Restriction map for
pFN21AE3381
(Linear) (Six-base) MAPSORT of: KIEE3381.seq Check: 1229 from: 1 to: 3453
KIEE3381.orf
With 182 enzymes: SgfI *
July 3, 2012 17:48 ..
AccI GT'mk_AC
Cuts at: 0 348 3453
Size: 348 3105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1232 1523 1796 2070 2173 3100 3453
Size: 1232 291 273 274 103 927 353
Fragments arranged by size:
1232 927 353 291 274 273 103
AflIII A'CryG_T
Cuts at: 0 3444 3453
Size: 3444 9
AhdI GACnn_n'nnGTC
Cuts at: 0 246 455 3453
Size: 246 209 2998
Fragments arranged by size:
2998 246 209
ApaI G_GGCC'C
Cuts at: 0 1486 3453
Size: 1486 1967
ApaLI G'TGCA_C
Cuts at: 0 906 1693 2565 3453
Size: 906 787 872 888
Fragments arranged by size:
906 888 872 787
ApoI r'AATT_y
Cuts at: 0 145 338 951 979 2622 2760 3278 3453
Size: 145 193 613 28 1643 138 518 175
Fragments arranged by size:
1643 613 518 193 175 145 138 28
AseI AT'TA_AT
Cuts at: 0 1988 3453
Size: 1988 1465
AvaI C'yCGr_G
Cuts at: 0 929 3453
Size: 929 2524
BanII G_rGCy'C
Cuts at: 0 936 1151 1300 1421 1486 3453
Size: 936 215 149 121 65 1967
Fragments arranged by size:
1967 936 215 149 121 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 427 503 869 1220 3215 3453
Size: 427 76 366 351 1995 238
Fragments arranged by size:
1995 427 366 351 238 76
BbvCI CC'TCA_GC
Cuts at: 0 692 3200 3285 3453
Size: 692 2508 85 168
Fragments arranged by size:
2508 692 168 85
BclI T'GATC_A
Cuts at: 0 1040 3453
Size: 1040 2413
BglI GCCn_nnn'nGGC
Cuts at: 0 1109 3453
Size: 1109 2344
BglII A'GATC_T
Cuts at: 0 1544 2490 3453
Size: 1544 946 963
Fragments arranged by size:
1544 963 946
BlpI GC'TnA_GC
Cuts at: 0 2846 3453
Size: 2846 607
Bme1580I G_kGCm'C
Cuts at: 0 873 895 910 1486 1697 2486 2569 3453
Size: 873 22 15 576 211 789 83 884
Fragments arranged by size:
884 873 789 576 211 83 22 15
BmgBI CAC'GTC
Cuts at: 0 2481 2534 3453
Size: 2481 53 919
Fragments arranged by size:
2481 919 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 297 2012 3453
Size: 297 1715 1441
Fragments arranged by size:
1715 1441 297
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3285 3453
Size: 3285 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 328 1430 1950 2927 3453
Size: 290 38 1102 520 977 526
Fragments arranged by size:
1102 977 526 520 290 38
Bpu10I CC'TnA_GC
Cuts at: 0 431 692 2240 3200 3285 3453
Size: 431 261 1548 960 85 168
Fragments arranged by size:
1548 960 431 261 168 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 1061 1517 2303 3453
Size: 594 467 456 786 1150
Fragments arranged by size:
1150 786 594 467 456
BsaI GGTCTCn'nnnn_
Cuts at: 0 1350 3150 3453
Size: 1350 1800 303
Fragments arranged by size:
1800 1350 303
BsaBI GATnn'nnATC
Cuts at: 0 1685 3453
Size: 1685 1768
BsaWI w'CCGG_w
Cuts at: 0 843 3453
Size: 843 2610
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 88 118 1048 1078 2520 2550 3453
Size: 88 30 930 30 1442 30 903
Fragments arranged by size:
1442 930 903 88 30 30 30
BseYI C'CCAG_C
Cuts at: 0 1824 3453
Size: 1824 1629
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 2624 3453
Size: 644 1980 829
Fragments arranged by size:
1980 829 644
BsiEI CG_ry'CG
Cuts at: 0 43 3453
Size: 43 3410
BsiHKAI G_wGCw'C
Cuts at: 0 910 936 1151 1300 1697 2569 3453
Size: 910 26 215 149 397 872 884
Fragments arranged by size:
910 884 872 397 215 149 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2540 3453
Size: 2540 913
Bsp1286I G_dGCh'C
Cuts at: 0 873 895 910 936 1151 1300 1421 1486
Size: 873 22 15 26 215 149 121 65
Cuts at: 1486 1697 2486 2569 3453
Size: 211 789 83 884
Fragments arranged by size:
884 873 789 215 211 149 121 83
65 26 22 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3168 3453
Size: 3168 285
BsrBI CCG'CTC
Cuts at: 0 786 2523 3453
Size: 786 1737 930
Fragments arranged by size:
1737 930 786
BsrDI GCAATG_nn'
Cuts at: 0 2852 3453
Size: 2852 601
BsrFI r'CCGG_y
Cuts at: 0 2090 3362 3453
Size: 2090 1272 91
Fragments arranged by size:
2090 1272 91
BsrGI T'GTAC_A
Cuts at: 0 521 547 3453
Size: 521 26 2906
Fragments arranged by size:
2906 521 26
BssSI C'ACGA_G
Cuts at: 0 2560 3228 3453
Size: 2560 668 225
Fragments arranged by size:
2560 668 225
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2573 3453
Size: 2573 880
BstYI r'GATC_y
Cuts at: 0 1544 2490 3233 3453
Size: 1544 946 743 220
Fragments arranged by size:
1544 946 743 220
BtgI C'CryG_G
Cuts at: 0 704 1523 3453
Size: 704 819 1930
Fragments arranged by size:
1930 819 704
BtsI GCAGTG_nn'
Cuts at: 0 825 1025 1098 3453
Size: 825 200 73 2355
Fragments arranged by size:
2355 825 200 73
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 129 164 711 744 746 779 3453
Size: 129 35 547 33 2 33 2674
Fragments arranged by size:
2674 547 129 35 33 33 2
DraIII CAC_nnn'GTG
Cuts at: 0 961 2573 3453
Size: 961 1612 880
Fragments arranged by size:
1612 961 880
DrdI GACnn_nn'nnGTC
Cuts at: 0 2532 3453
Size: 2532 921
EaeI y'GGCC_r
Cuts at: 0 40 1797 3453
Size: 40 1757 1656
Fragments arranged by size:
1757 1656 40
EagI C'GGCC_G
Cuts at: 0 40 3453
Size: 40 3413
EarI CTCTTCn'nnn_
Cuts at: 0 572 2149 3453
Size: 572 1577 1304
Fragments arranged by size:
1577 1304 572
EcoICRI GAG'CTC
Cuts at: 0 934 1149 1298 3453
Size: 934 215 149 2155
Fragments arranged by size:
2155 934 215 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 328 1232 1430 1523 1796 1950 2070
Size: 290 38 904 198 93 273 154 120
Cuts at: 2070 2173 2927 3100 3453
Size: 103 754 173 353
Fragments arranged by size:
904 754 353 290 273 198 173 154
120 103 93 38
EcoO109I rG'GnC_Cy
Cuts at: 0 435 1483 3453
Size: 435 1048 1970
Fragments arranged by size:
1970 1048 435
EcoRI G'AATT_C
Cuts at: 0 951 979 3278 3453
Size: 951 28 2299 175
Fragments arranged by size:
2299 951 175 28
EcoRV GAT'ATC
Cuts at: 0 1518 2367 3375 3453
Size: 1518 849 1008 78
Fragments arranged by size:
1518 1008 849 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 417 1412 3453
Size: 417 995 2041
Fragments arranged by size:
2041 995 417
FspI TGC'GCA
Cuts at: 0 2200 3453
Size: 2200 1253
FspAI rTGC'GCAy
Cuts at: 0 2200 3453
Size: 2200 1253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 232 264 414 441 446 473
Size: 88 32 112 32 150 27 5 27
Cuts at: 473 592 624 1282 1314 2351 2383 2520 2552
Size: 119 32 658 32 1037 32 137 32
Cuts at: 2552 3205 3237 3453
Size: 653 32 216
Fragments arranged by size:
1037 658 653 216 150 137 119 112
88 32 32 32 32 32 32 32
27 27 5
HincII GTy'rAC
Cuts at: 0 349 2665 3453
Size: 349 2316 788
Fragments arranged by size:
2316 788 349
HindIII A'AGCT_T
Cuts at: 0 132 1389 1535 1778 2069 3453
Size: 132 1257 146 243 291 1384
Fragments arranged by size:
1384 1257 291 243 146 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2192 3367 3453
Size: 2192 1175 86
Fragments arranged by size:
2192 1175 86
MscI TGG'CCA
Cuts at: 0 1799 3453
Size: 1799 1654
MslI CAynn'nnrTG
Cuts at: 0 2456 3453
Size: 2456 997
MspA1I CmG'CkG
Cuts at: 0 684 1828 3453
Size: 684 1144 1625
Fragments arranged by size:
1625 1144 684
NaeI GCC'GGC
Cuts at: 0 2092 3364 3453
Size: 2092 1272 89
Fragments arranged by size:
2092 1272 89
NcoI C'CATG_G
Cuts at: 0 704 1523 3453
Size: 704 819 1930
Fragments arranged by size:
1930 819 704
NgoMIV G'CCGG_C
Cuts at: 0 2090 3362 3453
Size: 2090 1272 91
Fragments arranged by size:
2090 1272 91
NspI r_CATG'y
Cuts at: 0 3436 3448 3453
Size: 3436 12 5
Fragments arranged by size:
3436 12 5
PciI A'CATG_T
Cuts at: 0 3444 3453
Size: 3444 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 2953 3349 3453
Size: 2953 396 104
Fragments arranged by size:
2953 396 104
PfoI T'CCnGG_A
Cuts at: 0 215 2373 3453
Size: 215 2158 1080
Fragments arranged by size:
2158 1080 215
PpuMI rG'GwC_Cy
Cuts at: 0 435 3453
Size: 435 3018
PshAI GACnn'nnGTC
Cuts at: 0 2660 3453
Size: 2660 793
PsiI TTA'TAA
Cuts at: 0 3403 3453
Size: 3403 50
PspOMI G'GGCC_C
Cuts at: 0 1482 3453
Size: 1482 1971
PspXI vC'TCGA_Gb
Cuts at: 0 929 3453
Size: 929 2524
PstI C_TGCA'G
Cuts at: 0 797 820 3453
Size: 797 23 2633
Fragments arranged by size:
2633 797 23
PvuII CAG'CTG
Cuts at: 0 684 1828 3453
Size: 684 1144 1625
Fragments arranged by size:
1625 1144 684
SacI G_AGCT'C
Cuts at: 0 936 1151 1300 3453
Size: 936 215 149 2153
Fragments arranged by size:
2153 936 215 149
SalI G'TCGA_C
Cuts at: 0 347 3453
Size: 347 3106
SapI GCTCTTCn'nnn_
Cuts at: 0 572 3453
Size: 572 2881
SfcI C'TryA_G
Cuts at: 0 793 816 1017 3453
Size: 793 23 201 2436
Fragments arranged by size:
2436 793 201 23
SmlI C'TyrA_G
Cuts at: 0 609 929 1076 1532 2318 3453
Size: 609 320 147 456 786 1135
Fragments arranged by size:
1135 786 609 456 320 147
SspI AAT'ATT
Cuts at: 0 1461 3358 3453
Size: 1461 1897 95
Fragments arranged by size:
1897 1461 95
StuI AGG'CCT
Cuts at: 0 2256 3453
Size: 2256 1197
StyI C'CwwG_G
Cuts at: 0 704 1523 1634 3453
Size: 704 819 111 1819
Fragments arranged by size:
1819 819 704 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2369 3056 3453
Size: 2369 687 397
Fragments arranged by size:
2369 687 397
TatI w'GTAC_w
Cuts at: 0 521 547 809 3453
Size: 521 26 262 2644
Fragments arranged by size:
2644 521 262 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 3453
Size: 1048 32 2373
Fragments arranged by size:
2373 1048 32
Tth111I GACn'n_nGTC
Cuts at: 0 2532 3453
Size: 2532 921
XbaI T'CTAG_A
Cuts at: 0 936 3453
Size: 936 2517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 468 2547 3453
Size: 468 2079 906
Fragments arranged by size:
2079 906 468
XhoI C'TCGA_G
Cuts at: 0 929 3453
Size: 929 2524
Enzymes that do cut:
AccI AcuI AflIII AhdI ApaI ApaLI ApoI AseI
AvaI BanII BbsI BbvCI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstYI BtgI BtsI CspCI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
FspAI Hin4I HincII HindIII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PciI PflMI PfoI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuII SacI SalI
SapI SfcI SmlI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI AscI AsiSI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BfrBI BmtI BsaAI BsaHI
BseRI BsiWI BsmI BspEI BspHI BssHII BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI DraI EciI EcoNI
FseI HaeII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PsrI PvuI RsrII SacII SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI XmaI XmnI ZraI