Restriction map for
pFN21AE3371
(Linear) (Six-base) MAPSORT of: KIEE3371.seq Check: 637 from: 1 to: 3306
KIEE3371.orf
With 182 enzymes: SgfI *
February 3, 2015 16:28 ..
AatII G_ACGT'C
Cuts at: 0 485 494 610 2276 3306
Size: 485 9 116 1666 1030
Fragments arranged by size:
1666 1030 485 116 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2065 2771 3306
Size: 2065 706 535
Fragments arranged by size:
2065 706 535
AfeI AGC'GCT
Cuts at: 0 233 2384 3306
Size: 233 2151 922
Fragments arranged by size:
2151 922 233
AflII C'TTAA_G
Cuts at: 0 1747 3306
Size: 1747 1559
AflIII A'CryG_T
Cuts at: 0 1448 3306
Size: 1448 1858
AleI CACnn'nnGTG
Cuts at: 0 1040 3306
Size: 1040 2266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2197 3306
Size: 2197 1109
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1525 1557 3306
Size: 1525 32 1749
Fragments arranged by size:
1749 1525 32
AlwNI CAG_nnn'CTG
Cuts at: 0 354 1890 1941 3306
Size: 354 1536 51 1365
Fragments arranged by size:
1536 1365 354 51
ApaLI G'TGCA_C
Cuts at: 0 502 3306
Size: 502 2804
ApoI r'AATT_y
Cuts at: 0 2002 2325 2669 3022 3306
Size: 2002 323 344 353 284
Fragments arranged by size:
2002 353 344 323 284
AseI AT'TA_AT
Cuts at: 0 2933 3306
Size: 2933 373
AvaI C'yCGr_G
Cuts at: 0 393 3306
Size: 393 2913
BanI G'GyrC_C
Cuts at: 0 56 419 724 3306
Size: 56 363 305 2582
Fragments arranged by size:
2582 363 305 56
BanII G_rGCy'C
Cuts at: 0 110 160 394 1281 3306
Size: 110 50 234 887 2025
Fragments arranged by size:
2025 887 234 110 50
BbeI G_GCGC'C
Cuts at: 0 60 728 3306
Size: 60 668 2578
Fragments arranged by size:
2578 668 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 1481 3007 3143 3306
Size: 1481 1526 136 163
Fragments arranged by size:
1526 1481 163 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 645 679 1566 1600 3306
Size: 645 34 887 34 1706
Fragments arranged by size:
1706 887 645 34 34
BclI T'GATC_A
Cuts at: 0 2858 3080 3207 3306
Size: 2858 222 127 99
Fragments arranged by size:
2858 222 127 99
BglII A'GATC_T
Cuts at: 0 323 1295 3306
Size: 323 972 2011
Fragments arranged by size:
2011 972 323
Bme1580I G_kGCm'C
Cuts at: 0 506 2900 3306
Size: 506 2394 406
Fragments arranged by size:
2394 506 406
BmgBI CAC'GTC
Cuts at: 0 1508 3306
Size: 1508 1798
BmrI ACTGGGnnnn_n'
Cuts at: 0 1343 2106 3114 3306
Size: 1343 763 1008 192
Fragments arranged by size:
1343 1008 763 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 256 538 914 1276 1298 1963 3306
Size: 28 228 282 376 362 22 665 1343
Fragments arranged by size:
1343 665 376 362 282 228 28 22
Bpu10I CC'TnA_GC
Cuts at: 0 1589 1769 3306
Size: 1589 180 1537
Fragments arranged by size:
1589 1537 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1261 2115 2458 3306
Size: 874 387 854 343 848
Fragments arranged by size:
874 854 848 387 343
BsaI GGTCTCn'nnnn_
Cuts at: 0 232 708 3306
Size: 232 476 2598
Fragments arranged by size:
2598 476 232
BsaAI yAC'GTr
Cuts at: 0 180 912 3306
Size: 180 732 2394
Fragments arranged by size:
2394 732 180
BsaHI Gr'CG_yC
Cuts at: 0 57 482 491 607 725 2273 2651 3306
Size: 57 425 9 116 118 1548 378 655
Fragments arranged by size:
1548 655 425 378 118 116 57 9
BsaWI w'CCGG_w
Cuts at: 0 386 3306
Size: 386 2920
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 412 442 616 646 1210 1240 1274 1304
Size: 412 30 174 30 564 30 34 30
Cuts at: 1304 1424 1454 3306
Size: 120 30 1852
Fragments arranged by size:
1852 564 412 174 120 34 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 1231 3196 3306
Size: 448 783 1965 110
Fragments arranged by size:
1965 783 448 110
BseYI C'CCAG_C
Cuts at: 0 270 3306
Size: 270 3036
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 367 433 969 2170 2816 3265 3306
Size: 160 207 66 536 1201 646 449 41
Fragments arranged by size:
1201 646 536 449 207 160 66 41
BsiEI CG_ry'CG
Cuts at: 0 1074 3306
Size: 1074 2232
BsiHKAI G_wGCw'C
Cuts at: 0 110 506 3306
Size: 110 396 2800
Fragments arranged by size:
2800 396 110
BsmI GAATG_Cn'
Cuts at: 0 3119 3306
Size: 3119 187
Bsp1286I G_dGCh'C
Cuts at: 0 110 160 394 506 1281 2900 3306
Size: 110 50 234 112 775 1619 406
Fragments arranged by size:
1619 775 406 234 112 110 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1403 2327 3306
Size: 1403 924 979
Fragments arranged by size:
1403 979 924
BsrDI GCAATG_nn'
Cuts at: 0 1925 3306
Size: 1925 1381
BsrFI r'CCGG_y
Cuts at: 0 184 266 997 3306
Size: 184 82 731 2309
Fragments arranged by size:
2309 731 184 82
BssSI C'ACGA_G
Cuts at: 0 1036 3306
Size: 1036 2270
BstEII G'GTnAC_C
Cuts at: 0 540 3306
Size: 540 2766
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1557 3306
Size: 1557 1749
BstYI r'GATC_y
Cuts at: 0 323 401 1295 2580 3306
Size: 323 78 894 1285 726
Fragments arranged by size:
1285 894 726 323 78
BtgI C'CryG_G
Cuts at: 0 26 101 189 531 598 1023 1428 3306
Size: 26 75 88 342 67 425 405 1878
Fragments arranged by size:
1878 425 405 342 88 75 67 26
BtsI GCAGTG_nn'
Cuts at: 0 84 319 882 2777 3306
Size: 84 235 563 1895 529
Fragments arranged by size:
1895 563 529 235 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2643 2678 3306
Size: 2643 35 628
Fragments arranged by size:
2643 628 35
DraI TTT'AAA
Cuts at: 0 2445 2496 2706 3306
Size: 2445 51 210 600
Fragments arranged by size:
2445 600 210 51
DraIII CAC_nnn'GTG
Cuts at: 0 411 3306
Size: 411 2895
EaeI y'GGCC_r
Cuts at: 0 86 182 186 563 755 825 967 1811
Size: 86 96 4 377 192 70 142 844
Cuts at: 1811 1832 3306
Size: 21 1474
Fragments arranged by size:
1474 844 377 192 142 96 86 70
21 4
EarI CTCTTCn'nnn_
Cuts at: 0 896 956 3306
Size: 896 60 2350
Fragments arranged by size:
2350 896 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 268 571 3306
Size: 268 303 2735
Fragments arranged by size:
2735 303 268
EcoICRI GAG'CTC
Cuts at: 0 108 3306
Size: 108 3198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 256 538 914 1276 1298 1963 2065
Size: 28 228 282 376 362 22 665 102
Cuts at: 2065 2771 3306
Size: 706 535
Fragments arranged by size:
706 665 535 376 362 282 228 102
28 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 946 2160 3306
Size: 946 1214 1146
Fragments arranged by size:
1214 1146 946
EcoRI G'AATT_C
Cuts at: 0 2325 3306
Size: 2325 981
EcoRV GAT'ATC
Cuts at: 0 1107 1460 3306
Size: 1107 353 1846
Fragments arranged by size:
1846 1107 353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1393 2254 2542 3306
Size: 1393 861 288 764
Fragments arranged by size:
1393 861 764 288
FseI GG_CCGG'CC
Cuts at: 0 188 3306
Size: 188 3118
HaeII r_GCGC'y
Cuts at: 0 60 172 235 469 728 2027 2386 3306
Size: 60 112 63 234 259 1299 359 920
Fragments arranged by size:
1299 920 359 259 234 112 63 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1424 1456 2233 2265 2557 2589 3306
Size: 1424 32 777 32 292 32 717
Fragments arranged by size:
1424 777 717 292 32 32 32
HincII GTy'rAC
Cuts at: 0 1877 3125 3306
Size: 1877 1248 181
Fragments arranged by size:
1877 1248 181
HindIII A'AGCT_T
Cuts at: 0 1485 2308 2343 3306
Size: 1485 823 35 963
Fragments arranged by size:
1485 963 823 35
HpaI GTT'AAC
Cuts at: 0 1877 3125 3306
Size: 1877 1248 181
Fragments arranged by size:
1877 1248 181
KasI G'GCGC_C
Cuts at: 0 56 724 3306
Size: 56 668 2582
Fragments arranged by size:
2582 668 56
MfeI C'AATT_G
Cuts at: 0 1660 1825 1984 2390 2480 2660 3306
Size: 1660 165 159 406 90 180 646
Fragments arranged by size:
1660 646 406 180 165 159 90
MscI TGG'CCA
Cuts at: 0 565 757 1813 1834 3306
Size: 565 192 1056 21 1472
Fragments arranged by size:
1472 1056 565 192 21
MslI CAynn'nnrTG
Cuts at: 0 509 751 1040 2179 2504 3306
Size: 509 242 289 1139 325 802
Fragments arranged by size:
1139 802 509 325 289 242
MspA1I CmG'CkG
Cuts at: 0 417 453 972 3306
Size: 417 36 519 2334
Fragments arranged by size:
2334 519 417 36
NaeI GCC'GGC
Cuts at: 0 186 3306
Size: 186 3120
NarI GG'CG_CC
Cuts at: 0 57 725 3306
Size: 57 668 2581
Fragments arranged by size:
2581 668 57
NcoI C'CATG_G
Cuts at: 0 101 3306
Size: 101 3205
NdeI CA'TA_TG
Cuts at: 0 2358 2598 3306
Size: 2358 240 708
Fragments arranged by size:
2358 708 240
NgoMIV G'CCGG_C
Cuts at: 0 184 3306
Size: 184 3122
NspI r_CATG'y
Cuts at: 0 1452 2526 3306
Size: 1452 1074 780
Fragments arranged by size:
1452 1074 780
PciI A'CATG_T
Cuts at: 0 1448 3306
Size: 1448 1858
PflMI CCAn_nnn'nTGG
Cuts at: 0 270 1830 3306
Size: 270 1560 1476
Fragments arranged by size:
1560 1476 270
PfoI T'CCnGG_A
Cuts at: 0 620 1889 2364 3152 3306
Size: 620 1269 475 788 154
Fragments arranged by size:
1269 788 620 475 154
PmlI CAC'GTG
Cuts at: 0 180 912 3306
Size: 180 732 2394
Fragments arranged by size:
2394 732 180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 1030 1062 3306
Size: 505 32 493 32 2244
Fragments arranged by size:
2244 505 493 32 32
PstI C_TGCA'G
Cuts at: 0 737 1341 2324 3306
Size: 737 604 983 982
Fragments arranged by size:
983 982 737 604
SacI G_AGCT'C
Cuts at: 0 110 3306
Size: 110 3196
SexAI A'CCwGG_T
Cuts at: 0 338 407 839 3306
Size: 338 69 432 2467
Fragments arranged by size:
2467 432 338 69
SfcI C'TryA_G
Cuts at: 0 733 1337 2320 2459 3306
Size: 733 604 983 139 847
Fragments arranged by size:
983 847 733 604 139
SfoI GGC'GCC
Cuts at: 0 58 726 3306
Size: 58 668 2580
Fragments arranged by size:
2580 668 58
SmaI CCC'GGG
Cuts at: 0 395 3306
Size: 395 2911
SmlI C'TyrA_G
Cuts at: 0 889 1240 1747 2094 2437 3306
Size: 889 351 507 347 343 869
Fragments arranged by size:
889 869 507 351 347 343
SrfI GCCC'GGGC
Cuts at: 0 395 3306
Size: 395 2911
SspI AAT'ATT
Cuts at: 0 2853 3306
Size: 2853 453
StuI AGG'CCT
Cuts at: 0 1333 3306
Size: 1333 1973
StyI C'CwwG_G
Cuts at: 0 101 1361 1479 3306
Size: 101 1260 118 1827
Fragments arranged by size:
1827 1260 118 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 591 864 1769 3306
Size: 591 273 905 1537
Fragments arranged by size:
1537 905 591 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 412 444 616 648 1210 1242 3227 3259
Size: 412 32 172 32 562 32 1985 32
Cuts at: 3259 3306
Size: 47
Fragments arranged by size:
1985 562 412 172 47 32 32 32
32
XmaI C'CCGG_G
Cuts at: 0 393 3306
Size: 393 2913
XmnI GAAnn'nnTTC
Cuts at: 0 905 2759 3306
Size: 905 1854 547
Fragments arranged by size:
1854 905 547
ZraI GAC'GTC
Cuts at: 0 483 492 608 2274 3306
Size: 483 9 116 1666 1032
Fragments arranged by size:
1666 1032 483 116 9
Enzymes that do cut:
AatII AcuI AfeI AflII AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AseI AvaI BanI BanII BbeI
BbsI BcgI BclI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrFI BssSI BstEII BstXI BstYI BtgI BtsI CspCI
DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FseI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmlI
PpiI PstI SacI SexAI SfcI SfoI SmaI SmlI
SrfI SspI StuI StyI TaqII TstI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AgeI AhdI ApaI AscI
AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BglI
BlpI BmtI BplI BsaBI BsiWI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI DrdI EagI EcoO109I FspI FspAI KpnI MluI
MmeI NheI NotI NruI NsiI PacI PasI PmeI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SphI SwaI TatI Tth111I
XbaI XcmI XhoI