Restriction map for
pFN21AE3449
(Linear) (Six-base) MAPSORT of: KIEE3449.seq Check: 2377 from: 1 to: 3933
KIEE3449.orf
With 182 enzymes: SgfI *
October 21, 2016 17:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 879 1451 3933
Size: 879 572 2482
Fragments arranged by size:
2482 879 572
AccI GT'mk_AC
Cuts at: 0 1355 1802 2642 3933
Size: 1355 447 840 1291
Fragments arranged by size:
1355 1291 840 447
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 1569 2885 3441 3673 3933
Size: 466 1103 1316 556 232 260
Fragments arranged by size:
1316 1103 556 466 260 232
AfeI AGC'GCT
Cuts at: 0 1289 1627 3003 3933
Size: 1289 338 1376 930
Fragments arranged by size:
1376 1289 930 338
AflIII A'CryG_T
Cuts at: 0 1610 1673 2066 3933
Size: 1610 63 393 1867
Fragments arranged by size:
1867 1610 393 63
AhdI GACnn_n'nnGTC
Cuts at: 0 2235 3894 3933
Size: 2235 1659 39
Fragments arranged by size:
2235 1659 39
AleI CACnn'nnGTG
Cuts at: 0 2038 3933
Size: 2038 1895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1489 3933
Size: 1489 2444
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3901 3933
Size: 3901 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2332 2413 2620 3353 3624 3933
Size: 2332 81 207 733 271 309
Fragments arranged by size:
2332 733 309 271 207 81
ApaI G_GGCC'C
Cuts at: 0 744 1057 1437 2750 2774 2861 3699 3933
Size: 744 313 380 1313 24 87 838 234
Fragments arranged by size:
1313 838 744 380 313 234 87 24
ApaLI G'TGCA_C
Cuts at: 0 928 1516 3820 3933
Size: 928 588 2304 113
Fragments arranged by size:
2304 928 588 113
ApoI r'AATT_y
Cuts at: 0 394 2044 2947 3933
Size: 394 1650 903 986
Fragments arranged by size:
1650 986 903 394
AvaI C'yCGr_G
Cuts at: 0 416 1031 1368 2172 3933
Size: 416 615 337 804 1761
Fragments arranged by size:
1761 804 615 416 337
BamHI G'GATC_C
Cuts at: 0 2497 3933
Size: 2497 1436
BanI G'GyrC_C
Cuts at: 0 3382 3933
Size: 3382 551
BanII G_rGCy'C
Cuts at: 0 374 636 744 992 1057 1437 1700 2132
Size: 374 262 108 248 65 380 263 432
Cuts at: 2132 2546 2750 2774 2861 3062 3699 3919 3933
Size: 414 204 24 87 201 637 220 14
Fragments arranged by size:
637 432 414 380 374 263 262 248
220 204 201 108 87 65 24 14
BbeI G_GCGC'C
Cuts at: 0 3386 3933
Size: 3386 547
BbsI GAAGACnn'nnnn_
Cuts at: 0 852 2639 2891 3933
Size: 852 1787 252 1042
Fragments arranged by size:
1787 1042 852 252
BbvCI CC'TCA_GC
Cuts at: 0 3265 3933
Size: 3265 668
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3346 3380 3817 3851 3933
Size: 3346 34 437 34 82
Fragments arranged by size:
3346 437 82 34 34
BfrBI ATG'CAT
Cuts at: 0 199 3933
Size: 199 3734
BglI GCCn_nnn'nGGC
Cuts at: 0 1061 1637 1975 2704 3281 3933
Size: 1061 576 338 729 577 652
Fragments arranged by size:
1061 729 652 577 576 338
BglII A'GATC_T
Cuts at: 0 449 3543 3933
Size: 449 3094 390
Fragments arranged by size:
3094 449 390
BlpI GC'TnA_GC
Cuts at: 0 2731 2788 3933
Size: 2731 57 1145
Fragments arranged by size:
2731 1145 57
Bme1580I G_kGCm'C
Cuts at: 0 744 932 1057 1346 1437 1520 2750 2774
Size: 744 188 125 289 91 83 1230 24
Cuts at: 2774 2861 3699 3824 3933
Size: 87 838 125 109
Fragments arranged by size:
1230 838 744 289 188 125 125 109
91 87 83 24
BmgBI CAC'GTC
Cuts at: 0 464 1917 3933
Size: 464 1453 2016
Fragments arranged by size:
2016 1453 464
BmrI ACTGGGnnnn_n'
Cuts at: 0 112 3933
Size: 112 3821
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1936 2778 3933
Size: 1936 842 1155
Fragments arranged by size:
1936 1155 842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 1015 1371 2493 2697 3203 3555 3933
Size: 636 379 356 1122 204 506 352 378
Fragments arranged by size:
1122 636 506 379 378 356 352 204
Bpu10I CC'TnA_GC
Cuts at: 0 2830 3265 3933
Size: 2830 435 668
Fragments arranged by size:
2830 668 435
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 505 1150 3631 3933
Size: 155 350 645 2481 302
Fragments arranged by size:
2481 645 350 302 155
BsaI GGTCTCn'nnnn_
Cuts at: 0 1410 3933
Size: 1410 2523
BsaAI yAC'GTr
Cuts at: 0 1806 3933
Size: 1806 2127
BsaHI Gr'CG_yC
Cuts at: 0 1898 2360 3383 3748 3890 3933
Size: 1898 462 1023 365 142 43
Fragments arranged by size:
1898 1023 462 365 142 43
BsaWI w'CCGG_w
Cuts at: 0 3777 3933
Size: 3777 156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1162 1192 1369 1390 1399 1420
Size: 895 30 237 30 177 21 9 21
Cuts at: 1420 2431 2461 3253 3283 3901 3931 3933
Size: 1011 30 792 30 618 30 2
Fragments arranged by size:
1011 895 792 618 237 177 30 30
30 30 30 21 21 9 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 916 1167 1708 1788 2233 2867 3493
Size: 343 573 251 541 80 445 634 626
Cuts at: 3493 3558 3933
Size: 65 375
Fragments arranged by size:
634 626 573 541 445 375 343 251
80 65
BseYI C'CCAG_C
Cuts at: 0 1266 1418 2296 2915 3061 3099 3499 3933
Size: 1266 152 878 619 146 38 400 434
Fragments arranged by size:
1266 878 619 434 400 152 146 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1429 2359 2438 2590 2641 2857 3019
Size: 496 933 930 79 152 51 216 162
Cuts at: 3019 3408 3697 3933
Size: 389 289 236
Fragments arranged by size:
933 930 496 389 289 236 216 162
152 79 51
BsiEI CG_ry'CG
Cuts at: 0 66 719 953 1959 3370 3933
Size: 66 653 234 1006 1411 563
Fragments arranged by size:
1411 1006 653 563 234 66
BsiHKAI G_wGCw'C
Cuts at: 0 374 932 992 1520 1922 2845 3824 3933
Size: 374 558 60 528 402 923 979 109
Fragments arranged by size:
979 923 558 528 402 374 109 60
BsiWI C'GTAC_G
Cuts at: 0 3848 3933
Size: 3848 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1585 1907 2462 3933
Size: 1585 322 555 1471
Fragments arranged by size:
1585 1471 555 322
Bsp1286I G_dGCh'C
Cuts at: 0 374 636 744 932 992 1057 1346 1437
Size: 374 262 108 188 60 65 289 91
Cuts at: 1437 1520 1700 1922 2132 2546 2750 2774 2845
Size: 83 180 222 210 414 204 24 71
Cuts at: 2845 2861 3062 3699 3824 3919 3933
Size: 16 201 637 125 95 14
Fragments arranged by size:
637 414 374 289 262 222 210 204
201 188 180 125 108 95 91 83
71 65 60 24 16 14
BspEI T'CCGG_A
Cuts at: 0 3777 3933
Size: 3777 156
BspHI T'CATG_A
Cuts at: 0 263 1742 3933
Size: 263 1479 2191
Fragments arranged by size:
2191 1479 263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 770 879 1451 2735 3104 3933
Size: 770 109 572 1284 369 829
Fragments arranged by size:
1284 829 770 572 369 109
BsrBI CCG'CTC
Cuts at: 0 906 1149 1778 3483 3684 3933
Size: 906 243 629 1705 201 249
Fragments arranged by size:
1705 906 629 249 243 201
BsrDI GCAATG_nn'
Cuts at: 0 3599 3933
Size: 3599 334
BsrFI r'CCGG_y
Cuts at: 0 488 1379 1958 2601 3192 3933
Size: 488 891 579 643 591 741
Fragments arranged by size:
891 741 643 591 579 488
BsrGI T'GTAC_A
Cuts at: 0 1454 1607 3933
Size: 1454 153 2326
Fragments arranged by size:
2326 1454 153
BssSI C'ACGA_G
Cuts at: 0 654 3933
Size: 654 3279
BstAPI GCAn_nnn'nTGC
Cuts at: 0 483 936 2425 3720 3933
Size: 483 453 1489 1295 213
Fragments arranged by size:
1489 1295 483 453 213
BstBI TT'CG_AA
Cuts at: 0 807 3933
Size: 807 3126
BstEII G'GTnAC_C
Cuts at: 0 1216 3933
Size: 1216 2717
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1111 1643 3933
Size: 1111 532 2290
Fragments arranged by size:
2290 1111 532
BstYI r'GATC_y
Cuts at: 0 449 2497 2781 3045 3543 3933
Size: 449 2048 284 264 498 390
Fragments arranged by size:
2048 498 449 390 284 264
Bsu36I CC'TnA_GG
Cuts at: 0 2753 3398 3933
Size: 2753 645 535
Fragments arranged by size:
2753 645 535
BtgI C'CryG_G
Cuts at: 0 1049 1592 1736 2161 2279 3752 3933
Size: 1049 543 144 425 118 1473 181
Fragments arranged by size:
1473 1049 543 425 181 144 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 900 3933
Size: 900 3033
BtsI GCAGTG_nn'
Cuts at: 0 585 1071 1259 1518 3146 3933
Size: 585 486 188 259 1628 787
Fragments arranged by size:
1628 787 585 486 259 188
DraIII CAC_nnn'GTG
Cuts at: 0 3720 3933
Size: 3720 213
DrdI GACnn_nn'nnGTC
Cuts at: 0 3078 3132 3933
Size: 3078 54 801
Fragments arranged by size:
3078 801 54
EaeI y'GGCC_r
Cuts at: 0 63 137 279 950 1550 1645 1823 1956
Size: 63 74 142 671 600 95 178 133
Cuts at: 1956 3326 3367 3761 3815 3933
Size: 1370 41 394 54 118
Fragments arranged by size:
1370 671 600 394 178 142 133 118
95 74 63 54 41
EagI C'GGCC_G
Cuts at: 0 63 950 1956 3367 3933
Size: 63 887 1006 1411 566
Fragments arranged by size:
1411 1006 887 566 63
EarI CTCTTCn'nnn_
Cuts at: 0 218 641 1159 1588 2390 2436 2944 3178
Size: 218 423 518 429 802 46 508 234
Cuts at: 3178 3933
Size: 755
Fragments arranged by size:
802 755 518 508 429 423 234 218
46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1108 1705 2996 3933
Size: 1108 597 1291 937
Fragments arranged by size:
1291 1108 937 597
EcoICRI GAG'CTC
Cuts at: 0 372 990 3933
Size: 372 618 2943
Fragments arranged by size:
2943 618 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 636 1015 1371 1569 2493 2697 2885
Size: 466 170 379 356 198 924 204 188
Cuts at: 2885 3203 3441 3555 3673 3933
Size: 318 238 114 118 260
Fragments arranged by size:
924 466 379 356 318 260 238 204
198 188 170 118 114
EcoNI CCTnn'n_nnAGG
Cuts at: 0 640 2458 3933
Size: 640 1818 1475
Fragments arranged by size:
1818 1475 640
EcoO109I rG'GnC_Cy
Cuts at: 0 132 421 516 741 1054 1373 1434 1787
Size: 132 289 95 225 313 319 61 353
Cuts at: 1787 2306 2706 2747 2770 2771 2796 2857 2858
Size: 519 400 41 23 1 25 61 1
Cuts at: 2858 3166 3621 3696 3933
Size: 308 455 75 237
Fragments arranged by size:
519 455 400 353 319 313 308 289
237 225 132 95 75 61 61 41
25 23 1 1
EcoRV GAT'ATC
Cuts at: 0 735 3933
Size: 735 3198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3888 3933
Size: 3888 45
HaeII r_GCGC'y
Cuts at: 0 22 623 1022 1090 1291 1629 3005 3026
Size: 22 601 399 68 201 338 1376 21
Cuts at: 3026 3386 3933
Size: 360 547
Fragments arranged by size:
1376 601 547 399 360 338 201 68
22 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 895 927 1162 1194 2221 2253 2320 2352
Size: 895 32 235 32 1027 32 67 32
Cuts at: 2352 2431 2463 2489 2521 3213 3245 3253 3285
Size: 79 32 26 32 692 32 8 32
Cuts at: 3285 3535 3567 3880 3912 3933
Size: 250 32 313 32 21
Fragments arranged by size:
1027 895 692 313 250 235 79 67
32 32 32 32 32 32 32 32
32 32 26 21 8
HincII GTy'rAC
Cuts at: 0 2643 3933
Size: 2643 1290
HindIII A'AGCT_T
Cuts at: 0 271 3933
Size: 271 3662
KasI G'GCGC_C
Cuts at: 0 3382 3933
Size: 3382 551
MluI A'CGCG_T
Cuts at: 0 2066 3933
Size: 2066 1867
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 3878 3933
Size: 731 3147 55
Fragments arranged by size:
3147 731 55
MscI TGG'CCA
Cuts at: 0 1647 1825 3933
Size: 1647 178 2108
Fragments arranged by size:
2108 1647 178
MslI CAynn'nnrTG
Cuts at: 0 1726 1987 2038 3933
Size: 1726 261 51 1895
Fragments arranged by size:
1895 1726 261 51
MspA1I CmG'CkG
Cuts at: 0 387 614 936 1060 1594 1758 2683 2735
Size: 387 227 322 124 534 164 925 52
Cuts at: 2735 2802 2869 3218 3292 3743 3933
Size: 67 67 349 74 451 190
Fragments arranged by size:
925 534 451 387 349 322 227 190
164 124 74 67 67 52
NaeI GCC'GGC
Cuts at: 0 1960 3194 3933
Size: 1960 1234 739
Fragments arranged by size:
1960 1234 739
NarI GG'CG_CC
Cuts at: 0 3383 3933
Size: 3383 550
NcoI C'CATG_G
Cuts at: 0 2161 3933
Size: 2161 1772
NgoMIV G'CCGG_C
Cuts at: 0 1958 3192 3933
Size: 1958 1234 741
Fragments arranged by size:
1958 1234 741
NotI GC'GGCC_GC
Cuts at: 0 950 3933
Size: 950 2983
NsiI A_TGCA'T
Cuts at: 0 201 3933
Size: 201 3732
NspI r_CATG'y
Cuts at: 0 486 1476 1614 1677 3933
Size: 486 990 138 63 2256
Fragments arranged by size:
2256 990 486 138 63
PasI CC'CwG_GG
Cuts at: 0 48 744 1339 1893 2132 2528 2766 3378
Size: 48 696 595 554 239 396 238 612
Cuts at: 3378 3933
Size: 555
Fragments arranged by size:
696 612 595 555 554 396 239 238
48
PciI A'CATG_T
Cuts at: 0 1610 1673 3933
Size: 1610 63 2260
Fragments arranged by size:
2260 1610 63
PfoI T'CCnGG_A
Cuts at: 0 992 1121 1322 3180 3659 3933
Size: 992 129 201 1858 479 274
Fragments arranged by size:
1858 992 479 274 201 129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3901 3933
Size: 3901 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 516 1373 2306 2796 3621 3933
Size: 132 384 857 933 490 825 312
Fragments arranged by size:
933 857 825 490 384 312 132
PshAI GACnn'nnGTC
Cuts at: 0 2363 3933
Size: 2363 1570
PspOMI G'GGCC_C
Cuts at: 0 740 1053 1433 2746 2770 2857 3695 3933
Size: 740 313 380 1313 24 87 838 238
Fragments arranged by size:
1313 838 740 380 313 238 87 24
PspXI vC'TCGA_Gb
Cuts at: 0 2172 3933
Size: 2172 1761
PstI C_TGCA'G
Cuts at: 0 14 312 890 941 1468 1786 1967 3022
Size: 14 298 578 51 527 318 181 1055
Cuts at: 3022 3132 3933
Size: 110 801
Fragments arranged by size:
1055 801 578 527 318 298 181 110
51 14
PvuI CG_AT'CG
Cuts at: 0 719 3933
Size: 719 3214
PvuII CAG'CTG
Cuts at: 0 387 614 936 2683 2735 3218 3292 3933
Size: 387 227 322 1747 52 483 74 641
Fragments arranged by size:
1747 641 483 387 322 227 74 52
SacI G_AGCT'C
Cuts at: 0 374 992 3933
Size: 374 618 2941
Fragments arranged by size:
2941 618 374
SacII CC_GC'GG
Cuts at: 0 1595 3933
Size: 1595 2338
SalI G'TCGA_C
Cuts at: 0 2641 3933
Size: 2641 1292
SanDI GG'GwC_CC
Cuts at: 0 2306 3933
Size: 2306 1627
SapI GCTCTTCn'nnn_
Cuts at: 0 1159 3933
Size: 1159 2774
ScaI AGT'ACT
Cuts at: 0 826 3933
Size: 826 3107
SexAI A'CCwGG_T
Cuts at: 0 3767 3933
Size: 3767 166
SfcI C'TryA_G
Cuts at: 0 10 308 317 609 886 937 1071 1464
Size: 10 298 9 292 277 51 134 393
Cuts at: 1464 1782 1963 3018 3128 3933
Size: 318 181 1055 110 805
Fragments arranged by size:
1055 805 393 318 298 292 277 181
134 110 51 10 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2704 3933
Size: 2704 1229
SfoI GGC'GCC
Cuts at: 0 3384 3933
Size: 3384 549
SmlI C'TyrA_G
Cuts at: 0 170 416 520 1165 2172 3646 3933
Size: 170 246 104 645 1007 1474 287
Fragments arranged by size:
1474 1007 645 287 246 170 104
StuI AGG'CCT
Cuts at: 0 1082 2698 3438 3933
Size: 1082 1616 740 495
Fragments arranged by size:
1616 1082 740 495
StyI C'CwwG_G
Cuts at: 0 549 1181 2161 2435 2742 3255 3279 3433
Size: 549 632 980 274 307 513 24 154
Cuts at: 3433 3933
Size: 500
Fragments arranged by size:
980 632 549 513 500 307 274 154
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1352 3136 3513 3933
Size: 1352 1784 377 420
Fragments arranged by size:
1784 1352 420 377
TatI w'GTAC_w
Cuts at: 0 824 851 1454 1607 1670 3933
Size: 824 27 603 153 63 2263
Fragments arranged by size:
2263 824 603 153 63 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1927 1959 2969 3001 3933
Size: 1927 32 1010 32 932
Fragments arranged by size:
1927 1010 932 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2638 3225 3933
Size: 2638 587 708
Fragments arranged by size:
2638 708 587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 921 1640 2058 3933
Size: 921 719 418 1875
Fragments arranged by size:
1875 921 719 418
XhoI C'TCGA_G
Cuts at: 0 416 2172 3933
Size: 416 1756 1761
Fragments arranged by size:
1761 1756 416
XmnI GAAnn'nnTTC
Cuts at: 0 451 1990 3933
Size: 451 1539 1943
Fragments arranged by size:
1943 1539 451
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII KasI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NsiI NspI PasI PciI PfoI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI ScaI SexAI SfcI SfiI SfoI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AscI AseI AsiSI
AvrII BaeI BciVI BclI BmtI BsaBI BsmI BssHII
BstZ17I ClaI CspCI DraI EcoRI FseI FspI FspAI
HpaI KpnI MfeI NdeI NheI NruI PacI PflMI
PmeI PmlI PsiI PsrI RsrII SbfI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XmaI ZraI