Restriction map for
pFN21AE3427
(Linear) (Six-base) MAPSORT of: KIEE3427.seq Check: 1399 from: 1 to: 1806
KIEE3427.orf
With 182 enzymes: SgfI *
March 22, 2012 16:33 ..
AclI AA'CG_TT
Cuts at: 0 805 1806
Size: 805 1001
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 735 1255 1271 1806
Size: 249 486 520 16 535
Fragments arranged by size:
535 520 486 249 16
AflIII A'CryG_T
Cuts at: 0 1481 1806
Size: 1481 325
AhdI GACnn_n'nnGTC
Cuts at: 0 637 1806
Size: 637 1169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1516 1806
Size: 1516 290
AlwNI CAG_nnn'CTG
Cuts at: 0 232 304 1671 1806
Size: 232 72 1367 135
Fragments arranged by size:
1367 232 135 72
ApaI G_GGCC'C
Cuts at: 0 154 1806
Size: 154 1652
ApaLI G'TGCA_C
Cuts at: 0 1795 1806
Size: 1795 11
AvaI C'yCGr_G
Cuts at: 0 725 1806
Size: 725 1081
AvrII C'CTAG_G
Cuts at: 0 1576 1806
Size: 1576 230
BanI G'GyrC_C
Cuts at: 0 1279 1806
Size: 1279 527
BanII G_rGCy'C
Cuts at: 0 37 145 154 1513 1806
Size: 37 108 9 1359 293
Fragments arranged by size:
1359 293 108 37 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 314 1001 1806
Size: 314 687 805
Fragments arranged by size:
805 687 314
BciVI GTATCCnnnnn_n'
Cuts at: 0 1549 1806
Size: 1549 257
BclI T'GATC_A
Cuts at: 0 398 681 1806
Size: 398 283 1125
Fragments arranged by size:
1125 398 283
BfrBI ATG'CAT
Cuts at: 0 958 1806
Size: 958 848
BglI GCCn_nnn'nGGC
Cuts at: 0 149 1806
Size: 149 1657
BglII A'GATC_T
Cuts at: 0 16 1806
Size: 16 1790
BlpI GC'TnA_GC
Cuts at: 0 139 1806
Size: 139 1667
Bme1580I G_kGCm'C
Cuts at: 0 154 578 1209 1799 1806
Size: 154 424 631 590 7
Fragments arranged by size:
631 590 424 154 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 111 1070 1419 1806
Size: 111 959 349 387
Fragments arranged by size:
959 387 349 111
BmtI G_CTAG'C
Cuts at: 0 1585 1806
Size: 1585 221
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1628 1806
Size: 1628 178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 635 984 1038 1446 1806
Size: 211 424 349 54 408 360
Fragments arranged by size:
424 408 360 349 211 54
Bpu10I CC'TnA_GC
Cuts at: 0 31 1806
Size: 31 1775
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1806
Size: 640 1166
BsaI GGTCTCn'nnnn_
Cuts at: 0 960 1806
Size: 960 846
BsaBI GATnn'nnATC
Cuts at: 0 464 1806
Size: 464 1342
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 346 1103 1346 1458 1767 1770 1806
Size: 121 225 757 243 112 309 3 36
Fragments arranged by size:
757 309 243 225 121 112 36 3
BseYI C'CCAG_C
Cuts at: 0 1404 1806
Size: 1404 402
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 1806
Size: 685 1121
BsiHKAI G_wGCw'C
Cuts at: 0 37 45 220 1513 1691 1799 1806
Size: 37 8 175 1293 178 108 7
Fragments arranged by size:
1293 178 175 108 37 8 7
BsmI GAATG_Cn'
Cuts at: 0 958 1806
Size: 958 848
Bsp1286I G_dGCh'C
Cuts at: 0 37 45 145 154 220 578 1209 1513
Size: 37 8 100 9 66 358 631 304
Cuts at: 1513 1691 1799 1806
Size: 178 108 7
Fragments arranged by size:
631 358 304 178 108 100 66 37
9 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1679 1806
Size: 1679 127
BsrBI CCG'CTC
Cuts at: 0 249 1806
Size: 249 1557
BsrDI GCAATG_nn'
Cuts at: 0 358 491 564 1458 1806
Size: 358 133 73 894 348
Fragments arranged by size:
894 358 348 133 73
BsrFI r'CCGG_y
Cuts at: 0 1751 1806
Size: 1751 55
BstAPI GCAn_nnn'nTGC
Cuts at: 0 708 1453 1671 1695 1806
Size: 708 745 218 24 111
Fragments arranged by size:
745 708 218 111 24
BstBI TT'CG_AA
Cuts at: 0 992 1806
Size: 992 814
BstXI CCAn_nnnn'nTGG
Cuts at: 0 385 546 1806
Size: 385 161 1260
Fragments arranged by size:
1260 385 161
BstYI r'GATC_y
Cuts at: 0 16 459 1806
Size: 16 443 1347
Fragments arranged by size:
1347 443 16
BtgI C'CryG_G
Cuts at: 0 146 326 1806
Size: 146 180 1480
Fragments arranged by size:
1480 180 146
BtsI GCAGTG_nn'
Cuts at: 0 762 1806
Size: 762 1044
DraIII CAC_nnn'GTG
Cuts at: 0 1204 1806
Size: 1204 602
EaeI y'GGCC_r
Cuts at: 0 1398 1749 1806
Size: 1398 351 57
Fragments arranged by size:
1398 351 57
EcoICRI GAG'CTC
Cuts at: 0 35 1511 1806
Size: 35 1476 295
Fragments arranged by size:
1476 295 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 249 635 735 984 1038 1255 1271
Size: 211 38 386 100 249 54 217 16
Cuts at: 1271 1446 1806
Size: 175 360
Fragments arranged by size:
386 360 249 217 211 175 100 54
38 16
EcoO109I rG'GnC_Cy
Cuts at: 0 237 301 1409 1649 1806
Size: 237 64 1108 240 157
Fragments arranged by size:
1108 240 237 157 64
FspI TGC'GCA
Cuts at: 0 979 1806
Size: 979 827
FspAI rTGC'GCAy
Cuts at: 0 979 1806
Size: 979 827
HaeII r_GCGC'y
Cuts at: 0 139 1610 1806
Size: 139 1471 196
Fragments arranged by size:
1471 196 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 271 303 431 463 623 655
Size: 8 32 231 32 128 32 160 32
Cuts at: 655 1806
Size: 1151
Fragments arranged by size:
1151 231 160 128 32 32 32 32
8
HindIII A'AGCT_T
Cuts at: 0 658 1806
Size: 658 1148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1141 1667 1806
Size: 656 485 526 139
Fragments arranged by size:
656 526 485 139
MscI TGG'CCA
Cuts at: 0 1400 1806
Size: 1400 406
MslI CAynn'nnrTG
Cuts at: 0 439 484 625 1006 1699 1806
Size: 439 45 141 381 693 107
Fragments arranged by size:
693 439 381 141 107 45
MspA1I CmG'CkG
Cuts at: 0 148 1170 1404 1806
Size: 148 1022 234 402
Fragments arranged by size:
1022 402 234 148
NaeI GCC'GGC
Cuts at: 0 1753 1806
Size: 1753 53
NcoI C'CATG_G
Cuts at: 0 326 1806
Size: 326 1480
NdeI CA'TA_TG
Cuts at: 0 954 1806
Size: 954 852
NgoMIV G'CCGG_C
Cuts at: 0 1751 1806
Size: 1751 55
NheI G'CTAG_C
Cuts at: 0 1581 1806
Size: 1581 225
NruI TCG'CGA
Cuts at: 0 39 1806
Size: 39 1767
NsiI A_TGCA'T
Cuts at: 0 960 1806
Size: 960 846
NspI r_CATG'y
Cuts at: 0 49 1485 1503 1698 1806
Size: 49 1436 18 195 108
Fragments arranged by size:
1436 195 108 49 18
PasI CC'CwG_GG
Cuts at: 0 1240 1806
Size: 1240 566
PciI A'CATG_T
Cuts at: 0 1481 1806
Size: 1481 325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 239 811 843 1806
Size: 207 32 572 32 963
Fragments arranged by size:
963 572 207 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 237 1409 1806
Size: 237 1172 397
Fragments arranged by size:
1172 397 237
PspOMI G'GGCC_C
Cuts at: 0 150 1806
Size: 150 1656
PspXI vC'TCGA_Gb
Cuts at: 0 725 1806
Size: 725 1081
PstI C_TGCA'G
Cuts at: 0 24 300 1806
Size: 24 276 1506
Fragments arranged by size:
1506 276 24
PvuII CAG'CTG
Cuts at: 0 1170 1404 1806
Size: 1170 234 402
Fragments arranged by size:
1170 402 234
SacI G_AGCT'C
Cuts at: 0 37 1513 1806
Size: 37 1476 293
Fragments arranged by size:
1476 293 37
SacII CC_GC'GG
Cuts at: 0 149 1806
Size: 149 1657
ScaI AGT'ACT
Cuts at: 0 270 475 1806
Size: 270 205 1331
Fragments arranged by size:
1331 270 205
SfcI C'TryA_G
Cuts at: 0 20 296 1806
Size: 20 276 1510
Fragments arranged by size:
1510 276 20
SmlI C'TyrA_G
Cuts at: 0 655 725 1806
Size: 655 70 1081
Fragments arranged by size:
1081 655 70
SphI G_CATG'C
Cuts at: 0 1698 1806
Size: 1698 108
StyI C'CwwG_G
Cuts at: 0 326 1576 1806
Size: 326 1250 230
Fragments arranged by size:
1250 326 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 783 896 1731 1806
Size: 783 113 835 75
Fragments arranged by size:
835 783 113 75
TatI w'GTAC_w
Cuts at: 0 268 473 1806
Size: 268 205 1333
Fragments arranged by size:
1333 268 205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 546 672 1806
Size: 324 222 126 1134
Fragments arranged by size:
1134 324 222 126
XhoI C'TCGA_G
Cuts at: 0 725 1806
Size: 725 1081
XmnI GAAnn'nnTTC
Cuts at: 0 373 805 1806
Size: 373 432 1001
Fragments arranged by size:
1001 432 373
Enzymes that do cut:
AclI AcuI AflIII AhdI AlfI AlwNI ApaI ApaLI
AvaI AvrII BanI BanII BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstBI
BstXI BstYI BtgI BtsI DraIII EaeI EcoICRI Eco57MI
EcoO109I FspI FspAI HaeII Hin4I HindIII MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NruI
NsiI NspI PasI PciI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SacII ScaI SfcI SmlI SphI
StyI TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AleI
AloI ApoI AscI AseI AsiSI BaeI BamHI BbeI
BbvCI BcgI BmgBI BsaAI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EarI EciI EcoNI EcoRI EcoRV FalI FseI HincII
HpaI KasI KpnI MfeI MluI NarI NotI PacI
PflMI PfoI PmeI PmlI PshAI PsiI PsrI PvuI
RsrII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TstI Tth111I XbaI XmaI ZraI