Restriction map for
pFN21AE3477
(Linear) (Six-base) MAPSORT of: KIEE3477.seq Check: 791 from: 1 to: 4812
KIEE3477.orf
With 182 enzymes: SgfI *
July 3, 2012 17:57 ..
Acc65I G'GTAC_C
Cuts at: 0 3936 4812
Size: 3936 876
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 1427 2575 4812
Size: 103 1324 1148 2237
Fragments arranged by size:
2237 1324 1148 103
AfeI AGC'GCT
Cuts at: 0 4098 4812
Size: 4098 714
AflII C'TTAA_G
Cuts at: 0 3844 4812
Size: 3844 968
AflIII A'CryG_T
Cuts at: 0 2683 4812
Size: 2683 2129
AhdI GACnn_n'nnGTC
Cuts at: 0 4779 4812
Size: 4779 33
AleI CACnn'nnGTG
Cuts at: 0 3136 4812
Size: 3136 1676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1482 4563 4812
Size: 1482 3081 249
Fragments arranged by size:
3081 1482 249
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3251 3283 4143 4175 4812
Size: 3251 32 860 32 637
Fragments arranged by size:
3251 860 637 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1420 1693 2271 3533 4634 4812
Size: 1420 273 578 1262 1101 178
Fragments arranged by size:
1420 1262 1101 578 273 178
ApoI r'AATT_y
Cuts at: 0 808 1378 1949 2987 3898 4559 4812
Size: 808 570 571 1038 911 661 253
Fragments arranged by size:
1038 911 808 661 571 570 253
AvaI C'yCGr_G
Cuts at: 0 3905 4812
Size: 3905 907
BamHI G'GATC_C
Cuts at: 0 3597 4812
Size: 3597 1215
BanI G'GyrC_C
Cuts at: 0 4 3506 3936 4812
Size: 4 3502 430 876
Fragments arranged by size:
3502 876 430 4
BanII G_rGCy'C
Cuts at: 0 3394 3695 3995 4004 4812
Size: 3394 301 300 9 808
Fragments arranged by size:
3394 808 301 300 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1016 2500 3560 4487 4778 4812
Size: 1016 1484 1060 927 291 34
Fragments arranged by size:
1484 1060 1016 927 291 34
BbvCI CC'TCA_GC
Cuts at: 0 31 49 182 4812
Size: 31 18 133 4630
Fragments arranged by size:
4630 133 31 18
BciVI GTATCCnnnnn_n'
Cuts at: 0 3387 4812
Size: 3387 1425
BglII A'GATC_T
Cuts at: 0 1019 2517 2662 4812
Size: 1019 1498 145 2150
Fragments arranged by size:
2150 1498 1019 145
BlpI GC'TnA_GC
Cuts at: 0 3789 4812
Size: 3789 1023
Bme1580I G_kGCm'C
Cuts at: 0 3511 4812
Size: 3511 1301
BmgBI CAC'GTC
Cuts at: 0 4065 4812
Size: 4065 747
BmrI ACTGGGnnnn_n'
Cuts at: 0 2589 4812
Size: 2589 2223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2408 4812
Size: 2408 2404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 578 837 1044 3834 4149 4812
Size: 515 63 259 207 2790 315 663
Fragments arranged by size:
2790 663 515 315 259 207 63
Bpu10I CC'TnA_GC
Cuts at: 0 31 49 182 4072 4812
Size: 31 18 133 3890 740
Fragments arranged by size:
3890 740 133 31 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1577 2223 2651 4353 4812
Size: 616 961 646 428 1702 459
Fragments arranged by size:
1702 961 646 616 459 428
BsaI GGTCTCn'nnnn_
Cuts at: 0 3938 4812
Size: 3938 874
BsaAI yAC'GTr
Cuts at: 0 363 1359 4812
Size: 363 996 3453
Fragments arranged by size:
3453 996 363
BsaBI GATnn'nnATC
Cuts at: 0 725 2048 2522 3596 4812
Size: 725 1323 474 1074 1216
Fragments arranged by size:
1323 1216 1074 725 474
BsaHI Gr'CG_yC
Cuts at: 0 4328 4812
Size: 4328 484
BsaWI w'CCGG_w
Cuts at: 0 1110 4812
Size: 1110 3702
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 239 269 4812
Size: 239 30 4543
Fragments arranged by size:
4543 239 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 66 2146 3033 4224 4370 4812
Size: 55 11 2080 887 1191 146 442
Fragments arranged by size:
2080 1191 887 442 146 55 11
BseYI C'CCAG_C
Cuts at: 0 1515 3292 4051 4303 4350 4812
Size: 1515 1777 759 252 47 462
Fragments arranged by size:
1777 1515 759 462 252 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 2392 3499 4812
Size: 208 2184 1107 1313
Fragments arranged by size:
2184 1313 1107 208
BsiHKAI G_wGCw'C
Cuts at: 0 1707 3995 4070 4812
Size: 1707 2288 75 742
Fragments arranged by size:
2288 1707 742 75
BsmI GAATG_Cn'
Cuts at: 0 359 975 1890 1937 3128 3311 4812
Size: 359 616 915 47 1191 183 1501
Fragments arranged by size:
1501 1191 915 616 359 183 47
Bsp1286I G_dGCh'C
Cuts at: 0 1707 3394 3511 3695 3995 4004 4070 4812
Size: 1707 1687 117 184 300 9 66 742
Fragments arranged by size:
1707 1687 742 300 184 117 66 9
BspEI T'CCGG_A
Cuts at: 0 1110 4812
Size: 1110 3702
BspHI T'CATG_A
Cuts at: 0 2231 2511 4812
Size: 2231 280 2301
Fragments arranged by size:
2301 2231 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1148 1314 4812
Size: 1148 166 3498
Fragments arranged by size:
3498 1148 166
BsrDI GCAATG_nn'
Cuts at: 0 3227 4446 4458 4812
Size: 3227 1219 12 354
Fragments arranged by size:
3227 1219 354 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2440 4812
Size: 2440 2372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 990 1251 3702 4011 4812
Size: 990 261 2451 309 801
Fragments arranged by size:
2451 990 801 309 261
BstYI r'GATC_y
Cuts at: 0 939 1019 2517 2662 3597 3807 4812
Size: 939 80 1498 145 935 210 1005
Fragments arranged by size:
1498 1005 939 935 210 145 80
Bsu36I CC'TnA_GG
Cuts at: 0 2129 2933 3404 4382 4812
Size: 2129 804 471 978 430
Fragments arranged by size:
2129 978 804 471 430
BtgI C'CryG_G
Cuts at: 0 2649 3511 4195 4812
Size: 2649 862 684 617
Fragments arranged by size:
2649 862 684 617
BtsI GCAGTG_nn'
Cuts at: 0 132 1210 3783 4812
Size: 132 1078 2573 1029
Fragments arranged by size:
2573 1078 1029 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2618 2653 2667 2702 4184 4219 4812
Size: 2618 35 14 35 1482 35 593
Fragments arranged by size:
2618 1482 593 35 35 35 14
DraI TTT'AAA
Cuts at: 0 1895 2846 4812
Size: 1895 951 1966
Fragments arranged by size:
1966 1895 951
DraIII CAC_nnn'GTG
Cuts at: 0 366 4812
Size: 366 4446
DrdI GACnn_nn'nnGTC
Cuts at: 0 1842 4812
Size: 1842 2970
EaeI y'GGCC_r
Cuts at: 0 4403 4812
Size: 4403 409
EarI CTCTTCn'nnn_
Cuts at: 0 272 1367 1662 1813 2039 3051 3203 3731
Size: 272 1095 295 151 226 1012 152 528
Cuts at: 3731 4052 4812
Size: 321 760
Fragments arranged by size:
1095 1012 760 528 321 295 272 226
152 151
EcoICRI GAG'CTC
Cuts at: 0 3993 4812
Size: 3993 819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 515 578 837 1044 1427 2575 3834
Size: 103 412 63 259 207 383 1148 1259
Cuts at: 3834 4149 4812
Size: 315 663
Fragments arranged by size:
1259 1148 663 412 383 315 259 207
103 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 780 1069 4812
Size: 780 289 3743
Fragments arranged by size:
3743 780 289
EcoO109I rG'GnC_Cy
Cuts at: 0 101 787 3090 4812
Size: 101 686 2303 1722
Fragments arranged by size:
2303 1722 686 101
EcoRI G'AATT_C
Cuts at: 0 4559 4812
Size: 4559 253
EcoRV GAT'ATC
Cuts at: 0 758 4812
Size: 758 4054
FspI TGC'GCA
Cuts at: 0 1978 4040 4812
Size: 1978 2062 772
Fragments arranged by size:
2062 1978 772
HaeII r_GCGC'y
Cuts at: 0 49 4100 4812
Size: 49 4051 712
Fragments arranged by size:
4051 712 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 239 271 981 1013 1252 1284 1736 1768
Size: 239 32 710 32 239 32 452 32
Cuts at: 1768 2122 2154 3028 3060 3478 3510 3979 4011
Size: 354 32 874 32 418 32 469 32
Cuts at: 4011 4654 4686 4765 4797 4812
Size: 643 32 79 32 15
Fragments arranged by size:
874 710 643 469 452 418 354 239
239 79 32 32 32 32 32 32
32 32 32 32 15
HincII GTy'rAC
Cuts at: 0 834 4812
Size: 834 3978
HindIII A'AGCT_T
Cuts at: 0 2416 4812
Size: 2416 2396
KpnI G_GTAC'C
Cuts at: 0 3940 4812
Size: 3940 872
MfeI C'AATT_G
Cuts at: 0 2630 4812
Size: 2630 2182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 2939 3049 4812
Size: 225 2714 110 1763
Fragments arranged by size:
2714 1763 225 110
MscI TGG'CCA
Cuts at: 0 4405 4812
Size: 4405 407
MslI CAynn'nnrTG
Cuts at: 0 988 1198 1718 1855 2303 2332 2696 3136
Size: 988 210 520 137 448 29 364 440
Cuts at: 3136 3659 4652 4812
Size: 523 993 160
Fragments arranged by size:
993 988 523 520 448 440 364 210
160 137 29
MspA1I CmG'CkG
Cuts at: 0 1430 1656 2856 3710 4157 4634 4812
Size: 1430 226 1200 854 447 477 178
Fragments arranged by size:
1430 1200 854 477 447 226 178
NcoI C'CATG_G
Cuts at: 0 2649 3511 4812
Size: 2649 862 1301
Fragments arranged by size:
2649 1301 862
NdeI CA'TA_TG
Cuts at: 0 935 2345 2544 4812
Size: 935 1410 199 2268
Fragments arranged by size:
2268 1410 935 199
NspI r_CATG'y
Cuts at: 0 2228 2687 3192 4348 4436 4812
Size: 2228 459 505 1156 88 376
Fragments arranged by size:
2228 1156 505 459 376 88
PasI CC'CwG_GG
Cuts at: 0 3981 4812
Size: 3981 831
PciI A'CATG_T
Cuts at: 0 2683 4812
Size: 2683 2129
PflMI CCAn_nnn'nTGG
Cuts at: 0 366 552 1205 1317 4812
Size: 366 186 653 112 3495
Fragments arranged by size:
3495 653 366 186 112
PfoI T'CCnGG_A
Cuts at: 0 143 628 2075 4812
Size: 143 485 1447 2737
Fragments arranged by size:
2737 1447 485 143
PmlI CAC'GTG
Cuts at: 0 363 4812
Size: 363 4449
PpuMI rG'GwC_Cy
Cuts at: 0 101 787 3090 4812
Size: 101 686 2303 1722
Fragments arranged by size:
2303 1722 686 101
PshAI GACnn'nnGTC
Cuts at: 0 3710 4812
Size: 3710 1102
PsiI TTA'TAA
Cuts at: 0 4529 4812
Size: 4529 283
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2087 2119 4812
Size: 2087 32 2693
Fragments arranged by size:
2693 2087 32
PstI C_TGCA'G
Cuts at: 0 1308 2595 4812
Size: 1308 1287 2217
Fragments arranged by size:
2217 1308 1287
PvuII CAG'CTG
Cuts at: 0 1656 2856 3710 4157 4634 4812
Size: 1656 1200 854 447 477 178
Fragments arranged by size:
1656 1200 854 477 447 178
SacI G_AGCT'C
Cuts at: 0 3995 4812
Size: 3995 817
SanDI GG'GwC_CC
Cuts at: 0 787 4812
Size: 787 4025
SapI GCTCTTCn'nnn_
Cuts at: 0 3731 4812
Size: 3731 1081
ScaI AGT'ACT
Cuts at: 0 4798 4812
Size: 4798 14
SexAI A'CCwGG_T
Cuts at: 0 2090 4812
Size: 2090 2722
SfcI C'TryA_G
Cuts at: 0 1304 1367 2591 4446 4812
Size: 1304 63 1224 1855 366
Fragments arranged by size:
1855 1304 1224 366 63
SmlI C'TyrA_G
Cuts at: 0 595 1592 2238 2666 3844 4332 4812
Size: 595 997 646 428 1178 488 480
Fragments arranged by size:
1178 997 646 595 488 480 428
SspI AAT'ATT
Cuts at: 0 1533 2065 3107 4812
Size: 1533 532 1042 1705
Fragments arranged by size:
1705 1533 1042 532
StuI AGG'CCT
Cuts at: 0 2599 4812
Size: 2599 2213
StyI C'CwwG_G
Cuts at: 0 8 2649 3511 4812
Size: 8 2641 862 1301
Fragments arranged by size:
2641 1301 862 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1845 3840 4555 4812
Size: 1845 1995 715 257
Fragments arranged by size:
1995 1845 715 257
TatI w'GTAC_w
Cuts at: 0 2834 2904 3325 3758 4796 4812
Size: 2834 70 421 433 1038 16
Fragments arranged by size:
2834 1038 433 421 70 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3483 3515 4812
Size: 3483 32 1297
Fragments arranged by size:
3483 1297 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 367 553 1694 4607 4812
Size: 367 186 1141 2913 205
Fragments arranged by size:
2913 1141 367 205 186
XmnI GAAnn'nnTTC
Cuts at: 0 400 1790 3160 4812
Size: 400 1390 1370 1652
Fragments arranged by size:
1652 1390 1370 400
Enzymes that do cut:
Acc65I AcuI AfeI AflII AflIII AhdI AleI AlfI
AloI AlwNI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BciVI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BstAPI BstXI BstYI Bsu36I BtgI
BtsI CspCI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NcoI NdeI NspI PasI PciI PflMI PfoI PmlI
PpuMI PshAI PsiI PsrI PstI PvuII SacI SanDI
SapI ScaI SexAI SfcI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AgeI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI
BglI BmtI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI EagI
EciI FalI FseI FspAI HpaI KasI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PmeI
PpiI PspOMI PspXI PvuI RsrII SacII SalI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XhoI XmaI ZraI