(Linear) (Six-base) MAPSORT of: KIEE3403.seq  Check: 2947  from: 1  to: 510



KIEE3403.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     510 
   Size:         49     461
 
ApoI r'AATT_y

Cuts at:      0      76     405     510 
   Size:         76     329     105

  Fragments arranged by size: 

                 329     105      76
 
AseI AT'TA_AT

Cuts at:      0     332     510 
   Size:        332     178
 
BanI G'GyrC_C

Cuts at:      0     443     510 
   Size:        443      67
 
BbeI G_GCGC'C

Cuts at:      0     447     510 
   Size:        447      63
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      66     197     510 
   Size:         66     131     313

  Fragments arranged by size: 

                 313     131      66
 
BbvCI CC'TCA_GC

Cuts at:      0     479     510 
   Size:        479      31
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     421     455     510 
   Size:        421      34      55

  Fragments arranged by size: 

                 421      55      34
 
BmtI G_CTAG'C

Cuts at:      0     394     510 
   Size:        394     116
 
Bpu10I CC'TnA_GC

Cuts at:      0     479     510 
   Size:        479      31
 
BsaHI Gr'CG_yC

Cuts at:      0     444     510 
   Size:        444      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      83     113     510 
   Size:         83      30     397

  Fragments arranged by size: 

                 397      83      30
 
BseYI C'CCAG_C

Cuts at:      0     170     510 
   Size:        170     340
 
BtsI GCAGTG_nn'

Cuts at:      0      81     510 
   Size:         81     429
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     510 
   Size:         49     461
 
EcoRI G'AATT_C

Cuts at:      0     405     510 
   Size:        405     105
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      79     510 
   Size:         79     431
 
HaeII r_GCGC'y

Cuts at:      0     447     510 
   Size:        447      63
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      83     115     510 
   Size:         83      32     395

  Fragments arranged by size: 

                 395      83      32
 
KasI G'GCGC_C

Cuts at:      0     443     510 
   Size:        443      67
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     138     510 
   Size:        101      37     372

  Fragments arranged by size: 

                 372     101      37
 
MslI CAynn'nnrTG

Cuts at:      0     385     510 
   Size:        385     125
 
NarI GG'CG_CC

Cuts at:      0     444     510 
   Size:        444      66
 
NheI G'CTAG_C

Cuts at:      0     390     510 
   Size:        390     120
 
SfoI GGC'GCC

Cuts at:      0     445     510 
   Size:        445      65
 
XbaI T'CTAG_A

Cuts at:      0     177     510 
   Size:        177     333
 

 Enzymes that do cut:

     AcuI     ApoI     AseI     BanI     BbeI     BbsI    BbvCI     BcgI
     BmtI   Bpu10I    BsaHI    BsaXI    BseYI     BtsI  Eco57MI    EcoRI
     FalI    HaeII    Hin4I     KasI     MmeI     MslI     NarI     NheI
     SfoI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI