Restriction map for
pFN21AE3368
(Linear) (Six-base) MAPSORT of: KIEE3368.seq Check: 183 from: 1 to: 3264
KIEE3368.orf
With 182 enzymes: SgfI *
July 3, 2012 17:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 779 1571 3264
Size: 779 792 1693
Fragments arranged by size:
1693 792 779
Acc65I G'GTAC_C
Cuts at: 0 562 3264
Size: 562 2702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2581 2788 2901 2920 3158 3260 3264
Size: 2581 207 113 19 238 102 4
Fragments arranged by size:
2581 238 207 113 102 19 4
AlwNI CAG_nnn'CTG
Cuts at: 0 54 318 1038 3264
Size: 54 264 720 2226
Fragments arranged by size:
2226 720 264 54
ApaI G_GGCC'C
Cuts at: 0 580 671 1379 1642 3264
Size: 580 91 708 263 1622
Fragments arranged by size:
1622 708 580 263 91
ApoI r'AATT_y
Cuts at: 0 3000 3115 3264
Size: 3000 115 149
Fragments arranged by size:
3000 149 115
AvaI C'yCGr_G
Cuts at: 0 429 1651 2644 3264
Size: 429 1222 993 620
Fragments arranged by size:
1222 993 620 429
BanI G'GyrC_C
Cuts at: 0 274 562 706 777 895 1161 1395 1492
Size: 274 288 144 71 118 266 234 97
Cuts at: 1492 1535 2029 2600 2742 3264
Size: 43 494 571 142 522
Fragments arranged by size:
571 522 494 288 274 266 234 144
142 118 97 71 43
BanII G_rGCy'C
Cuts at: 0 360 485 580 671 932 997 1271 1379
Size: 360 125 95 91 261 65 274 108
Cuts at: 1379 1642 1652 2513 2824 3205 3264
Size: 263 10 861 311 381 59
Fragments arranged by size:
861 381 360 311 274 263 261 125
108 95 91 65 59 10
BbeI G_GCGC'C
Cuts at: 0 899 1165 1496 3264
Size: 899 266 331 1768
Fragments arranged by size:
1768 899 331 266
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 2095 2277 2569 3264
Size: 86 2009 182 292 695
Fragments arranged by size:
2009 695 292 182 86
BbvCI CC'TCA_GC
Cuts at: 0 1348 3264
Size: 1348 1916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 46 80 366 400 520 554 1545 1579
Size: 46 34 286 34 120 34 991 34
Cuts at: 1579 3264
Size: 1685
Fragments arranged by size:
1685 991 286 120 46 34 34 34
34
BclI T'GATC_A
Cuts at: 0 2774 3264
Size: 2774 490
BglI GCCn_nnn'nGGC
Cuts at: 0 1123 3264
Size: 1123 2141
BglII A'GATC_T
Cuts at: 0 2397 3264
Size: 2397 867
BlpI GC'TnA_GC
Cuts at: 0 556 1916 2818 3264
Size: 556 1360 902 446
Fragments arranged by size:
1360 902 556 446
Bme1580I G_kGCm'C
Cuts at: 0 277 580 671 732 1208 1379 1400 1540
Size: 277 303 91 61 476 171 21 140
Cuts at: 1540 1642 3264
Size: 102 1622
Fragments arranged by size:
1622 476 303 277 171 140 102 91
61 21
BmgBI CAC'GTC
Cuts at: 0 1509 2136 3264
Size: 1509 627 1128
Fragments arranged by size:
1509 1128 627
BmrI ACTGGGnnnn_n'
Cuts at: 0 490 1195 2450 2790 3264
Size: 490 705 1255 340 474
Fragments arranged by size:
1255 705 490 474 340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2722 3264
Size: 2722 542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 631 1177 1900 2919 3264
Size: 418 213 546 723 1019 345
Fragments arranged by size:
1019 723 546 418 345 213
Bpu10I CC'TnA_GC
Cuts at: 0 1348 2291 3098 3264
Size: 1348 943 807 166
Fragments arranged by size:
1348 943 807 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 346 3264
Size: 249 97 2918
Fragments arranged by size:
2918 249 97
BsaI GGTCTCn'nnnn_
Cuts at: 0 838 3264
Size: 838 2426
BsaAI yAC'GTr
Cuts at: 0 633 936 1179 1827 3261 3264
Size: 633 303 243 648 1434 3
Fragments arranged by size:
1434 648 633 303 243 3
BsaHI Gr'CG_yC
Cuts at: 0 835 896 1162 1454 1493 3264
Size: 835 61 266 292 39 1771
Fragments arranged by size:
1771 835 292 266 61 39
BsaWI w'CCGG_w
Cuts at: 0 1529 1865 2117 2273 2945 3264
Size: 1529 336 252 156 672 319
Fragments arranged by size:
1529 672 336 319 252 156
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 628 1477 2644 3264
Size: 628 849 1167 620
Fragments arranged by size:
1167 849 628 620
BseYI C'CCAG_C
Cuts at: 0 511 990 1207 1479 2512 2762 3264
Size: 511 479 217 272 1033 250 502
Fragments arranged by size:
1033 511 502 479 272 250 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 2421 3264
Size: 826 1595 843
Fragments arranged by size:
1595 843 826
BsiEI CG_ry'CG
Cuts at: 0 628 766 1310 3264
Size: 628 138 544 1954
Fragments arranged by size:
1954 628 544 138
BsiHKAI G_wGCw'C
Cuts at: 0 360 2297 2654 3104 3205 3264
Size: 360 1937 357 450 101 59
Fragments arranged by size:
1937 450 360 357 101 59
BsiWI C'GTAC_G
Cuts at: 0 629 3264
Size: 629 2635
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1748 3264
Size: 1748 1516
Bsp1286I G_dGCh'C
Cuts at: 0 277 360 485 580 671 732 932 997
Size: 277 83 125 95 91 61 200 65
Cuts at: 997 1208 1271 1379 1400 1540 1642 1652 2297
Size: 211 63 108 21 140 102 10 645
Cuts at: 2297 2513 2654 2824 3104 3205 3264
Size: 216 141 170 280 101 59
Fragments arranged by size:
645 280 277 216 211 200 170 141
140 125 108 102 101 95 91 83
65 63 61 59 21 10
BspEI T'CCGG_A
Cuts at: 0 2273 2945 3264
Size: 2273 672 319
Fragments arranged by size:
2273 672 319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 779 1571 3264
Size: 779 792 1693
Fragments arranged by size:
1693 792 779
BsrBI CCG'CTC
Cuts at: 0 560 3264
Size: 560 2704
BsrFI r'CCGG_y
Cuts at: 0 44 299 526 898 961 1110 3264
Size: 44 255 227 372 63 149 2154
Fragments arranged by size:
2154 372 255 227 149 63 44
BssHII G'CGCG_C
Cuts at: 0 749 1170 1172 3264
Size: 749 421 2 2092
Fragments arranged by size:
2092 749 421 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 54 309 447 1038 1320 1566 3264
Size: 54 255 138 591 282 246 1698
Fragments arranged by size:
1698 591 282 255 246 138 54
BstBI TT'CG_AA
Cuts at: 0 518 3264
Size: 518 2746
BstXI CCAn_nnnn'nTGG
Cuts at: 0 153 609 3264
Size: 153 456 2655
Fragments arranged by size:
2655 456 153
BstYI r'GATC_y
Cuts at: 0 1937 2397 3264
Size: 1937 460 867
Fragments arranged by size:
1937 867 460
Bsu36I CC'TnA_GG
Cuts at: 0 2095 2825 3264
Size: 2095 730 439
Fragments arranged by size:
2095 730 439
BtgI C'CryG_G
Cuts at: 0 602 622 663 968 1240 2987 3264
Size: 602 20 41 305 272 1747 277
Fragments arranged by size:
1747 602 305 277 272 41 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2298 2622 3264
Size: 2298 324 642
Fragments arranged by size:
2298 642 324
BtsI GCAGTG_nn'
Cuts at: 0 1111 3264
Size: 1111 2153
DraIII CAC_nnn'GTG
Cuts at: 0 1329 3264
Size: 1329 1935
EaeI y'GGCC_r
Cuts at: 0 351 625 672 763 1108 1307 1892 2159
Size: 351 274 47 91 345 199 585 267
Cuts at: 2159 2330 3264
Size: 171 934
Fragments arranged by size:
934 585 351 345 274 267 199 171
91 47
EagI C'GGCC_G
Cuts at: 0 625 763 1307 3264
Size: 625 138 544 1957
Fragments arranged by size:
1957 625 544 138
EarI CTCTTCn'nnn_
Cuts at: 0 1327 1754 1759 2713 3264
Size: 1327 427 5 954 551
Fragments arranged by size:
1327 954 551 427 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1241 1535 1712 2895 3264
Size: 1241 294 177 1183 369
Fragments arranged by size:
1241 1183 369 294 177
EcoICRI GAG'CTC
Cuts at: 0 358 3203 3264
Size: 358 2845 61
Fragments arranged by size:
2845 358 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 631 1177 1900 2581 2788 2901 2919
Size: 418 213 546 723 681 207 113 18
Cuts at: 2919 2920 3158 3260 3264
Size: 1 238 102 4
Fragments arranged by size:
723 681 546 418 238 213 207 113
102 18 4 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2161 3126 3264
Size: 2161 965 138
Fragments arranged by size:
2161 965 138
EcoO109I rG'GnC_Cy
Cuts at: 0 576 1375 1376 1639 2835 3264
Size: 576 799 1 263 1196 429
Fragments arranged by size:
1196 799 576 429 263 1
EcoRI G'AATT_C
Cuts at: 0 3115 3264
Size: 3115 149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2065 2373 3264
Size: 2065 308 891
Fragments arranged by size:
2065 891 308
FspI TGC'GCA
Cuts at: 0 1573 1611 3264
Size: 1573 38 1653
Fragments arranged by size:
1653 1573 38
HaeII r_GCGC'y
Cuts at: 0 593 610 899 1165 1480 1496 1682 2924
Size: 593 17 289 266 315 16 186 1242
Cuts at: 2924 3264
Size: 340
Fragments arranged by size:
1242 593 340 315 289 266 186 17
16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2518 2550 3264
Size: 2518 32 714
Fragments arranged by size:
2518 714 32
HincII GTy'rAC
Cuts at: 0 1863 3264
Size: 1863 1401
HindIII A'AGCT_T
Cuts at: 0 582 3185 3264
Size: 582 2603 79
Fragments arranged by size:
2603 582 79
HpaI GTT'AAC
Cuts at: 0 1863 3264
Size: 1863 1401
KasI G'GCGC_C
Cuts at: 0 895 1161 1492 3264
Size: 895 266 331 1772
Fragments arranged by size:
1772 895 331 266
KpnI G_GTAC'C
Cuts at: 0 566 3264
Size: 566 2698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1562 2676 3264
Size: 1562 1114 588
Fragments arranged by size:
1562 1114 588
MscI TGG'CCA
Cuts at: 0 1894 2332 3264
Size: 1894 438 932
Fragments arranged by size:
1894 932 438
MspA1I CmG'CkG
Cuts at: 0 286 555 624 741 970 981 1242 1503
Size: 286 269 69 117 229 11 261 261
Cuts at: 1503 1878 2766 3264
Size: 375 888 498
Fragments arranged by size:
888 498 375 286 269 261 261 229
117 69 11
NaeI GCC'GGC
Cuts at: 0 46 900 1112 3264
Size: 46 854 212 2152
Fragments arranged by size:
2152 854 212 46
NarI GG'CG_CC
Cuts at: 0 896 1162 1493 3264
Size: 896 266 331 1771
Fragments arranged by size:
1771 896 331 266
NcoI C'CATG_G
Cuts at: 0 2987 3264
Size: 2987 277
NgoMIV G'CCGG_C
Cuts at: 0 44 898 1110 3264
Size: 44 854 212 2154
Fragments arranged by size:
2154 854 212 44
NotI GC'GGCC_GC
Cuts at: 0 763 3264
Size: 763 2501
NspI r_CATG'y
Cuts at: 0 988 1471 3264
Size: 988 483 1793
Fragments arranged by size:
1793 988 483
PasI CC'CwG_GG
Cuts at: 0 792 2019 3264
Size: 792 1227 1245
Fragments arranged by size:
1245 1227 792
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 2352 2874 2988 3062 3264
Size: 345 2007 522 114 74 202
Fragments arranged by size:
2007 522 345 202 114 74
PmlI CAC'GTG
Cuts at: 0 1179 1827 3264
Size: 1179 648 1437
Fragments arranged by size:
1437 1179 648
PpuMI rG'GwC_Cy
Cuts at: 0 2835 3264
Size: 2835 429
PshAI GACnn'nnGTC
Cuts at: 0 1300 3264
Size: 1300 1964
PspOMI G'GGCC_C
Cuts at: 0 576 667 1375 1638 3264
Size: 576 91 708 263 1626
Fragments arranged by size:
1626 708 576 263 91
PstI C_TGCA'G
Cuts at: 0 285 291 314 746 1145 1877 3264
Size: 285 6 23 432 399 732 1387
Fragments arranged by size:
1387 732 432 399 285 23 6
PvuII CAG'CTG
Cuts at: 0 286 555 981 1878 2766 3264
Size: 286 269 426 897 888 498
Fragments arranged by size:
897 888 498 426 286 269
RsrII CG'GwC_CG
Cuts at: 0 1249 3264
Size: 1249 2015
SacI G_AGCT'C
Cuts at: 0 360 3205 3264
Size: 360 2845 59
Fragments arranged by size:
2845 360 59
SacII CC_GC'GG
Cuts at: 0 625 971 1243 3264
Size: 625 346 272 2021
Fragments arranged by size:
2021 625 346 272
SapI GCTCTTCn'nnn_
Cuts at: 0 1327 3264
Size: 1327 1937
SfcI C'TryA_G
Cuts at: 0 281 287 310 742 1141 1873 3264
Size: 281 6 23 432 399 732 1391
Fragments arranged by size:
1391 732 432 399 281 23 6
SfoI GGC'GCC
Cuts at: 0 897 1163 1494 3264
Size: 897 266 331 1770
Fragments arranged by size:
1770 897 331 266
SmlI C'TyrA_G
Cuts at: 0 228 361 3264
Size: 228 133 2903
Fragments arranged by size:
2903 228 133
SphI G_CATG'C
Cuts at: 0 1471 3264
Size: 1471 1793
SspI AAT'ATT
Cuts at: 0 2470 3264
Size: 2470 794
StuI AGG'CCT
Cuts at: 0 3061 3264
Size: 3061 203
StyI C'CwwG_G
Cuts at: 0 695 1457 1682 2987 3028 3056 3264
Size: 695 762 225 1305 41 28 208
Fragments arranged by size:
1305 762 695 225 208 41 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 704 708 959 1383 2169 3264
Size: 704 4 251 424 786 1095
Fragments arranged by size:
1095 786 704 424 251 4
TatI w'GTAC_w
Cuts at: 0 401 2222 3264
Size: 401 1821 1042
Fragments arranged by size:
1821 1042 401
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1591 1623 3264
Size: 1591 32 1641
Fragments arranged by size:
1641 1591 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 346 3264
Size: 346 2918
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BssHII BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MmeI MscI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PflMI PmlI
PpuMI PshAI PspOMI PstI PvuII RsrII SacI SacII
SapI SfcI SfoI SmlI SphI SspI StuI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BfrBI BmtI BsaBI BsaXI BsmI
BspHI BsrDI BsrGI BssSI BstEII BstZ17I ClaI CspCI
DraI DrdI EcoRV FseI FspAI MfeI MluI MslI
NdeI NheI NruI NsiI PacI PciI PfoI PmeI
PpiI PsiI PspXI PsrI PvuI SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI