(Linear) (Six-base) MAPSORT of: KIEE3401.seq  Check: 1820  from: 1  to: 447



KIEE3401.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:31  ..

ApoI r'AATT_y

Cuts at:      0     221     447 
   Size:        221     226
 
AvaI C'yCGr_G

Cuts at:      0     419     447 
   Size:        419      28
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     243     277     447 
   Size:        243      34     170

  Fragments arranged by size: 

                 243     170      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     447 
   Size:         41     406
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      64     447 
   Size:         64     383
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     117     447 
   Size:        117     330
 
BstYI r'GATC_y

Cuts at:      0     380     447 
   Size:        380      67
 
Bsu36I CC'TnA_GG

Cuts at:      0     200     447 
   Size:        200     247
 
BtsI GCAGTG_nn'

Cuts at:      0     328     447 
   Size:        328     119
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     447 
   Size:         41     406
 
EcoO109I rG'GnC_Cy

Cuts at:      0     121     447 
   Size:        121     326
 
MslI CAynn'nnrTG

Cuts at:      0     115     333     447 
   Size:        115     218     114

  Fragments arranged by size: 

                 218     115     114
 
PpuMI rG'GwC_Cy

Cuts at:      0     121     447 
   Size:        121     326
 
PstI C_TGCA'G

Cuts at:      0      75     447 
   Size:         75     372
 
SfcI C'TryA_G

Cuts at:      0      71     176     447 
   Size:         71     105     271

  Fragments arranged by size: 

                 271     105      71
 
SspI AAT'ATT

Cuts at:      0     237     447 
   Size:        237     210
 
TatI w'GTAC_w

Cuts at:      0     404     447 
   Size:        404      43
 

 Enzymes that do cut:

     ApoI     AvaI     BcgI     BpmI    BspMI    BstXI    BstYI   Bsu36I
     BtsI  Eco57MI EcoO109I     MslI    PpuMI     PstI     SfcI     SspI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI