Restriction map for
pFN21AE3409
(Linear) (Six-base) MAPSORT of: KIEE3409.seq Check: 6765 from: 1 to: 915
KIEE3409.orf
With 182 enzymes: SgfI *
March 22, 2012 16:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 358 770 915
Size: 163 195 412 145
Fragments arranged by size:
412 195 163 145
AfeI AGC'GCT
Cuts at: 0 55 915
Size: 55 860
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 915
Size: 73 32 810
Fragments arranged by size:
810 73 32
AlwNI CAG_nnn'CTG
Cuts at: 0 75 549 804 892 915
Size: 75 474 255 88 23
Fragments arranged by size:
474 255 88 75 23
ApaLI G'TGCA_C
Cuts at: 0 579 915
Size: 579 336
AvaI C'yCGr_G
Cuts at: 0 26 915
Size: 26 889
AvrII C'CTAG_G
Cuts at: 0 660 915
Size: 660 255
BanII G_rGCy'C
Cuts at: 0 538 600 608 915
Size: 538 62 8 307
Fragments arranged by size:
538 307 62 8
BfrBI ATG'CAT
Cuts at: 0 204 521 915
Size: 204 317 394
Fragments arranged by size:
394 317 204
BlpI GC'TnA_GC
Cuts at: 0 269 915
Size: 269 646
Bme1580I G_kGCm'C
Cuts at: 0 583 915
Size: 583 332
BmrI ACTGGGnnnn_n'
Cuts at: 0 85 573 915
Size: 85 488 342
Fragments arranged by size:
488 342 85
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 627 915
Size: 627 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 915
Size: 889 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 915
Size: 427 488
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 915
Size: 73 30 812
Fragments arranged by size:
812 73 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 844 847 915
Size: 193 651 3 68
Fragments arranged by size:
651 193 68 3
BseYI C'CCAG_C
Cuts at: 0 185 387 572 885 915
Size: 185 202 185 313 30
Fragments arranged by size:
313 202 185 185 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 766 915
Size: 637 129 149
Fragments arranged by size:
637 149 129
BsiHKAI G_wGCw'C
Cuts at: 0 583 608 915
Size: 583 25 307
Fragments arranged by size:
583 307 25
BsmI GAATG_Cn'
Cuts at: 0 780 915
Size: 780 135
Bsp1286I G_dGCh'C
Cuts at: 0 538 583 600 608 915
Size: 538 45 17 8 307
Fragments arranged by size:
538 307 45 17 8
BsrBI CCG'CTC
Cuts at: 0 642 915
Size: 642 273
BsrDI GCAATG_nn'
Cuts at: 0 790 915
Size: 790 125
BssSI C'ACGA_G
Cuts at: 0 160 238 915
Size: 160 78 677
Fragments arranged by size:
677 160 78
BstYI r'GATC_y
Cuts at: 0 392 915
Size: 392 523
BtgI C'CryG_G
Cuts at: 0 821 915
Size: 821 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 190 915
Size: 190 725
ClaI AT'CG_AT
Cuts at: 0 635 915
Size: 635 280
EaeI y'GGCC_r
Cuts at: 0 894 915
Size: 894 21
EarI CTCTTCn'nnn_
Cuts at: 0 67 217 613 915
Size: 67 150 396 302
Fragments arranged by size:
396 302 150 67
EcoICRI GAG'CTC
Cuts at: 0 606 915
Size: 606 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 358 770 889 915
Size: 163 195 412 119 26
Fragments arranged by size:
412 195 163 119 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 915
Size: 361 554
EcoO109I rG'GnC_Cy
Cuts at: 0 697 915
Size: 697 218
HaeII r_GCGC'y
Cuts at: 0 57 877 915
Size: 57 820 38
Fragments arranged by size:
820 57 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 351 383 640 672 915
Size: 351 32 257 32 243
Fragments arranged by size:
351 257 243 32 32
MscI TGG'CCA
Cuts at: 0 896 915
Size: 896 19
MslI CAynn'nnrTG
Cuts at: 0 526 915
Size: 526 389
MspA1I CmG'CkG
Cuts at: 0 576 867 915
Size: 576 291 48
Fragments arranged by size:
576 291 48
NsiI A_TGCA'T
Cuts at: 0 206 523 915
Size: 206 317 392
Fragments arranged by size:
392 317 206
NspI r_CATG'y
Cuts at: 0 204 521 915
Size: 204 317 394
Fragments arranged by size:
394 317 204
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 892 915
Size: 75 817 23
Fragments arranged by size:
817 75 23
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 915
Size: 73 32 810
Fragments arranged by size:
810 73 32
PshAI GACnn'nnGTC
Cuts at: 0 649 915
Size: 649 266
PstI C_TGCA'G
Cuts at: 0 400 560 881 915
Size: 400 160 321 34
Fragments arranged by size:
400 321 160 34
PvuII CAG'CTG
Cuts at: 0 867 915
Size: 867 48
SacI G_AGCT'C
Cuts at: 0 608 915
Size: 608 307
SapI GCTCTTCn'nnn_
Cuts at: 0 613 915
Size: 613 302
SbfI CC_TGCA'GG
Cuts at: 0 560 915
Size: 560 355
SfcI C'TryA_G
Cuts at: 0 396 556 877 915
Size: 396 160 321 38
Fragments arranged by size:
396 321 160 38
SmlI C'TyrA_G
Cuts at: 0 406 915
Size: 406 509
SphI G_CATG'C
Cuts at: 0 521 915
Size: 521 394
StuI AGG'CCT
Cuts at: 0 280 404 915
Size: 280 124 511
Fragments arranged by size:
511 280 124
StyI C'CwwG_G
Cuts at: 0 422 660 915
Size: 422 238 255
Fragments arranged by size:
422 255 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 376 915
Size: 376 539
Enzymes that do cut:
AcuI AfeI AloI AlwNI ApaLI AvaI AvrII BanII
BfrBI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrDI
BssSI BstYI BtgI BtgZI ClaI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI MspA1I
NsiI NspI PflMI PpiI PshAI PstI PvuII SacI
SapI SbfI SfcI SmlI SphI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApoI AscI AseI AsiSI
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BmgBI BmtI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtsI CspCI DraI DraIII DrdI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI