Restriction map for
pFN21AE3492
(Linear) (Six-base) MAPSORT of: KIEE3492.seq Check: 9729 from: 1 to: 5529
KIEE3492.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4732 5529
Size: 4732 797
AatII G_ACGT'C
Cuts at: 0 4104 5529
Size: 4104 1425
AccI GT'mk_AC
Cuts at: 0 735 4914 5403 5529
Size: 735 4179 489 126
Fragments arranged by size:
4179 735 489 126
AclI AA'CG_TT
Cuts at: 0 70 2707 5529
Size: 70 2637 2822
Fragments arranged by size:
2822 2637 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1546 1901 3226 3241 3304 3497 3571
Size: 665 881 355 1325 15 63 193 74
Cuts at: 3571 3803 4279 4313 4542 5219 5529
Size: 232 476 34 229 677 310
Fragments arranged by size:
1325 881 677 665 476 355 310 232
229 193 74 63 34 15
AflII C'TTAA_G
Cuts at: 0 1707 2209 2893 5529
Size: 1707 502 684 2636
Fragments arranged by size:
2636 1707 684 502
AleI CACnn'nnGTG
Cuts at: 0 4781 5529
Size: 4781 748
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4637 4669 5529
Size: 4637 32 860
Fragments arranged by size:
4637 860 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3709 5437 5529
Size: 3709 1728 92
Fragments arranged by size:
3709 1728 92
ApoI r'AATT_y
Cuts at: 0 11 259 418 431 888 1018 1575 1641
Size: 11 248 159 13 457 130 557 66
Cuts at: 1641 1769 1819 1992 2085 2580 3330 3807 3862
Size: 128 50 173 93 495 750 477 55
Cuts at: 3862 3954 3989 4144 4446 4789 5493 5529
Size: 92 35 155 302 343 704 36
Fragments arranged by size:
750 704 557 495 477 457 343 302
248 173 159 155 130 128 93 92
66 55 50 36 35 13 11
AseI AT'TA_AT
Cuts at: 0 164 1222 3142 4000 4229 5529
Size: 164 1058 1920 858 229 1300
Fragments arranged by size:
1920 1300 1058 858 229 164
BanII G_rGCy'C
Cuts at: 0 2228 3058 5529
Size: 2228 830 2471
Fragments arranged by size:
2471 2228 830
BbsI GAAGACnn'nnnn_
Cuts at: 0 1058 1097 1552 1694 2755 3083 3389 3446
Size: 1058 39 455 142 1061 328 306 57
Cuts at: 3446 3602 3811 4610 5529
Size: 156 209 799 919
Fragments arranged by size:
1061 1058 919 799 455 328 306 209
156 142 57 39
BclI T'GATC_A
Cuts at: 0 290 3467 5529
Size: 290 3177 2062
Fragments arranged by size:
3177 2062 290
BlpI GC'TnA_GC
Cuts at: 0 1607 1807 2162 3937 5529
Size: 1607 200 355 1775 1592
Fragments arranged by size:
1775 1607 1592 355 200
BmrI ACTGGGnnnn_n'
Cuts at: 0 4870 5529
Size: 4870 659
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3077 5529
Size: 3077 2452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 695 4263 4867 4927 5529
Size: 49 646 3568 604 60 602
Fragments arranged by size:
3568 646 604 602 60 49
Bpu10I CC'TnA_GC
Cuts at: 0 787 5529
Size: 787 4742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 2815 3235 3592 5001 5231 5529
Size: 862 1953 420 357 1409 230 298
Fragments arranged by size:
1953 1409 862 420 357 298 230
BsaI GGTCTCn'nnnn_
Cuts at: 0 664 3581 5172 5529
Size: 664 2917 1591 357
Fragments arranged by size:
2917 1591 664 357
BsaAI yAC'GTr
Cuts at: 0 2722 5529
Size: 2722 2807
BsaBI GATnn'nnATC
Cuts at: 0 4229 5529
Size: 4229 1300
BsaHI Gr'CG_yC
Cuts at: 0 4101 5529
Size: 4101 1428
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3050 4151 5529
Size: 3050 1101 1378
Fragments arranged by size:
3050 1378 1101
BseYI C'CCAG_C
Cuts at: 0 1289 5529
Size: 1289 4240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 674 1751 4423 4766 5529
Size: 413 261 1077 2672 343 763
Fragments arranged by size:
2672 1077 763 413 343 261
BsiHKAI G_wGCw'C
Cuts at: 0 1116 2228 3058 4883 5529
Size: 1116 1112 830 1825 646
Fragments arranged by size:
1825 1116 1112 830 646
BsmI GAATG_Cn'
Cuts at: 0 230 3315 3378 5529
Size: 230 3085 63 2151
Fragments arranged by size:
3085 2151 230 63
Bsp1286I G_dGCh'C
Cuts at: 0 1116 2228 3058 4883 5529
Size: 1116 1112 830 1825 646
Fragments arranged by size:
1825 1116 1112 830 646
BspHI T'CATG_A
Cuts at: 0 38 293 1173 1776 2787 3362 3477 3498
Size: 38 255 880 603 1011 575 115 21
Cuts at: 3498 3696 4803 5529
Size: 198 1107 726
Fragments arranged by size:
1107 1011 880 726 603 575 255 198
115 38 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 524 4732 5495 5529
Size: 524 4208 763 34
Fragments arranged by size:
4208 763 524 34
BsrBI CCG'CTC
Cuts at: 0 651 4271 5529
Size: 651 3620 1258
Fragments arranged by size:
3620 1258 651
BsrDI GCAATG_nn'
Cuts at: 0 526 580 1470 2383 5529
Size: 526 54 890 913 3146
Fragments arranged by size:
3146 913 890 526 54
BssSI C'ACGA_G
Cuts at: 0 1109 5324 5529
Size: 1109 4215 205
Fragments arranged by size:
4215 1109 205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 466 5529
Size: 466 5063
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 4572 4783 5529
Size: 51 4521 211 746
Fragments arranged by size:
4521 746 211 51
BstYI r'GATC_y
Cuts at: 0 1587 4047 4561 5529
Size: 1587 2460 514 968
Fragments arranged by size:
2460 1587 968 514
BtgI C'CryG_G
Cuts at: 0 4409 5529
Size: 4409 1120
BtsI GCAGTG_nn'
Cuts at: 0 3169 4788 4833 5453 5529
Size: 3169 1619 45 620 76
Fragments arranged by size:
3169 1619 620 76 45
DraI TTT'AAA
Cuts at: 0 1952 2046 3773 5529
Size: 1952 94 1727 1756
Fragments arranged by size:
1952 1756 1727 94
DraIII CAC_nnn'GTG
Cuts at: 0 703 2722 4621 5529
Size: 703 2019 1899 908
Fragments arranged by size:
2019 1899 908 703
DrdI GACnn_nn'nnGTC
Cuts at: 0 540 5529
Size: 540 4989
EaeI y'GGCC_r
Cuts at: 0 83 4356 5108 5529
Size: 83 4273 752 421
Fragments arranged by size:
4273 752 421 83
EarI CTCTTCn'nnn_
Cuts at: 0 641 687 1424 3302 4115 4551 5070 5529
Size: 641 46 737 1878 813 436 519 459
Fragments arranged by size:
1878 813 737 641 519 459 436 46
EcoICRI GAG'CTC
Cuts at: 0 2226 3056 5529
Size: 2226 830 2473
Fragments arranged by size:
2473 2226 830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 665 695 1546 1901 3226 3241 3304
Size: 49 616 30 851 355 1325 15 63
Cuts at: 3304 3497 3571 3803 4263 4279 4313 4542 4867
Size: 193 74 232 460 16 34 229 325
Cuts at: 4867 4927 5219 5529
Size: 60 292 310
Fragments arranged by size:
1325 851 616 460 355 325 310 292
232 229 193 74 63 60 49 34
30 16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 5237 5529
Size: 5237 292
EcoRI G'AATT_C
Cuts at: 0 431 2085 5529
Size: 431 1654 3444
Fragments arranged by size:
3444 1654 431
EcoRV GAT'ATC
Cuts at: 0 1307 5529
Size: 1307 4222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1186 1627 2224 4309 5047 5529
Size: 1186 441 597 2085 738 482
Fragments arranged by size:
2085 1186 738 597 482 441
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 892 924 1357 1389 1421 2361 2393 3433
Size: 892 32 433 32 32 940 32 1040
Cuts at: 3433 3459 3465 3491 3508 3540 3889 3921 4579
Size: 26 6 26 17 32 349 32 658
Cuts at: 4579 4611 4804 4836 4902 4934 5169 5201 5529
Size: 32 193 32 66 32 235 32 328
Fragments arranged by size:
1040 940 892 658 433 349 328 235
193 66 32 32 32 32 32 32
32 32 32 32 26 26 17 6
HincII GTy'rAC
Cuts at: 0 736 1370 3766 4542 4915 5529
Size: 736 634 2396 776 373 614
Fragments arranged by size:
2396 776 736 634 614 373
HindIII A'AGCT_T
Cuts at: 0 970 3316 4990 5529
Size: 970 2346 1674 539
Fragments arranged by size:
2346 1674 970 539
MfeI C'AATT_G
Cuts at: 0 286 2497 2974 3892 5529
Size: 286 2211 477 918 1637
Fragments arranged by size:
2211 1637 918 477 286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1067 2547 3614 5529
Size: 803 264 1480 1067 1915
Fragments arranged by size:
1915 1480 1067 803 264
MscI TGG'CCA
Cuts at: 0 4358 5110 5529
Size: 4358 752 419
Fragments arranged by size:
4358 752 419
MslI CAynn'nnrTG
Cuts at: 0 244 2155 4781 4808 5529
Size: 244 1911 2626 27 721
Fragments arranged by size:
2626 1911 721 244 27
MspA1I CmG'CkG
Cuts at: 0 463 1716 2436 5434 5529
Size: 463 1253 720 2998 95
Fragments arranged by size:
2998 1253 720 463 95
NcoI C'CATG_G
Cuts at: 0 4409 5529
Size: 4409 1120
PasI CC'CwG_GG
Cuts at: 0 1347 5134 5529
Size: 1347 3787 395
Fragments arranged by size:
3787 1347 395
PflMI CCAn_nnn'nTGG
Cuts at: 0 3070 3894 4365 5529
Size: 3070 824 471 1164
Fragments arranged by size:
3070 1164 824 471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1098 1130 5529
Size: 1098 32 4399
Fragments arranged by size:
4399 1098 32
PshAI GACnn'nnGTC
Cuts at: 0 4610 5529
Size: 4610 919
PsiI TTA'TAA
Cuts at: 0 1025 1859 2966 5529
Size: 1025 834 1107 2563
Fragments arranged by size:
2563 1107 1025 834
PstI C_TGCA'G
Cuts at: 0 3714 4554 5460 5529
Size: 3714 840 906 69
Fragments arranged by size:
3714 906 840 69
PvuII CAG'CTG
Cuts at: 0 2436 5434 5529
Size: 2436 2998 95
Fragments arranged by size:
2998 2436 95
SacI G_AGCT'C
Cuts at: 0 2228 3058 5529
Size: 2228 830 2471
Fragments arranged by size:
2471 2228 830
SalI G'TCGA_C
Cuts at: 0 734 4913 5529
Size: 734 4179 616
Fragments arranged by size:
4179 734 616
SapI GCTCTTCn'nnn_
Cuts at: 0 641 1424 5529
Size: 641 783 4105
Fragments arranged by size:
4105 783 641
ScaI AGT'ACT
Cuts at: 0 2844 4440 4849 5529
Size: 2844 1596 409 680
Fragments arranged by size:
2844 1596 680 409
SexAI A'CCwGG_T
Cuts at: 0 146 4397 5181 5529
Size: 146 4251 784 348
Fragments arranged by size:
4251 784 348 146
SfcI C'TryA_G
Cuts at: 0 1454 2167 3710 3759 4006 4517 4550 5456
Size: 1454 713 1543 49 247 511 33 906
Cuts at: 5456 5529
Size: 73
Fragments arranged by size:
1543 1454 906 713 511 247 73 49
33
SmlI C'TyrA_G
Cuts at: 0 841 1707 2209 2794 2893 3214 3571 5016
Size: 841 866 502 585 99 321 357 1445
Cuts at: 5016 5246 5529
Size: 230 283
Fragments arranged by size:
1445 866 841 585 502 357 321 283
230 99
SpeI A'CTAG_T
Cuts at: 0 1315 5529
Size: 1315 4214
StuI AGG'CCT
Cuts at: 0 1978 5529
Size: 1978 3551
StyI C'CwwG_G
Cuts at: 0 4364 4409 5393 5529
Size: 4364 45 984 136
Fragments arranged by size:
4364 984 136 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4278 5529
Size: 4278 1251
TatI w'GTAC_w
Cuts at: 0 2842 4073 4438 4847 5001 5478 5529
Size: 2842 1231 365 409 154 477 51
Fragments arranged by size:
2842 1231 477 409 365 154 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 5529
Size: 220 32 5277
Fragments arranged by size:
5277 220 32
Tth111I GACn'n_nGTC
Cuts at: 0 3270 5183 5529
Size: 3270 1913 346
Fragments arranged by size:
3270 1913 346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4395 5529
Size: 4395 1134
XmnI GAAnn'nnTTC
Cuts at: 0 431 1433 3494 4289 5529
Size: 431 1002 2061 795 1240
Fragments arranged by size:
2061 1240 1002 795 431
ZraI GAC'GTC
Cuts at: 0 4102 5529
Size: 4102 1427
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AleI AloI
AlwNI ApoI AseI BanII BbsI BclI BlpI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BssSI BstAPI BstXI BstYI BtgI BtsI
DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI PasI PflMI PpiI PshAI
PsiI PstI PvuII SacI SalI SapI ScaI SexAI
SfcI SmlI SpeI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflIII AgeI AhdI AlfI ApaI ApaLI
AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI
BmtI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrFI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI EagI EciI EcoO109I FseI FspI FspAI HaeII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpuMI PspOMI PspXI PsrI PvuI RsrII
SacII SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI SwaI XbaI XhoI XmaI