 7860
Size: 4139 30 951 30 2710
Fragments arranged by size:
4139 2710 951 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 28 1735 1788 4508 5555 6044 7162
Size: 25 3 1707 53 2720 1047 489 1118
Cuts at: 7162 7860
Size: 698
Fragments arranged by size:
2720 1707 1118 1047 698 489 53 25
3
BseYI C'CCAG_C
Cuts at: 0 184 1556 4599 7574 7860
Size: 184 1372 3043 2975 286
Fragments arranged by size:
3043 2975 1372 286 184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 4435 5224 7603 7860
Size: 983 3452 789 2379 257
Fragments arranged by size:
3452 2379 983 789 257
BsiEI CG_ry'CG
Cuts at: 0 136 7860
Size: 136 7724
BsiHKAI G_wGCw'C
Cuts at: 0 265 1091 1727 2433 2986 3524 6846 7860
Size: 265 826 636 706 553 538 3322 1014
Fragments arranged by size:
3322 1014 826 706 636 553 538 265
BsmI GAATG_Cn'
Cuts at: 0 1278 5106 6025 6102 6450 6915 7047 7860
Size: 1278 3828 919 77 348 465 132 813
Fragments arranged by size:
3828 1278 919 813 465 348 132 77
Bsp1286I G_dGCh'C
Cuts at: 0 164 197 208 265 1091 1727 2433 2986
Size: 164 33 11 57 826 636 706 553
Cuts at: 2986 3524 4706 5138 6846 7860
Size: 538 1182 432 1708 1014
Fragments arranged by size:
1708 1182 1014 826 706 636 553 538
432 164 57 33 11
BspHI T'CATG_A
Cuts at: 0 600 4001 7338 7368 7860
Size: 600 3401 3337 30 492
Fragments arranged by size:
3401 3337 600 492 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 5036 7860
Size: 5036 2824
BsrBI CCG'CTC
Cuts at: 0 132 7860
Size: 132 7728
BsrDI GCAATG_nn'
Cuts at: 0 2606 3385 6589 6693 7112 7860
Size: 2606 779 3204 104 419 748
Fragments arranged by size:
3204 2606 779 748 419 104
BsrFI r'CCGG_y
Cuts at: 0 2099 3830 6269 7860
Size: 2099 1731 2439 1591
Fragments arranged by size:
2439 2099 1731 1591
BsrGI T'GTAC_A
Cuts at: 0 4373 6873 7860
Size: 4373 2500 987
Fragments arranged by size:
4373 2500 987
BssSI C'ACGA_G
Cuts at: 0 304 7001 7860
Size: 304 6697 859
Fragments arranged by size:
6697 859 304
BstBI TT'CG_AA
Cuts at: 0 4098 7860
Size: 4098 3762
BstXI CCAn_nnnn'nTGG
Cuts at: 0 6723 7860
Size: 6723 1137
BstYI r'GATC_y
Cuts at: 0 663 1131 2198 2749 5429 5824 6192 6900
Size: 663 468 1067 551 2680 395 368 708
Cuts at: 6900 7389 7860
Size: 489 471
Fragments arranged by size:
2680 1067 708 663 551 489 471 468
395 368
BstZ17I GTA'TAC
Cuts at: 0 6399 7860
Size: 6399 1461
Bsu36I CC'TnA_GG
Cuts at: 0 951 7860
Size: 951 6909
BtgI C'CryG_G
Cuts at: 0 110 164 208 252 983 2489 3191 7860
Size: 110 54 44 44 731 1506 702 4669
Fragments arranged by size:
4669 1506 731 702 110 54 44 44
BtsI GCAGTG_nn'
Cuts at: 0 115 1760 3114 3171 4851 7591 7860
Size: 115 1645 1354 57 1680 2740 269
Fragments arranged by size:
2740 1680 1645 1354 269 115 57
ClaI AT'CG_AT
Cuts at: 0 2412 7860
Size: 2412 5448
DraI TTT'AAA
Cuts at: 0 552 732 2376 4823 7860
Size: 552 180 1644 2447 3037
Fragments arranged by size:
3037 2447 1644 552 180
DraIII CAC_nnn'GTG
Cuts at: 0 309 2653 4339 4738 7860
Size: 309 2344 1686 399 3122
Fragments arranged by size:
3122 2344 1686 399 309
DrdI GACnn_nn'nnGTC
Cuts at: 0 6817 7860
Size: 6817 1043
EaeI y'GGCC_r
Cuts at: 0 86 133 2057 2097 2220 3578 6713 7860
Size: 86 47 1924 40 123 1358 3135 1147
Fragments arranged by size:
3135 1924 1358 1147 123 86 47 40
EagI C'GGCC_G
Cuts at: 0 133 7860
Size: 133 7727
EarI CTCTTCn'nnn_
Cuts at: 0 1040 1546 2317 3272 4241 7860
Size: 1040 506 771 955 969 3619
Fragments arranged by size:
3619 1040 969 955 771 506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 236 7860
Size: 236 7624
EcoICRI GAG'CTC
Cuts at: 0 1089 2431 7860
Size: 1089 1342 5429
Fragments arranged by size:
5429 1342 1089
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1835 2087 3135 3498 4556 4820 5801
Size: 563 1272 252 1048 363 1058 264 981
Cuts at: 5801 6031 6584 6668 6700 6745 6808 6917 7196
Size: 230 553 84 32 45 63 109 279
Cuts at: 7196 7590 7860
Size: 394 270
Fragments arranged by size:
1272 1058 1048 981 563 553 394 363
279 270 264 252 230 109 84 63
45 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 7144 7195 7860
Size: 7144 51 665
Fragments arranged by size:
7144 665 51
EcoO109I rG'GnC_Cy
Cuts at: 0 75 204 3113 5612 7860
Size: 75 129 2909 2499 2248
Fragments arranged by size:
2909 2499 2248 129 75
EcoRI G'AATT_C
Cuts at: 0 364 1212 4094 5326 7557 7860
Size: 364 848 2882 1232 2231 303
Fragments arranged by size:
2882 2231 1232 848 364 303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4993 6510 6610 6690 7563 7860
Size: 4993 1517 100 80 873 297
Fragments arranged by size:
4993 1517 873 297 100 80
FspI TGC'GCA
Cuts at: 0 1177 7860
Size: 1177 6683
HaeII r_GCGC'y
Cuts at: 0 2799 7860
Size: 2799 5061
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 403 435 1078 1110 1200 1232 2019 2051
Size: 403 32 643 32 90 32 787 32
Cuts at: 2051 2572 2604 2809 2841 3650 3682 4216 4248
Size: 521 32 205 32 809 32 534 32
Cuts at: 4248 5032 5064 5487 5519 6121 6153 6259 6291
Size: 784 32 423 32 602 32 106 32
Cuts at: 6291 7045 7077 7860
Size: 754 32 783
Fragments arranged by size:
809 787 784 783 754 643 602 534
521 423 403 205 106 90 32 32
32 32 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 4861 7860
Size: 4861 2999
HindIII A'AGCT_T
Cuts at: 0 1588 3307 5468 7860
Size: 1588 1719 2161 2392
Fragments arranged by size:
2392 2161 1719 1588
KpnI G_GTAC'C
Cuts at: 0 1900 3866 7860
Size: 1900 1966 3994
Fragments arranged by size:
3994 1966 1900
MfeI C'AATT_G
Cuts at: 0 1142 4125 7860
Size: 1142 2983 3735
Fragments arranged by size:
3735 2983 1142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1149 1708 3723 4501 6788 7383 7733 7860
Size: 1149 559 2015 778 2287 595 350 127
Fragments arranged by size:
2287 2015 1149 778 595 559 350 127
MscI TGG'CCA
Cuts at: 0 88 2222 3580 6715 7860
Size: 88 2134 1358 3135 1145
Fragments arranged by size:
3135 2134 1358 1145 88
MslI CAynn'nnrTG
Cuts at: 0 622 1199 2936 4409 5453 6076 7052 7249
Size: 622 577 1737 1473 1044 623 976 197
Cuts at: 7249 7860
Size: 611
Fragments arranged by size:
1737 1473 1044 976 623 622 611 577
197
MspA1I CmG'CkG
Cuts at: 0 173 210 254 1498 2050 3565 4371 4461
Size: 173 37 44 1244 552 1515 806 90
Cuts at: 4461 4660 4840 5118 7860
Size: 199 180 278 2742
Fragments arranged by size:
2742 1515 1244 806 552 278 199 180
173 90 44 37
NaeI GCC'GGC
Cuts at: 0 3832 7860
Size: 3832 4028
NcoI C'CATG_G
Cuts at: 0 164 2489 3191 7860
Size: 164 2325 702 4669
Fragments arranged by size:
4669 2325 702 164
NdeI CA'TA_TG
Cuts at: 0 1749 2343 3186 5322 7860
Size: 1749 594 843 2136 2538
Fragments arranged by size:
2538 2136 1749 843 594
NgoMIV G'CCGG_C
Cuts at: 0 3830 7860
Size: 3830 4030
NsiI A_TGCA'T
Cuts at: 0 1749 4988 5322 7860
Size: 1749 3239 334 2538
Fragments arranged by size:
3239 2538 1749 334
NspI r_CATG'y
Cuts at: 0 621 1119 1182 2674 3255 4168 7860
Size: 621 498 63 1492 581 913 3692
Fragments arranged by size:
3692 1492 913 621 581 498 63
PciI A'CATG_T
Cuts at: 0 617 7860
Size: 617 7243
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 3063 4272 7860
Size: 309 2754 1209 3588
Fragments arranged by size:
3588 2754 1209 309
PfoI T'CCnGG_A
Cuts at: 0 1025 7860
Size: 1025 6835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5531 5563 7860
Size: 5531 32 2297
Fragments arranged by size:
5531 2297 32
PpuMI rG'GwC_Cy
Cuts at: 0 3113 5612 7860
Size: 3113 2499 2248
Fragments arranged by size:
3113 2499 2248
PshAI GACnn'nnGTC
Cuts at: 0 3849 3891 4188 7478 7860
Size: 3849 42 297 3290 382
Fragments arranged by size:
3849 3290 382 297 42
PsiI TTA'TAA
Cuts at: 0 4966 7860
Size: 4966 2894
PspOMI G'GGCC_C
Cuts at: 0 193 204 7860
Size: 193 11 7656
Fragments arranged by size:
7656 193 11
PspXI vC'TCGA_Gb
Cuts at: 0 3944 7860
Size: 3944 3916
PstI C_TGCA'G
Cuts at: 0 1497 4029 5243 7536 7860
Size: 1497 2532 1214 2293 324
Fragments arranged by size:
2532 2293 1497 1214 324
PvuII CAG'CTG
Cuts at: 0 2050 3565 4371 4461 4660 4840 5118 7860
Size: 2050 1515 806 90 199 180 278 2742
Fragments arranged by size:
2742 2050 1515 806 278 199 180 90
SacI G_AGCT'C
Cuts at: 0 1091 2433 7860
Size: 1091 1342 5427
Fragments arranged by size:
5427 1342 1091
SacII CC_GC'GG
Cuts at: 0 211 255 7860
Size: 211 44 7605
Fragments arranged by size:
7605 211 44
SanDI GG'GwC_CC
Cuts at: 0 3113 7860
Size: 3113 4747
SapI GCTCTTCn'nnn_
Cuts at: 0 1546 2317 3272 7860
Size: 1546 771 955 4588
Fragments arranged by size:
4588 1546 955 771
ScaI AGT'ACT
Cuts at: 0 5245 6850 7546 7860
Size: 5245 1605 696 314
Fragments arranged by size:
5245 1605 696 314
SfcI C'TryA_G
Cuts at: 0 1277 1493 2544 2720 2810 3718 4025 4252
Size: 1277 216 1051 176 90 908 307 227
Cuts at: 4252 5239 7532 7591 7792 7860
Size: 987 2293 59 201 68
Fragments arranged by size:
2293 1277 1051 987 908 307 227 216
201 176 90 68 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 6255 7860
Size: 6255 1605
SmaI CCC'GGG
Cuts at: 0 192 3500 7860
Size: 192 3308 4360
Fragments arranged by size:
4360 3308 192
SmlI C'TyrA_G
Cuts at: 0 2073 2287 3944 5365 6373 7122 7860
Size: 2073 214 1657 1421 1008 749 738
Fragments arranged by size:
2073 1657 1421 1008 749 738 214
SpeI A'CTAG_T
Cuts at: 0 2088 4480 5335 7860
Size: 2088 2392 855 2525
Fragments arranged by size:
2525 2392 2088 855
SphI G_CATG'C
Cuts at: 0 1119 1182 4168 7860
Size: 1119 63 2986 3692
Fragments arranged by size:
3692 2986 1119 63
SspI AAT'ATT
Cuts at: 0 6634 7376 7860
Size: 6634 742 484
Fragments arranged by size:
6634 742 484
StuI AGG'CCT
Cuts at: 0 1714 1812 7860
Size: 1714 98 6048
Fragments arranged by size:
6048 1714 98
StyI C'CwwG_G
Cuts at: 0 164 1715 2192 2489 3191 3283 5012 6948
Size: 164 1551 477 297 702 92 1729 1936
Cuts at: 6948 7860
Size: 912
Fragments arranged by size:
1936 1729 1551 912 702 477 297 164
92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 676 1881 7119 7860
Size: 676 1205 5238 741
Fragments arranged by size:
5238 1205 741 676
TatI w'GTAC_w
Cuts at: 0 4373 4885 5243 6848 6873 7259 7544 7860
Size: 4373 512 358 1605 25 386 285 316
Fragments arranged by size:
4373 1605 512 386 358 316 285 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 827 859 5160 5192 5899 5931 6308 6340
Size: 827 32 4301 32 707 32 377 32
Cuts at: 6340 7860
Size: 1520
Fragments arranged by size:
4301 1520 827 707 377 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 488 1006 7860
Size: 488 518 6854
Fragments arranged by size:
6854 518 488
XbaI T'CTAG_A
Cuts at: 0 4810 5550 7860
Size: 4810 740 2310
Fragments arranged by size:
4810 2310 740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 681 7860
Size: 681 7179
XhoI C'TCGA_G
Cuts at: 0 3944 7860
Size: 3944 3916
XmaI C'CCGG_G
Cuts at: 0 190 3498 7860
Size: 190 3308 4362
Fragments arranged by size:
4362 3308 190
XmnI GAAnn'nnTTC
Cuts at: 0 2525 5272 7354 7677 7860
Size: 2525 2747 2082 323 183
Fragments arranged by size:
2747 2525 2082 323 183
ZraI GAC'GTC
Cuts at: 0 6819 7860
Size: 6819 1041
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApoI AseI AvaI
AvrII BamHI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PfoI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SacI
SacII SanDI SapI ScaI SfcI SfiI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AgeI ApaLI AscI AsiSI BaeI BbeI
BcgI BmgBI BmtI BsiWI BsmBI BspEI BssHII BstAPI
BstEII BtgZI CspCI EcoRV FseI FspAI HpaI KasI
MluI NarI NheI NotI NruI PacI PasI PmeI
PmlI PsrI PvuI RsrII SalI SbfI SexAI SfoI
SgfI SgrAI SnaBI SrfI SwaI