Restriction map for
pFN21AE3469
(Linear) (Six-base) MAPSORT of: KIEE3469.seq Check: 1495 from: 1 to: 4353
KIEE3469.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1055 4353
Size: 1055 3298
AccI GT'mk_AC
Cuts at: 0 518 4353
Size: 518 3835
Acc65I G'GTAC_C
Cuts at: 0 46 4353
Size: 46 4307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 723 1531 1607 3344 3506 4058 4280
Size: 508 215 808 76 1737 162 552 222
Cuts at: 4280 4353
Size: 73
Fragments arranged by size:
1737 808 552 508 222 215 162 76
73
AfeI AGC'GCT
Cuts at: 0 436 4353
Size: 436 3917
AflII C'TTAA_G
Cuts at: 0 51 127 4353
Size: 51 76 4226
Fragments arranged by size:
4226 76 51
AflIII A'CryG_T
Cuts at: 0 1172 2153 4353
Size: 1172 981 2200
Fragments arranged by size:
2200 1172 981
AleI CACnn'nnGTG
Cuts at: 0 3145 4353
Size: 3145 1208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 455 853 901 4353
Size: 455 398 48 3452
Fragments arranged by size:
3452 455 398 48
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2140 2172 4353
Size: 2140 32 2181
Fragments arranged by size:
2181 2140 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1924 2821 4353
Size: 1924 897 1532
Fragments arranged by size:
1924 1532 897
ApaI G_GGCC'C
Cuts at: 0 2368 4353
Size: 2368 1985
ApaLI G'TGCA_C
Cuts at: 0 1042 2520 2630 4353
Size: 1042 1478 110 1723
Fragments arranged by size:
1723 1478 1042 110
ApoI r'AATT_y
Cuts at: 0 122 137 208 3263 3607 4353
Size: 122 15 71 3055 344 746
Fragments arranged by size:
3055 746 344 122 71 15
AvaI C'yCGr_G
Cuts at: 0 5 1214 1389 3174 4353
Size: 5 1209 175 1785 1179
Fragments arranged by size:
1785 1209 1179 175 5
BamHI G'GATC_C
Cuts at: 0 308 2558 3745 4353
Size: 308 2250 1187 608
Fragments arranged by size:
2250 1187 608 308
BanI G'GyrC_C
Cuts at: 0 46 890 3077 4353
Size: 46 844 2187 1276
Fragments arranged by size:
2187 1276 844 46
BanII G_rGCy'C
Cuts at: 0 725 1542 1567 2041 2368 2894 2926 2981
Size: 725 817 25 474 327 526 32 55
Cuts at: 2981 3890 3910 4068 4150 4353
Size: 909 20 158 82 203
Fragments arranged by size:
909 817 725 526 474 327 203 158
82 55 32 25 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 382 2589 3719 4353
Size: 10 372 2207 1130 634
Fragments arranged by size:
2207 1130 634 372 10
BbvCI CC'TCA_GC
Cuts at: 0 1111 2242 4353
Size: 1111 1131 2111
Fragments arranged by size:
2111 1131 1111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 275 309 954 988 4353
Size: 275 34 645 34 3365
Fragments arranged by size:
3365 645 275 34 34
BclI T'GATC_A
Cuts at: 0 1313 4353
Size: 1313 3040
BglII A'GATC_T
Cuts at: 0 1871 4041 4353
Size: 1871 2170 312
Fragments arranged by size:
2170 1871 312
BlpI GC'TnA_GC
Cuts at: 0 74 4353
Size: 74 4279
Bme1580I G_kGCm'C
Cuts at: 0 1046 1223 2368 2524 2634 4353
Size: 1046 177 1145 156 110 1719
Fragments arranged by size:
1719 1145 1046 177 156 110
BmgBI CAC'GTC
Cuts at: 0 1461 4353
Size: 1461 2892
BmrI ACTGGGnnnn_n'
Cuts at: 0 656 1005 1012 1254 2085 2697 4353
Size: 656 349 7 242 831 612 1656
Fragments arranged by size:
1656 831 656 612 349 242 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1893 3603 4353
Size: 1893 1710 750
Fragments arranged by size:
1893 1710 750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1039 1186 1556 1901 4353
Size: 611 428 147 370 345 2452
Fragments arranged by size:
2452 611 428 370 345 147
Bpu10I CC'TnA_GC
Cuts at: 0 313 1111 2242 4353
Size: 313 798 1131 2111
Fragments arranged by size:
2111 1131 798 313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2510 3212 4353
Size: 2510 702 1141
Fragments arranged by size:
2510 1141 702
BsaI GGTCTCn'nnnn_
Cuts at: 0 3302 4353
Size: 3302 1051
BsaBI GATnn'nnATC
Cuts at: 0 245 4353
Size: 245 4108
BsaHI Gr'CG_yC
Cuts at: 0 2897 4353
Size: 2897 1456
BsaWI w'CCGG_w
Cuts at: 0 681 1499 4353
Size: 681 818 2854
Fragments arranged by size:
2854 818 681
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1159 1189 2823 2853 4353
Size: 1159 30 1634 30 1500
Fragments arranged by size:
1634 1500 1159 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2182 3962 4129 4353
Size: 2182 1780 167 224
Fragments arranged by size:
2182 1780 224 167
BseYI C'CCAG_C
Cuts at: 0 1828 2677 3751 4353
Size: 1828 849 1074 602
Fragments arranged by size:
1828 1074 849 602
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1009 1594 4353
Size: 568 441 585 2759
Fragments arranged by size:
2759 585 568 441
BsiHKAI G_wGCw'C
Cuts at: 0 271 1046 2041 2524 2634 3890 4068 4353
Size: 271 775 995 483 110 1256 178 285
Fragments arranged by size:
1256 995 775 483 285 271 178 110
BsmI GAATG_Cn'
Cuts at: 0 2471 3282 4353
Size: 2471 811 1071
Fragments arranged by size:
2471 1071 811
Bsp1286I G_dGCh'C
Cuts at: 0 271 725 1046 1223 1542 1567 2041 2368
Size: 271 454 321 177 319 25 474 327
Cuts at: 2368 2524 2634 2894 2926 2981 3890 3910 4068
Size: 156 110 260 32 55 909 20 158
Cuts at: 4068 4150 4353
Size: 82 203
Fragments arranged by size:
909 474 454 327 321 319 271 260
203 177 158 156 110 82 55 32
25 20
BspHI T'CATG_A
Cuts at: 0 81 758 996 4353
Size: 81 677 238 3357
Fragments arranged by size:
3357 677 238 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 1055 1088 4353
Size: 350 705 33 3265
Fragments arranged by size:
3265 705 350 33
BsrBI CCG'CTC
Cuts at: 0 1106 4353
Size: 1106 3247
BsrFI r'CCGG_y
Cuts at: 0 2654 4353
Size: 2654 1699
BsrGI T'GTAC_A
Cuts at: 0 1197 4353
Size: 1197 3156
BssSI C'ACGA_G
Cuts at: 0 544 2224 4353
Size: 544 1680 2129
Fragments arranged by size:
2129 1680 544
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1050 4353
Size: 1050 3303
BstBI TT'CG_AA
Cuts at: 0 1859 4353
Size: 1859 2494
BstEII G'GTnAC_C
Cuts at: 0 337 4353
Size: 337 4016
BstYI r'GATC_y
Cuts at: 0 240 308 673 1731 1746 1871 2196 2558
Size: 240 68 365 1058 15 125 325 362
Cuts at: 2558 3745 4041 4353
Size: 1187 296 312
Fragments arranged by size:
1187 1058 365 362 325 312 296 240
125 68 15
Bsu36I CC'TnA_GG
Cuts at: 0 202 1736 4112 4353
Size: 202 1534 2376 241
Fragments arranged by size:
2376 1534 241 202
BtgI C'CryG_G
Cuts at: 0 1976 2360 3914 4353
Size: 1976 384 1554 439
Fragments arranged by size:
1976 1554 439 384
BtsI GCAGTG_nn'
Cuts at: 0 825 2031 2597 3953 4353
Size: 825 1206 566 1356 400
Fragments arranged by size:
1356 1206 825 566 400
DraI TTT'AAA
Cuts at: 0 2378 4353
Size: 2378 1975
DrdI GACnn_nn'nnGTC
Cuts at: 0 3710 4353
Size: 3710 643
EaeI y'GGCC_r
Cuts at: 0 845 1760 2357 4353
Size: 845 915 597 1996
Fragments arranged by size:
1996 915 845 597
EarI CTCTTCn'nnn_
Cuts at: 0 683 1808 1904 1979 2046 2291 2641 3182
Size: 683 1125 96 75 67 245 350 541
Cuts at: 3182 3332 4184 4353
Size: 150 852 169
Fragments arranged by size:
1125 852 683 541 350 245 169 150
96 75 67
EcoICRI GAG'CTC
Cuts at: 0 2039 3888 4066 4353
Size: 2039 1849 178 287
Fragments arranged by size:
2039 1849 287 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 611 723 1039 1186 1531 1556 1607
Size: 508 103 112 316 147 345 25 51
Cuts at: 1607 1901 3344 3506 4058 4280 4353
Size: 294 1443 162 552 222 73
Fragments arranged by size:
1443 552 508 345 316 294 222 162
147 112 103 73 51 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 3760 4141 4353
Size: 547 3213 381 212
Fragments arranged by size:
3213 547 381 212
EcoO109I rG'GnC_Cy
Cuts at: 0 540 933 1206 1740 2365 2662 4086 4353
Size: 540 393 273 534 625 297 1424 267
Fragments arranged by size:
1424 625 540 534 393 297 273 267
EcoRV GAT'ATC
Cuts at: 0 1061 1497 4353
Size: 1061 436 2856
Fragments arranged by size:
2856 1061 436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 94 1536 2790 3565 3743 4353
Size: 94 1442 1254 775 178 610
Fragments arranged by size:
1442 1254 775 610 178 94
HaeII r_GCGC'y
Cuts at: 0 438 4353
Size: 438 3915
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 651 683 1571 1603 1716 1748
Size: 220 32 399 32 888 32 113 32
Cuts at: 1748 1761 1793 2023 2055 2290 2322 4353
Size: 13 32 230 32 235 32 2031
Fragments arranged by size:
2031 888 399 235 230 220 113 32
32 32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 0 156 1684 2123 4353
Size: 156 1528 439 2230
Fragments arranged by size:
2230 1528 439 156
HindIII A'AGCT_T
Cuts at: 0 3060 3446 4353
Size: 3060 386 907
Fragments arranged by size:
3060 907 386
HpaI GTT'AAC
Cuts at: 0 156 4353
Size: 156 4197
KpnI G_GTAC'C
Cuts at: 0 50 4353
Size: 50 4303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1157 4353
Size: 1157 3196
MscI TGG'CCA
Cuts at: 0 1762 4353
Size: 1762 2591
MslI CAynn'nnrTG
Cuts at: 0 86 757 1033 1595 2228 3145 3842 4353
Size: 86 671 276 562 633 917 697 511
Fragments arranged by size:
917 697 671 633 562 511 276 86
MspA1I CmG'CkG
Cuts at: 0 254 510 743 2681 3526 4353
Size: 254 256 233 1938 845 827
Fragments arranged by size:
1938 845 827 256 254 233
NcoI C'CATG_G
Cuts at: 0 1976 4353
Size: 1976 2377
NspI r_CATG'y
Cuts at: 0 453 1176 2157 2307 3770 4353
Size: 453 723 981 150 1463 583
Fragments arranged by size:
1463 981 723 583 453 150
PasI CC'CwG_GG
Cuts at: 0 3508 4353
Size: 3508 845
PciI A'CATG_T
Cuts at: 0 1172 2153 4353
Size: 1172 981 2200
Fragments arranged by size:
2200 1172 981
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 4353
Size: 420 3933
PfoI T'CCnGG_A
Cuts at: 0 1917 4315 4353
Size: 1917 2398 38
Fragments arranged by size:
2398 1917 38
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3232 3264 4353
Size: 3232 32 1089
Fragments arranged by size:
3232 1089 32
PpuMI rG'GwC_Cy
Cuts at: 0 933 1206 2662 4086 4353
Size: 933 273 1456 1424 267
Fragments arranged by size:
1456 1424 933 273 267
PsiI TTA'TAA
Cuts at: 0 3637 4353
Size: 3637 716
PspOMI G'GGCC_C
Cuts at: 0 2364 4353
Size: 2364 1989
PspXI vC'TCGA_Gb
Cuts at: 0 1214 3174 4353
Size: 1214 1960 1179
Fragments arranged by size:
1960 1214 1179
PstI C_TGCA'G
Cuts at: 0 832 1855 2909 4106 4353
Size: 832 1023 1054 1197 247
Fragments arranged by size:
1197 1054 1023 832 247
PvuII CAG'CTG
Cuts at: 0 2681 3526 4353
Size: 2681 845 827
Fragments arranged by size:
2681 845 827
SacI G_AGCT'C
Cuts at: 0 2041 3890 4068 4353
Size: 2041 1849 178 285
Fragments arranged by size:
2041 1849 285 178
SapI GCTCTTCn'nnn_
Cuts at: 0 1904 1979 2046 2291 4353
Size: 1904 75 67 245 2062
Fragments arranged by size:
2062 1904 245 75 67
ScaI AGT'ACT
Cuts at: 0 2062 3321 4353
Size: 2062 1259 1032
Fragments arranged by size:
2062 1259 1032
SexAI A'CCwGG_T
Cuts at: 0 1955 4353
Size: 1955 2398
SfcI C'TryA_G
Cuts at: 0 519 615 828 904 1299 1851 2398 2905
Size: 519 96 213 76 395 552 547 507
Cuts at: 2905 3152 4102 4353
Size: 247 950 251
Fragments arranged by size:
950 552 547 519 507 395 251 247
213 96 76
SmlI C'TyrA_G
Cuts at: 0 51 127 1214 2525 3174 3227 4353
Size: 51 76 1087 1311 649 53 1126
Fragments arranged by size:
1311 1126 1087 649 76 53 51
StyI C'CwwG_G
Cuts at: 0 161 1696 1976 2534 2842 4142 4353
Size: 161 1535 280 558 308 1300 211
Fragments arranged by size:
1535 1300 558 308 280 211 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 427 832 836 1490 2691 3095 3477 3808
Size: 427 405 4 654 1201 404 382 331
Cuts at: 3808 4353
Size: 545
Fragments arranged by size:
1201 654 545 427 405 404 382 331
4
TatI w'GTAC_w
Cuts at: 0 1169 1197 2060 3319 4353
Size: 1169 28 863 1259 1034
Fragments arranged by size:
1259 1169 1034 863 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2823 2855 4353
Size: 2823 32 1498
Fragments arranged by size:
2823 1498 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2541 2706 4353
Size: 2541 165 1647
Fragments arranged by size:
2541 1647 165
XhoI C'TCGA_G
Cuts at: 0 1214 3174 4353
Size: 1214 1960 1179
Fragments arranged by size:
1960 1214 1179
XmnI GAAnn'nnTTC
Cuts at: 0 704 2513 3611 4353
Size: 704 1809 1098 742
Fragments arranged by size:
1809 1098 742 704
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtsI DraI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII HindIII HpaI KpnI MmeI
MscI MslI MspA1I NcoI NspI PasI PciI PflMI
PfoI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII
SacI SapI ScaI SexAI SfcI SmlI StyI TaqII
TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AgeI AhdI AscI AseI AsiSI AvrII
BaeI BbeI BciVI BfrBI BglI BmtI BsaAI BsiEI
BsiWI BsmBI BspEI BsrDI BssHII BstXI BstZ17I BtgZI
ClaI CspCI DraIII EagI EciI EcoRI FseI FspI
FspAI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PshAI
PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XmaI ZraI