Restriction map for
pFN21AE3434
(Linear) (Six-base) MAPSORT of: KIEE3434.seq Check: 3587 from: 1 to: 2658
KIEE3434.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1183 2658
Size: 1183 1475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 539 1234 1687 2658
Size: 97 442 695 453 971
Fragments arranged by size:
971 695 453 442 97
AflIII A'CryG_T
Cuts at: 0 464 676 1634 2658
Size: 464 212 958 1024
Fragments arranged by size:
1024 958 464 212
AleI CACnn'nnGTG
Cuts at: 0 1243 2658
Size: 1243 1415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2576 2658
Size: 2576 82
AlwNI CAG_nnn'CTG
Cuts at: 0 96 552 2582 2626 2658
Size: 96 456 2030 44 32
Fragments arranged by size:
2030 456 96 44 32
ApaI G_GGCC'C
Cuts at: 0 56 2658
Size: 56 2602
ApaLI G'TGCA_C
Cuts at: 0 1759 1948 2181 2363 2658
Size: 1759 189 233 182 295
Fragments arranged by size:
1759 295 233 189 182
ApoI r'AATT_y
Cuts at: 0 1219 2401 2658
Size: 1219 1182 257
Fragments arranged by size:
1219 1182 257
AvaI C'yCGr_G
Cuts at: 0 49 56 692 1504 2071 2393 2499 2508
Size: 49 7 636 812 567 322 106 9
Cuts at: 2508 2658
Size: 150
Fragments arranged by size:
812 636 567 322 150 106 49 9
7
BanI G'GyrC_C
Cuts at: 0 280 2658
Size: 280 2378
BanII G_rGCy'C
Cuts at: 0 56 385 809 1091 2059 2658
Size: 56 329 424 282 968 599
Fragments arranged by size:
968 599 424 329 282 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 527 1078 1675 2658
Size: 527 551 597 983
Fragments arranged by size:
983 597 551 527
BbvCI CC'TCA_GC
Cuts at: 0 1573 1774 2658
Size: 1573 201 884
Fragments arranged by size:
1573 884 201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 303 337 733 767 2658
Size: 303 34 396 34 1891
Fragments arranged by size:
1891 396 303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 136 2658
Size: 136 2522
BfrBI ATG'CAT
Cuts at: 0 1026 2658
Size: 1026 1632
BglI GCCn_nnn'nGGC
Cuts at: 0 186 409 2194 2257 2510 2569 2658
Size: 186 223 1785 63 253 59 89
Fragments arranged by size:
1785 253 223 186 89 63 59
BglII A'GATC_T
Cuts at: 0 2000 2658
Size: 2000 658
Bme1580I G_kGCm'C
Cuts at: 0 56 605 1763 1952 2185 2367 2658
Size: 56 549 1158 189 233 182 291
Fragments arranged by size:
1158 549 291 233 189 182 56
BmgBI CAC'GTC
Cuts at: 0 1287 1320 2658
Size: 1287 33 1338
Fragments arranged by size:
1338 1287 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 605 2658
Size: 605 2053
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 53 789 2078 2658
Size: 53 736 1289 580
Fragments arranged by size:
1289 736 580 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 745 754 1126 1178 1285 1402 1816
Size: 724 21 9 372 52 107 117 414
Cuts at: 1816 2158 2329 2341 2658
Size: 342 171 12 317
Fragments arranged by size:
724 414 372 342 317 171 117 107
52 21 12 9
Bpu10I CC'TnA_GC
Cuts at: 0 545 1573 1701 1774 2658
Size: 545 1028 128 73 884
Fragments arranged by size:
1028 884 545 128 73
BsaI GGTCTCn'nnnn_
Cuts at: 0 730 852 1443 1675 1957 2642 2658
Size: 730 122 591 232 282 685 16
Fragments arranged by size:
730 685 591 282 232 122 16
BsaAI yAC'GTr
Cuts at: 0 1338 2658
Size: 1338 1320
BsaBI GATnn'nnATC
Cuts at: 0 1424 1609 2658
Size: 1424 185 1049
Fragments arranged by size:
1424 1049 185
BsaWI w'CCGG_w
Cuts at: 0 563 835 1748 2376 2658
Size: 563 272 913 628 282
Fragments arranged by size:
913 628 563 282 272
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 333 363 558 588 1891 1921 2314 2344
Size: 333 30 195 30 1303 30 393 30
Cuts at: 2344 2658
Size: 314
Fragments arranged by size:
1303 393 333 314 195 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 721 2089 2092 2155 2164 2658
Size: 721 1368 3 63 9 494
Fragments arranged by size:
1368 721 494 63 9 3
BseYI C'CCAG_C
Cuts at: 0 630 956 2619 2658
Size: 630 326 1663 39
Fragments arranged by size:
1663 630 326 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1291 1340 2658
Size: 382 909 49 1318
Fragments arranged by size:
1318 909 382 49
BsiEI CG_ry'CG
Cuts at: 0 14 225 1734 2531 2658
Size: 14 211 1509 797 127
Fragments arranged by size:
1509 797 211 127 14
BsiHKAI G_wGCw'C
Cuts at: 0 809 1091 1505 1763 1952 2185 2367 2555
Size: 809 282 414 258 189 233 182 188
Cuts at: 2555 2658
Size: 103
Fragments arranged by size:
809 414 282 258 233 189 188 182
103
Bsp1286I G_dGCh'C
Cuts at: 0 56 385 605 809 1091 1505 1763 1952
Size: 56 329 220 204 282 414 258 189
Cuts at: 1952 2059 2185 2367 2555 2658
Size: 107 126 182 188 103
Fragments arranged by size:
414 329 282 258 220 204 189 188
182 126 107 103 56
BspEI T'CCGG_A
Cuts at: 0 563 835 1748 2376 2658
Size: 563 272 913 628 282
Fragments arranged by size:
913 628 563 282 272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 821 1183 1217 2658
Size: 821 362 34 1441
Fragments arranged by size:
1441 821 362 34
BsrBI CCG'CTC
Cuts at: 0 168 174 446 1166 1203 2658
Size: 168 6 272 720 37 1455
Fragments arranged by size:
1455 720 272 168 37 6
BsrFI r'CCGG_y
Cuts at: 0 185 224 283 2658
Size: 185 39 59 2375
Fragments arranged by size:
2375 185 59 39
BsrGI T'GTAC_A
Cuts at: 0 1184 1484 2658
Size: 1184 300 1174
Fragments arranged by size:
1184 1174 300
BssHII G'CGCG_C
Cuts at: 0 159 198 2658
Size: 159 39 2460
Fragments arranged by size:
2460 159 39
BssSI C'ACGA_G
Cuts at: 0 391 2658
Size: 391 2267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1363 2203 2582 2658
Size: 1363 840 379 76
Fragments arranged by size:
1363 840 379 76
BstEII G'GTnAC_C
Cuts at: 0 498 1849 2658
Size: 498 1351 809
Fragments arranged by size:
1351 809 498
BstXI CCAn_nnnn'nTGG
Cuts at: 0 277 1794 2658
Size: 277 1517 864
Fragments arranged by size:
1517 864 277
BstYI r'GATC_y
Cuts at: 0 896 2000 2658
Size: 896 1104 658
Fragments arranged by size:
1104 896 658
BtgI C'CryG_G
Cuts at: 0 209 270 1523 1628 1673 1910 2522 2658
Size: 209 61 1253 105 45 237 612 136
Fragments arranged by size:
1253 612 237 209 136 105 61 45
BtsI GCAGTG_nn'
Cuts at: 0 43 1373 1901 2333 2367 2583 2658
Size: 43 1330 528 432 34 216 75
Fragments arranged by size:
1330 528 432 216 75 43 34
DraIII CAC_nnn'GTG
Cuts at: 0 1422 2658
Size: 1422 1236
DrdI GACnn_nn'nnGTC
Cuts at: 0 249 2658
Size: 249 2409
EaeI y'GGCC_r
Cuts at: 0 354 410 554 1346 1520 1625 1829 2528
Size: 354 56 144 792 174 105 204 699
Cuts at: 2528 2658
Size: 130
Fragments arranged by size:
792 699 354 204 174 144 130 105
56
EagI C'GGCC_G
Cuts at: 0 2528 2658
Size: 2528 130
EarI CTCTTCn'nnn_
Cuts at: 0 1079 1405 2141 2450 2658
Size: 1079 326 736 309 208
Fragments arranged by size:
1079 736 326 309 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 317 1987 2054 2658
Size: 317 1670 67 604
Fragments arranged by size:
1670 604 317 67
EcoICRI GAG'CTC
Cuts at: 0 807 1089 2658
Size: 807 282 1569
Fragments arranged by size:
1569 807 282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 539 724 745 754 1126 1178 1234
Size: 97 442 185 21 9 372 52 56
Cuts at: 1234 1285 1402 1687 1816 2158 2329 2341 2658
Size: 51 117 285 129 342 171 12 317
Fragments arranged by size:
442 372 342 317 285 185 171 129
117 97 56 52 51 21 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 24 2658
Size: 24 2634
EcoO109I rG'GnC_Cy
Cuts at: 0 53 1125 1206 1707 1743 2474 2658
Size: 53 1072 81 501 36 731 184
Fragments arranged by size:
1072 731 501 184 81 53 36
EcoRI G'AATT_C
Cuts at: 0 2401 2658
Size: 2401 257
FspI TGC'GCA
Cuts at: 0 238 2207 2586 2658
Size: 238 1969 379 72
Fragments arranged by size:
1969 379 238 72
HaeII r_GCGC'y
Cuts at: 0 321 426 940 1480 1584 2045 2658
Size: 321 105 514 540 104 461 613
Fragments arranged by size:
613 540 514 461 321 105 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 333 365 886 918 1711 1743 2065 2097
Size: 333 32 521 32 793 32 322 32
Cuts at: 2097 2371 2374 2403 2406 2593 2625 2658
Size: 274 3 29 3 187 32 33
Fragments arranged by size:
793 521 333 322 274 187 33 32
32 32 32 32 29 3 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1127 1466 1754 2261 2658
Size: 170 957 339 288 507 397
Fragments arranged by size:
957 507 397 339 288 170
MscI TGG'CCA
Cuts at: 0 556 1348 1627 2658
Size: 556 792 279 1031
Fragments arranged by size:
1031 792 556 279
MslI CAynn'nnrTG
Cuts at: 0 1186 1231 1243 1615 1633 1988 2658
Size: 1186 45 12 372 18 355 670
Fragments arranged by size:
1186 670 372 355 45 18 12
MspA1I CmG'CkG
Cuts at: 0 290 630 732 1315 1587 1794 2658
Size: 290 340 102 583 272 207 864
Fragments arranged by size:
864 583 340 290 272 207 102
NcoI C'CATG_G
Cuts at: 0 209 270 1673 2658
Size: 209 61 1403 985
Fragments arranged by size:
1403 985 209 61
NsiI A_TGCA'T
Cuts at: 0 1028 2658
Size: 1028 1630
NspI r_CATG'y
Cuts at: 0 252 468 1257 1638 2497 2658
Size: 252 216 789 381 859 161
Fragments arranged by size:
859 789 381 252 216 161
PasI CC'CwG_GG
Cuts at: 0 1432 2658
Size: 1432 1226
PciI A'CATG_T
Cuts at: 0 464 1634 2658
Size: 464 1170 1024
Fragments arranged by size:
1170 1024 464
PflMI CCAn_nnn'nTGG
Cuts at: 0 352 1104 1422 2252 2626 2658
Size: 352 752 318 830 374 32
Fragments arranged by size:
830 752 374 352 318 32
PfoI T'CCnGG_A
Cuts at: 0 484 872 1196 2658
Size: 484 388 324 1462
Fragments arranged by size:
1462 484 388 324
PpuMI rG'GwC_Cy
Cuts at: 0 1206 1707 1743 2474 2658
Size: 1206 501 36 731 184
Fragments arranged by size:
1206 731 501 184 36
PshAI GACnn'nnGTC
Cuts at: 0 719 2658
Size: 719 1939
PspOMI G'GGCC_C
Cuts at: 0 52 2658
Size: 52 2606
PstI C_TGCA'G
Cuts at: 0 1328 1544 2658
Size: 1328 216 1114
Fragments arranged by size:
1328 1114 216
PvuII CAG'CTG
Cuts at: 0 732 1315 2658
Size: 732 583 1343
Fragments arranged by size:
1343 732 583
SacI G_AGCT'C
Cuts at: 0 809 1091 2658
Size: 809 282 1567
Fragments arranged by size:
1567 809 282
SapI GCTCTTCn'nnn_
Cuts at: 0 1079 2450 2658
Size: 1079 1371 208
Fragments arranged by size:
1371 1079 208
SexAI A'CCwGG_T
Cuts at: 0 842 2658
Size: 842 1816
SfcI C'TryA_G
Cuts at: 0 1324 1540 2037 2658
Size: 1324 216 497 621
Fragments arranged by size:
1324 621 497 216
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2510 2658
Size: 2510 148
SmaI CCC'GGG
Cuts at: 0 51 58 694 2395 2501 2510 2658
Size: 51 7 636 1701 106 9 148
Fragments arranged by size:
1701 636 148 106 51 9 7
SmlI C'TyrA_G
Cuts at: 0 1504 2658
Size: 1504 1154
SphI G_CATG'C
Cuts at: 0 2497 2658
Size: 2497 161
SrfI GCCC'GGGC
Cuts at: 0 51 2658
Size: 51 2607
StyI C'CwwG_G
Cuts at: 0 209 270 1673 2658
Size: 209 61 1403 985
Fragments arranged by size:
1403 985 209 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 486 921 1090 1348 1868 1967 2000 2114
Size: 486 435 169 258 520 99 33 114
Cuts at: 2114 2658
Size: 544
Fragments arranged by size:
544 520 486 435 258 169 114 99
33
TatI w'GTAC_w
Cuts at: 0 854 1184 1484 2658
Size: 854 330 300 1174
Fragments arranged by size:
1174 854 330 300
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1891 1923 2658
Size: 1891 32 735
Fragments arranged by size:
1891 735 32
Tth111I GACn'n_nGTC
Cuts at: 0 480 1978 2478 2658
Size: 480 1498 500 180
Fragments arranged by size:
1498 500 480 180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 870 1419 1470 1791 2658
Size: 870 549 51 321 867
Fragments arranged by size:
870 867 549 321 51
XhoI C'TCGA_G
Cuts at: 0 1504 2658
Size: 1504 1154
XmaI C'CCGG_G
Cuts at: 0 49 56 692 2393 2499 2508 2658
Size: 49 7 636 1701 106 9 150
Fragments arranged by size:
1701 636 150 106 49 9 7
XmnI GAAnn'nnTTC
Cuts at: 0 1219 2658
Size: 1219 1439
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbsI BbvCI BcgI BciVI
BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I MmeI
MscI MslI MspA1I NcoI NsiI NspI PasI PciI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SapI SexAI SfcI SfiI SmaI SmlI SphI SrfI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BclI BlpI BmtI BpuEI BsaHI BsiWI BsmI BsmBI
BspHI BsrDI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfoI SgfI SgrAI SnaBI SpeI SspI StuI
SwaI XbaI ZraI