Restriction map for
pFN21AE3428
(Linear) (Six-base) MAPSORT of: KIEE3428.seq Check: 3209 from: 1 to: 1806
KIEE3428.orf
With 182 enzymes: SgfI *
March 22, 2012 16:33 ..
AccI GT'mk_AC
Cuts at: 0 306 1806
Size: 306 1500
Acc65I G'GTAC_C
Cuts at: 0 1656 1806
Size: 1656 150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 749 1313 1529 1726 1806
Size: 598 151 564 216 197 80
Fragments arranged by size:
598 564 216 197 151 80
AhdI GACnn_n'nnGTC
Cuts at: 0 861 1806
Size: 861 945
AleI CACnn'nnGTG
Cuts at: 0 1418 1806
Size: 1418 388
AlwNI CAG_nnn'CTG
Cuts at: 0 772 1744 1774 1806
Size: 772 972 30 32
Fragments arranged by size:
972 772 32 30
ApoI r'AATT_y
Cuts at: 0 762 1120 1238 1646 1806
Size: 762 358 118 408 160
Fragments arranged by size:
762 408 358 160 118
AseI AT'TA_AT
Cuts at: 0 478 1806
Size: 478 1328
AvaI C'yCGr_G
Cuts at: 0 980 1806
Size: 980 826
BanI G'GyrC_C
Cuts at: 0 128 1656 1806
Size: 128 1528 150
Fragments arranged by size:
1528 150 128
BanII G_rGCy'C
Cuts at: 0 79 1065 1806
Size: 79 986 741
Fragments arranged by size:
986 741 79
BbeI G_GCGC'C
Cuts at: 0 132 1806
Size: 132 1674
BbsI GAAGACnn'nnnn_
Cuts at: 0 224 1714 1806
Size: 224 1490 92
Fragments arranged by size:
1490 224 92
BbvCI CC'TCA_GC
Cuts at: 0 1244 1806
Size: 1244 562
BciVI GTATCCnnnnn_n'
Cuts at: 0 595 1806
Size: 595 1211
BclI T'GATC_A
Cuts at: 0 1575 1806
Size: 1575 231
BglI GCCn_nnn'nGGC
Cuts at: 0 1054 1806
Size: 1054 752
BglII A'GATC_T
Cuts at: 0 429 696 1693 1806
Size: 429 267 997 113
Fragments arranged by size:
997 429 267 113
BmrI ACTGGGnnnn_n'
Cuts at: 0 1754 1806
Size: 1754 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1806
Size: 887 919
Bpu10I CC'TnA_GC
Cuts at: 0 470 1244 1806
Size: 470 774 562
Fragments arranged by size:
774 562 470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 1049 1401 1806
Size: 521 528 352 405
Fragments arranged by size:
528 521 405 352
BsaI GGTCTCn'nnnn_
Cuts at: 0 194 1806
Size: 194 1612
BsaHI Gr'CG_yC
Cuts at: 0 129 1806
Size: 129 1677
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 1703 1806
Size: 358 1345 103
Fragments arranged by size:
1345 358 103
BseYI C'CCAG_C
Cuts at: 0 772 1806
Size: 772 1034
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1049 1806
Size: 1049 757
BsiHKAI G_wGCw'C
Cuts at: 0 873 1065 1806
Size: 873 192 741
Fragments arranged by size:
873 741 192
BsmI GAATG_Cn'
Cuts at: 0 391 662 1806
Size: 391 271 1144
Fragments arranged by size:
1144 391 271
Bsp1286I G_dGCh'C
Cuts at: 0 79 873 1065 1806
Size: 79 794 192 741
Fragments arranged by size:
794 741 192 79
BspHI T'CATG_A
Cuts at: 0 1578 1806
Size: 1578 228
BsrBI CCG'CTC
Cuts at: 0 64 1616 1806
Size: 64 1552 190
Fragments arranged by size:
1552 190 64
BsrDI GCAATG_nn'
Cuts at: 0 1610 1806
Size: 1610 196
BsrGI T'GTAC_A
Cuts at: 0 1227 1806
Size: 1227 579
BstBI TT'CG_AA
Cuts at: 0 420 1806
Size: 420 1386
BstYI r'GATC_y
Cuts at: 0 429 696 1693 1806
Size: 429 267 997 113
Fragments arranged by size:
997 429 267 113
BstZ17I GTA'TAC
Cuts at: 0 307 1806
Size: 307 1499
BtgI C'CryG_G
Cuts at: 0 187 1806
Size: 187 1619
BtsI GCAGTG_nn'
Cuts at: 0 1037 1806
Size: 1037 769
DraI TTT'AAA
Cuts at: 0 969 1182 1806
Size: 969 213 624
Fragments arranged by size:
969 624 213
DrdI GACnn_nn'nnGTC
Cuts at: 0 246 1806
Size: 246 1560
EaeI y'GGCC_r
Cuts at: 0 23 1557 1806
Size: 23 1534 249
Fragments arranged by size:
1534 249 23
EarI CTCTTCn'nnn_
Cuts at: 0 6 93 192 227 614 725 1332 1806
Size: 6 87 99 35 387 111 607 474
Fragments arranged by size:
607 474 387 111 99 87 35 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 1806
Size: 106 1700
EcoICRI GAG'CTC
Cuts at: 0 1063 1806
Size: 1063 743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 749 887 1313 1529 1726 1806
Size: 598 151 138 426 216 197 80
Fragments arranged by size:
598 426 216 197 151 138 80
EcoO109I rG'GnC_Cy
Cuts at: 0 631 1446 1806
Size: 631 815 360
Fragments arranged by size:
815 631 360
HaeII r_GCGC'y
Cuts at: 0 132 1806
Size: 132 1674
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 278 310 334 337 366 369 480 512
Size: 278 32 24 3 29 3 111 32
Cuts at: 512 847 879 1334 1366 1480 1512 1806
Size: 335 32 455 32 114 32 294
Fragments arranged by size:
455 335 294 278 114 111 32 32
32 32 32 29 24 3 3
HincII GTy'rAC
Cuts at: 0 332 1425 1806
Size: 332 1093 381
Fragments arranged by size:
1093 381 332
HindIII A'AGCT_T
Cuts at: 0 845 1806
Size: 845 961
KasI G'GCGC_C
Cuts at: 0 128 1806
Size: 128 1678
KpnI G_GTAC'C
Cuts at: 0 1660 1806
Size: 1660 146
MfeI C'AATT_G
Cuts at: 0 557 1806
Size: 557 1249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 1277 1806
Size: 392 885 529
Fragments arranged by size:
885 529 392
MslI CAynn'nnrTG
Cuts at: 0 741 1418 1806
Size: 741 677 388
Fragments arranged by size:
741 677 388
MspA1I CmG'CkG
Cuts at: 0 28 40 1781 1806
Size: 28 12 1741 25
Fragments arranged by size:
1741 28 25 12
NarI GG'CG_CC
Cuts at: 0 129 1806
Size: 129 1677
NdeI CA'TA_TG
Cuts at: 0 742 1806
Size: 742 1064
PflMI CCAn_nnn'nTGG
Cuts at: 0 1744 1806
Size: 1744 62
PpuMI rG'GwC_Cy
Cuts at: 0 1446 1806
Size: 1446 360
PvuII CAG'CTG
Cuts at: 0 1781 1806
Size: 1781 25
SacI G_AGCT'C
Cuts at: 0 1065 1806
Size: 1065 741
SapI GCTCTTCn'nnn_
Cuts at: 0 227 614 1806
Size: 227 387 1192
Fragments arranged by size:
1192 387 227
SfcI C'TryA_G
Cuts at: 0 280 1159 1806
Size: 280 879 647
Fragments arranged by size:
879 647 280
SfoI GGC'GCC
Cuts at: 0 130 1806
Size: 130 1676
SmlI C'TyrA_G
Cuts at: 0 536 980 1064 1416 1806
Size: 536 444 84 352 390
Fragments arranged by size:
536 444 390 352 84
TatI w'GTAC_w
Cuts at: 0 512 1227 1806
Size: 512 715 579
Fragments arranged by size:
715 579 512
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1539 1571 1806
Size: 1539 32 235
Fragments arranged by size:
1539 235 32
XhoI C'TCGA_G
Cuts at: 0 980 1806
Size: 980 826
XmnI GAAnn'nnTTC
Cuts at: 0 552 1806
Size: 552 1254
Enzymes that do cut:
AccI Acc65I AcuI AhdI AleI AlwNI ApoI AseI
AvaI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BmrI BpmI Bpu10I BpuEI BsaI BsaHI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BsrDI BsrGI BstBI BstYI BstZ17I BtgI BtsI DraI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII
Hin4I HincII HindIII KasI KpnI MfeI MmeI MslI
MspA1I NarI NdeI PflMI PpuMI PvuII SacI SapI
SfcI SfoI SmlI TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BcgI BfrBI BlpI Bme1580I BmgBI BmtI BplI BsaAI
BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspMI
BsrFI BssHII BssSI BstAPI BstEII BstXI Bsu36I BtgZI
ClaI CspCI DraIII EagI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HpaI MluI MscI NaeI NcoI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
Tth111I XbaI XcmI XmaI ZraI