Restriction map for
pFN21AE3464
(Linear) (Six-base) MAPSORT of: KIEE3464.seq Check: 6520 from: 1 to: 4110
KIEE3464.orf
With 182 enzymes: SgfI *
March 22, 2012 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 776 3217 4110
Size: 776 2441 893
Fragments arranged by size:
2441 893 776
AatII G_ACGT'C
Cuts at: 0 1057 2813 4110
Size: 1057 1756 1297
Fragments arranged by size:
1756 1297 1057
AccI GT'mk_AC
Cuts at: 0 1676 4110
Size: 1676 2434
Acc65I G'GTAC_C
Cuts at: 0 562 4110
Size: 562 3548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 831 1477 1939 2053 2093 2179 2522
Size: 316 515 646 462 114 40 86 343
Cuts at: 2522 2850 3316 3607 4110
Size: 328 466 291 503
Fragments arranged by size:
646 515 503 466 462 343 328 316
291 114 86 40
AflIII A'CryG_T
Cuts at: 0 3617 4110
Size: 3617 493
AhdI GACnn_n'nnGTC
Cuts at: 0 635 2814 3621 4110
Size: 635 2179 807 489
Fragments arranged by size:
2179 807 635 489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2443 3568 4110
Size: 2443 1125 542
Fragments arranged by size:
2443 1125 542
AlwNI CAG_nnn'CTG
Cuts at: 0 854 864 2157 3328 4110
Size: 854 10 1293 1171 782
Fragments arranged by size:
1293 1171 854 782 10
ApaLI G'TGCA_C
Cuts at: 0 981 4110
Size: 981 3129
ApoI r'AATT_y
Cuts at: 0 963 1169 1501 3703 4110
Size: 963 206 332 2202 407
Fragments arranged by size:
2202 963 407 332 206
AvaI C'yCGr_G
Cuts at: 0 286 419 2043 3020 3898 4110
Size: 286 133 1624 977 878 212
Fragments arranged by size:
1624 977 878 286 212 133
BamHI G'GATC_C
Cuts at: 0 1878 4110
Size: 1878 2232
BanI G'GyrC_C
Cuts at: 0 70 562 1430 3573 4110
Size: 70 492 868 2143 537
Fragments arranged by size:
2143 868 537 492 70
BanII G_rGCy'C
Cuts at: 0 287 455 1070 2594 3061 4110
Size: 287 168 615 1524 467 1049
Fragments arranged by size:
1524 1049 615 467 287 168
BbeI G_GCGC'C
Cuts at: 0 74 1434 4110
Size: 74 1360 2676
Fragments arranged by size:
2676 1360 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 1042 1465 1786 1997 2194 3139 3591 4110
Size: 1042 423 321 211 197 945 452 519
Fragments arranged by size:
1042 945 519 452 423 321 211 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2307 2341 2478 2512 2658 2692 4110
Size: 2307 34 137 34 146 34 1418
Fragments arranged by size:
2307 1418 146 137 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 496 4110
Size: 496 3614
BclI T'GATC_A
Cuts at: 0 248 1100 4110
Size: 248 852 3010
Fragments arranged by size:
3010 852 248
BglI GCCn_nnn'nGGC
Cuts at: 0 1557 2919 4110
Size: 1557 1362 1191
Fragments arranged by size:
1557 1362 1191
BglII A'GATC_T
Cuts at: 0 1532 4110
Size: 1532 2578
BlpI GC'TnA_GC
Cuts at: 0 1136 4110
Size: 1136 2974
Bme1580I G_kGCm'C
Cuts at: 0 985 1874 2288 2330 3947 4110
Size: 985 889 414 42 1617 163
Fragments arranged by size:
1617 985 889 414 163 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 76 1755 4110
Size: 76 1679 2355
Fragments arranged by size:
2355 1679 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 174 963 2193 4110
Size: 174 789 1230 1917
Fragments arranged by size:
1917 1230 789 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1341 2188 3835 3934 4110
Size: 169 1172 847 1647 99 176
Fragments arranged by size:
1647 1172 847 176 169 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1732 3817 4110
Size: 367 1365 2085 293
Fragments arranged by size:
2085 1365 367 293
BsaI GGTCTCn'nnnn_
Cuts at: 0 215 790 1246 1568 1750 2060 3109 3284
Size: 215 575 456 322 182 310 1049 175
Cuts at: 3284 4110
Size: 826
Fragments arranged by size:
1049 826 575 456 322 310 215 182
175
BsaAI yAC'GTr
Cuts at: 0 2793 2997 3219 4110
Size: 2793 204 222 891
Fragments arranged by size:
2793 891 222 204
BsaBI GATnn'nnATC
Cuts at: 0 412 4110
Size: 412 3698
BsaHI Gr'CG_yC
Cuts at: 0 71 1054 1431 2810 4110
Size: 71 983 377 1379 1300
Fragments arranged by size:
1379 1300 983 377 71
BsaWI w'CCGG_w
Cuts at: 0 119 1226 2337 3533 3576 4110
Size: 119 1107 1111 1196 43 534
Fragments arranged by size:
1196 1111 1107 534 119 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2040 2070 2164 2194 3907 3937 4110
Size: 2040 30 94 30 1713 30 173
Fragments arranged by size:
2040 1713 173 94 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 553 1130 1262 2185 2356 2659 3898 3922
Size: 553 577 132 923 171 303 1239 24
Cuts at: 3922 3991 4110
Size: 69 119
Fragments arranged by size:
1239 923 577 553 303 171 132 119
69 24
BseYI C'CCAG_C
Cuts at: 0 2925 3048 3850 4008 4110
Size: 2925 123 802 158 102
Fragments arranged by size:
2925 802 158 123 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 666 850 2191 2484 3829 4110
Size: 666 184 1341 293 1345 281
Fragments arranged by size:
1345 1341 666 293 281 184
BsiEI CG_ry'CG
Cuts at: 0 32 2117 4110
Size: 32 2085 1993
Fragments arranged by size:
2085 1993 32
BsiHKAI G_wGCw'C
Cuts at: 0 455 985 1070 2348 4110
Size: 455 530 85 1278 1762
Fragments arranged by size:
1762 1278 530 455 85
BsmI GAATG_Cn'
Cuts at: 0 2128 4110
Size: 2128 1982
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2667 4110
Size: 2667 1443
Bsp1286I G_dGCh'C
Cuts at: 0 287 455 985 1070 1874 2288 2330 2348
Size: 287 168 530 85 804 414 42 18
Cuts at: 2348 2594 3061 3947 4110
Size: 246 467 886 163
Fragments arranged by size:
886 804 530 467 414 287 246 168
163 85 42 18
BspEI T'CCGG_A
Cuts at: 0 119 1226 2337 4110
Size: 119 1107 1111 1773
Fragments arranged by size:
1773 1111 1107 119
BspHI T'CATG_A
Cuts at: 0 245 3194 4110
Size: 245 2949 916
Fragments arranged by size:
2949 916 245
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 776 808 872 2213 2237 3217 3836 3851
Size: 776 32 64 1341 24 980 619 15
Cuts at: 3851 4110
Size: 259
Fragments arranged by size:
1341 980 776 619 259 64 32 24
15
BsrBI CCG'CTC
Cuts at: 0 3165 4110
Size: 3165 945
BsrDI GCAATG_nn'
Cuts at: 0 1448 3090 4110
Size: 1448 1642 1020
Fragments arranged by size:
1642 1448 1020
BsrFI r'CCGG_y
Cuts at: 0 14 2116 4110
Size: 14 2102 1994
Fragments arranged by size:
2102 1994 14
BssSI C'ACGA_G
Cuts at: 0 2331 4110
Size: 2331 1779
BstAPI GCAn_nnn'nTGC
Cuts at: 0 989 3391 4110
Size: 989 2402 719
Fragments arranged by size:
2402 989 719
BstEII G'GTnAC_C
Cuts at: 0 1788 4110
Size: 1788 2322
BstXI CCAn_nnnn'nTGG
Cuts at: 0 756 4110
Size: 756 3354
BstYI r'GATC_y
Cuts at: 0 1532 1878 2153 4110
Size: 1532 346 275 1957
Fragments arranged by size:
1957 1532 346 275
Bsu36I CC'TnA_GG
Cuts at: 0 1303 1693 2453 4110
Size: 1303 390 760 1657
Fragments arranged by size:
1657 1303 760 390
BtgI C'CryG_G
Cuts at: 0 74 497 2288 4110
Size: 74 423 1791 1822
Fragments arranged by size:
1822 1791 423 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2273 4110
Size: 2273 1837
BtsI GCAGTG_nn'
Cuts at: 0 639 2472 4110
Size: 639 1833 1638
Fragments arranged by size:
1833 1638 639
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2733 2768 3245 3280 4110
Size: 2733 35 477 35 830
Fragments arranged by size:
2733 830 477 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 752 2214 3222 4110
Size: 752 1462 1008 888
Fragments arranged by size:
1462 1008 888 752
EaeI y'GGCC_r
Cuts at: 0 2 29 53 464 2114 2279 3319 3342
Size: 2 27 24 411 1650 165 1040 23
Cuts at: 3342 4110
Size: 768
Fragments arranged by size:
1650 1040 768 411 165 27 24 23
2
EagI C'GGCC_G
Cuts at: 0 29 2114 4110
Size: 29 2085 1996
Fragments arranged by size:
2085 1996 29
EarI CTCTTCn'nnn_
Cuts at: 0 274 1363 2869 3292 4110
Size: 274 1089 1506 423 818
Fragments arranged by size:
1506 1089 818 423 274
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1180 2002 4092 4110
Size: 1180 822 2090 18
Fragments arranged by size:
2090 1180 822 18
EcoICRI GAG'CTC
Cuts at: 0 453 1068 4110
Size: 453 615 3042
Fragments arranged by size:
3042 615 453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 316 831 1341 1477 1939 2053 2093
Size: 169 147 515 510 136 462 114 40
Cuts at: 2093 2179 2188 2522 2850 3316 3607 3835 3934
Size: 86 9 334 328 466 291 228 99
Cuts at: 3934 4110
Size: 176
Fragments arranged by size:
515 510 466 462 334 328 291 228
176 169 147 136 114 99 86 40
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2181 3181 3674 3832 4110
Size: 2181 1000 493 158 278
Fragments arranged by size:
2181 1000 493 278 158
EcoO109I rG'GnC_Cy
Cuts at: 0 300 861 997 1608 2048 2249 3438 4001
Size: 300 561 136 611 440 201 1189 563
Cuts at: 4001 4110
Size: 109
Fragments arranged by size:
1189 611 563 561 440 300 201 136
109
EcoRI G'AATT_C
Cuts at: 0 963 4110
Size: 963 3147
EcoRV GAT'ATC
Cuts at: 0 117 4110
Size: 117 3993
FspI TGC'GCA
Cuts at: 0 3772 4110
Size: 3772 338
FspAI rTGC'GCAy
Cuts at: 0 3772 4110
Size: 3772 338
HaeII r_GCGC'y
Cuts at: 0 74 837 1434 4110
Size: 74 763 597 2676
Fragments arranged by size:
2676 763 597 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 621 653 1067 1099 1462 1494 1696 1728
Size: 621 32 414 32 363 32 202 32
Cuts at: 1728 1760 1870 1902 2040 2072 2133 2165 2800
Size: 32 110 32 138 32 61 32 635
Cuts at: 2800 2832 3014 3046 3607 3639 3943 3975 3985
Size: 32 182 32 561 32 304 32 10
Cuts at: 3985 4017 4077 4109 4110
Size: 32 60 32 1
Fragments arranged by size:
635 621 561 414 363 304 202 182
138 110 61 60 32 32 32 32
32 32 32 32 32 32 32 32
32 32 10 1
HincII GTy'rAC
Cuts at: 0 3141 3825 4110
Size: 3141 684 285
Fragments arranged by size:
3141 684 285
KasI G'GCGC_C
Cuts at: 0 70 1430 4110
Size: 70 1360 2680
Fragments arranged by size:
2680 1360 70
KpnI G_GTAC'C
Cuts at: 0 566 4110
Size: 566 3544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1645 1682 1724 1961 4110
Size: 607 1038 37 42 237 2149
Fragments arranged by size:
2149 1038 607 237 42 37
MscI TGG'CCA
Cuts at: 0 4 466 4110
Size: 4 462 3644
Fragments arranged by size:
3644 462 4
MslI CAynn'nnrTG
Cuts at: 0 1774 3772 4051 4110
Size: 1774 1998 279 59
Fragments arranged by size:
1998 1774 279 59
MspA1I CmG'CkG
Cuts at: 0 39 76 1135 2925 4110
Size: 39 37 1059 1790 1185
Fragments arranged by size:
1790 1185 1059 39 37
NaeI GCC'GGC
Cuts at: 0 16 2118 4110
Size: 16 2102 1992
Fragments arranged by size:
2102 1992 16
NarI GG'CG_CC
Cuts at: 0 71 1431 4110
Size: 71 1360 2679
Fragments arranged by size:
2679 1360 71
NgoMIV G'CCGG_C
Cuts at: 0 14 2116 4110
Size: 14 2102 1994
Fragments arranged by size:
2102 1994 14
NotI GC'GGCC_GC
Cuts at: 0 29 4110
Size: 29 4081
NspI r_CATG'y
Cuts at: 0 3621 3777 4110
Size: 3621 156 333
Fragments arranged by size:
3621 333 156
PasI CC'CwG_GG
Cuts at: 0 1726 4110
Size: 1726 2384
PciI A'CATG_T
Cuts at: 0 3617 4110
Size: 3617 493
PflMI CCAn_nnn'nTGG
Cuts at: 0 1404 1664 1965 3219 4110
Size: 1404 260 301 1254 891
Fragments arranged by size:
1404 1254 891 301 260
PfoI T'CCnGG_A
Cuts at: 0 488 824 3812 4110
Size: 488 336 2988 298
Fragments arranged by size:
2988 488 336 298
PmlI CAC'GTG
Cuts at: 0 3219 4110
Size: 3219 891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 380 412 3853 3885 4110
Size: 380 32 3441 32 225
Fragments arranged by size:
3441 380 225 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 300 861 997 2048 2249 3438 4001 4110
Size: 300 561 136 1051 201 1189 563 109
Fragments arranged by size:
1189 1051 563 561 300 201 136 109
PshAI GACnn'nnGTC
Cuts at: 0 554 1786 3743 4110
Size: 554 1232 1957 367
Fragments arranged by size:
1957 1232 554 367
PspXI vC'TCGA_Gb
Cuts at: 0 2043 4110
Size: 2043 2067
PstI C_TGCA'G
Cuts at: 0 2432 2698 4110
Size: 2432 266 1412
Fragments arranged by size:
2432 1412 266
PvuII CAG'CTG
Cuts at: 0 1135 4110
Size: 1135 2975
SacI G_AGCT'C
Cuts at: 0 455 1070 4110
Size: 455 615 3040
Fragments arranged by size:
3040 615 455
SacII CC_GC'GG
Cuts at: 0 77 4110
Size: 77 4033
SanDI GG'GwC_CC
Cuts at: 0 997 4110
Size: 997 3113
SapI GCTCTTCn'nnn_
Cuts at: 0 274 3292 4110
Size: 274 3018 818
Fragments arranged by size:
3018 818 274
SexAI A'CCwGG_T
Cuts at: 0 1964 2050 4110
Size: 1964 86 2060
Fragments arranged by size:
2060 1964 86
SfcI C'TryA_G
Cuts at: 0 2428 2694 2724 4110
Size: 2428 266 30 1386
Fragments arranged by size:
2428 1386 266 30
SfoI GGC'GCC
Cuts at: 0 72 1432 4110
Size: 72 1360 2678
Fragments arranged by size:
2678 1360 72
SmaI CCC'GGG
Cuts at: 0 421 4110
Size: 421 3689
SmlI C'TyrA_G
Cuts at: 0 286 382 1747 2043 3020 3832 4110
Size: 286 96 1365 296 977 812 278
Fragments arranged by size:
1365 977 812 296 286 278 96
SspI AAT'ATT
Cuts at: 0 2823 4110
Size: 2823 1287
StuI AGG'CCT
Cuts at: 0 695 2352 3187 3680 4110
Size: 695 1657 835 493 430
Fragments arranged by size:
1657 835 695 493 430
StyI C'CwwG_G
Cuts at: 0 1007 1256 1286 1424 2253 2507 3245 3675
Size: 1007 249 30 138 829 254 738 430
Cuts at: 3675 4110
Size: 435
Fragments arranged by size:
1007 829 738 435 430 254 249 138
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1739 1972 3180 4085 4110
Size: 1739 233 1208 905 25
Fragments arranged by size:
1739 1208 905 233 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 772 804 4110
Size: 772 32 3306
Fragments arranged by size:
3306 772 32
Tth111I GACn'n_nGTC
Cuts at: 0 2052 4110
Size: 2052 2058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 753 4110
Size: 753 3357
XhoI C'TCGA_G
Cuts at: 0 286 2043 3020 4110
Size: 286 1757 977 1090
Fragments arranged by size:
1757 1090 977 286
XmaI C'CCGG_G
Cuts at: 0 419 4110
Size: 419 3691
XmnI GAAnn'nnTTC
Cuts at: 0 1357 3202 4110
Size: 1357 1845 908
Fragments arranged by size:
1845 1357 908
ZraI GAC'GTC
Cuts at: 0 1055 2811 4110
Size: 1055 1756 1299
Fragments arranged by size:
1756 1299 1055
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AhdI AlfI
AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspXI PstI PvuII
SacI SacII SanDI SapI SexAI SfcI SfoI SmaI
SmlI SspI StuI StyI TaqII TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AleI AloI ApaI AscI
AseI AsiSI AvrII BaeI BbvCI BfrBI BmgBI BmtI
Bpu10I BsiWI BsrGI BssHII BstBI BstZ17I ClaI DraI
DrdI FalI FseI HindIII HpaI MfeI MluI NcoI
NdeI NheI NruI NsiI PacI PmeI PsiI PspOMI
PsrI PvuI RsrII SalI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI TatI XbaI