Restriction map for
pFN21AE3424
(Linear) (Six-base) MAPSORT of: KIEE3424.seq Check: 7577 from: 1 to: 1512
KIEE3424.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 861 957 1512
Size: 861 96 555
Fragments arranged by size:
861 555 96
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1168 1512
Size: 1057 111 344
Fragments arranged by size:
1057 344 111
AfeI AGC'GCT
Cuts at: 0 1042 1512
Size: 1042 470
AflIII A'CryG_T
Cuts at: 0 1498 1512
Size: 1498 14
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 815 1512
Size: 815 697
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1180 1212 1512
Size: 1180 32 300
Fragments arranged by size:
1180 300 32
AlwNI CAG_nnn'CTG
Cuts at: 0 180 270 468 558 669 735 1386 1512
Size: 180 90 198 90 111 66 651 126
Fragments arranged by size:
651 198 180 126 111 90 90 66
ApaI G_GGCC'C
Cuts at: 0 163 241 1512
Size: 163 78 1271
Fragments arranged by size:
1271 163 78
AvaI C'yCGr_G
Cuts at: 0 162 361 891 1192 1512
Size: 162 199 530 301 320
Fragments arranged by size:
530 320 301 199 162
BanI G'GyrC_C
Cuts at: 0 217 844 924 1433 1512
Size: 217 627 80 509 79
Fragments arranged by size:
627 509 217 80 79
BanII G_rGCy'C
Cuts at: 0 163 241 368 611 964 1193 1400 1480
Size: 163 78 127 243 353 229 207 80
Cuts at: 1480 1512
Size: 32
Fragments arranged by size:
353 243 229 207 163 127 80 78
32
BbeI G_GCGC'C
Cuts at: 0 221 1437 1512
Size: 221 1216 75
Fragments arranged by size:
1216 221 75
BciVI GTATCCnnnnn_n'
Cuts at: 0 1060 1512
Size: 1060 452
BglI GCCn_nnn'nGGC
Cuts at: 0 563 1512
Size: 563 949
BlpI GC'TnA_GC
Cuts at: 0 772 1512
Size: 772 740
Bme1580I G_kGCm'C
Cuts at: 0 91 163 241 277 847 1512
Size: 91 72 78 36 570 665
Fragments arranged by size:
665 570 91 78 72 36
BmgBI CAC'GTC
Cuts at: 0 977 1512
Size: 977 535
BmrI ACTGGGnnnn_n'
Cuts at: 0 923 1512
Size: 923 589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1414 1465 1476 1512
Size: 445 969 51 11 36
Fragments arranged by size:
969 445 51 36 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 1094 1150 1512
Size: 1094 56 362
Fragments arranged by size:
1094 362 56
BsaAI yAC'GTr
Cuts at: 0 1499 1512
Size: 1499 13
BsaBI GATnn'nnATC
Cuts at: 0 1366 1512
Size: 1366 146
BsaHI Gr'CG_yC
Cuts at: 0 218 301 1434 1512
Size: 218 83 1133 78
Fragments arranged by size:
1133 218 83 78
BsaWI w'CCGG_w
Cuts at: 0 1115 1512
Size: 1115 397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1180 1210 1512
Size: 1180 30 302
Fragments arranged by size:
1180 302 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1470 1512
Size: 1470 42
BseYI C'CCAG_C
Cuts at: 0 193 270 693 1016 1379 1386 1512
Size: 193 77 423 323 363 7 126
Fragments arranged by size:
423 363 323 193 126 77 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 868 1512
Size: 364 504 644
Fragments arranged by size:
644 504 364
BsiEI CG_ry'CG
Cuts at: 0 107 1512
Size: 107 1405
BsiHKAI G_wGCw'C
Cuts at: 0 368 1400 1471 1512
Size: 368 1032 71 41
Fragments arranged by size:
1032 368 71 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 416 1512
Size: 416 1096
Bsp1286I G_dGCh'C
Cuts at: 0 91 163 241 277 368 611 847 964
Size: 91 72 78 36 91 243 236 117
Cuts at: 964 1193 1400 1471 1480 1512
Size: 229 207 71 9 32
Fragments arranged by size:
243 236 229 207 117 91 91 78
72 71 36 32 9
BspEI T'CCGG_A
Cuts at: 0 1115 1512
Size: 1115 397
BspHI T'CATG_A
Cuts at: 0 1421 1512
Size: 1421 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 861 957 1118 1512
Size: 861 96 161 394
Fragments arranged by size:
861 394 161 96
BsrBI CCG'CTC
Cuts at: 0 459 939 1512
Size: 459 480 573
Fragments arranged by size:
573 480 459
BsrFI r'CCGG_y
Cuts at: 0 102 106 356 915 1085 1512
Size: 102 4 250 559 170 427
Fragments arranged by size:
559 427 250 170 102 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 180 401 735 1077 1512
Size: 180 221 334 342 435
Fragments arranged by size:
435 342 334 221 180
BstEII G'GTnAC_C
Cuts at: 0 318 1512
Size: 318 1194
BstXI CCAn_nnnn'nTGG
Cuts at: 0 693 1011 1130 1512
Size: 693 318 119 382
Fragments arranged by size:
693 382 318 119
BtgI C'CryG_G
Cuts at: 0 213 314 813 834 1129 1512
Size: 213 101 499 21 295 383
Fragments arranged by size:
499 383 295 213 101 21
BtsI GCAGTG_nn'
Cuts at: 0 123 797 1512
Size: 123 674 715
Fragments arranged by size:
715 674 123
ClaI AT'CG_AT
Cuts at: 0 24 1512
Size: 24 1488
EaeI y'GGCC_r
Cuts at: 0 104 185 311 614 680 857 940 971
Size: 104 81 126 303 66 177 83 31
Cuts at: 971 1512
Size: 541
Fragments arranged by size:
541 303 177 126 104 83 81 66
31
EagI C'GGCC_G
Cuts at: 0 104 1512
Size: 104 1408
EarI CTCTTCn'nnn_
Cuts at: 0 1327 1512
Size: 1327 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 233 404 740 1200 1512
Size: 233 171 336 460 312
Fragments arranged by size:
460 336 312 233 171
EcoICRI GAG'CTC
Cuts at: 0 366 1398 1512
Size: 366 1032 114
Fragments arranged by size:
1032 366 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1057 1168 1414 1465 1476 1512
Size: 445 612 111 246 51 11 36
Fragments arranged by size:
612 445 246 111 51 36 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1164 1512
Size: 1164 348
EcoO109I rG'GnC_Cy
Cuts at: 0 159 237 238 1512
Size: 159 78 1 1274
Fragments arranged by size:
1274 159 78 1
FseI GG_CCGG'CC
Cuts at: 0 106 1512
Size: 106 1406
FspI TGC'GCA
Cuts at: 0 1070 1512
Size: 1070 442
HaeII r_GCGC'y
Cuts at: 0 221 558 1044 1437 1512
Size: 221 337 486 393 75
Fragments arranged by size:
486 393 337 221 75
KasI G'GCGC_C
Cuts at: 0 217 1433 1512
Size: 217 1216 79
Fragments arranged by size:
1216 217 79
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1130 1332 1512
Size: 277 853 202 180
Fragments arranged by size:
853 277 202 180
MscI TGG'CCA
Cuts at: 0 187 973 1512
Size: 187 786 539
Fragments arranged by size:
786 539 187
MslI CAynn'nnrTG
Cuts at: 0 325 394 427 589 802 1512
Size: 325 69 33 162 213 710
Fragments arranged by size:
710 325 213 162 69 33
MspA1I CmG'CkG
Cuts at: 0 215 223 286 295 378 468 575 678
Size: 215 8 63 9 83 90 107 103
Cuts at: 678 693 815 836 987 1030 1386 1512
Size: 15 122 21 151 43 356 126
Fragments arranged by size:
356 215 151 126 122 107 103 90
83 63 43 21 15 9 8
NaeI GCC'GGC
Cuts at: 0 104 108 1512
Size: 104 4 1404
Fragments arranged by size:
1404 104 4
NarI GG'CG_CC
Cuts at: 0 218 1434 1512
Size: 218 1216 78
Fragments arranged by size:
1216 218 78
NcoI C'CATG_G
Cuts at: 0 314 1512
Size: 314 1198
NgoMIV G'CCGG_C
Cuts at: 0 102 106 1512
Size: 102 4 1406
Fragments arranged by size:
1406 102 4
NruI TCG'CGA
Cuts at: 0 935 1512
Size: 935 577
NspI r_CATG'y
Cuts at: 0 399 1209 1512
Size: 399 810 303
Fragments arranged by size:
810 399 303
PflMI CCAn_nnn'nTGG
Cuts at: 0 270 1386 1512
Size: 270 1116 126
Fragments arranged by size:
1116 270 126
PfoI T'CCnGG_A
Cuts at: 0 338 1512
Size: 338 1174
PmlI CAC'GTG
Cuts at: 0 1499 1512
Size: 1499 13
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1180 1212 1512
Size: 1180 32 300
Fragments arranged by size:
1180 300 32
PspOMI G'GGCC_C
Cuts at: 0 159 237 1512
Size: 159 78 1275
Fragments arranged by size:
1275 159 78
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 613 645 1512
Size: 613 32 867
Fragments arranged by size:
867 613 32
PvuII CAG'CTG
Cuts at: 0 987 1386 1512
Size: 987 399 126
Fragments arranged by size:
987 399 126
RsrII CG'GwC_CG
Cuts at: 0 748 1512
Size: 748 764
SacI G_AGCT'C
Cuts at: 0 368 1400 1512
Size: 368 1032 112
Fragments arranged by size:
1032 368 112
SacII CC_GC'GG
Cuts at: 0 216 816 837 1512
Size: 216 600 21 675
Fragments arranged by size:
675 600 216 21
SfcI C'TryA_G
Cuts at: 0 630 1044 1512
Size: 630 414 468
Fragments arranged by size:
630 468 414
SfoI GGC'GCC
Cuts at: 0 219 1435 1512
Size: 219 1216 77
Fragments arranged by size:
1216 219 77
SmaI CCC'GGG
Cuts at: 0 164 893 1512
Size: 164 729 619
Fragments arranged by size:
729 619 164
SrfI GCCC'GGGC
Cuts at: 0 164 1512
Size: 164 1348
StyI C'CwwG_G
Cuts at: 0 314 1512
Size: 314 1198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 489 942 1512
Size: 489 453 570
Fragments arranged by size:
570 489 453
XmaI C'CCGG_G
Cuts at: 0 162 891 1512
Size: 162 729 621
Fragments arranged by size:
729 621 162
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AloI AlwNI ApaI
AvaI BanI BanII BbeI BciVI BglI BlpI Bme1580I
BmgBI BmrI BpmI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI
BtgI BtsI ClaI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI FspI HaeII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PflMI PfoI PmlI PpiI PspOMI PsrI PvuII
RsrII SacI SacII SfcI SfoI SmaI SrfI StyI
TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BclI BfrBI BglII BmtI BplI
Bpu10I BpuEI BsiWI BsmI BsrDI BsrGI BssHII BssSI
BstBI BstYI BstZ17I Bsu36I BtgZI CspCI DraI DraIII
DrdI EcoRI EcoRV FalI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NsiI
PacI PasI PciI PmeI PpuMI PshAI PsiI PspXI
PstI PvuI SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI