Restriction map for
pFN21AE3447
(Linear) (Six-base) MAPSORT of: KIEE3447.seq Check: 2030 from: 1 to: 3843
KIEE3447.orf
With 182 enzymes: SgfI *
July 3, 2012 17:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1012 1095 2190 3533 3843
Size: 1012 83 1095 1343 310
Fragments arranged by size:
1343 1095 1012 310 83
AccI GT'mk_AC
Cuts at: 0 1630 3547 3843
Size: 1630 1917 296
Fragments arranged by size:
1917 1630 296
Acc65I G'GTAC_C
Cuts at: 0 1511 3843
Size: 1511 2332
AclI AA'CG_TT
Cuts at: 0 545 3843
Size: 545 3298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1382 1559 1920 3013 3242 3843
Size: 1382 177 361 1093 229 601
Fragments arranged by size:
1382 1093 601 361 229 177
AflII C'TTAA_G
Cuts at: 0 1159 1528 3843
Size: 1159 369 2315
Fragments arranged by size:
2315 1159 369
AflIII A'CryG_T
Cuts at: 0 1652 1853 1896 3843
Size: 1652 201 43 1947
Fragments arranged by size:
1947 1652 201 43
AleI CACnn'nnGTG
Cuts at: 0 694 3667 3843
Size: 694 2973 176
Fragments arranged by size:
2973 694 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2647 3685 3843
Size: 2647 1038 158
Fragments arranged by size:
2647 1038 158
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 871 903 3843
Size: 871 32 2940
Fragments arranged by size:
2940 871 32
AlwNI CAG_nnn'CTG
Cuts at: 0 59 448 478 528 2002 2086 2685 3046
Size: 59 389 30 50 1474 84 599 361
Cuts at: 3046 3843
Size: 797
Fragments arranged by size:
1474 797 599 389 361 84 59 50
30
ApaI G_GGCC'C
Cuts at: 0 3698 3843
Size: 3698 145
ApaLI G'TGCA_C
Cuts at: 0 1957 3670 3843
Size: 1957 1713 173
Fragments arranged by size:
1957 1713 173
ApoI r'AATT_y
Cuts at: 0 402 1060 2477 3843
Size: 402 658 1417 1366
Fragments arranged by size:
1417 1366 658 402
AvaI C'yCGr_G
Cuts at: 0 133 486 3776 3843
Size: 133 353 3290 67
Fragments arranged by size:
3290 353 133 67
AvrII C'CTAG_G
Cuts at: 0 798 3843
Size: 798 3045
BamHI G'GATC_C
Cuts at: 0 17 3109 3843
Size: 17 3092 734
Fragments arranged by size:
3092 734 17
BanI G'GyrC_C
Cuts at: 0 36 1511 2268 2385 3154 3494 3843
Size: 36 1475 757 117 769 340 349
Fragments arranged by size:
1475 769 757 349 340 117 36
BanII G_rGCy'C
Cuts at: 0 296 1293 3312 3586 3698 3843
Size: 296 997 2019 274 112 145
Fragments arranged by size:
2019 997 296 274 145 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 3764 3843
Size: 3764 79
BciVI GTATCCnnnnn_n'
Cuts at: 0 3793 3843
Size: 3793 50
BclI T'GATC_A
Cuts at: 0 1407 3843
Size: 1407 2436
BglI GCCn_nnn'nGGC
Cuts at: 0 3721 3843
Size: 3721 122
BglII A'GATC_T
Cuts at: 0 1832 3843
Size: 1832 2011
BlpI GC'TnA_GC
Cuts at: 0 1361 2279 2678 3843
Size: 1361 918 399 1165
Fragments arranged by size:
1361 1165 918 399
Bme1580I G_kGCm'C
Cuts at: 0 1961 2390 3497 3674 3698 3843
Size: 1961 429 1107 177 24 145
Fragments arranged by size:
1961 1107 429 177 145 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1354 2972 3589 3843
Size: 1354 1618 617 254
Fragments arranged by size:
1618 1354 617 254
BmtI G_CTAG'C
Cuts at: 0 2584 3843
Size: 2584 1259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1573 1660 2108 2483 2610 2747 3023 3843
Size: 1573 87 448 375 127 137 276 820
Fragments arranged by size:
1573 820 448 375 276 137 127 87
Bpu10I CC'TnA_GC
Cuts at: 0 934 1616 3232 3843
Size: 934 682 1616 611
Fragments arranged by size:
1616 934 682 611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 1063 1114 2339 3612 3843
Size: 577 486 51 1225 1273 231
Fragments arranged by size:
1273 1225 577 486 231 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 3232 3843
Size: 3232 611
BsaAI yAC'GTr
Cuts at: 0 3551 3843
Size: 3551 292
BsaBI GATnn'nnATC
Cuts at: 0 2879 3843
Size: 2879 964
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 95 125 871 901 3843
Size: 95 30 746 30 2942
Fragments arranged by size:
2942 746 95 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 375 424 427 855 3694 3843
Size: 375 49 3 428 2839 149
Fragments arranged by size:
2839 428 375 149 49 3
BseYI C'CCAG_C
Cuts at: 0 302 360 441 2096 2908 3405 3697 3843
Size: 302 58 81 1655 812 497 292 146
Fragments arranged by size:
1655 812 497 302 292 146 81 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 640 2060 3186 3350 3560 3843
Size: 181 459 1420 1126 164 210 283
Fragments arranged by size:
1420 1126 459 283 210 181 164
BsiEI CG_ry'CG
Cuts at: 0 174 2415 2419 3843
Size: 174 2241 4 1424
Fragments arranged by size:
2241 1424 174 4
BsiHKAI G_wGCw'C
Cuts at: 0 847 1261 1293 1961 3093 3312 3674 3843
Size: 847 414 32 668 1132 219 362 169
Fragments arranged by size:
1132 847 668 414 362 219 169 32
BsmI GAATG_Cn'
Cuts at: 0 2217 2601 3843
Size: 2217 384 1242
Fragments arranged by size:
2217 1242 384
Bsp1286I G_dGCh'C
Cuts at: 0 296 847 1261 1293 1961 2390 3093 3312
Size: 296 551 414 32 668 429 703 219
Cuts at: 3312 3497 3586 3674 3698 3843
Size: 185 89 88 24 145
Fragments arranged by size:
703 668 551 429 414 296 219 185
145 89 88 32 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1012 1095 2190 3533 3843
Size: 1012 83 1095 1343 310
Fragments arranged by size:
1343 1095 1012 310 83
BsrDI GCAATG_nn'
Cuts at: 0 2552 3596 3843
Size: 2552 1044 247
Fragments arranged by size:
2552 1044 247
BsrFI r'CCGG_y
Cuts at: 0 2410 2414 2418 3843
Size: 2410 4 4 1425
Fragments arranged by size:
2410 1425 4 4
BsrGI T'GTAC_A
Cuts at: 0 2323 3843
Size: 2323 1520
BstAPI GCAn_nnn'nTGC
Cuts at: 0 59 2002 2275 2505 3843
Size: 59 1943 273 230 1338
Fragments arranged by size:
1943 1338 273 230 59
BstBI TT'CG_AA
Cuts at: 0 2740 3843
Size: 2740 1103
BstEII G'GTnAC_C
Cuts at: 0 1310 3843
Size: 1310 2533
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2608 3843
Size: 2608 1235
BstYI r'GATC_y
Cuts at: 0 17 755 1497 1832 2148 3109 3843
Size: 17 738 742 335 316 961 734
Fragments arranged by size:
961 742 738 734 335 316 17
BstZ17I GTA'TAC
Cuts at: 0 3548 3843
Size: 3548 295
Bsu36I CC'TnA_GG
Cuts at: 0 3222 3843
Size: 3222 621
BtgI C'CryG_G
Cuts at: 0 2561 3843
Size: 2561 1282
BtsI GCAGTG_nn'
Cuts at: 0 69 276 458 498 945 1069 2672 3843
Size: 69 207 182 40 447 124 1603 1171
Fragments arranged by size:
1603 1171 447 207 182 124 69 40
DraI TTT'AAA
Cuts at: 0 44 3843
Size: 44 3799
EaeI y'GGCC_r
Cuts at: 0 1109 2412 2416 2669 3843
Size: 1109 1303 4 253 1174
Fragments arranged by size:
1303 1174 1109 253 4
EagI C'GGCC_G
Cuts at: 0 2412 2416 3843
Size: 2412 4 1427
Fragments arranged by size:
2412 1427 4
EarI CTCTTCn'nnn_
Cuts at: 0 832 1671 2775 2951 3057 3843
Size: 832 839 1104 176 106 786
Fragments arranged by size:
1104 839 832 786 176 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 193 3843
Size: 193 3650
EcoICRI GAG'CTC
Cuts at: 0 1291 3310 3843
Size: 1291 2019 533
Fragments arranged by size:
2019 1291 533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1382 1559 1573 1660 1920 2108 2483 2610
Size: 1382 177 14 87 260 188 375 127
Cuts at: 2610 2747 3013 3023 3242 3843
Size: 137 266 10 219 601
Fragments arranged by size:
1382 601 375 266 260 219 188 177
137 127 87 14 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 3843
Size: 133 3710
EcoO109I rG'GnC_Cy
Cuts at: 0 88 429 3124 3678 3843
Size: 88 341 2695 554 165
Fragments arranged by size:
2695 554 341 165 88
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1902 3843
Size: 1902 1941
FseI GG_CCGG'CC
Cuts at: 0 2418 3843
Size: 2418 1425
FspI TGC'GCA
Cuts at: 0 2406 3843
Size: 2406 1437
HaeII r_GCGC'y
Cuts at: 0 2425 3843
Size: 2425 1418
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 516 548 1125 1157 1199 1231 1324 1356
Size: 516 32 577 32 42 32 93 32
Cuts at: 1356 1758 1790 1866 1898 2124 2156 3034 3066
Size: 402 32 76 32 226 32 878 32
Cuts at: 3066 3272 3304 3843
Size: 206 32 539
Fragments arranged by size:
878 577 539 516 402 226 206 93
76 42 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 603 3843
Size: 603 3240
KpnI G_GTAC'C
Cuts at: 0 1515 3843
Size: 1515 2328
MfeI C'AATT_G
Cuts at: 0 1712 2255 3843
Size: 1712 543 1588
Fragments arranged by size:
1712 1588 543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 2181 3403 3843
Size: 1004 1177 1222 440
Fragments arranged by size:
1222 1177 1004 440
MscI TGG'CCA
Cuts at: 0 1111 2671 3843
Size: 1111 1560 1172
Fragments arranged by size:
1560 1172 1111
MslI CAynn'nnrTG
Cuts at: 0 694 978 1856 2545 2606 2629 3279 3667
Size: 694 284 878 689 61 23 650 388
Cuts at: 3667 3843
Size: 176
Fragments arranged by size:
878 694 689 650 388 284 176 61
23
MspA1I CmG'CkG
Cuts at: 0 195 225 478 722 741 1447 2002 2275
Size: 195 30 253 244 19 706 555 273
Cuts at: 2275 2443 3807 3843
Size: 168 1364 36
Fragments arranged by size:
1364 706 555 273 253 244 195 168
36 30 19
NaeI GCC'GGC
Cuts at: 0 2412 2416 2420 3843
Size: 2412 4 4 1423
Fragments arranged by size:
2412 1423 4 4
NcoI C'CATG_G
Cuts at: 0 2561 3843
Size: 2561 1282
NgoMIV G'CCGG_C
Cuts at: 0 2410 2414 2418 3843
Size: 2410 4 4 1425
Fragments arranged by size:
2410 1425 4 4
NheI G'CTAG_C
Cuts at: 0 2580 3843
Size: 2580 1263
NspI r_CATG'y
Cuts at: 0 1656 1857 1900 3637 3843
Size: 1656 201 43 1737 206
Fragments arranged by size:
1737 1656 206 201 43
PciI A'CATG_T
Cuts at: 0 1652 1853 1896 3843
Size: 1652 201 43 1947
Fragments arranged by size:
1947 1652 201 43
PflMI CCAn_nnn'nTGG
Cuts at: 0 2086 3843
Size: 2086 1757
PfoI T'CCnGG_A
Cuts at: 0 2085 3843
Size: 2085 1758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 699 731 871 903 3843
Size: 699 32 140 32 2940
Fragments arranged by size:
2940 699 140 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3678 3843
Size: 3678 165
PsiI TTA'TAA
Cuts at: 0 2852 3843
Size: 2852 991
PspOMI G'GGCC_C
Cuts at: 0 3694 3843
Size: 3694 149
PspXI vC'TCGA_Gb
Cuts at: 0 133 3843
Size: 133 3710
PstI C_TGCA'G
Cuts at: 0 64 271 453 1998 2453 3642 3843
Size: 64 207 182 1545 455 1189 201
Fragments arranged by size:
1545 1189 455 207 201 182 64
PvuI CG_AT'CG
Cuts at: 0 174 3843
Size: 174 3669
PvuII CAG'CTG
Cuts at: 0 195 225 741 1447 2002 2275 3843
Size: 195 30 516 706 555 273 1568
Fragments arranged by size:
1568 706 555 516 273 195 30
SacI G_AGCT'C
Cuts at: 0 1293 3312 3843
Size: 1293 2019 531
Fragments arranged by size:
2019 1293 531
ScaI AGT'ACT
Cuts at: 0 164 3843
Size: 164 3679
SexAI A'CCwGG_T
Cuts at: 0 392 1480 3674 3843
Size: 392 1088 2194 169
Fragments arranged by size:
2194 1088 392 169
SfcI C'TryA_G
Cuts at: 0 60 267 449 1284 1631 1994 2287 2449
Size: 60 207 182 835 347 363 293 162
Cuts at: 2449 3206 3638 3843
Size: 757 432 205
Fragments arranged by size:
835 757 432 363 347 293 207 205
182 162 60
SmlI C'TyrA_G
Cuts at: 0 133 556 1078 1129 1159 1528 2354 3627
Size: 133 423 522 51 30 369 826 1273
Cuts at: 3627 3843
Size: 216
Fragments arranged by size:
1273 826 522 423 369 216 133 51
30
SnaBI TAC'GTA
Cuts at: 0 3551 3843
Size: 3551 292
SphI G_CATG'C
Cuts at: 0 3637 3843
Size: 3637 206
SspI AAT'ATT
Cuts at: 0 337 3843
Size: 337 3506
StuI AGG'CCT
Cuts at: 0 3220 3843
Size: 3220 623
StyI C'CwwG_G
Cuts at: 0 798 848 2561 2858 2998 3843
Size: 798 50 1713 297 140 845
Fragments arranged by size:
1713 845 798 297 140 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 661 1688 2143 3512 3769 3843
Size: 661 1027 455 1369 257 74
Fragments arranged by size:
1369 1027 661 455 257 74
TatI w'GTAC_w
Cuts at: 0 162 1082 1586 2323 2445 2618 3843
Size: 162 920 504 737 122 173 1225
Fragments arranged by size:
1225 920 737 504 173 162 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 95 127 2216 2248 3843
Size: 95 32 2089 32 1595
Fragments arranged by size:
2089 1595 95 32 32
XbaI T'CTAG_A
Cuts at: 0 2784 3843
Size: 2784 1059
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 855 3763 3843
Size: 855 2908 80
Fragments arranged by size:
2908 855 80
XhoI C'TCGA_G
Cuts at: 0 133 3843
Size: 133 3710
XmnI GAAnn'nnTTC
Cuts at: 0 1301 1454 3843
Size: 1301 153 2389
Fragments arranged by size:
2389 1301 153
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII
BamHI BanI BanII BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI FspI HaeII Hin4I HincII KpnI MfeI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
NspI PciI PflMI PfoI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII SacI ScaI SexAI SfcI
SmlI SnaBI SphI SspI StuI StyI TaqII TatI
TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AscI AseI AsiSI BaeI
BbeI BbvCI BcgI BfrBI BmgBI BplI BsaHI BsaWI
BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI BtgZI
ClaI CspCI DraIII DrdI EcoRI EcoRV FspAI HindIII
HpaI KasI MluI NarI NdeI NotI NruI NsiI
PacI PasI PmeI PmlI PshAI PsrI RsrII SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SpeI SrfI SwaI Tth111I XmaI ZraI