Restriction map for
pFN21AE3353
(Linear) (Six-base) MAPSORT of: KIEE3353.seq Check: 8875 from: 1 to: 3132
KIEE3353.orf
With 182 enzymes: SgfI *
July 3, 2012 17:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 220 714 1839 2198 3132
Size: 220 494 1125 359 934
Fragments arranged by size:
1125 934 494 359 220
AatII G_ACGT'C
Cuts at: 0 2396 3132
Size: 2396 736
AccI GT'mk_AC
Cuts at: 0 89 3132
Size: 89 3043
Acc65I G'GTAC_C
Cuts at: 0 541 3132
Size: 541 2591
AclI AA'CG_TT
Cuts at: 0 578 3132
Size: 578 2554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1737 2095 2246 2596 3055 3132
Size: 1737 358 151 350 459 77
Fragments arranged by size:
1737 459 358 350 151 77
AflII C'TTAA_G
Cuts at: 0 1294 3132
Size: 1294 1838
AflIII A'CryG_T
Cuts at: 0 1319 3110 3132
Size: 1319 1791 22
Fragments arranged by size:
1791 1319 22
AleI CACnn'nnGTG
Cuts at: 0 2659 3132
Size: 2659 473
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 811 1072 3132
Size: 811 261 2060
Fragments arranged by size:
2060 811 261
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 949 981 3132
Size: 949 32 2151
Fragments arranged by size:
2151 949 32
AlwNI CAG_nnn'CTG
Cuts at: 0 144 468 693 1122 1833 3132
Size: 144 324 225 429 711 1299
Fragments arranged by size:
1299 711 429 324 225 144
ApaI G_GGCC'C
Cuts at: 0 567 1911 3132
Size: 567 1344 1221
Fragments arranged by size:
1344 1221 567
ApaLI G'TGCA_C
Cuts at: 0 2244 3132
Size: 2244 888
ApoI r'AATT_y
Cuts at: 0 28 1891 2751 2854 3132
Size: 28 1863 860 103 278
Fragments arranged by size:
1863 860 278 103 28
AvaI C'yCGr_G
Cuts at: 0 193 1426 2589 3132
Size: 193 1233 1163 543
Fragments arranged by size:
1233 1163 543 193
BanI G'GyrC_C
Cuts at: 0 149 541 1946 2903 3132
Size: 149 392 1405 957 229
Fragments arranged by size:
1405 957 392 229 149
BanII G_rGCy'C
Cuts at: 0 518 567 980 1106 1911 2107 2966 3132
Size: 518 49 413 126 805 196 859 166
Fragments arranged by size:
859 805 518 413 196 166 126 49
BbeI G_GCGC'C
Cuts at: 0 2907 3132
Size: 2907 225
BbsI GAAGACnn'nnnn_
Cuts at: 0 716 2119 2842 3132
Size: 716 1403 723 290
Fragments arranged by size:
1403 723 716 290
BbvCI CC'TCA_GC
Cuts at: 0 124 3132
Size: 124 3008
BglI GCCn_nnn'nGGC
Cuts at: 0 1207 1775 3132
Size: 1207 568 1357
Fragments arranged by size:
1357 1207 568
BglII A'GATC_T
Cuts at: 0 1469 3132
Size: 1469 1663
BlpI GC'TnA_GC
Cuts at: 0 2101 2965 3132
Size: 2101 864 167
Fragments arranged by size:
2101 864 167
Bme1580I G_kGCm'C
Cuts at: 0 567 660 1372 1911 2248 3132
Size: 567 93 712 539 337 884
Fragments arranged by size:
884 712 567 539 337 93
BmgBI CAC'GTC
Cuts at: 0 273 633 3132
Size: 273 360 2499
Fragments arranged by size:
2499 360 273
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 3019 3132
Size: 153 2866 113
Fragments arranged by size:
2866 153 113
BmtI G_CTAG'C
Cuts at: 0 1639 3132
Size: 1639 1493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 402 2199 3132
Size: 402 1797 933
Fragments arranged by size:
1797 933 402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 532 976 1144 1483 1564 1759 2023
Size: 265 267 444 168 339 81 195 264
Cuts at: 2023 3132
Size: 1109
Fragments arranged by size:
1109 444 339 267 265 264 195 168
81
Bpu10I CC'TnA_GC
Cuts at: 0 124 1729 2960 3132
Size: 124 1605 1231 172
Fragments arranged by size:
1605 1231 172 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1840 2977 3132
Size: 1840 1137 155
Fragments arranged by size:
1840 1137 155
BsaAI yAC'GTr
Cuts at: 0 1773 1968 3132
Size: 1773 195 1164
Fragments arranged by size:
1773 1164 195
BsaHI Gr'CG_yC
Cuts at: 0 261 2393 2904 3132
Size: 261 2132 511 228
Fragments arranged by size:
2132 511 261 228
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 949 979 1093 1123 1762 1792 1939 1969
Size: 949 30 114 30 639 30 147 30
Cuts at: 1969 3132
Size: 1163
Fragments arranged by size:
1163 949 639 147 114 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 346 457 613 828 1528 1531 2311
Size: 58 288 111 156 215 700 3 780
Cuts at: 2311 2632 3037 3040 3132
Size: 321 405 3 92
Fragments arranged by size:
780 700 405 321 288 215 156 111
92 58 3 3
BseYI C'CCAG_C
Cuts at: 0 468 473 1479 2209 2664 3132
Size: 468 5 1006 730 455 468
Fragments arranged by size:
1006 730 468 468 455 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 781 1048 1867 3132
Size: 295 486 267 819 1265
Fragments arranged by size:
1265 819 486 295 267
BsiEI CG_ry'CG
Cuts at: 0 321 452 2679 3132
Size: 321 131 2227 453
Fragments arranged by size:
2227 453 321 131
BsiHKAI G_wGCw'C
Cuts at: 0 980 1099 1577 1622 1708 1856 2107 2248
Size: 980 119 478 45 86 148 251 141
Cuts at: 2248 2966 3132
Size: 718 166
Fragments arranged by size:
980 718 478 251 166 148 141 119
86 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1171 3132
Size: 1171 1961
Bsp1286I G_dGCh'C
Cuts at: 0 518 567 660 980 1099 1106 1372 1577
Size: 518 49 93 320 119 7 266 205
Cuts at: 1577 1622 1708 1856 1911 2107 2248 2966 3132
Size: 45 86 148 55 196 141 718 166
Fragments arranged by size:
718 518 320 266 205 196 166 148
141 119 93 86 55 49 45 7
BspHI T'CATG_A
Cuts at: 0 2696 3132
Size: 2696 436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 220 714 1157 1706 1839 1841 2198 3132
Size: 220 494 443 549 133 2 357 934
Fragments arranged by size:
934 549 494 443 357 220 133 2
BsrBI CCG'CTC
Cuts at: 0 171 765 1011 1548 1955 3132
Size: 171 594 246 537 407 1177
Fragments arranged by size:
1177 594 537 407 246 171
BsrDI GCAATG_nn'
Cuts at: 0 120 1250 3132
Size: 120 1130 1882
Fragments arranged by size:
1882 1130 120
BsrFI r'CCGG_y
Cuts at: 0 458 3132
Size: 458 2674
BsrGI T'GTAC_A
Cuts at: 0 2285 3132
Size: 2285 847
BssSI C'ACGA_G
Cuts at: 0 376 1576 3132
Size: 376 1200 1556
Fragments arranged by size:
1556 1200 376
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1845 2361 3132
Size: 1845 516 771
Fragments arranged by size:
1845 771 516
BstEII G'GTnAC_C
Cuts at: 0 1653 3132
Size: 1653 1479
BstYI r'GATC_y
Cuts at: 0 1469 3132
Size: 1469 1663
Bsu36I CC'TnA_GG
Cuts at: 0 2422 3132
Size: 2422 710
BtgI C'CryG_G
Cuts at: 0 23 824 1044 1364 2907 3132
Size: 23 801 220 320 1543 225
Fragments arranged by size:
1543 801 320 225 220 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2484 3132
Size: 2484 648
BtsI GCAGTG_nn'
Cuts at: 0 2248 3132
Size: 2248 884
DraIII CAC_nnn'GTG
Cuts at: 0 1446 1661 3111 3132
Size: 1446 215 1450 21
Fragments arranged by size:
1450 1446 215 21
EaeI y'GGCC_r
Cuts at: 0 449 1711 1913 1929 2651 2736 3132
Size: 449 1262 202 16 722 85 396
Fragments arranged by size:
1262 722 449 396 202 85 16
EagI C'GGCC_G
Cuts at: 0 449 3132
Size: 449 2683
EarI CTCTTCn'nnn_
Cuts at: 0 178 2923 3132
Size: 178 2745 209
Fragments arranged by size:
2745 209 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1255 3132
Size: 1255 1877
EcoICRI GAG'CTC
Cuts at: 0 978 2105 2964 3132
Size: 978 1127 859 168
Fragments arranged by size:
1127 978 859 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 532 976 1144 1483 1564 1737 1759
Size: 265 267 444 168 339 81 173 22
Cuts at: 1759 2023 2095 2246 2596 3055 3132
Size: 264 72 151 350 459 77
Fragments arranged by size:
459 444 350 339 267 265 264 173
168 151 81 77 72 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1426 2013 2353 2473 3132
Size: 1426 587 340 120 659
Fragments arranged by size:
1426 659 587 340 120
EcoO109I rG'GnC_Cy
Cuts at: 0 535 564 1584 1908 2079 2178 2598 2885
Size: 535 29 1020 324 171 99 420 287
Cuts at: 2885 3132
Size: 247
Fragments arranged by size:
1020 535 420 324 287 247 171 99
29
EcoRI G'AATT_C
Cuts at: 0 28 3132
Size: 28 3104
FspI TGC'GCA
Cuts at: 0 1378 3132
Size: 1378 1754
HaeII r_GCGC'y
Cuts at: 0 646 1403 1636 1736 2907 3132
Size: 646 757 233 100 1171 225
Fragments arranged by size:
1171 757 646 233 225 100
HincII GTy'rAC
Cuts at: 0 66 576 809 3132
Size: 66 510 233 2323
Fragments arranged by size:
2323 510 233 66
KasI G'GCGC_C
Cuts at: 0 2903 3132
Size: 2903 229
KpnI G_GTAC'C
Cuts at: 0 545 3132
Size: 545 2587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2535 3132
Size: 2535 597
MscI TGG'CCA
Cuts at: 0 1915 2653 2738 3132
Size: 1915 738 85 394
Fragments arranged by size:
1915 738 394 85
MslI CAynn'nnrTG
Cuts at: 0 1676 2038 2251 2476 2659 3132
Size: 1676 362 213 225 183 473
Fragments arranged by size:
1676 473 362 225 213 183
MspA1I CmG'CkG
Cuts at: 0 141 229 468 510 639 693 791 1119
Size: 141 88 239 42 129 54 98 328
Cuts at: 1119 1405 1524 1530 1842 2309 2718 2969 3132
Size: 286 119 6 312 467 409 251 163
Fragments arranged by size:
467 409 328 312 286 251 239 163
141 129 119 98 88 54 42 6
NaeI GCC'GGC
Cuts at: 0 460 3132
Size: 460 2672
NarI GG'CG_CC
Cuts at: 0 2904 3132
Size: 2904 228
NcoI C'CATG_G
Cuts at: 0 23 824 3132
Size: 23 801 2308
Fragments arranged by size:
2308 801 23
NdeI CA'TA_TG
Cuts at: 0 2658 3132
Size: 2658 474
NgoMIV G'CCGG_C
Cuts at: 0 458 3132
Size: 458 2674
NheI G'CTAG_C
Cuts at: 0 1635 3132
Size: 1635 1497
NotI GC'GGCC_GC
Cuts at: 0 449 3132
Size: 449 2683
NspI r_CATG'y
Cuts at: 0 183 1323 1507 2157 2364 3132
Size: 183 1140 184 650 207 768
Fragments arranged by size:
1140 768 650 207 184 183
PasI CC'CwG_GG
Cuts at: 0 7 3132
Size: 7 3125
PciI A'CATG_T
Cuts at: 0 1319 3132
Size: 1319 1813
PflMI CCAn_nnn'nTGG
Cuts at: 0 1256 2388 2745 3132
Size: 1256 1132 357 387
Fragments arranged by size:
1256 1132 387 357
PfoI T'CCnGG_A
Cuts at: 0 1541 1613 1625 2000 3132
Size: 1541 72 12 375 1132
Fragments arranged by size:
1541 1132 375 72 12
PmlI CAC'GTG
Cuts at: 0 1968 3132
Size: 1968 1164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 949 981 1298 1330 3132
Size: 949 32 317 32 1802
Fragments arranged by size:
1802 949 317 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1584 3132
Size: 1584 1548
PshAI GACnn'nnGTC
Cuts at: 0 826 3132
Size: 826 2306
PspOMI G'GGCC_C
Cuts at: 0 563 1907 3132
Size: 563 1344 1225
Fragments arranged by size:
1344 1225 563
PstI C_TGCA'G
Cuts at: 0 491 509 725 790 1529 1841 2017 2195
Size: 491 18 216 65 739 312 176 178
Cuts at: 2195 2375 3132
Size: 180 757
Fragments arranged by size:
757 739 491 312 216 180 178 176
65 18
PvuI CG_AT'CG
Cuts at: 0 2679 3132
Size: 2679 453
PvuII CAG'CTG
Cuts at: 0 141 468 510 791 1524 1530 1842 2718
Size: 141 327 42 281 733 6 312 876
Cuts at: 2718 2969 3132
Size: 251 163
Fragments arranged by size:
876 733 327 312 281 251 163 141
42 6
SacI G_AGCT'C
Cuts at: 0 980 2107 2966 3132
Size: 980 1127 859 166
Fragments arranged by size:
1127 980 859 166
SapI GCTCTTCn'nnn_
Cuts at: 0 178 3132
Size: 178 2954
SexAI A'CCwGG_T
Cuts at: 0 1586 3132
Size: 1586 1546
SfcI C'TryA_G
Cuts at: 0 487 505 721 786 1525 1837 2013 2191
Size: 487 18 216 65 739 312 176 178
Cuts at: 2191 2371 3132
Size: 180 761
Fragments arranged by size:
761 739 487 312 216 180 178 176
65 18
SfoI GGC'GCC
Cuts at: 0 2905 3132
Size: 2905 227
SmaI CCC'GGG
Cuts at: 0 2591 3132
Size: 2591 541
SmlI C'TyrA_G
Cuts at: 0 1294 1855 2992 3132
Size: 1294 561 1137 140
Fragments arranged by size:
1294 1137 561 140
SphI G_CATG'C
Cuts at: 0 2157 3132
Size: 2157 975
StuI AGG'CCT
Cuts at: 0 199 371 481 2008 2167 2468 2954 3132
Size: 199 172 110 1527 159 301 486 178
Fragments arranged by size:
1527 486 301 199 178 172 159 110
StyI C'CwwG_G
Cuts at: 0 23 38 626 824 2132 3132
Size: 23 15 588 198 1308 1000
Fragments arranged by size:
1308 1000 588 198 23 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2041 3132
Size: 2041 1091
TatI w'GTAC_w
Cuts at: 0 491 779 1448 2285 3132
Size: 491 288 669 837 847
Fragments arranged by size:
847 837 669 491 288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1093 1125 1576 1608 1762 1794 1939 1971
Size: 1093 32 451 32 154 32 145 32
Cuts at: 1971 2179 2211 2383 2415 3097 3129 3132
Size: 208 32 172 32 682 32 3
Fragments arranged by size:
1093 682 451 208 172 154 145 32
32 32 32 32 32 32 3
Tth111I GACn'n_nGTC
Cuts at: 0 1071 2092 2812 3132
Size: 1071 1021 720 320
Fragments arranged by size:
1071 1021 720 320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 184 1896 2217 2661 2731 3132
Size: 184 1712 321 444 70 401
Fragments arranged by size:
1712 444 401 321 184 70
XmaI C'CCGG_G
Cuts at: 0 2589 3132
Size: 2589 543
XmnI GAAnn'nnTTC
Cuts at: 0 2089 3040 3132
Size: 2089 951 92
Fragments arranged by size:
2089 951 92
ZraI GAC'GTC
Cuts at: 0 2394 3132
Size: 2394 738
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII SacI
SapI SexAI SfcI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AgeI AhdI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BsaI BsaBI BsaWI
BsiWI BsmI BspEI BssHII BstBI BstXI BstZ17I ClaI
CspCI DraI DrdI EcoRV FalI FseI FspAI Hin4I
HindIII HpaI MfeI MluI NruI NsiI PacI PmeI
PsiI PspXI PsrI RsrII SacII SalI SanDI SbfI
ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI