(Linear) (Six-base) MAPSORT of: KIEE3422.seq  Check: 5788  from: 1  to: 1479



KIEE3422.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:33  ..

Acc65I G'GTAC_C

Cuts at:      0     227    1479 
   Size:        227    1252
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     791    1479 
   Size:        791     688
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     813    1479 
   Size:        813     666
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1360    1392    1479 
   Size:       1360      32      87

  Fragments arranged by size: 

                1360      87      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     460    1450    1479 
   Size:        460     990      29

  Fragments arranged by size: 

                 990     460      29
 
ApoI r'AATT_y

Cuts at:      0     241    1479 
   Size:        241    1238
 
AseI AT'TA_AT

Cuts at:      0     332    1277    1400    1479 
   Size:        332     945     123      79

  Fragments arranged by size: 

                 945     332     123      79
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     211     244    1400    1433    1479 
   Size:        211      33    1156      33      46

  Fragments arranged by size: 

                1156     211      46      33      33
 
BanI G'GyrC_C

Cuts at:      0     227    1479 
   Size:        227    1252
 
BanII G_rGCy'C

Cuts at:      0     278     507     842    1479 
   Size:        278     229     335     637

  Fragments arranged by size: 

                 637     335     278     229
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1014    1479 
   Size:       1014     465
 
BclI T'GATC_A

Cuts at:      0     624    1479 
   Size:        624     855
 
BlpI GC'TnA_GC

Cuts at:      0     836    1479 
   Size:        836     643
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     399    1479 
   Size:        399    1080
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     629     728    1479 
   Size:        491     138      99     751

  Fragments arranged by size: 

                 751     491     138      99
 
Bpu10I CC'TnA_GC

Cuts at:      0     761    1479 
   Size:        761     718
 
BsaBI GATnn'nnATC

Cuts at:      0     623    1479 
   Size:        623     856
 
BseYI C'CCAG_C

Cuts at:      0    1270    1479 
   Size:       1270     209
 
BsiHKAI G_wGCw'C

Cuts at:      0     278     507     842    1479 
   Size:        278     229     335     637

  Fragments arranged by size: 

                 637     335     278     229
 
Bsp1286I G_dGCh'C

Cuts at:      0     278     507     842    1479 
   Size:        278     229     335     637

  Fragments arranged by size: 

                 637     335     278     229
 
BsrDI GCAATG_nn'

Cuts at:      0     149    1200    1479 
   Size:        149    1051     279

  Fragments arranged by size: 

                1051     279     149
 
DraI TTT'AAA

Cuts at:      0    1062    1479 
   Size:       1062     417
 
EaeI y'GGCC_r

Cuts at:      0      89    1479 
   Size:         89    1390
 
EcoICRI GAG'CTC

Cuts at:      0     276     505     840    1479 
   Size:        276     229     335     639

  Fragments arranged by size: 

                 639     335     276     229
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     629     728     791    1479 
   Size:        491     138      99      63     688

  Fragments arranged by size: 

                 688     491     138      99      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1423    1479 
   Size:       1423      56
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     120     816    1479 
   Size:        120     696     663

  Fragments arranged by size: 

                 696     663     120
 
HincII GTy'rAC

Cuts at:      0     249    1479 
   Size:        249    1230
 
KpnI G_GTAC'C

Cuts at:      0     231    1479 
   Size:        231    1248
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       9    1479 
   Size:          9    1470
 
MscI TGG'CCA

Cuts at:      0      91    1479 
   Size:         91    1388
 
MslI CAynn'nnrTG

Cuts at:      0    1402    1479 
   Size:       1402      77
 
MspA1I CmG'CkG

Cuts at:      0    1127    1479 
   Size:       1127     352
 
PfoI T'CCnGG_A

Cuts at:      0     606    1479 
   Size:        606     873
 
PpuMI rG'GwC_Cy

Cuts at:      0    1423    1479 
   Size:       1423      56
 
PsiI TTA'TAA

Cuts at:      0    1052    1394    1479 
   Size:       1052     342      85

  Fragments arranged by size: 

                1052     342      85
 
PstI C_TGCA'G

Cuts at:      0    1192    1263    1479 
   Size:       1192      71     216

  Fragments arranged by size: 

                1192     216      71
 
PvuII CAG'CTG

Cuts at:      0    1127    1479 
   Size:       1127     352
 
SacI G_AGCT'C

Cuts at:      0     278     507     842    1479 
   Size:        278     229     335     637

  Fragments arranged by size: 

                 637     335     278     229
 
SfcI C'TryA_G

Cuts at:      0     898    1188    1259    1479 
   Size:        898     290      71     220

  Fragments arranged by size: 

                 898     290     220      71
 
StyI C'CwwG_G

Cuts at:      0     667    1479 
   Size:        667     812
 
TatI w'GTAC_w

Cuts at:      0     909    1479 
   Size:        909     570
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      71     103    1203    1235    1479 
   Size:         71      32    1100      32     244

  Fragments arranged by size: 

                1100     244      71      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     567    1479 
   Size:        567     912
 
XmnI GAAnn'nnTTC

Cuts at:      0     930    1479 
   Size:        930     549
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI     AloI    AlwNI     ApoI     AseI     BaeI
     BanI    BanII    BciVI     BclI     BlpI     BmrI     BpmI   Bpu10I
    BsaBI    BseYI  BsiHKAI Bsp1286I    BsrDI     DraI     EaeI  EcoICRI
  Eco57MI EcoO109I     FalI   HincII     KpnI     MmeI     MscI     MslI
   MspA1I     PfoI    PpuMI     PsiI     PstI    PvuII     SacI     SfcI
     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
    BamHI     BbeI     BbsI    BbvCI     BcgI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EarI
     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     MfeI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     ZraI