Restriction map for
pFN21AE3486
(Linear) (Six-base) MAPSORT of: KIEE3486.seq Check: 7839 from: 1 to: 5376
KIEE3486.orf
With 182 enzymes: SgfI *
July 3, 2012 17:58 ..
AccI GT'mk_AC
Cuts at: 0 1503 5376
Size: 1503 3873
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 398 3473 3794 3866 3941 4004 4955
Size: 383 15 3075 321 72 75 63 951
Cuts at: 4955 5279 5376
Size: 324 97
Fragments arranged by size:
3075 951 383 324 321 97 75 72
63 15
AflIII A'CryG_T
Cuts at: 0 476 478 883 892 2128 5376
Size: 476 2 405 9 1236 3248
Fragments arranged by size:
3248 1236 476 405 9 2
AleI CACnn'nnGTG
Cuts at: 0 5260 5376
Size: 5260 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3850 5376
Size: 3850 1526
AlwNI CAG_nnn'CTG
Cuts at: 0 836 1222 1822 3004 5257 5376
Size: 836 386 600 1182 2253 119
Fragments arranged by size:
2253 1182 836 600 386 119
ApoI r'AATT_y
Cuts at: 0 19 198 221 578 937 1918 2613 3596
Size: 19 179 23 357 359 981 695 983
Cuts at: 3596 4115 4123 4314 5376
Size: 519 8 191 1062
Fragments arranged by size:
1062 983 981 695 519 359 357 191
179 23 19 8
AvrII C'CTAG_G
Cuts at: 0 556 5376
Size: 556 4820
BamHI G'GATC_C
Cuts at: 0 3778 5376
Size: 3778 1598
BanI G'GyrC_C
Cuts at: 0 1395 5376
Size: 1395 3981
BanII G_rGCy'C
Cuts at: 0 65 3817 5376
Size: 65 3752 1559
Fragments arranged by size:
3752 1559 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 1166 1199 2648 3208 3476 5376
Size: 1166 33 1449 560 268 1900
Fragments arranged by size:
1900 1449 1166 560 268 33
BbvCI CC'TCA_GC
Cuts at: 0 2559 5376
Size: 2559 2817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 386 420 5376
Size: 386 34 4956
Fragments arranged by size:
4956 386 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1257 1703 4094 5376
Size: 1257 446 2391 1282
Fragments arranged by size:
2391 1282 1257 446
BclI T'GATC_A
Cuts at: 0 4629 5376
Size: 4629 747
BglI GCCn_nnn'nGGC
Cuts at: 0 358 5376
Size: 358 5018
BglII A'GATC_T
Cuts at: 0 1075 2116 2245 2311 3151 5376
Size: 1075 1041 129 66 840 2225
Fragments arranged by size:
2225 1075 1041 840 129 66
BlpI GC'TnA_GC
Cuts at: 0 5157 5376
Size: 5157 219
BmgBI CAC'GTC
Cuts at: 0 893 4137 5376
Size: 893 3244 1239
Fragments arranged by size:
3244 1239 893
BmrI ACTGGGnnnn_n'
Cuts at: 0 3653 4820 4842 5376
Size: 3653 1167 22 534
Fragments arranged by size:
3653 1167 534 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3173 5376
Size: 3173 2203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 1643 1787 1799 2561 2567 2756 3842
Size: 1199 444 144 12 762 6 189 1086
Cuts at: 3842 5376
Size: 1534
Fragments arranged by size:
1534 1199 1086 762 444 189 144 12
6
Bpu10I CC'TnA_GC
Cuts at: 0 1997 2559 5376
Size: 1997 562 2817
Fragments arranged by size:
2817 1997 562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1619 2303 2345 4593 5376
Size: 1619 684 42 2248 783
Fragments arranged by size:
2248 1619 783 684 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 1032 2450 5376
Size: 1032 1418 2926
Fragments arranged by size:
2926 1418 1032
BsaAI yAC'GTr
Cuts at: 0 479 5376
Size: 479 4897
BsaBI GATnn'nnATC
Cuts at: 0 2310 4688 5376
Size: 2310 2378 688
Fragments arranged by size:
2378 2310 688
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1703 1733 5376
Size: 1703 30 3643
Fragments arranged by size:
3643 1703 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 736 1001 1341 1590 1691 1727 1742 1790
Size: 736 265 340 249 101 36 15 48
Cuts at: 1790 2306 3146 3205 5376
Size: 516 840 59 2171
Fragments arranged by size:
2171 840 736 516 340 265 249 101
59 48 36 15
BseYI C'CCAG_C
Cuts at: 0 335 1137 1511 1783 5086 5098 5376
Size: 335 802 374 272 3303 12 278
Fragments arranged by size:
3303 802 374 335 278 272 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1879 5376
Size: 1879 3497
BsiHKAI G_wGCw'C
Cuts at: 0 574 881 1337 2547 3817 5376
Size: 574 307 456 1210 1270 1559
Fragments arranged by size:
1559 1270 1210 574 456 307
Bsp1286I G_dGCh'C
Cuts at: 0 65 574 881 1337 2547 3817 5376
Size: 65 509 307 456 1210 1270 1559
Fragments arranged by size:
1559 1270 1210 509 456 307 65
BspHI T'CATG_A
Cuts at: 0 593 4626 5376
Size: 593 4033 750
Fragments arranged by size:
4033 750 593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 143 3117 4041 5376
Size: 143 2974 924 1335
Fragments arranged by size:
2974 1335 924 143
BsrBI CCG'CTC
Cuts at: 0 1369 2162 5376
Size: 1369 793 3214
Fragments arranged by size:
3214 1369 793
BsrDI GCAATG_nn'
Cuts at: 0 404 635 5376
Size: 404 231 4741
Fragments arranged by size:
4741 404 231
BssSI C'ACGA_G
Cuts at: 0 4376 5376
Size: 4376 1000
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1324 5376
Size: 1324 4052
BstEII G'GTnAC_C
Cuts at: 0 1387 2186 2276 5376
Size: 1387 799 90 3100
Fragments arranged by size:
3100 1387 799 90
BstYI r'GATC_y
Cuts at: 0 609 1075 1098 2116 2245 2311 3073 3151
Size: 609 466 23 1018 129 66 762 78
Cuts at: 3151 3778 5376
Size: 627 1598
Fragments arranged by size:
1598 1018 762 627 609 466 129 78
66 23
BtgI C'CryG_G
Cuts at: 0 1599 5376
Size: 1599 3777
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 298 5376
Size: 298 5078
BtsI GCAGTG_nn'
Cuts at: 0 5154 5376
Size: 5154 222
ClaI AT'CG_AT
Cuts at: 0 2262 5376
Size: 2262 3114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1670 1705 3776 3811 5376
Size: 1670 35 2071 35 1565
Fragments arranged by size:
2071 1670 1565 35 35
DraI TTT'AAA
Cuts at: 0 2994 4320 5376
Size: 2994 1326 1056
Fragments arranged by size:
2994 1326 1056
EaeI y'GGCC_r
Cuts at: 0 414 1767 4657 5376
Size: 414 1353 2890 719
Fragments arranged by size:
2890 1353 719 414
EarI CTCTTCn'nnn_
Cuts at: 0 318 1373 1560 1943 2199 4544 4661 4955
Size: 318 1055 187 383 256 2345 117 294
Cuts at: 4955 5376
Size: 421
Fragments arranged by size:
2345 1055 421 383 318 294 256 187
117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1685 5376
Size: 1685 3691
EcoICRI GAG'CTC
Cuts at: 0 3815 5376
Size: 3815 1561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 398 1199 1643 1787 1799 2561 2567
Size: 383 15 801 444 144 12 762 6
Cuts at: 2567 2756 3473 3794 3842 3866 3941 4004 4955
Size: 189 717 321 48 24 75 63 951
Cuts at: 4955 5279 5376
Size: 324 97
Fragments arranged by size:
951 801 762 717 444 383 324 321
189 144 97 75 63 48 24 15
12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 230 1802 3113 4568 5376
Size: 230 1572 1311 1455 808
Fragments arranged by size:
1572 1455 1311 808 230
EcoO109I rG'GnC_Cy
Cuts at: 0 3118 3578 4989 5376
Size: 3118 460 1411 387
Fragments arranged by size:
3118 1411 460 387
EcoRI G'AATT_C
Cuts at: 0 198 221 578 1918 2613 5376
Size: 198 23 357 1340 695 2763
Fragments arranged by size:
2763 1340 695 357 198 23
EcoRV GAT'ATC
Cuts at: 0 1085 2257 5376
Size: 1085 1172 3119
Fragments arranged by size:
3119 1172 1085
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1120 5376
Size: 1120 4256
FspI TGC'GCA
Cuts at: 0 152 5376
Size: 152 5224
HaeII r_GCGC'y
Cuts at: 0 2043 5376
Size: 2043 3333
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 712 744 882 914 1062 1094
Size: 339 32 341 32 138 32 148 32
Cuts at: 1094 1436 1468 2965 2997 3340 3372 3466 3498
Size: 342 32 1497 32 343 32 94 32
Cuts at: 3498 3697 3729 3964 3985 3996 4017 4507 4539
Size: 199 32 235 21 11 21 490 32
Cuts at: 4539 4660 4692 5376
Size: 121 32 684
Fragments arranged by size:
1497 684 490 343 342 341 339 235
199 148 138 121 94 32 32 32
32 32 32 32 32 32 32 32
21 21 11
HincII GTy'rAC
Cuts at: 0 1504 2448 3710 5376
Size: 1504 944 1262 1666
Fragments arranged by size:
1666 1504 1262 944
HindIII A'AGCT_T
Cuts at: 0 2701 5376
Size: 2701 2675
MfeI C'AATT_G
Cuts at: 0 302 686 1355 5376
Size: 302 384 669 4021
Fragments arranged by size:
4021 669 384 302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1547 4211 5376
Size: 913 634 2664 1165
Fragments arranged by size:
2664 1165 913 634
MscI TGG'CCA
Cuts at: 0 416 1769 5376
Size: 416 1353 3607
Fragments arranged by size:
3607 1353 416
MslI CAynn'nnrTG
Cuts at: 0 902 5260 5376
Size: 902 4358 116
Fragments arranged by size:
4358 902 116
MspA1I CmG'CkG
Cuts at: 0 1315 1783 3919 5376
Size: 1315 468 2136 1457
Fragments arranged by size:
2136 1457 1315 468
NdeI CA'TA_TG
Cuts at: 0 258 681 2415 5376
Size: 258 423 1734 2961
Fragments arranged by size:
2961 1734 423 258
NspI r_CATG'y
Cuts at: 0 887 4717 5251 5376
Size: 887 3830 534 125
Fragments arranged by size:
3830 887 534 125
PciI A'CATG_T
Cuts at: 0 883 5376
Size: 883 4493
PflMI CCAn_nnn'nTGG
Cuts at: 0 529 1498 1783 2779 5376
Size: 529 969 285 996 2597
Fragments arranged by size:
2597 996 969 529 285
PfoI T'CCnGG_A
Cuts at: 0 863 2772 5376
Size: 863 1909 2604
Fragments arranged by size:
2604 1909 863
PmlI CAC'GTG
Cuts at: 0 479 5376
Size: 479 4897
PpuMI rG'GwC_Cy
Cuts at: 0 3118 3578 4989 5376
Size: 3118 460 1411 387
Fragments arranged by size:
3118 1411 460 387
PsiI TTA'TAA
Cuts at: 0 17 265 2525 4045 5376
Size: 17 248 2260 1520 1331
Fragments arranged by size:
2260 1520 1331 248 17
PstI C_TGCA'G
Cuts at: 0 140 1320 5376
Size: 140 1180 4056
Fragments arranged by size:
4056 1180 140
PvuII CAG'CTG
Cuts at: 0 1315 3919 5376
Size: 1315 2604 1457
Fragments arranged by size:
2604 1457 1315
SacI G_AGCT'C
Cuts at: 0 3817 5376
Size: 3817 1559
SalI G'TCGA_C
Cuts at: 0 1502 5376
Size: 1502 3874
ScaI AGT'ACT
Cuts at: 0 4830 5376
Size: 4830 546
SexAI A'CCwGG_T
Cuts at: 0 1391 5376
Size: 1391 3985
SfcI C'TryA_G
Cuts at: 0 136 665 1316 1584 1923 2067 2888 4679
Size: 136 529 651 268 339 144 821 1791
Cuts at: 4679 5376
Size: 697
Fragments arranged by size:
1791 821 697 651 529 339 268 144
136
SmlI C'TyrA_G
Cuts at: 0 1634 2318 2360 4608 5376
Size: 1634 684 42 2248 768
Fragments arranged by size:
2248 1634 768 684 42
SspI AAT'ATT
Cuts at: 0 1307 2383 2521 3099 5376
Size: 1307 1076 138 578 2277
Fragments arranged by size:
2277 1307 1076 578 138
StyI C'CwwG_G
Cuts at: 0 556 1178 1840 4130 4543 5376
Size: 556 622 662 2290 413 833
Fragments arranged by size:
2290 833 662 622 556 413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1151 5376
Size: 1151 4225
TatI w'GTAC_w
Cuts at: 0 3661 4057 4828 5376
Size: 3661 396 771 548
Fragments arranged by size:
3661 771 548 396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 564 596 1449 1481 1703 1735 3065 3097
Size: 564 32 853 32 222 32 1330 32
Cuts at: 3097 5376
Size: 2279
Fragments arranged by size:
2279 1330 853 564 222 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 2242 4864 5376
Size: 2242 2622 512
Fragments arranged by size:
2622 2242 512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3789 5116 5376
Size: 3789 1327 260
Fragments arranged by size:
3789 1327 260
XmnI GAAnn'nnTTC
Cuts at: 0 4980 5376
Size: 4980 396
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AvrII
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NdeI NspI PciI PflMI
PfoI PmlI PpuMI PsiI PstI PvuII SacI SalI
ScaI SexAI SfcI SmlI SspI StyI TaqII TatI
TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI BaeI
BbeI BfrBI Bme1580I BmtI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrFI BsrGI BssHII BstBI BstXI
BstZ17I Bsu36I DraIII DrdI EagI FseI FspAI HpaI
KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XhoI XmaI ZraI