Restriction map for
pFN21AE3363
(Linear) (Six-base) MAPSORT of: KIEE3363.seq Check: 6585 from: 1 to: 3210
KIEE3363.orf
With 182 enzymes: SgfI *
November 16, 2016 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1177 1996 3210
Size: 1177 819 1214
Fragments arranged by size:
1214 1177 819
AccI GT'mk_AC
Cuts at: 0 1106 1163 3210
Size: 1106 57 2047
Fragments arranged by size:
2047 1106 57
Acc65I G'GTAC_C
Cuts at: 0 79 1433 3210
Size: 79 1354 1777
Fragments arranged by size:
1777 1354 79
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1264 3144 3210
Size: 412 852 1880 66
Fragments arranged by size:
1880 852 412 66
AfeI AGC'GCT
Cuts at: 0 2185 3124 3210
Size: 2185 939 86
Fragments arranged by size:
2185 939 86
AflIII A'CryG_T
Cuts at: 0 183 3210
Size: 183 3027
AhdI GACnn_n'nnGTC
Cuts at: 0 1207 3210
Size: 1207 2003
AleI CACnn'nnGTG
Cuts at: 0 1533 3210
Size: 1533 1677
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 712 744 1354 1386 3210
Size: 712 32 610 32 1824
Fragments arranged by size:
1824 712 610 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 382 2520 2658 3210
Size: 382 2138 138 552
Fragments arranged by size:
2138 552 382 138
ApaI G_GGCC'C
Cuts at: 0 178 210 925 2077 3135 3210
Size: 178 32 715 1152 1058 75
Fragments arranged by size:
1152 1058 715 178 75 32
AvaI C'yCGr_G
Cuts at: 0 308 759 1673 1682 2215 2923 3085 3210
Size: 308 451 914 9 533 708 162 125
Fragments arranged by size:
914 708 533 451 308 162 125 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 172 205 1425 1458 3210
Size: 172 33 1220 33 1752
Fragments arranged by size:
1752 1220 172 33 33
BamHI G'GATC_C
Cuts at: 0 16 3210
Size: 16 3194
BanI G'GyrC_C
Cuts at: 0 79 137 289 461 687 1433 1563 2128
Size: 79 58 152 172 226 746 130 565
Cuts at: 2128 2901 3210
Size: 773 309
Fragments arranged by size:
773 746 565 309 226 172 152 130
79 58
BanII G_rGCy'C
Cuts at: 0 178 210 925 1055 2077 2320 2609 3135
Size: 178 32 715 130 1022 243 289 526
Cuts at: 3135 3210
Size: 75
Fragments arranged by size:
1022 715 526 289 243 178 130 75
32
BbeI G_GCGC'C
Cuts at: 0 141 3210
Size: 141 3069
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 974 1371 3210
Size: 90 884 397 1839
Fragments arranged by size:
1839 884 397 90
BbvCI CC'TCA_GC
Cuts at: 0 2277 2704 2851 3080 3136 3210
Size: 2277 427 147 229 56 74
Fragments arranged by size:
2277 427 229 147 74 56
BglI GCCn_nnn'nGGC
Cuts at: 0 1186 1239 2137 3210
Size: 1186 53 898 1073
Fragments arranged by size:
1186 1073 898 53
BlpI GC'TnA_GC
Cuts at: 0 49 2603 3210
Size: 49 2554 607
Fragments arranged by size:
2554 607 49
Bme1580I G_kGCm'C
Cuts at: 0 178 210 464 925 1235 1568 1746 2077
Size: 178 32 254 461 310 333 178 331
Cuts at: 2077 2820 3135 3210
Size: 743 315 75
Fragments arranged by size:
743 461 333 331 315 310 254 178
178 75 32
BmgBI CAC'GTC
Cuts at: 0 699 1959 3210
Size: 699 1260 1251
Fragments arranged by size:
1260 1251 699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1288 2956 3210
Size: 344 944 1668 254
Fragments arranged by size:
1668 944 344 254
Bpu10I CC'TnA_GC
Cuts at: 0 253 575 2277 2444 2704 2851 3080 3136
Size: 253 322 1702 167 260 147 229 56
Cuts at: 3136 3210
Size: 74
Fragments arranged by size:
1702 322 260 253 229 167 147 74
56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 669 1489 2632 3210
Size: 669 820 1143 578
Fragments arranged by size:
1143 820 669 578
BsaI GGTCTCn'nnnn_
Cuts at: 0 857 1844 2464 2636 3210
Size: 857 987 620 172 574
Fragments arranged by size:
987 857 620 574 172
BsaBI GATnn'nnATC
Cuts at: 0 1351 1924 3210
Size: 1351 573 1286
Fragments arranged by size:
1351 1286 573
BsaHI Gr'CG_yC
Cuts at: 0 138 3210
Size: 138 3072
BsaWI w'CCGG_w
Cuts at: 0 2528 3210
Size: 2528 682
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 712 742 2746 2776 3139 3169 3210
Size: 712 30 2004 30 363 30 41
Fragments arranged by size:
2004 712 363 41 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 733 1027 1285 2077 2239 2470 3210
Size: 733 294 258 792 162 231 740
Fragments arranged by size:
792 740 733 294 258 231 162
BseYI C'CCAG_C
Cuts at: 0 72 749 1054 1455 1523 1654 1908 2157
Size: 72 677 305 401 68 131 254 249
Cuts at: 2157 2403 3061 3128 3210
Size: 246 658 67 82
Fragments arranged by size:
677 658 401 305 254 249 246 131
82 72 68 67
BsiHKAI G_wGCw'C
Cuts at: 0 1073 2759 3210
Size: 1073 1686 451
Fragments arranged by size:
1686 1073 451
BsiWI C'GTAC_G
Cuts at: 0 1798 3210
Size: 1798 1412
BsmI GAATG_Cn'
Cuts at: 0 2245 3210
Size: 2245 965
Bsp1286I G_dGCh'C
Cuts at: 0 178 210 464 925 1055 1073 1235 1568
Size: 178 32 254 461 130 18 162 333
Cuts at: 1568 1746 2077 2320 2609 2759 2820 3135 3210
Size: 178 331 243 289 150 61 315 75
Fragments arranged by size:
461 333 331 315 289 254 243 178
178 162 150 130 75 61 32 18
BspEI T'CCGG_A
Cuts at: 0 2528 3210
Size: 2528 682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 367 1033 1177 1996 3210
Size: 367 666 144 819 1214
Fragments arranged by size:
1214 819 666 367 144
BsrBI CCG'CTC
Cuts at: 0 228 546 3210
Size: 228 318 2664
Fragments arranged by size:
2664 318 228
BsrDI GCAATG_nn'
Cuts at: 0 653 732 888 1709 1991 2147 3210
Size: 653 79 156 821 282 156 1063
Fragments arranged by size:
1063 821 653 282 156 156 79
BsrFI r'CCGG_y
Cuts at: 0 35 272 1459 3149 3210
Size: 35 237 1187 1690 61
Fragments arranged by size:
1690 1187 237 61 35
BsrGI T'GTAC_A
Cuts at: 0 2864 3210
Size: 2864 346
BssSI C'ACGA_G
Cuts at: 0 1120 3210
Size: 1120 2090
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2006 3210
Size: 2006 1204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 457 936 1908 2113 3180 3210
Size: 457 479 972 205 1067 30
Fragments arranged by size:
1067 972 479 457 205 30
BstYI r'GATC_y
Cuts at: 0 16 416 890 2285 2634 3210
Size: 16 400 474 1395 349 576
Fragments arranged by size:
1395 576 474 400 349 16
Bsu36I CC'TnA_GG
Cuts at: 0 1967 2659 3210
Size: 1967 692 551
Fragments arranged by size:
1967 692 551
BtgI C'CryG_G
Cuts at: 0 988 1095 1559 2327 2374 3018 3194 3210
Size: 988 107 464 768 47 644 176 16
Fragments arranged by size:
988 768 644 464 176 107 47 16
BtsI GCAGTG_nn'
Cuts at: 0 127 345 775 1540 1840 3210
Size: 127 218 430 765 300 1370
Fragments arranged by size:
1370 765 430 300 218 127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 822 857 3210
Size: 822 35 2353
Fragments arranged by size:
2353 822 35
EaeI y'GGCC_r
Cuts at: 0 23 562 618 874 906 1133 1178 1223
Size: 23 539 56 256 32 227 45 45
Cuts at: 1223 2330 3210
Size: 1107 880
Fragments arranged by size:
1107 880 539 256 227 56 45 45
32 23
EarI CTCTTCn'nnn_
Cuts at: 0 2404 2482 3210
Size: 2404 78 728
Fragments arranged by size:
2404 728 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2878 3210
Size: 2878 332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 412 1264 1288 2956 3144 3210
Size: 344 68 852 24 1668 188 66
Fragments arranged by size:
1668 852 344 188 68 66 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1303 2437 2854 3210
Size: 1303 1134 417 356
Fragments arranged by size:
1303 1134 417 356
EcoO109I rG'GnC_Cy
Cuts at: 0 206 207 379 497 921 1391 1658 1912
Size: 206 1 172 118 424 470 267 254
Cuts at: 1912 2028 2073 2074 3132 3210
Size: 116 45 1 1058 78
Fragments arranged by size:
1058 470 424 267 254 206 172 118
116 78 45 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 964 2385 3210
Size: 964 1421 825
Fragments arranged by size:
1421 964 825
FspI TGC'GCA
Cuts at: 0 1399 3210
Size: 1399 1811
HaeII r_GCGC'y
Cuts at: 0 141 148 2187 3126 3179 3210
Size: 141 7 2039 939 53 31
Fragments arranged by size:
2039 939 141 53 31 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1193 1225 2746 2778 3139 3171 3210
Size: 1193 32 1521 32 361 32 39
Fragments arranged by size:
1521 1193 361 39 32 32 32
HincII GTy'rAC
Cuts at: 0 1212 3210
Size: 1212 1998
HindIII A'AGCT_T
Cuts at: 0 666 3210
Size: 666 2544
KasI G'GCGC_C
Cuts at: 0 137 3210
Size: 137 3073
KpnI G_GTAC'C
Cuts at: 0 83 1437 3210
Size: 83 1354 1773
Fragments arranged by size:
1773 1354 83
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1742 2048 2519 2789 3210
Size: 1742 306 471 270 421
Fragments arranged by size:
1742 471 421 306 270
MscI TGG'CCA
Cuts at: 0 620 908 1135 1225 3210
Size: 620 288 227 90 1985
Fragments arranged by size:
1985 620 288 227 90
MslI CAynn'nnrTG
Cuts at: 0 455 1219 1533 2152 3210
Size: 455 764 314 619 1058
Fragments arranged by size:
1058 764 619 455 314
MspA1I CmG'CkG
Cuts at: 0 69 265 568 899 1003 1197 1266 1863
Size: 69 196 303 331 104 194 69 597
Cuts at: 1863 2006 2134 2329 2520 2907 3210
Size: 143 128 195 191 387 303
Fragments arranged by size:
597 387 331 303 303 196 195 194
191 143 128 104 69 69
NaeI GCC'GGC
Cuts at: 0 1461 3210
Size: 1461 1749
NarI GG'CG_CC
Cuts at: 0 138 3210
Size: 138 3072
NcoI C'CATG_G
Cuts at: 0 1095 3018 3210
Size: 1095 1923 192
Fragments arranged by size:
1923 1095 192
NgoMIV G'CCGG_C
Cuts at: 0 1459 3210
Size: 1459 1751
NspI r_CATG'y
Cuts at: 0 187 975 3210
Size: 187 788 2235
Fragments arranged by size:
2235 788 187
PasI CC'CwG_GG
Cuts at: 0 240 1845 2745 2844 3180 3210
Size: 240 1605 900 99 336 30
Fragments arranged by size:
1605 900 336 240 99 30
PciI A'CATG_T
Cuts at: 0 183 3210
Size: 183 3027
PflMI CCAn_nnn'nTGG
Cuts at: 0 303 699 1061 1125 1494 1959 2616 3210
Size: 303 396 362 64 369 465 657 594
Fragments arranged by size:
657 594 465 396 369 362 303 64
PfoI T'CCnGG_A
Cuts at: 0 215 935 1892 2933 3210
Size: 215 720 957 1041 277
Fragments arranged by size:
1041 957 720 277 215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 712 744 3210
Size: 712 32 2466
Fragments arranged by size:
2466 712 32
PpuMI rG'GwC_Cy
Cuts at: 0 379 497 1391 1658 1912 3210
Size: 379 118 894 267 254 1298
Fragments arranged by size:
1298 894 379 267 254 118
PshAI GACnn'nnGTC
Cuts at: 0 433 868 3210
Size: 433 435 2342
Fragments arranged by size:
2342 435 433
PspOMI G'GGCC_C
Cuts at: 0 174 206 921 2073 3131 3210
Size: 174 32 715 1152 1058 79
Fragments arranged by size:
1152 1058 715 174 79 32
PstI C_TGCA'G
Cuts at: 0 134 281 770 1835 2207 2390 2498 3210
Size: 134 147 489 1065 372 183 108 712
Fragments arranged by size:
1065 712 489 372 183 147 134 108
PvuII CAG'CTG
Cuts at: 0 265 568 1003 1266 2006 2520 3210
Size: 265 303 435 263 740 514 690
Fragments arranged by size:
740 690 514 435 303 265 263
SacII CC_GC'GG
Cuts at: 0 2330 3210
Size: 2330 880
SanDI GG'GwC_CC
Cuts at: 0 497 1658 1912 3210
Size: 497 1161 254 1298
Fragments arranged by size:
1298 1161 497 254
SapI GCTCTTCn'nnn_
Cuts at: 0 2482 3210
Size: 2482 728
ScaI AGT'ACT
Cuts at: 0 2878 3044 3210
Size: 2878 166 166
Fragments arranged by size:
2878 166 166
SfcI C'TryA_G
Cuts at: 0 130 277 766 1322 1831 2203 2386 2494
Size: 130 147 489 556 509 372 183 108
Cuts at: 2494 3210
Size: 716
Fragments arranged by size:
716 556 509 489 372 183 147 130
108
SfoI GGC'GCC
Cuts at: 0 139 3210
Size: 139 3071
SmaI CCC'GGG
Cuts at: 0 1675 3210
Size: 1675 1535
SmlI C'TyrA_G
Cuts at: 0 648 1504 1682 2647 3210
Size: 648 856 178 965 563
Fragments arranged by size:
965 856 648 563 178
SspI AAT'ATT
Cuts at: 0 1140 3210
Size: 1140 2070
StuI AGG'CCT
Cuts at: 0 2896 3210
Size: 2896 314
StyI C'CwwG_G
Cuts at: 0 1037 1095 3018 3143 3210
Size: 1037 58 1923 125 67
Fragments arranged by size:
1923 1037 125 67 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 942 1108 1629 2116 2749 2889 3210
Size: 942 166 521 487 633 140 321
Fragments arranged by size:
942 633 521 487 321 166 140
TatI w'GTAC_w
Cuts at: 0 188 2864 2876 3042 3210
Size: 188 2676 12 166 168
Fragments arranged by size:
2676 188 168 166 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 917 949 3210
Size: 917 32 2261
Fragments arranged by size:
2261 917 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 613 681 2400 2718 3210
Size: 613 68 1719 318 492
Fragments arranged by size:
1719 613 492 318 68
XhoI C'TCGA_G
Cuts at: 0 1682 3210
Size: 1682 1528
XmaI C'CCGG_G
Cuts at: 0 1673 3210
Size: 1673 1537
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AloI AlwNI ApaI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI CspCI EaeI EarI EciI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SacII SanDI SapI ScaI
SfcI SfoI SmaI SmlI SspI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AgeI AlfI ApaLI ApoI AscI
AseI AsiSI AvrII BcgI BciVI BclI BfrBI BglII
BmrI BmtI BplI BsaAI BsgI BsiEI BsmBI BspHI
BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII
DrdI EagI EcoICRI EcoRI EcoRV FseI FspAI HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI
SalI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XmnI ZraI