Restriction map for
pFN21AE3454
(Linear) (Six-base) MAPSORT of: KIEE3454.seq Check: 7203 from: 1 to: 3984
KIEE3454.orf
With 182 enzymes: SgfI *
July 3, 2012 17:53 ..
AccI GT'mk_AC
Cuts at: 0 207 2452 3224 3984
Size: 207 2245 772 760
Fragments arranged by size:
2245 772 760 207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 590 1097 1688 2297 2537 3185 3976
Size: 568 22 507 591 609 240 648 791
Cuts at: 3976 3984
Size: 8
Fragments arranged by size:
791 648 609 591 568 507 240 22
8
AflII C'TTAA_G
Cuts at: 0 1930 2835 3670 3984
Size: 1930 905 835 314
Fragments arranged by size:
1930 905 835 314
AflIII A'CryG_T
Cuts at: 0 863 2045 3357 3984
Size: 863 1182 1312 627
Fragments arranged by size:
1312 1182 863 627
AlwNI CAG_nnn'CTG
Cuts at: 0 751 2989 3954 3984
Size: 751 2238 965 30
Fragments arranged by size:
2238 965 751 30
ApoI r'AATT_y
Cuts at: 0 1002 1593 1650 1715 2345 2398 2586 2654
Size: 1002 591 57 65 630 53 188 68
Cuts at: 2654 2712 2847 3112 3984
Size: 58 135 265 872
Fragments arranged by size:
1002 872 630 591 265 188 135 68
65 58 57 53
AvrII C'CTAG_G
Cuts at: 0 1762 3984
Size: 1762 2222
BamHI G'GATC_C
Cuts at: 0 819 3984
Size: 819 3165
BanI G'GyrC_C
Cuts at: 0 1443 3652 3984
Size: 1443 2209 332
Fragments arranged by size:
2209 1443 332
BanII G_rGCy'C
Cuts at: 0 392 455 773 801 3089 3984
Size: 392 63 318 28 2288 895
Fragments arranged by size:
2288 895 392 318 63 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 302 827 1645 2303 2564 3984
Size: 302 525 818 658 261 1420
Fragments arranged by size:
1420 818 658 525 302 261
BciVI GTATCCnnnnn_n'
Cuts at: 0 3273 3984
Size: 3273 711
BfrBI ATG'CAT
Cuts at: 0 1692 3681 3984
Size: 1692 1989 303
Fragments arranged by size:
1989 1692 303
BglII A'GATC_T
Cuts at: 0 487 1926 2505 3984
Size: 487 1439 579 1479
Fragments arranged by size:
1479 1439 579 487
BlpI GC'TnA_GC
Cuts at: 0 772 3984
Size: 772 3212
Bme1580I G_kGCm'C
Cuts at: 0 345 2576 3984
Size: 345 2231 1408
Fragments arranged by size:
2231 1408 345
BmrI ACTGGGnnnn_n'
Cuts at: 0 191 2570 3984
Size: 191 2379 1414
Fragments arranged by size:
2379 1414 191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 3771 3931 3984
Size: 925 2846 160 53
Fragments arranged by size:
2846 925 160 53
Bpu10I CC'TnA_GC
Cuts at: 0 391 737 2375 3088 3984
Size: 391 346 1638 713 896
Fragments arranged by size:
1638 896 713 391 346
BsaI GGTCTCn'nnnn_
Cuts at: 0 3238 3984
Size: 3238 746
BsaAI yAC'GTr
Cuts at: 0 2048 3984
Size: 2048 1936
BsaWI w'CCGG_w
Cuts at: 0 1811 3314 3984
Size: 1811 1503 670
Fragments arranged by size:
1811 1503 670
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 788 818 1496 1526 3009 3039 3744 3774
Size: 788 30 678 30 1483 30 705 30
Cuts at: 3774 3984
Size: 210
Fragments arranged by size:
1483 788 705 678 210 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 527 3121 3984
Size: 151 376 2594 863
Fragments arranged by size:
2594 863 376 151
BseYI C'CCAG_C
Cuts at: 0 1190 2564 3984
Size: 1190 1374 1420
Fragments arranged by size:
1420 1374 1190
BsiHKAI G_wGCw'C
Cuts at: 0 392 455 773 871 2207 3843 3984
Size: 392 63 318 98 1336 1636 141
Fragments arranged by size:
1636 1336 392 318 141 98 63
BsmI GAATG_Cn'
Cuts at: 0 1194 3984
Size: 1194 2790
Bsp1286I G_dGCh'C
Cuts at: 0 345 392 455 773 801 871 2207 2576
Size: 345 47 63 318 28 70 1336 369
Cuts at: 2576 3089 3843 3984
Size: 513 754 141
Fragments arranged by size:
1336 754 513 369 345 318 141 70
63 47 28
BspHI T'CATG_A
Cuts at: 0 54 2784 3984
Size: 54 2730 1200
Fragments arranged by size:
2730 1200 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3631 3984
Size: 3631 353
BsrDI GCAATG_nn'
Cuts at: 0 37 465 1286 3469 3984
Size: 37 428 821 2183 515
Fragments arranged by size:
2183 821 515 428 37
BsrGI T'GTAC_A
Cuts at: 0 1563 2244 2601 3984
Size: 1563 681 357 1383
Fragments arranged by size:
1563 1383 681 357
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2361 3630 3984
Size: 2361 1269 354
Fragments arranged by size:
2361 1269 354
BstYI r'GATC_y
Cuts at: 0 192 487 819 1146 1926 2505 2537 2985
Size: 192 295 332 327 780 579 32 448
Cuts at: 2985 3984
Size: 999
Fragments arranged by size:
999 780 579 448 332 327 295 192
32
BstZ17I GTA'TAC
Cuts at: 0 3225 3984
Size: 3225 759
BtgI C'CryG_G
Cuts at: 0 1053 3806 3984
Size: 1053 2753 178
Fragments arranged by size:
2753 1053 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 311 3535 3984
Size: 311 3224 449
Fragments arranged by size:
3224 449 311
BtsI GCAGTG_nn'
Cuts at: 0 2576 3984
Size: 2576 1408
DraI TTT'AAA
Cuts at: 0 3158 3647 3984
Size: 3158 489 337
Fragments arranged by size:
3158 489 337
DraIII CAC_nnn'GTG
Cuts at: 0 2116 3984
Size: 2116 1868
DrdI GACnn_nn'nnGTC
Cuts at: 0 831 3984
Size: 831 3153
EaeI y'GGCC_r
Cuts at: 0 603 3818 3984
Size: 603 3215 166
Fragments arranged by size:
3215 603 166
EarI CTCTTCn'nnn_
Cuts at: 0 72 566 1031 1076 1217 1298 2119 2441
Size: 72 494 465 45 141 81 821 322
Cuts at: 2441 2516 3984
Size: 75 1468
Fragments arranged by size:
1468 821 494 465 322 141 81 75
72 45
EcoICRI GAG'CTC
Cuts at: 0 390 453 771 3984
Size: 390 63 318 3213
Fragments arranged by size:
3213 390 318 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 590 925 1097 1688 2297 2537 3185
Size: 568 22 335 172 591 609 240 648
Cuts at: 3185 3771 3931 3976 3984
Size: 586 160 45 8
Fragments arranged by size:
648 609 591 586 568 335 240 172
160 45 22 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 907 3984
Size: 907 3077
EcoO109I rG'GnC_Cy
Cuts at: 0 1672 2000 3984
Size: 1672 328 1984
Fragments arranged by size:
1984 1672 328
EcoRI G'AATT_C
Cuts at: 0 1593 2398 2586 3984
Size: 1593 805 188 1398
Fragments arranged by size:
1593 1398 805 188
EcoRV GAT'ATC
Cuts at: 0 3558 3747 3984
Size: 3558 189 237
Fragments arranged by size:
3558 237 189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2762 3981 3984
Size: 2762 1219 3
Fragments arranged by size:
2762 1219 3
FspI TGC'GCA
Cuts at: 0 3904 3984
Size: 3904 80
FspAI rTGC'GCAy
Cuts at: 0 3904 3984
Size: 3904 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 644 676 873 905 1431 1463
Size: 49 32 563 32 197 32 526 32
Cuts at: 1463 1967 1999 2314 2346 2941 2973 3862 3894
Size: 504 32 315 32 595 32 889 32
Cuts at: 3894 3984
Size: 90
Fragments arranged by size:
889 595 563 526 504 315 197 90
49 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1554 3363 3984
Size: 1554 1809 621
Fragments arranged by size:
1809 1554 621
HindIII A'AGCT_T
Cuts at: 0 1323 3984
Size: 1323 2661
MfeI C'AATT_G
Cuts at: 0 1756 3984
Size: 1756 2228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 847 1592 3984
Size: 97 750 745 2392
Fragments arranged by size:
2392 750 745 97
MslI CAynn'nnrTG
Cuts at: 0 368 1271 3080 3415 3984
Size: 368 903 1809 335 569
Fragments arranged by size:
1809 903 569 368 335
MspA1I CmG'CkG
Cuts at: 0 2564 3984
Size: 2564 1420
NcoI C'CATG_G
Cuts at: 0 1053 3806 3984
Size: 1053 2753 178
Fragments arranged by size:
2753 1053 178
NdeI CA'TA_TG
Cuts at: 0 590 2165 3984
Size: 590 1575 1819
Fragments arranged by size:
1819 1575 590
NruI TCG'CGA
Cuts at: 0 3554 3984
Size: 3554 430
NsiI A_TGCA'T
Cuts at: 0 1694 3683 3984
Size: 1694 1989 301
Fragments arranged by size:
1989 1694 301
NspI r_CATG'y
Cuts at: 0 31 355 867 3361 3389 3669 3909 3984
Size: 31 324 512 2494 28 280 240 75
Fragments arranged by size:
2494 512 324 280 240 75 31 28
PciI A'CATG_T
Cuts at: 0 863 3357 3984
Size: 863 2494 627
Fragments arranged by size:
2494 863 627
PflMI CCAn_nnn'nTGG
Cuts at: 0 1751 2970 3984
Size: 1751 1219 1014
Fragments arranged by size:
1751 1219 1014
PfoI T'CCnGG_A
Cuts at: 0 792 1860 3984
Size: 792 1068 2124
Fragments arranged by size:
2124 1068 792
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3469 3501 3984
Size: 3469 32 483
Fragments arranged by size:
3469 483 32
PpuMI rG'GwC_Cy
Cuts at: 0 1672 2000 3984
Size: 1672 328 1984
Fragments arranged by size:
1984 1672 328
PshAI GACnn'nnGTC
Cuts at: 0 50 161 3984
Size: 50 111 3823
Fragments arranged by size:
3823 111 50
PsiI TTA'TAA
Cuts at: 0 3454 3984
Size: 3454 530
PstI C_TGCA'G
Cuts at: 0 163 1029 2925 3984
Size: 163 866 1896 1059
Fragments arranged by size:
1896 1059 866 163
PvuII CAG'CTG
Cuts at: 0 2564 3984
Size: 2564 1420
SacI G_AGCT'C
Cuts at: 0 392 455 773 3984
Size: 392 63 318 3211
Fragments arranged by size:
3211 392 318 63
SanDI GG'GwC_CC
Cuts at: 0 2000 3984
Size: 2000 1984
SapI GCTCTTCn'nnn_
Cuts at: 0 2119 2516 3984
Size: 2119 397 1468
Fragments arranged by size:
2119 1468 397
SfcI C'TryA_G
Cuts at: 0 159 1025 2921 3322 3782 3984
Size: 159 866 1896 401 460 202
Fragments arranged by size:
1896 866 460 401 202 159
SmlI C'TyrA_G
Cuts at: 0 1930 2835 3670 3984
Size: 1930 905 835 314
Fragments arranged by size:
1930 905 835 314
SpeI A'CTAG_T
Cuts at: 0 3238 3984
Size: 3238 746
SspI AAT'ATT
Cuts at: 0 1020 1296 2764 3984
Size: 1020 276 1468 1220
Fragments arranged by size:
1468 1220 1020 276
StuI AGG'CCT
Cuts at: 0 1749 3984
Size: 1749 2235
StyI C'CwwG_G
Cuts at: 0 929 1053 1484 1750 1762 2237 3185 3806
Size: 929 124 431 266 12 475 948 621
Cuts at: 3806 3984
Size: 178
Fragments arranged by size:
948 929 621 475 431 266 178 124
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3670 3984
Size: 3670 314
TatI w'GTAC_w
Cuts at: 0 596 1309 1314 1563 2244 2601 3763 3984
Size: 596 713 5 249 681 357 1162 221
Fragments arranged by size:
1162 713 681 596 357 249 221 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 219 251 1703 1735 3174 3206 3984
Size: 219 32 1452 32 1439 32 778
Fragments arranged by size:
1452 1439 778 219 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2670 3984
Size: 2670 1314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3813 3984
Size: 3813 171
XmnI GAAnn'nnTTC
Cuts at: 0 2398 3984
Size: 2398 1586
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApoI AvrII BamHI
BanI BanII BbsI BciVI BfrBI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI FspAI Hin4I HincII HindIII MfeI
MmeI MslI MspA1I NcoI NdeI NruI NsiI NspI
PciI PflMI PfoI PpiI PpuMI PshAI PsiI PstI
PvuII SacI SanDI SapI SfcI SmlI SpeI SspI
StuI StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
BaeI BbeI BbvCI BcgI BclI BglI BmgBI BmtI
BplI BpuEI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
Bsu36I ClaI CspCI EagI EciI FseI HaeII HpaI
KasI KpnI MluI MscI NaeI NarI NgoMIV NheI
NotI PacI PasI PmeI PmlI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI
Tth111I XhoI XmaI ZraI