Restriction map for
pFN21AE3419
(Linear) (Six-base) MAPSORT of: KIEE3419.seq Check: 6950 from: 1 to: 1425
KIEE3419.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 176 1425
Size: 176 1249
AccI GT'mk_AC
Cuts at: 0 620 1425
Size: 620 805
AfeI AGC'GCT
Cuts at: 0 566 1425
Size: 566 859
AgeI A'CCGG_T
Cuts at: 0 57 1425
Size: 57 1368
ApoI r'AATT_y
Cuts at: 0 1192 1425
Size: 1192 233
AvaI C'yCGr_G
Cuts at: 0 12 85 508 794 1338 1425
Size: 12 73 423 286 544 87
Fragments arranged by size:
544 423 286 87 73 12
AvrII C'CTAG_G
Cuts at: 0 264 1425
Size: 264 1161
BanI G'GyrC_C
Cuts at: 0 54 1425
Size: 54 1371
BanII G_rGCy'C
Cuts at: 0 574 1425
Size: 574 851
BbsI GAAGACnn'nnnn_
Cuts at: 0 282 1043 1425
Size: 282 761 382
Fragments arranged by size:
761 382 282
BbvCI CC'TCA_GC
Cuts at: 0 1362 1425
Size: 1362 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 813 847 1425
Size: 813 34 578
Fragments arranged by size:
813 578 34
Bme1580I G_kGCm'C
Cuts at: 0 456 1425
Size: 456 969
BmrI ACTGGGnnnn_n'
Cuts at: 0 282 1425
Size: 282 1143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 969 1315 1425
Size: 513 456 346 110
Fragments arranged by size:
513 456 346 110
Bpu10I CC'TnA_GC
Cuts at: 0 1362 1425
Size: 1362 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 649 769 1041 1425
Size: 637 12 120 272 384
Fragments arranged by size:
637 384 272 120 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 433 511 1141 1425
Size: 433 78 630 284
Fragments arranged by size:
630 433 284 78
BsaAI yAC'GTr
Cuts at: 0 552 624 1425
Size: 552 72 801
Fragments arranged by size:
801 552 72
BsaHI Gr'CG_yC
Cuts at: 0 1409 1425
Size: 1409 16
BsaWI w'CCGG_w
Cuts at: 0 57 1425
Size: 57 1368
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 967 997 1147 1177 1234 1264 1425
Size: 967 30 150 30 57 30 161
Fragments arranged by size:
967 161 150 57 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 435 553 772 1425
Size: 435 118 219 653
Fragments arranged by size:
653 435 219 118
BseYI C'CCAG_C
Cuts at: 0 123 567 819 1087 1378 1420 1425
Size: 123 444 252 268 291 42 5
Fragments arranged by size:
444 291 268 252 123 42 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 154 312 1063 1320 1425
Size: 24 130 158 751 257 105
Fragments arranged by size:
751 257 158 130 105 24
BsiEI CG_ry'CG
Cuts at: 0 369 1425
Size: 369 1056
BsiHKAI G_wGCw'C
Cuts at: 0 557 1425
Size: 557 868
Bsp1286I G_dGCh'C
Cuts at: 0 456 557 574 1425
Size: 456 101 17 851
Fragments arranged by size:
851 456 101 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 590 1425
Size: 176 414 835
Fragments arranged by size:
835 414 176
BsrFI r'CCGG_y
Cuts at: 0 51 57 501 1425
Size: 51 6 444 924
Fragments arranged by size:
924 444 51 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 600 819 1378 1425
Size: 600 219 559 47
Fragments arranged by size:
600 559 219 47
BstYI r'GATC_y
Cuts at: 0 1160 1216 1425
Size: 1160 56 209
Fragments arranged by size:
1160 209 56
BtgI C'CryG_G
Cuts at: 0 230 1097 1133 1212 1425
Size: 230 867 36 79 213
Fragments arranged by size:
867 230 213 79 36
BtsI GCAGTG_nn'
Cuts at: 0 317 1308 1320 1425
Size: 317 991 12 105
Fragments arranged by size:
991 317 105 12
DraI TTT'AAA
Cuts at: 0 924 1425
Size: 924 501
DrdI GACnn_nn'nnGTC
Cuts at: 0 675 1425
Size: 675 750
EaeI y'GGCC_r
Cuts at: 0 209 590 629 743 809 1425
Size: 209 381 39 114 66 616
Fragments arranged by size:
616 381 209 114 66 39
EarI CTCTTCn'nnn_
Cuts at: 0 562 1206 1425
Size: 562 644 219
Fragments arranged by size:
644 562 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 237 1321 1425
Size: 237 1084 104
Fragments arranged by size:
1084 237 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 969 1315 1425
Size: 513 456 346 110
Fragments arranged by size:
513 456 346 110
EcoNI CCTnn'n_nnAGG
Cuts at: 0 282 1155 1425
Size: 282 873 270
Fragments arranged by size:
873 282 270
EcoO109I rG'GnC_Cy
Cuts at: 0 102 261 648 1425
Size: 102 159 387 777
Fragments arranged by size:
777 387 159 102
EcoRV GAT'ATC
Cuts at: 0 946 1425
Size: 946 479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 783 1425
Size: 783 642
FspI TGC'GCA
Cuts at: 0 1126 1425
Size: 1126 299
FspAI rTGC'GCAy
Cuts at: 0 1126 1425
Size: 1126 299
HaeII r_GCGC'y
Cuts at: 0 568 752 1425
Size: 568 184 673
Fragments arranged by size:
673 568 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 411 443 1234 1260 1266 1292 1302 1334
Size: 411 32 791 26 6 26 10 32
Cuts at: 1334 1425
Size: 91
Fragments arranged by size:
791 411 91 32 32 26 26 10
6
HincII GTy'rAC
Cuts at: 0 198 1425
Size: 198 1227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 59 659 1425
Size: 50 9 600 766
Fragments arranged by size:
766 600 50 9
MscI TGG'CCA
Cuts at: 0 592 631 745 1425
Size: 592 39 114 680
Fragments arranged by size:
680 592 114 39
MslI CAynn'nnrTG
Cuts at: 0 136 379 1132 1425
Size: 136 243 753 293
Fragments arranged by size:
753 293 243 136
MspA1I CmG'CkG
Cuts at: 0 393 737 956 1378 1425
Size: 393 344 219 422 47
Fragments arranged by size:
422 393 344 219 47
NaeI GCC'GGC
Cuts at: 0 53 503 1425
Size: 53 450 922
Fragments arranged by size:
922 450 53
NgoMIV G'CCGG_C
Cuts at: 0 51 501 1425
Size: 51 450 924
Fragments arranged by size:
924 450 51
NspI r_CATG'y
Cuts at: 0 1063 1425
Size: 1063 362
PasI CC'CwG_GG
Cuts at: 0 1065 1425
Size: 1065 360
PflMI CCAn_nnn'nTGG
Cuts at: 0 1098 1425
Size: 1098 327
PpuMI rG'GwC_Cy
Cuts at: 0 261 1425
Size: 261 1164
PvuII CAG'CTG
Cuts at: 0 956 1378 1425
Size: 956 422 47
Fragments arranged by size:
956 422 47
SapI GCTCTTCn'nnn_
Cuts at: 0 562 1425
Size: 562 863
SfcI C'TryA_G
Cuts at: 0 1155 1385 1425
Size: 1155 230 40
Fragments arranged by size:
1155 230 40
SgrAI Cr'CCGG_yG
Cuts at: 0 57 1425
Size: 57 1368
SmaI CCC'GGG
Cuts at: 0 796 1340 1425
Size: 796 544 85
Fragments arranged by size:
796 544 85
SmlI C'TyrA_G
Cuts at: 0 652 664 784 1020 1425
Size: 652 12 120 236 405
Fragments arranged by size:
652 405 236 120 12
StuI AGG'CCT
Cuts at: 0 1006 1425
Size: 1006 419
StyI C'CwwG_G
Cuts at: 0 71 264 1425
Size: 71 193 1161
Fragments arranged by size:
1161 193 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 58 430 685 1425
Size: 58 372 255 740
Fragments arranged by size:
740 372 255 58
TatI w'GTAC_w
Cuts at: 0 608 1425
Size: 608 817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 1425
Size: 307 32 1086
Fragments arranged by size:
1086 307 32
Tth111I GACn'n_nGTC
Cuts at: 0 1272 1425
Size: 1272 153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1062 1095 1425
Size: 1062 33 330
Fragments arranged by size:
1062 330 33
XmaI C'CCGG_G
Cuts at: 0 794 1338 1425
Size: 794 544 87
Fragments arranged by size:
794 544 87
XmnI GAAnn'nnTTC
Cuts at: 0 21 1195 1425
Size: 21 1174 230
Fragments arranged by size:
1174 230 21
Enzymes that do cut:
AarI AccI AfeI AgeI ApoI AvaI AvrII BanI
BanII BbsI BbvCI BcgI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstXI BstYI
BtgI BtsI DraI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV FalI FspI FspAI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NgoMIV NspI
PasI PflMI PpuMI PvuII SapI SfcI SgrAI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BbeI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BsaBI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
EagI EcoICRI EcoRI FseI HindIII HpaI KasI KpnI
MfeI MluI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI ZraI