Restriction map for
pFN21AE3437
(Linear) (Six-base) MAPSORT of: KIEE3437.seq Check: 9173 from: 1 to: 2889
KIEE3437.orf
With 182 enzymes: SgfI *
July 3, 2012 17:51 ..
AatII G_ACGT'C
Cuts at: 0 2000 2889
Size: 2000 889
AccI GT'mk_AC
Cuts at: 0 1775 2889
Size: 1775 1114
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1620 2222 2889
Size: 1620 602 667
Fragments arranged by size:
1620 667 602
AflIII A'CryG_T
Cuts at: 0 881 947 1137 2889
Size: 881 66 190 1752
Fragments arranged by size:
1752 881 190 66
AleI CACnn'nnGTG
Cuts at: 0 1396 2368 2530 2889
Size: 1396 972 162 359
Fragments arranged by size:
1396 972 359 162
AlwNI CAG_nnn'CTG
Cuts at: 0 157 481 1209 2889
Size: 157 324 728 1680
Fragments arranged by size:
1680 728 324 157
ApaI G_GGCC'C
Cuts at: 0 2009 2057 2889
Size: 2009 48 832
Fragments arranged by size:
2009 832 48
AvaI C'yCGr_G
Cuts at: 0 683 1865 2510 2889
Size: 683 1182 645 379
Fragments arranged by size:
1182 683 645 379
BamHI G'GATC_C
Cuts at: 0 1509 2889
Size: 1509 1380
BanI G'GyrC_C
Cuts at: 0 245 428 2078 2830 2889
Size: 245 183 1650 752 59
Fragments arranged by size:
1650 752 245 183 59
BanII G_rGCy'C
Cuts at: 0 32 707 1037 1750 2009 2057 2360 2889
Size: 32 675 330 713 259 48 303 529
Fragments arranged by size:
713 675 529 330 303 259 48 32
BbeI G_GCGC'C
Cuts at: 0 249 432 2834 2889
Size: 249 183 2402 55
Fragments arranged by size:
2402 249 183 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 73 719 1485 1626 2889
Size: 73 646 766 141 1263
Fragments arranged by size:
1263 766 646 141 73
BbvCI CC'TCA_GC
Cuts at: 0 979 2282 2889
Size: 979 1303 607
Fragments arranged by size:
1303 979 607
BclI T'GATC_A
Cuts at: 0 812 2889
Size: 812 2077
BglI GCCn_nnn'nGGC
Cuts at: 0 2127 2889
Size: 2127 762
BglII A'GATC_T
Cuts at: 0 1562 2889
Size: 1562 1327
Bme1580I G_kGCm'C
Cuts at: 0 1405 1865 2009 2057 2083 2889
Size: 1405 460 144 48 26 806
Fragments arranged by size:
1405 806 460 144 48 26
BmgBI CAC'GTC
Cuts at: 0 2793 2889
Size: 2793 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 580 812 1063 2024 2247 2889
Size: 580 232 251 961 223 642
Fragments arranged by size:
961 642 580 251 232 223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1048 2084 2401 2889
Size: 354 694 1036 317 488
Fragments arranged by size:
1036 694 488 354 317
Bpu10I CC'TnA_GC
Cuts at: 0 979 2036 2282 2889
Size: 979 1057 246 607
Fragments arranged by size:
1057 979 607 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 817 1734 2889
Size: 418 399 917 1155
Fragments arranged by size:
1155 917 418 399
BsaI GGTCTCn'nnnn_
Cuts at: 0 817 2024 2368 2889
Size: 817 1207 344 521
Fragments arranged by size:
1207 817 521 344
BsaAI yAC'GTr
Cuts at: 0 348 950 1926 2292 2316 2889
Size: 348 602 976 366 24 573
Fragments arranged by size:
976 602 573 366 348 24
BsaBI GATnn'nnATC
Cuts at: 0 2470 2889
Size: 2470 419
BsaHI Gr'CG_yC
Cuts at: 0 78 246 429 1997 2802 2831 2889
Size: 78 168 183 1568 805 29 58
Fragments arranged by size:
1568 805 183 168 78 58 29
BsaWI w'CCGG_w
Cuts at: 0 57 1299 2598 2889
Size: 57 1242 1299 291
Fragments arranged by size:
1299 1242 291 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 352 382 1108 1138 1198 1228 1798 1828
Size: 352 30 726 30 60 30 570 30
Cuts at: 1828 1832 1862 1876 1906 2710 2740 2889
Size: 4 30 14 30 804 30 149
Fragments arranged by size:
804 726 570 352 149 60 30 30
30 30 30 30 30 14 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 1045 1684 1996 2889
Size: 232 813 639 312 893
Fragments arranged by size:
893 813 639 312 232
BseYI C'CCAG_C
Cuts at: 0 474 850 1291 2050 2056 2216 2835 2863
Size: 474 376 441 759 6 160 619 28
Cuts at: 2863 2889
Size: 26
Fragments arranged by size:
759 619 474 441 376 160 28 26
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 2889
Size: 370 2519
BsiEI CG_ry'CG
Cuts at: 0 140 1300 2187 2819 2889
Size: 140 1160 887 632 70
Fragments arranged by size:
1160 887 632 140 70
BsiHKAI G_wGCw'C
Cuts at: 0 1037 2360 2889
Size: 1037 1323 529
Fragments arranged by size:
1323 1037 529
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2730 2889
Size: 2730 159
Bsp1286I G_dGCh'C
Cuts at: 0 32 707 1037 1405 1750 1865 2009 2057
Size: 32 675 330 368 345 115 144 48
Cuts at: 2057 2083 2360 2889
Size: 26 277 529
Fragments arranged by size:
675 529 368 345 330 277 144 115
48 32 26
BspEI T'CCGG_A
Cuts at: 0 57 2598 2889
Size: 57 2541 291
Fragments arranged by size:
2541 291 57
BspHI T'CATG_A
Cuts at: 0 869 956 2889
Size: 869 87 1933
Fragments arranged by size:
1933 869 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1787 2889
Size: 1787 1102
BsrBI CCG'CTC
Cuts at: 0 690 1124 2889
Size: 690 434 1765
Fragments arranged by size:
1765 690 434
BsrDI GCAATG_nn'
Cuts at: 0 990 1422 2225 2889
Size: 990 432 803 664
Fragments arranged by size:
990 803 664 432
BsrFI r'CCGG_y
Cuts at: 0 292 1535 1910 2818 2889
Size: 292 1243 375 908 71
Fragments arranged by size:
1243 908 375 292 71
BsrGI T'GTAC_A
Cuts at: 0 2606 2889
Size: 2606 283
BssSI C'ACGA_G
Cuts at: 0 214 1540 2530 2889
Size: 214 1326 990 359
Fragments arranged by size:
1326 990 359 214
BstAPI GCAn_nnn'nTGC
Cuts at: 0 950 2889
Size: 950 1939
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2442 2889
Size: 2442 447
BstYI r'GATC_y
Cuts at: 0 1509 1562 1569 2889
Size: 1509 53 7 1320
Fragments arranged by size:
1509 1320 53 7
BtgI C'CryG_G
Cuts at: 0 1374 1505 2889
Size: 1374 131 1384
Fragments arranged by size:
1384 1374 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 142 1298 2607 2889
Size: 142 1156 1309 282
Fragments arranged by size:
1309 1156 282 142
BtsI GCAGTG_nn'
Cuts at: 0 283 340 979 1459 2889
Size: 283 57 639 480 1430
Fragments arranged by size:
1430 639 480 283 57
DrdI GACnn_nn'nnGTC
Cuts at: 0 2722 2889
Size: 2722 167
EaeI y'GGCC_r
Cuts at: 0 522 1113 1158 2694 2816 2889
Size: 522 591 45 1536 122 73
Fragments arranged by size:
1536 591 522 122 73 45
EagI C'GGCC_G
Cuts at: 0 2816 2889
Size: 2816 73
EarI CTCTTCn'nnn_
Cuts at: 0 108 884 1387 2365 2779 2889
Size: 108 776 503 978 414 110
Fragments arranged by size:
978 776 503 414 110 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1359 2889
Size: 1359 1530
EcoICRI GAG'CTC
Cuts at: 0 1035 2358 2889
Size: 1035 1323 531
Fragments arranged by size:
1323 1035 531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1048 1620 2084 2222 2401 2889
Size: 354 694 572 464 138 179 488
Fragments arranged by size:
694 572 488 464 354 179 138
EcoNI CCTnn'n_nnAGG
Cuts at: 0 436 2383 2889
Size: 436 1947 506
Fragments arranged by size:
1947 506 436
EcoO109I rG'GnC_Cy
Cuts at: 0 1242 1887 2006 2550 2889
Size: 1242 645 119 544 339
Fragments arranged by size:
1242 645 544 339 119
EcoRV GAT'ATC
Cuts at: 0 1704 2889
Size: 1704 1185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1500 2889
Size: 1500 1389
FspI TGC'GCA
Cuts at: 0 676 2889
Size: 676 2213
HaeII r_GCGC'y
Cuts at: 0 249 432 2834 2889
Size: 249 183 2402 55
Fragments arranged by size:
2402 249 183 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 1198 1230 1554 1586 1745 1777
Size: 352 32 814 32 324 32 159 32
Cuts at: 1777 1832 1864 2889
Size: 55 32 1025
Fragments arranged by size:
1025 814 352 324 159 55 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 245 428 2830 2889
Size: 245 183 2402 59
Fragments arranged by size:
2402 245 183 59
MfeI C'AATT_G
Cuts at: 0 2097 2889
Size: 2097 792
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 2657 2889
Size: 866 1791 232
Fragments arranged by size:
1791 866 232
MscI TGG'CCA
Cuts at: 0 524 1115 1160 2889
Size: 524 591 45 1729
Fragments arranged by size:
1729 591 524 45
MslI CAynn'nnrTG
Cuts at: 0 1396 2115 2368 2530 2889
Size: 1396 719 253 162 359
Fragments arranged by size:
1396 719 359 253 162
MspA1I CmG'CkG
Cuts at: 0 98 154 184 481 503 861 2145 2427
Size: 98 56 30 297 22 358 1284 282
Cuts at: 2427 2693 2889
Size: 266 196
Fragments arranged by size:
1284 358 297 282 266 196 98 56
30 22
NaeI GCC'GGC
Cuts at: 0 294 2820 2889
Size: 294 2526 69
Fragments arranged by size:
2526 294 69
NarI GG'CG_CC
Cuts at: 0 246 429 2831 2889
Size: 246 183 2402 58
Fragments arranged by size:
2402 246 183 58
NcoI C'CATG_G
Cuts at: 0 1505 2889
Size: 1505 1384
NgoMIV G'CCGG_C
Cuts at: 0 292 2818 2889
Size: 292 2526 71
Fragments arranged by size:
2526 292 71
NspI r_CATG'y
Cuts at: 0 868 885 1141 2652 2889
Size: 868 17 256 1511 237
Fragments arranged by size:
1511 868 256 237 17
PasI CC'CwG_GG
Cuts at: 0 2002 2889
Size: 2002 887
PciI A'CATG_T
Cuts at: 0 881 1137 2889
Size: 881 256 1752
Fragments arranged by size:
1752 881 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 1179 2793 2889
Size: 1179 1614 96
Fragments arranged by size:
1614 1179 96
PfoI T'CCnGG_A
Cuts at: 0 44 1223 2090 2546 2889
Size: 44 1179 867 456 343
Fragments arranged by size:
1179 867 456 343 44
PmlI CAC'GTG
Cuts at: 0 950 2889
Size: 950 1939
PpuMI rG'GwC_Cy
Cuts at: 0 1242 2550 2889
Size: 1242 1308 339
Fragments arranged by size:
1308 1242 339
PspOMI G'GGCC_C
Cuts at: 0 2005 2053 2889
Size: 2005 48 836
Fragments arranged by size:
2005 836 48
PstI C_TGCA'G
Cuts at: 0 974 2159 2345 2854 2889
Size: 974 1185 186 509 35
Fragments arranged by size:
1185 974 509 186 35
PvuII CAG'CTG
Cuts at: 0 98 154 481 503 861 2145 2427 2889
Size: 98 56 327 22 358 1284 282 462
Fragments arranged by size:
1284 462 358 327 282 98 56 22
SacI G_AGCT'C
Cuts at: 0 1037 2360 2889
Size: 1037 1323 529
Fragments arranged by size:
1323 1037 529
SapI GCTCTTCn'nnn_
Cuts at: 0 2365 2889
Size: 2365 524
ScaI AGT'ACT
Cuts at: 0 1006 2889
Size: 1006 1883
SexAI A'CCwGG_T
Cuts at: 0 494 1091 2889
Size: 494 597 1798
Fragments arranged by size:
1798 597 494
SfcI C'TryA_G
Cuts at: 0 108 970 2155 2341 2850 2889
Size: 108 862 1185 186 509 39
Fragments arranged by size:
1185 862 509 186 108 39
SfoI GGC'GCC
Cuts at: 0 247 430 2832 2889
Size: 247 183 2402 57
Fragments arranged by size:
2402 247 183 57
SmaI CCC'GGG
Cuts at: 0 1867 2889
Size: 1867 1022
SmlI C'TyrA_G
Cuts at: 0 433 796 1749 2889
Size: 433 363 953 1140
Fragments arranged by size:
1140 953 433 363
SphI G_CATG'C
Cuts at: 0 2652 2889
Size: 2652 237
StuI AGG'CCT
Cuts at: 0 230 1081 2889
Size: 230 851 1808
Fragments arranged by size:
1808 851 230
StyI C'CwwG_G
Cuts at: 0 1505 2103 2125 2756 2889
Size: 1505 598 22 631 133
Fragments arranged by size:
1505 631 598 133 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 546 2877 2889
Size: 546 2331 12
Fragments arranged by size:
2331 546 12
TatI w'GTAC_w
Cuts at: 0 1004 2606 2889
Size: 1004 1602 283
Fragments arranged by size:
1602 1004 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 226 258 1108 1140 2889
Size: 226 32 850 32 1749
Fragments arranged by size:
1749 850 226 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2305 2889
Size: 2305 584
XbaI T'CTAG_A
Cuts at: 0 513 2889
Size: 513 2376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 482 2889
Size: 482 2407
XmaI C'CCGG_G
Cuts at: 0 1865 2889
Size: 1865 1024
ZraI GAC'GTC
Cuts at: 0 1998 2889
Size: 1998 891
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BclI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SapI ScaI SexAI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BfrBI BlpI BmtI BplI BsiWI BsmI
BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DraIII EcoRI FseI FspAI HincII HindIII HpaI KpnI
MluI NdeI NheI NotI NruI NsiI PacI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XhoI XmnI