(Linear) (Six-base) MAPSORT of: KIEE3402.seq  Check: 829  from: 1  to: 447



KIEE3402.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     447 
   Size:        189     258
 
AvaI C'yCGr_G

Cuts at:      0      37     447 
   Size:         37     410
 
AvrII C'CTAG_G

Cuts at:      0     363     447 
   Size:        363      84
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79     447 
   Size:         79     368
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     447 
   Size:        189     258
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33     447 
   Size:         33     414
 
HindIII A'AGCT_T

Cuts at:      0     311     447 
   Size:        311     136
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     447 
   Size:        136     311
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      60     447 
   Size:         60     387
 
PfoI T'CCnGG_A

Cuts at:      0     299     447 
   Size:        299     148
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     124     156     447 
   Size:        124      32     291

  Fragments arranged by size: 

                 291     124      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      33     447 
   Size:         33     414
 
SfcI C'TryA_G

Cuts at:      0       9     143     447 
   Size:          9     134     304

  Fragments arranged by size: 

                 304     134       9
 
SmaI CCC'GGG

Cuts at:      0      39     447 
   Size:         39     408
 
StuI AGG'CCT

Cuts at:      0     415     447 
   Size:        415      32
 
StyI C'CwwG_G

Cuts at:      0     363     447 
   Size:        363      84
 
XmaI C'CCGG_G

Cuts at:      0      37     447 
   Size:         37     410
 

 Enzymes that do cut:

     AcuI     AvaI    AvrII    BseRI  Eco57MI EcoO109I  HindIII     MmeI
    PflMI     PfoI     PpiI    PpuMI     SfcI     SmaI     StuI     StyI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI