Restriction map for
pFN21AE3502
(Linear) (Six-base) MAPSORT of: KIEE3502.seq Check: 153 from: 1 to: 4746
KIEE3502.orf
With 182 enzymes: SgfI *
July 3, 2012 17:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 59 4746
Size: 59 4687
AccI GT'mk_AC
Cuts at: 0 2651 4746
Size: 2651 2095
Acc65I G'GTAC_C
Cuts at: 0 1545 1575 4746
Size: 1545 30 3171
Fragments arranged by size:
3171 1545 30
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 587 773 1201 1868 2315 2762 3569
Size: 469 118 186 428 667 447 447 807
Cuts at: 3569 3916 3933 4106 4213 4263 4454 4621 4746
Size: 347 17 173 107 50 191 167 125
Fragments arranged by size:
807 667 469 447 447 428 347 191
186 173 167 125 118 107 50 17
AflIII A'CryG_T
Cuts at: 0 960 3605 4097 4746
Size: 960 2645 492 649
Fragments arranged by size:
2645 960 649 492
AgeI A'CCGG_T
Cuts at: 0 4157 4746
Size: 4157 589
AhdI GACnn_n'nnGTC
Cuts at: 0 3260 4098 4746
Size: 3260 838 648
Fragments arranged by size:
3260 838 648
AleI CACnn'nnGTG
Cuts at: 0 3950 4746
Size: 3950 796
AlwNI CAG_nnn'CTG
Cuts at: 0 1263 1717 3358 3373 4084 4318 4746
Size: 1263 454 1641 15 711 234 428
Fragments arranged by size:
1641 1263 711 454 428 234 15
ApaLI G'TGCA_C
Cuts at: 0 1271 4635 4746
Size: 1271 3364 111
Fragments arranged by size:
3364 1271 111
ApoI r'AATT_y
Cuts at: 0 888 1555 4746
Size: 888 667 3191
Fragments arranged by size:
3191 888 667
AvaI C'yCGr_G
Cuts at: 0 1328 4640 4746
Size: 1328 3312 106
Fragments arranged by size:
3312 1328 106
AvrII C'CTAG_G
Cuts at: 0 1462 4746
Size: 1462 3284
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1805 1838 4746
Size: 1805 33 2908
Fragments arranged by size:
2908 1805 33
BamHI G'GATC_C
Cuts at: 0 57 4746
Size: 57 4689
BanI G'GyrC_C
Cuts at: 0 365 657 1302 1398 1458 1545 1575 1632
Size: 365 292 645 96 60 87 30 57
Cuts at: 1632 1662 1947 2177 2721 2958 4023 4746
Size: 30 285 230 544 237 1065 723
Fragments arranged by size:
1065 723 645 544 365 292 285 237
230 96 87 60 57 30 30
BanII G_rGCy'C
Cuts at: 0 762 3222 4181 4727 4746
Size: 762 2460 959 546 19
Fragments arranged by size:
2460 959 762 546 19
BbeI G_GCGC'C
Cuts at: 0 2181 2962 4746
Size: 2181 781 1784
Fragments arranged by size:
2181 1784 781
BbsI GAAGACnn'nnnn_
Cuts at: 0 142 3747 3904 4746
Size: 142 3605 157 842
Fragments arranged by size:
3605 842 157 142
BbvCI CC'TCA_GC
Cuts at: 0 2423 4746
Size: 2423 2323
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 386 420 4746
Size: 386 34 4326
Fragments arranged by size:
4326 386 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1500 1806 4746
Size: 1500 306 2940
Fragments arranged by size:
2940 1500 306
BclI T'GATC_A
Cuts at: 0 2583 2838 2877 3375 4068 4218 4746
Size: 2583 255 39 498 693 150 528
Fragments arranged by size:
2583 693 528 498 255 150 39
BfrBI ATG'CAT
Cuts at: 0 4018 4064 4746
Size: 4018 46 682
Fragments arranged by size:
4018 682 46
BglI GCCn_nnn'nGGC
Cuts at: 0 1179 4746
Size: 1179 3567
BglII A'GATC_T
Cuts at: 0 2359 3632 4746
Size: 2359 1273 1114
Fragments arranged by size:
2359 1273 1114
BlpI GC'TnA_GC
Cuts at: 0 492 4284 4746
Size: 492 3792 462
Fragments arranged by size:
3792 492 462
Bme1580I G_kGCm'C
Cuts at: 0 600 1275 1461 1950 4026 4639 4746
Size: 600 675 186 489 2076 613 107
Fragments arranged by size:
2076 675 613 600 489 186 107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1669 4746
Size: 39 1630 3077
Fragments arranged by size:
3077 1630 39
Bpu10I CC'TnA_GC
Cuts at: 0 1277 1298 1352 2423 3536 4746
Size: 1277 21 54 1071 1113 1210
Fragments arranged by size:
1277 1210 1113 1071 54 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4183 4746
Size: 4183 563
BsaI GGTCTCn'nnnn_
Cuts at: 0 2124 2435 2558 3270 4161 4746
Size: 2124 311 123 712 891 585
Fragments arranged by size:
2124 891 712 585 311 123
BsaBI GATnn'nnATC
Cuts at: 0 3790 4295 4746
Size: 3790 505 451
Fragments arranged by size:
3790 505 451
BsaHI Gr'CG_yC
Cuts at: 0 2178 2959 4746
Size: 2178 781 1787
Fragments arranged by size:
2178 1787 781
BsaWI w'CCGG_w
Cuts at: 0 2699 4157 4746
Size: 2699 1458 589
Fragments arranged by size:
2699 1458 589
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 234 264 4746
Size: 234 30 4482
Fragments arranged by size:
4482 234 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 755 3325 4746
Size: 755 2570 1421
Fragments arranged by size:
2570 1421 755
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1688 3113 3344 4746
Size: 416 1272 1425 231 1402
Fragments arranged by size:
1425 1402 1272 416 231
BsiHKAI G_wGCw'C
Cuts at: 0 1275 1433 1826 4181 4639 4727 4746
Size: 1275 158 393 2355 458 88 19
Fragments arranged by size:
2355 1275 458 393 158 88 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3290 4746
Size: 3290 1456
Bsp1286I G_dGCh'C
Cuts at: 0 600 762 1275 1433 1461 1826 1950 3222
Size: 600 162 513 158 28 365 124 1272
Cuts at: 3222 4026 4181 4639 4727 4746
Size: 804 155 458 88 19
Fragments arranged by size:
1272 804 600 513 458 365 162 158
155 124 88 28 19
BspEI T'CCGG_A
Cuts at: 0 2699 4746
Size: 2699 2047
BspHI T'CATG_A
Cuts at: 0 129 3378 4700 4746
Size: 129 3249 1322 46
Fragments arranged by size:
3249 1322 129 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 391 1464 3288 4598 4746
Size: 59 332 1073 1824 1310 148
Fragments arranged by size:
1824 1310 1073 332 148 59
BsrDI GCAATG_nn'
Cuts at: 0 2859 4746
Size: 2859 1887
BsrFI r'CCGG_y
Cuts at: 0 4157 4746
Size: 4157 589
BsrGI T'GTAC_A
Cuts at: 0 4522 4746
Size: 4522 224
BssSI C'ACGA_G
Cuts at: 0 1102 4746
Size: 1102 3644
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1133 4746
Size: 1133 3613
BstBI TT'CG_AA
Cuts at: 0 3198 4746
Size: 3198 1548
BstXI CCAn_nnnn'nTGG
Cuts at: 0 747 4587 4746
Size: 747 3840 159
Fragments arranged by size:
3840 747 159
BstYI r'GATC_y
Cuts at: 0 39 57 264 2359 3632 4475 4746
Size: 39 18 207 2095 1273 843 271
Fragments arranged by size:
2095 1273 843 271 207 39 18
BstZ17I GTA'TAC
Cuts at: 0 2652 4746
Size: 2652 2094
Bsu36I CC'TnA_GG
Cuts at: 0 662 1859 3524 4746
Size: 662 1197 1665 1222
Fragments arranged by size:
1665 1222 1197 662
BtgI C'CryG_G
Cuts at: 0 3081 4746
Size: 3081 1665
BtsI GCAGTG_nn'
Cuts at: 0 1252 3159 3174 4046 4746
Size: 1252 1907 15 872 700
Fragments arranged by size:
1907 1252 872 700 15
ClaI AT'CG_AT
Cuts at: 0 4307 4746
Size: 4307 439
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1517 1552 1790 1825 3761 3796 4746
Size: 1517 35 238 35 1936 35 950
Fragments arranged by size:
1936 1517 950 238 35 35 35
DraI TTT'AAA
Cuts at: 0 446 2625 4746
Size: 446 2179 2121
Fragments arranged by size:
2179 2121 446
DraIII CAC_nnn'GTG
Cuts at: 0 1791 4746
Size: 1791 2955
DrdI GACnn_nn'nnGTC
Cuts at: 0 31 4746
Size: 31 4715
EarI CTCTTCn'nnn_
Cuts at: 0 254 802 1190 2415 2738 3156 3203 3268
Size: 254 548 388 1225 323 418 47 65
Cuts at: 3268 4471 4715 4746
Size: 1203 244 31
Fragments arranged by size:
1225 1203 548 418 388 323 254 244
65 47 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3739 4746
Size: 3739 1007
EcoICRI GAG'CTC
Cuts at: 0 4179 4725 4746
Size: 4179 546 21
Fragments arranged by size:
4179 546 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 469 587 773 1201 1669 1868 2315
Size: 39 430 118 186 428 468 199 447
Cuts at: 2315 2762 3569 3916 3933 4106 4213 4263 4454
Size: 447 807 347 17 173 107 50 191
Cuts at: 4454 4621 4746
Size: 167 125
Fragments arranged by size:
807 468 447 447 430 428 347 199
191 186 173 167 125 118 107 50
39 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 194 3452 3837 4746
Size: 194 3258 385 909
Fragments arranged by size:
3258 909 385 194
EcoO109I rG'GnC_Cy
Cuts at: 0 7 684 1087 1602 4746
Size: 7 677 403 515 3144
Fragments arranged by size:
3144 677 515 403 7
EcoRV GAT'ATC
Cuts at: 0 2199 4297 4746
Size: 2199 2098 449
Fragments arranged by size:
2199 2098 449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4293 4674 4746
Size: 4293 381 72
Fragments arranged by size:
4293 381 72
FspI TGC'GCA
Cuts at: 0 4675 4746
Size: 4675 71
HaeII r_GCGC'y
Cuts at: 0 2181 2962 4746
Size: 2181 781 1784
Fragments arranged by size:
2181 1784 781
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 156 188 420 452 706 738 1912 1944
Size: 156 32 232 32 254 32 1174 32
Cuts at: 1944 1986 2018 2038 2070 2242 2274 2794 2826
Size: 42 32 20 32 172 32 520 32
Cuts at: 2826 3246 3278 4084 4116 4286 4300 4312 4318
Size: 420 32 806 32 170 14 12 6
Cuts at: 4318 4332 4344 4486 4518 4746
Size: 14 12 142 32 228
Fragments arranged by size:
1174 806 520 420 254 232 228 172
170 156 142 42 32 32 32 32
32 32 32 32 32 32 32 20
14 14 12 12 6
HincII GTy'rAC
Cuts at: 0 2601 2714 3561 3932 4239 4746
Size: 2601 113 847 371 307 507
Fragments arranged by size:
2601 847 507 371 307 113
HindIII A'AGCT_T
Cuts at: 0 2140 4746
Size: 2140 2606
HpaI GTT'AAC
Cuts at: 0 2714 3561 3932 4746
Size: 2714 847 371 814
Fragments arranged by size:
2714 847 814 371
KasI G'GCGC_C
Cuts at: 0 2177 2958 4746
Size: 2177 781 1788
Fragments arranged by size:
2177 1788 781
KpnI G_GTAC'C
Cuts at: 0 1549 1579 4746
Size: 1549 30 3167
Fragments arranged by size:
3167 1549 30
MfeI C'AATT_G
Cuts at: 0 1895 3986 4746
Size: 1895 2091 760
Fragments arranged by size:
2091 1895 760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 3984 4234 4746
Size: 85 3899 250 512
Fragments arranged by size:
3899 512 250 85
MslI CAynn'nnrTG
Cuts at: 0 745 2846 2909 3383 3608 3950 4013 4561
Size: 745 2101 63 474 225 342 63 548
Cuts at: 4561 4746
Size: 185
Fragments arranged by size:
2101 745 548 474 342 225 185 63
63
MspA1I CmG'CkG
Cuts at: 0 154 363 839 2427 4144 4746
Size: 154 209 476 1588 1717 602
Fragments arranged by size:
1717 1588 602 476 209 154
NarI GG'CG_CC
Cuts at: 0 2178 2959 4746
Size: 2178 781 1787
Fragments arranged by size:
2178 1787 781
NcoI C'CATG_G
Cuts at: 0 3081 4746
Size: 3081 1665
NsiI A_TGCA'T
Cuts at: 0 4020 4066 4746
Size: 4020 46 680
Fragments arranged by size:
4020 680 46
NspI r_CATG'y
Cuts at: 0 921 964 3139 3587 3609 4101 4746
Size: 921 43 2175 448 22 492 645
Fragments arranged by size:
2175 921 645 492 448 43 22
PciI A'CATG_T
Cuts at: 0 960 3605 4097 4746
Size: 960 2645 492 649
Fragments arranged by size:
2645 960 649 492
PflMI CCAn_nnn'nTGG
Cuts at: 0 842 3322 4541 4746
Size: 842 2480 1219 205
Fragments arranged by size:
2480 1219 842 205
PfoI T'CCnGG_A
Cuts at: 0 3264 4746
Size: 3264 1482
PpuMI rG'GwC_Cy
Cuts at: 0 7 684 1087 4746
Size: 7 677 403 3659
Fragments arranged by size:
3659 677 403 7
PshAI GACnn'nnGTC
Cuts at: 0 3282 4746
Size: 3282 1464
PsiI TTA'TAA
Cuts at: 0 3733 4746
Size: 3733 1013
PstI C_TGCA'G
Cuts at: 0 1259 1475 4746
Size: 1259 216 3271
Fragments arranged by size:
3271 1259 216
PvuII CAG'CTG
Cuts at: 0 154 4144 4746
Size: 154 3990 602
Fragments arranged by size:
3990 602 154
SacI G_AGCT'C
Cuts at: 0 4181 4727 4746
Size: 4181 546 19
Fragments arranged by size:
4181 546 19
SapI GCTCTTCn'nnn_
Cuts at: 0 1190 4715 4746
Size: 1190 3525 31
Fragments arranged by size:
3525 1190 31
ScaI AGT'ACT
Cuts at: 0 298 4746
Size: 298 4448
SexAI A'CCwGG_T
Cuts at: 0 1541 1628 2870 3832 4746
Size: 1541 87 1242 962 914
Fragments arranged by size:
1541 1242 962 914 87
SfcI C'TryA_G
Cuts at: 0 1225 1255 1345 1441 1471 3045 3304 4508
Size: 1225 30 90 96 30 1574 259 1204
Cuts at: 4508 4746
Size: 238
Fragments arranged by size:
1574 1225 1204 259 238 96 90 30
30
SfoI GGC'GCC
Cuts at: 0 2179 2960 4746
Size: 2179 781 1786
Fragments arranged by size:
2179 1786 781
SmaI CCC'GGG
Cuts at: 0 4642 4746
Size: 4642 104
SmlI C'TyrA_G
Cuts at: 0 4198 4746
Size: 4198 548
SspI AAT'ATT
Cuts at: 0 71 4746
Size: 71 4675
StuI AGG'CCT
Cuts at: 0 3334 4746
Size: 3334 1412
StyI C'CwwG_G
Cuts at: 0 96 785 1174 1462 2971 3081 3222 4746
Size: 96 689 389 288 1509 110 141 1524
Fragments arranged by size:
1524 1509 689 389 288 141 110 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 227 396 944 4746
Size: 227 169 548 3802
Fragments arranged by size:
3802 548 227 169
TatI w'GTAC_w
Cuts at: 0 296 1064 4522 4746
Size: 296 768 3458 224
Fragments arranged by size:
3458 768 296 224
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 234 266 1133 1165 4746
Size: 191 32 11 32 867 32 3581
Fragments arranged by size:
3581 867 191 32 32 32 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2614 4746
Size: 2614 2132
XmaI C'CCGG_G
Cuts at: 0 4640 4746
Size: 4640 106
XmnI GAAnn'nnTTC
Cuts at: 0 482 631 2276 4746
Size: 482 149 1645 2470
Fragments arranged by size:
2470 1645 482 149
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtsI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MmeI MslI
MspA1I NarI NcoI NsiI NspI PciI PflMI PfoI
PpuMI PshAI PsiI PstI PvuII SacI SapI ScaI
SexAI SfcI SfoI SmaI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AlfI AloI ApaI AscI
AseI AsiSI BmgBI BmrI BmtI BplI BsaAI BseYI
BsiEI BsiWI BsmI BsrBI BssHII BstEII BtgZI EaeI
EagI EcoRI FseI FspAI MluI MscI NaeI NdeI
NgoMIV NheI NotI NruI PacI PasI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XhoI ZraI