Restriction map for
pFN21AE3351
(Linear) (Six-base) MAPSORT of: KIEE3351.seq Check: 5767 from: 1 to: 3126
KIEE3351.orf
With 182 enzymes: SgfI *
July 3, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 165 3126
Size: 165 2961
AccI GT'mk_AC
Cuts at: 0 1917 3126
Size: 1917 1209
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 721 1943 2132 2462 3126
Size: 352 369 1222 189 330 664
Fragments arranged by size:
1222 664 369 352 330 189
AflIII A'CryG_T
Cuts at: 0 1357 1883 3126
Size: 1357 526 1243
Fragments arranged by size:
1357 1243 526
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 446 1490 3126
Size: 446 1044 1636
Fragments arranged by size:
1636 1044 446
AlwNI CAG_nnn'CTG
Cuts at: 0 192 2155 2485 3126
Size: 192 1963 330 641
Fragments arranged by size:
1963 641 330 192
ApaI G_GGCC'C
Cuts at: 0 2997 3126
Size: 2997 129
ApoI r'AATT_y
Cuts at: 0 1658 2336 3126
Size: 1658 678 790
Fragments arranged by size:
1658 790 678
AseI AT'TA_AT
Cuts at: 0 1450 3126
Size: 1450 1676
AvaI C'yCGr_G
Cuts at: 0 2984 2990 3126
Size: 2984 6 136
Fragments arranged by size:
2984 136 6
AvrII C'CTAG_G
Cuts at: 0 1347 3126
Size: 1347 1779
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 851 884 3126
Size: 851 33 2242
Fragments arranged by size:
2242 851 33
BamHI G'GATC_C
Cuts at: 0 774 3126
Size: 774 2352
BanI G'GyrC_C
Cuts at: 0 182 2511 3126
Size: 182 2329 615
Fragments arranged by size:
2329 615 182
BanII G_rGCy'C
Cuts at: 0 2991 2997 3126
Size: 2991 6 129
Fragments arranged by size:
2991 129 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1753 3126
Size: 1753 1373
BciVI GTATCCnnnnn_n'
Cuts at: 0 852 3126
Size: 852 2274
BclI T'GATC_A
Cuts at: 0 2310 3126
Size: 2310 816
BfrBI ATG'CAT
Cuts at: 0 470 685 3126
Size: 470 215 2441
Fragments arranged by size:
2441 470 215
BglI GCCn_nnn'nGGC
Cuts at: 0 1483 3126
Size: 1483 1643
BglII A'GATC_T
Cuts at: 0 1176 1622 1842 3126
Size: 1176 446 220 1284
Fragments arranged by size:
1284 1176 446 220
BlpI GC'TnA_GC
Cuts at: 0 848 1015 3126
Size: 848 167 2111
Fragments arranged by size:
2111 848 167
Bme1580I G_kGCm'C
Cuts at: 0 185 435 2516 2997 3126
Size: 185 250 2081 481 129
Fragments arranged by size:
2081 481 250 185 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 1217 1334 1907 2681 3126
Size: 1217 117 573 774 445
Fragments arranged by size:
1217 774 573 445 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 169 214 1016 1216 2165 3126
Size: 34 135 45 802 200 949 961
Fragments arranged by size:
961 949 802 200 135 45 34
Bpu10I CC'TnA_GC
Cuts at: 0 1217 3126
Size: 1217 1909
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 3126
Size: 34 3092
BsaI GGTCTCn'nnnn_
Cuts at: 0 972 1714 2897 3126
Size: 972 742 1183 229
Fragments arranged by size:
1183 972 742 229
BsaHI Gr'CG_yC
Cuts at: 0 1288 3126
Size: 1288 1838
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2667 2697 3126
Size: 2667 30 429
Fragments arranged by size:
2667 429 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3107 3126
Size: 3107 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 2484 2711 3126
Size: 742 1742 227 415
Fragments arranged by size:
1742 742 415 227
BsiHKAI G_wGCw'C
Cuts at: 0 92 990 3126
Size: 92 898 2136
Fragments arranged by size:
2136 898 92
BsmI GAATG_Cn'
Cuts at: 0 1026 1672 2985 3126
Size: 1026 646 1313 141
Fragments arranged by size:
1313 1026 646 141
Bsp1286I G_dGCh'C
Cuts at: 0 92 185 435 990 2516 2991 2997 3126
Size: 92 93 250 555 1526 475 6 129
Fragments arranged by size:
1526 555 475 250 129 93 92 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 165 372 2792 3126
Size: 165 207 2420 334
Fragments arranged by size:
2420 334 207 165
BsrDI GCAATG_nn'
Cuts at: 0 472 3126
Size: 472 2654
BsrFI r'CCGG_y
Cuts at: 0 215 3126
Size: 215 2911
BstAPI GCAn_nnn'nTGC
Cuts at: 0 42 189 3126
Size: 42 147 2937
Fragments arranged by size:
2937 147 42
BstBI TT'CG_AA
Cuts at: 0 516 3126
Size: 516 2610
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1284 3126
Size: 1284 1842
BstYI r'GATC_y
Cuts at: 0 8 363 774 1176 1622 1842 1896 3126
Size: 8 355 411 402 446 220 54 1230
Fragments arranged by size:
1230 446 411 402 355 220 54 8
BstZ17I GTA'TAC
Cuts at: 0 1918 3126
Size: 1918 1208
BtgI C'CryG_G
Cuts at: 0 1543 3126
Size: 1543 1583
BtsI GCAGTG_nn'
Cuts at: 0 115 1677 2353 3126
Size: 115 1562 676 773
Fragments arranged by size:
1562 773 676 115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2348 2383 3126
Size: 2348 35 743
Fragments arranged by size:
2348 743 35
DraI TTT'AAA
Cuts at: 0 1427 3126
Size: 1427 1699
DraIII CAC_nnn'GTG
Cuts at: 0 189 3126
Size: 189 2937
EaeI y'GGCC_r
Cuts at: 0 217 838 1394 3126
Size: 217 621 556 1732
Fragments arranged by size:
1732 621 556 217
EarI CTCTTCn'nnn_
Cuts at: 0 678 740 1223 1875 2406 2613 3126
Size: 678 62 483 652 531 207 513
Fragments arranged by size:
678 652 531 513 483 207 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 100 3126
Size: 100 3026
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 169 214 352 721 1016 1216 1943
Size: 34 135 45 138 369 295 200 727
Cuts at: 1943 2132 2165 2462 3126
Size: 189 33 297 664
Fragments arranged by size:
727 664 369 297 295 200 189 138
135 45 34 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 268 379 3126
Size: 268 111 2747
Fragments arranged by size:
2747 268 111
EcoO109I rG'GnC_Cy
Cuts at: 0 18 159 2994 3126
Size: 18 141 2835 132
Fragments arranged by size:
2835 141 132 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 689 2408 3126
Size: 689 1719 718
Fragments arranged by size:
1719 718 689
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 817 849 1041 1073 1888 1920
Size: 214 32 571 32 192 32 815 32
Cuts at: 1920 2335 2367 2667 2699 3126
Size: 415 32 300 32 427
Fragments arranged by size:
815 571 427 415 300 214 192 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 505 2433 3126
Size: 505 1928 693
Fragments arranged by size:
1928 693 505
HindIII A'AGCT_T
Cuts at: 0 2492 3126
Size: 2492 634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2008 3126
Size: 2008 1118
MscI TGG'CCA
Cuts at: 0 840 1396 3126
Size: 840 556 1730
Fragments arranged by size:
1730 840 556
MspA1I CmG'CkG
Cuts at: 0 171 510 895 1993 3126
Size: 171 339 385 1098 1133
Fragments arranged by size:
1133 1098 385 339 171
NcoI C'CATG_G
Cuts at: 0 1543 3126
Size: 1543 1583
NdeI CA'TA_TG
Cuts at: 0 2307 3126
Size: 2307 819
NsiI A_TGCA'T
Cuts at: 0 472 687 3126
Size: 472 215 2439
Fragments arranged by size:
2439 472 215
NspI r_CATG'y
Cuts at: 0 856 1011 1361 1887 3126
Size: 856 155 350 526 1239
Fragments arranged by size:
1239 856 526 350 155
PciI A'CATG_T
Cuts at: 0 1357 1883 3126
Size: 1357 526 1243
Fragments arranged by size:
1357 1243 526
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 285 2884 3079 3126
Size: 192 93 2599 195 47
Fragments arranged by size:
2599 195 192 93 47
PfoI T'CCnGG_A
Cuts at: 0 11 146 308 3126
Size: 11 135 162 2818
Fragments arranged by size:
2818 162 135 11
PpuMI rG'GwC_Cy
Cuts at: 0 159 3126
Size: 159 2967
PsiI TTA'TAA
Cuts at: 0 1979 3126
Size: 1979 1147
PspOMI G'GGCC_C
Cuts at: 0 2993 3126
Size: 2993 133
PspXI vC'TCGA_Gb
Cuts at: 0 2984 3126
Size: 2984 142
PstI C_TGCA'G
Cuts at: 0 383 608 1810 3126
Size: 383 225 1202 1316
Fragments arranged by size:
1316 1202 383 225
PvuII CAG'CTG
Cuts at: 0 510 895 1993 3126
Size: 510 385 1098 1133
Fragments arranged by size:
1133 1098 510 385
SbfI CC_TGCA'GG
Cuts at: 0 383 3126
Size: 383 2743
ScaI AGT'ACT
Cuts at: 0 1712 2853 3126
Size: 1712 1141 273
Fragments arranged by size:
1712 1141 273
SexAI A'CCwGG_T
Cuts at: 0 185 981 3126
Size: 185 796 2145
Fragments arranged by size:
2145 796 185
SfcI C'TryA_G
Cuts at: 0 379 604 701 1806 2423 2914 3126
Size: 379 225 97 1105 617 491 212
Fragments arranged by size:
1105 617 491 379 225 212 97
SmaI CCC'GGG
Cuts at: 0 2992 3126
Size: 2992 134
SmlI C'TyrA_G
Cuts at: 0 49 2984 3126
Size: 49 2935 142
Fragments arranged by size:
2935 142 49
SrfI GCCC'GGGC
Cuts at: 0 2992 3126
Size: 2992 134
SspI AAT'ATT
Cuts at: 0 2209 3126
Size: 2209 917
StuI AGG'CCT
Cuts at: 0 199 1639 3126
Size: 199 1440 1487
Fragments arranged by size:
1487 1440 199
StyI C'CwwG_G
Cuts at: 0 476 1347 1543 3126
Size: 476 871 196 1583
Fragments arranged by size:
1583 871 476 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 469 562 1926 3126
Size: 469 93 1364 1200
Fragments arranged by size:
1364 1200 469 93
TatI w'GTAC_w
Cuts at: 0 1108 1122 1710 2599 2851 3126
Size: 1108 14 588 889 252 275
Fragments arranged by size:
1108 889 588 275 252 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 390 422 3126
Size: 390 32 2704
Fragments arranged by size:
2704 390 32
XbaI T'CTAG_A
Cuts at: 0 1300 3126
Size: 1300 1826
XhoI C'TCGA_G
Cuts at: 0 2984 3126
Size: 2984 142
XmaI C'CCGG_G
Cuts at: 0 2990 3126
Size: 2990 136
XmnI GAAnn'nnTTC
Cuts at: 0 1190 2391 3126
Size: 1190 1201 735
Fragments arranged by size:
1201 1190 735
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaI ApoI
AseI AvaI AvrII BaeI BamHI BanI BanII BbsI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtsI CspCI DraI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI Hin4I
HincII HindIII MmeI MscI MspA1I NcoI NdeI NsiI
NspI PciI PflMI PfoI PpuMI PsiI PspOMI PspXI
PstI PvuII SbfI ScaI SexAI SfcI SmaI SmlI
SrfI SspI StuI StyI TaqII TatI TstI XbaI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AsiSI BbeI BbvCI BcgI BmgBI
BmtI BplI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstEII
Bsu36I BtgZI ClaI DrdI EagI EcoICRI EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PmeI PmlI PpiI PshAI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SwaI Tth111I XcmI
ZraI